Adam3 ADAM metallopeptidase domain 3 [Mus musculus (house mouse)] - Gene

Summary

Official Symbol: Adam3provided by MGI
Official Full Name: ADAM metallopeptidase domain 3provided by MGI
Primary source: MGI:MGI:102518
See related: Ensembl:ENSMUSG00000031553 AllianceGenome:MGI:102518
Gene type: protein coding
RefSeq status: VALIDATED
Organism: Mus musculus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
Also known as: tMDC; Cyrn1; Taz83
Summary: Predicted to enable metalloendopeptidase activity. Involved in fertilization and flagellated sperm motility. Acts upstream of or within binding activity of sperm to zona pellucida; positive regulation of gene expression; and regulation of mRNA processing. Located in several cellular components, including cell surface; membrane raft; and sperm head. Part of protein-containing complex. Human ortholog(s) of this gene implicated in brain glioma and conjunctival squamous cell carcinoma. Orthologous to human ADAM3A (ADAM metallopeptidase domain 3A (pseudogene)). [provided by Alliance of Genome Resources, Apr 2022]
Expression: Restricted expression toward testis adult (RPKM 218.4) See more
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Genomic context

Location:: 8 A2; 8 13.09 cM

Exon count:: 23

Annotation release	Status	Assembly	Chr	Location
RS_2024_02	current	GRCm39 (GCF_000001635.27)	8	NC_000074.7 (25167248..25216393, complement)
108.20200622	previous assembly	GRCm38.p6 (GCF_000001635.26)	8	NC_000074.6 (24677232..24726377, complement)

Chromosome 8 - NC_000074.7 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Expression

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See details

Project title: Mouse ENCODE transcriptome data
Description: RNA profiling data sets generated by the Mouse ENCODE project.
BioProject: PRJNA66167
Publication: PMID 25409824
Analysis date: n/a

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Dissecting the PRSS37 interactome and potential mechanisms leading to ADAM3 loss in PRSS37-null sperm.
Title: Dissecting the PRSS37 interactome and potential mechanisms leading to ADAM3 loss in PRSS37-null sperm.
co-expression of sperm membrane proteins CMTM2A and CMTM2B is required for male fertility and affects the localization of the sperm membrane protein ADAM3 in regulating sperm fertilizing ability.
Title: Co-expression of sperm membrane proteins CMTM2A and CMTM2B is essential for ADAM3 localization and male fertility in mice.
Protein disulfide isomerase homolog PDILT is required for quality control of sperm membrane protein ADAM3 and male infertility
Title: Protein disulfide isomerase homolog PDILT is required for quality control of sperm membrane protein ADAM3 and male fertility [corrected].
Lack of tyrosylprotein sulfotransferase-2 activity results in altered sperm-egg interactions and loss of ADAM3 and ADAM6 in epididymal sperm.
Title: Lack of tyrosylprotein sulfotransferase-2 activity results in altered sperm-egg interactions and loss of ADAM3 and ADAM6 in epididymal sperm.
we report that Adam2 and Adam3 knockout sperm have severely impaired sperm aggregation and that this defect is not restored over time during in vitro cultivation.
Title: Impaired sperm aggregation in Adam2 and Adam3 null mice.
The Adam3-disrupted sperm were unable to migrate into the oviduct after coitus.
Title: Disruption of ADAM3 impairs the migration of sperm into oviduct in mouse.
Results suggest that association with ADAM2 is a key element for stability of ADAM3 in epididymal sperm, and the presence of the ADAM2-ADAM3 complex in sperm also suggests a potential role of ADAM2 with ADAM3 in sperm binding to the egg zona pellucida.
Title: Identification of an ADAM2-ADAM3 complex on the surface of mouse testicular germ cells and cauda epididymal sperm.
ADAM3 expression is regulated by ADAM1a/ADAM2 Fertilin complex on the cell surface of mouse sperm
Title: Possible function of the ADAM1a/ADAM2 Fertilin complex in the appearance of ADAM3 on the sperm surface.
the mature form of ADAM3 is involved in the binding of sperm to the egg zona pellucida, not in the membrane fusion between sperm and egg.
Title: Synthesis, processing, and subcellular localization of mouse ADAM3 during spermatogenesis and epididymal sperm transport.
Angiotensin-converting enzyme is involved in distributing ADAM3 to a location where it can participate in sperm-zona pellucida binding.
Title: Aberrant distribution of ADAM3 in sperm from both angiotensin-converting enzyme (Ace)- and calmegin (Clgn)-deficient mice.

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Variation

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Alleles

Alleles of this type are documented at Mouse Genome Informatics (MGI)

Targeted (4) 1 citation
Endonuclease-mediated (2)

General gene information

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Gene Ontology Provided by MGI

Function	Evidence Code	Pubs
enables metalloendopeptidase activity	IBA Inferred from Biological aspect of Ancestor more info
enables metallopeptidase activity	IEA Inferred from Electronic Annotation more info
enables protein binding	IPI Inferred from Physical Interaction more info	PubMed

Process	Evidence Code	Pubs
involved_in binding of sperm to zona pellucida	IBA Inferred from Biological aspect of Ancestor more info
acts_upstream_of_or_within binding of sperm to zona pellucida	IDA Inferred from Direct Assay more info	PubMed
acts_upstream_of_or_within binding of sperm to zona pellucida	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in cell adhesion	IBA Inferred from Biological aspect of Ancestor more info
involved_in fertilization	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in flagellated sperm motility	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in male gonad development	IBA Inferred from Biological aspect of Ancestor more info
acts_upstream_of_or_within positive regulation of gene expression	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in proteolysis	IBA Inferred from Biological aspect of Ancestor more info
acts_upstream_of_or_within regulation of mRNA processing	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within single fertilization	IGI Inferred from Genetic Interaction more info	PubMed

Component	Evidence Code	Pubs
located_in cell surface	IDA Inferred from Direct Assay more info	PubMed
located_in membrane	IEA Inferred from Electronic Annotation more info
located_in membrane raft	IDA Inferred from Direct Assay more info	PubMed
is_active_in plasma membrane	IBA Inferred from Biological aspect of Ancestor more info
located_in plasma membrane	IDA Inferred from Direct Assay more info	PubMed
part_of protein-containing complex	IDA Inferred from Direct Assay more info	PubMed
located_in sperm head	IDA Inferred from Direct Assay more info	PubMed

General protein information

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Preferred Names: A disintegrin and metallopeptidase domain 3

Names: a disintegrin and metallopeptidase domain 3 (cyritestin)

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_001324489.1 → NP_001311418.1 A disintegrin and metallopeptidase domain 3 isoform 2

Status: VALIDATED

Source sequence(s)

AC138355, BC049609, CJ047279, X64227

UniProtKB/TrEMBL

Q62287, Q810R6

Conserved Domains (4) summary

smart00608
Location:289 → 420

ACR; ADAM Cysteine-Rich Domain

pfam00200
Location:210 → 285

Disintegrin

pfam01421
Location:1 → 188

Reprolysin; Reprolysin (M12B) family zinc metalloprotease

pfam01683
Location:406 → 455

EB; EB module
NM_009619.5 → NP_033749.2 A disintegrin and metallopeptidase domain 3 isoform 1 precursor

See identical proteins and their annotated locations for NP_033749.2

Status: VALIDATED

Source sequence(s)

AC138355, BC049609, CJ047279, X64227

Consensus CDS

CCDS22196.1

UniProtKB/Swiss-Prot

F8VQ03

UniProtKB/TrEMBL

Q62287

Related

ENSMUSP00000033958.8, ENSMUST00000033958.15

Conserved Domains (4) summary

smart00050
Location:404 → 480

DISIN; Homologues of snake disintegrins

smart00608
Location:483 → 614

ACR; ADAM Cysteine-Rich Domain

pfam01562
Location:27 → 144

Pep_M12B_propep; Reprolysin family propeptide

cl26139
Location:187 → 382

Reprolysin; Reprolysin (M12B) family zinc metalloprotease

RefSeqs of Annotated Genomes: GCF_000001635.27-RS_2024_02

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCm39 C57BL/6J

Genomic

NC_000074.7 Reference GRCm39 C57BL/6J

Range

25167248..25216393 complement

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GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

XM_011242113.3 → XP_011240415.1 A disintegrin and metallopeptidase domain 3 isoform X1

See identical proteins and their annotated locations for XP_011240415.1

Conserved Domains (4) summary

smart00608
Location:289 → 420

ACR; ADAM Cysteine-Rich Domain

pfam00200
Location:210 → 285

Disintegrin

pfam01421
Location:1 → 188

Reprolysin; Reprolysin (M12B) family zinc metalloprotease

pfam01683
Location:406 → 455

EB; EB module
XM_017312524.2 → XP_017168013.1 A disintegrin and metallopeptidase domain 3 isoform X1

Conserved Domains (4) summary

smart00608
Location:289 → 420

ACR; ADAM Cysteine-Rich Domain

pfam00200
Location:210 → 285

Disintegrin

pfam01421
Location:1 → 188

Reprolysin; Reprolysin (M12B) family zinc metalloprotease

pfam01683
Location:406 → 455

EB; EB module
XM_011242114.3 → XP_011240416.1 A disintegrin and metallopeptidase domain 3 isoform X2

Conserved Domains (3) summary

pfam00200
Location:404 → 444

Disintegrin

pfam01421
Location:187 → 382

Reprolysin; Reprolysin (M12B) family zinc metalloprotease

pfam01562
Location:27 → 144

Pep_M12B_propep; Reprolysin family propeptide