Prss28 serine protease 28 [Mus musculus (house mouse)] - Gene

Summary

Official Symbol: Prss28provided by MGI
Official Full Name: serine protease 28provided by MGI
Primary source: MGI:MGI:2149951
See related: Ensembl:ENSMUSG00000024171 AllianceGenome:MGI:2149951
Gene type: protein coding
RefSeq status: VALIDATED
Organism: Mus musculus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
Also known as: Isp1; mIsp-1
Summary: Enables serine-type peptidase activity. Acts upstream of or within blastocyst hatching; embryo implantation; and proteolysis. Located in extracellular space. Is expressed in several structures, including early conceptus; reproductive system; and telencephalon. [provided by Alliance of Genome Resources, Apr 2022]
Expression: Low expression observed in reference dataset See more
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Genomic context

Location:: 17 A3.3; 17 12.53 cM

Exon count:: 6

Annotation release	Status	Assembly	Chr	Location
RS_2024_02	current	GRCm39 (GCF_000001635.27)	17	NC_000083.7 (25527620..25530850)
108.20200622	previous assembly	GRCm38.p6 (GCF_000001635.26)	17	NC_000083.6 (25308646..25311876)

Chromosome 17 - NC_000083.7 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Expression

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See details

Project title: Mouse ENCODE transcriptome data
Description: RNA profiling data sets generated by the Mouse ENCODE project.
BioProject: PRJNA66167
Publication: PMID 25409824
Analysis date: n/a

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Generation and analysis of Prss28 and Prss29 deficient mice using CRISPR-Cas9 genome-editing.
Title: Generation and analysis of Prss28 and Prss29 deficient mice using CRISPR-Cas9 genome-editing.
Hypothyroidism induces a significant reduction of proteases Isp1 and Isp2 gene expressions and Notch signaling.
Title: Thyroid hormone regulates protease expression and activation of Notch signaling in implantation and embryo development.
ISP1 might signal to cells by cleaving and activating proteinase-activated receptors (PARs) and therefore assessed PARs 1, 2 and 4 as potential ISP1 targets.
Title: Implantation serine proteinase 1 exhibits mixed substrate specificity that silences signaling via proteinase-activated receptors.
the ISP1 and ISP2 genes are co-expressed within the endometrial gland
Title: Embryonic hatching enzyme strypsin/ISP1 is expressed with ISP2 in endometrial glands during implantation.
organization, structure and postulated function of the implantation serine proteinase (ISP1 and 2) genes.
Title: Murine implantation serine proteinases 1 and 2: structure, function and evolution.
ISP1 plays a critical role in embryo hatching and implantation
Title: Implantation Serine Proteinases heterodimerize and are critical in hatching and implantation.

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Variation

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Alleles

Alleles of this type are documented at Mouse Genome Informatics (MGI)

Endonuclease-mediated (1) 1 citation

General gene information

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Markers

NoName (e-PCR)
- UniSTS:491540

Prss28 (e-PCR), detects polymorphism
- UniSTS:545867

Isp1 (e-PCR), detects polymorphism
- UniSTS:265720

Gene Ontology Provided by MGI

Function	Evidence Code	Pubs
enables hydrolase activity	IEA Inferred from Electronic Annotation more info
enables peptidase activity	IEA Inferred from Electronic Annotation more info
enables protein binding	IPI Inferred from Physical Interaction more info	PubMed
enables serine-type endopeptidase activity	IBA Inferred from Biological aspect of Ancestor more info
enables serine-type peptidase activity	IDA Inferred from Direct Assay more info	PubMed
enables serine-type peptidase activity	IMP Inferred from Mutant Phenotype more info	PubMed

Process	Evidence Code	Pubs
acts_upstream_of_or_within blastocyst hatching	IGI Inferred from Genetic Interaction more info	PubMed
acts_upstream_of_or_within blastocyst hatching	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within embryo implantation	IGI Inferred from Genetic Interaction more info	PubMed
acts_upstream_of_or_within embryo implantation	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in proteolysis	IBA Inferred from Biological aspect of Ancestor more info
acts_upstream_of_or_within proteolysis	IMP Inferred from Mutant Phenotype more info	PubMed

Component	Evidence Code	Pubs
located_in extracellular region	IDA Inferred from Direct Assay more info	PubMed
is_active_in extracellular space	IBA Inferred from Biological aspect of Ancestor more info
located_in extracellular space	IDA Inferred from Direct Assay more info	PubMed

General protein information

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Preferred Names: serine protease 28

Names: ISP-1; implantation serine protease 1; implantation serine proteinase 1; protease, serine 28; strypsin; tryptase-like proteinase

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_053259.2 → NP_444489.2 serine protease 28 precursor

See identical proteins and their annotated locations for NP_444489.2

Status: VALIDATED

Source sequence(s)

AC122454, AK144498

Consensus CDS

CCDS28515.1

UniProtKB/Swiss-Prot

Q3UN30, Q924N9

UniProtKB/TrEMBL

A0A0R4IZZ8

Related

ENSMUSP00000015267.5, ENSMUST00000015267.6

Conserved Domains (2) summary

smart00020
Location:31 → 269

Tryp_SPc; Trypsin-like serine protease

cd00190
Location:31 → 272

Tryp_SPc; Trypsin-like serine protease; Many of these are synthesized as inactive precursor zymogens that are cleaved during limited proteolysis to generate their active forms. Alignment contains also inactive enzymes that have substitutions of the catalytic triad ...