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Arhgef28 Rho guanine nucleotide exchange factor 28 [ Mus musculus (house mouse) ]

Gene ID: 110596, updated on 5-Mar-2024

Summary

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Official Symbol
Arhgef28provided by MGI
Official Full Name
Rho guanine nucleotide exchange factor 28provided by MGI
Primary source
MGI:MGI:1346016
See related
Ensembl:ENSMUSG00000021662 AllianceGenome:MGI:1346016
Gene type
protein coding
RefSeq status
VALIDATED
Organism
Mus musculus
Lineage
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
Also known as
RIP2; Rgnef; RhoGEF; Rhoip2; p190RhoGEF; D13Bwg1089e; 9230110L08Rik
Summary
Predicted to enable guanyl-nucleotide exchange factor activity. Acts upstream of or within central nervous system neuron axonogenesis and neurofilament cytoskeleton organization. Predicted to be located in cytoplasm and plasma membrane. Is expressed in adrenal gland; genitourinary system; oral epithelium; paravertebral ganglion; and stomach. Orthologous to human ARHGEF28 (Rho guanine nucleotide exchange factor 28). [provided by Alliance of Genome Resources, Apr 2022]
Expression
Broad expression in kidney adult (RPKM 5.2), ovary adult (RPKM 5.2) and 22 other tissues See more
Orthologs
human all
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Genomic context

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See Arhgef28 in Genome Data Viewer
Location:
13 D1; 13 51.6 cM
Exon count:
42
Annotation release Status Assembly Chr Location
RS_2024_02 current GRCm39 (GCF_000001635.27) 13 NC_000079.7 (98035105..98343183, complement)
108.20200622 previous assembly GRCm38.p6 (GCF_000001635.26) 13 NC_000079.6 (97898594..98206675, complement)

Chromosome 13 - NC_000079.7Genomic Context describing neighboring genes Neighboring gene predicted gene, 41031 Neighboring gene VISTA enhancer mm1001 Neighboring gene predicted gene, 34388 Neighboring gene STARR-seq mESC enhancer starr_35408 Neighboring gene STARR-positive B cell enhancer ABC_E7267 Neighboring gene STARR-seq mESC enhancer starr_35410 Neighboring gene STARR-positive B cell enhancer ABC_E11554 Neighboring gene predicted gene, 57601 Neighboring gene STARR-seq mESC enhancer starr_35412 Neighboring gene STARR-seq mESC enhancer starr_35415 Neighboring gene predicted gene, 34469 Neighboring gene STARR-positive B cell enhancer ABC_E5411 Neighboring gene UTP15 small subunit processome component Neighboring gene ankyrin repeat family A member 2

Genomic regions, transcripts, and products

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Go to nucleotide: Graphics FASTA GenBank

Expression

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  • Project title: Mouse ENCODE transcriptome data
  • Description: RNA profiling data sets generated by the Mouse ENCODE project.
  • BioProject: PRJNA66167
  • Publication: PMID 25409824
  • Analysis date: n/a

Bibliography

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Related articles in PubMed

  1. Rho guanine nucleotide exchange factor (RGNEF) is a prosurvival factor under stress conditions. Cheung K, et al. Mol Cell Neurosci, 2017 Jul. PMID 28495450
  2. Control of basement membrane remodeling and epithelial branching morphogenesis in embryonic lung by Rho and cytoskeletal tension. Moore KA, et al. Dev Dyn, 2005 Feb. PMID 15614768
  3. Rgnef promotes ovarian tumor progression and confers protection from oxidative stress. Kleinschmidt EG, et al. Oncogene, 2019 Sep. PMID 31308489, Free PMC Article
  4. Rgnef (p190RhoGEF) knockout inhibits RhoA activity, focal adhesion establishment, and cell motility downstream of integrins. Miller NL, et al. PLoS One, 2012. PMID 22649559, Free PMC Article
  5. A role of STAT3 in Rho GTPase-regulated cell migration and proliferation. Debidda M, et al. J Biol Chem, 2005 Apr 29. PMID 15705584

See all (37) citations in PubMed

GeneRIFs: Gene References Into Functions

What's a GeneRIF?
  1. Over-Expression of p190RhoGEF Regulates the Formation of Atherosclerotic Plaques in the Aorta of ApoE[-/-] Mice via Macrophage Polarization.
    Title: Over-Expression of p190RhoGEF Regulates the Formation of Atherosclerotic Plaques in the Aorta of ApoE(-/-) Mice via Macrophage Polarization.
  2. A new role for Rgnef in ovarian cancer to facilitate NF-kappaB-mediated gene expression protecting cells from oxidative stress.
    Title: Rgnef promotes ovarian tumor progression and confers protection from oxidative stress.
  3. observed that the expression of RGNEF is upregulated in murine spinal motor neurons following distal sciatic nerve injury
    Title: Rho guanine nucleotide exchange factor (RGNEF) is a prosurvival factor under stress conditions.
  4. Rgnef promotes integrin beta 1-stimulated focal adhesion kinase activation.
    Title: A non-canonical role for Rgnef in promoting integrin-stimulated focal adhesion kinase activation.
  5. An essential role for Rgnef in RhoA regulation downstream of integrins in control of cell migration.
    Title: Rgnef (p190RhoGEF) knockout inhibits RhoA activity, focal adhesion establishment, and cell motility downstream of integrins.
  6. changes in cytoskeletal tension mediated by Rho signaling through ROCK may play an important role in the establishment of the spatial differentials in cell growth and extracellular matrix remodeling that drive embryonic lung development
    Title: Control of basement membrane remodeling and epithelial branching morphogenesis in embryonic lung by Rho and cytoskeletal tension.
  7. STAT3 represents an essential effector pathway of Rho GTPases in regulating multiple cellular functions including actin cytoskeleton reorganization, cell migration, gene activation, and proliferation
    Title: A role of STAT3 in Rho GTPase-regulated cell migration and proliferation.
Submit: New GeneRIF Correction

Variation

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Alleles

Alleles of this type are documented at Mouse Genome Informatics  (MGI)
  • Targeted (4)  1 citation
  • Endonuclease-mediated (3) 

Pathways from PubChem

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Interactions

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Products Interactant Other Gene Complex Source Pubs Description

General gene information

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Markers

Homology

Gene Ontology Provided by MGI

Function Evidence Code Pubs
enables RNA binding IEA
Inferred from Electronic Annotation
more info
  
enables guanyl-nucleotide exchange factor activity ISO
Inferred from Sequence Orthology
more info
  
enables metal ion binding IEA
Inferred from Electronic Annotation
more info
  
enables protein binding IPI
Inferred from Physical Interaction
more info
 PubMed 
Process Evidence Code Pubs
acts_upstream_of_or_within cell differentiation IEA
Inferred from Electronic Annotation
more info
  
involved_in cell morphogenesis IBA
Inferred from Biological aspect of Ancestor
more info
  
acts_upstream_of_or_within central nervous system neuron axonogenesis IMP
Inferred from Mutant Phenotype
more info
 PubMed 
acts_upstream_of_or_within neurofilament cytoskeleton organization IMP
Inferred from Mutant Phenotype
more info
 PubMed 
involved_in regulation of Rho protein signal transduction IBA
Inferred from Biological aspect of Ancestor
more info
  
Component Evidence Code Pubs
located_in cytoplasm IEA
Inferred from Electronic Annotation
more info
  
located_in membrane IEA
Inferred from Electronic Annotation
more info
  
located_in plasma membrane IEA
Inferred from Electronic Annotation
more info
  

General protein information

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Preferred Names
rho guanine nucleotide exchange factor 28
Names
190 kDa guanine nucleotide exchange factor
Rho guanine nucleotide exchange factor (GEF) 28
Rho interacting protein 2
Rho specific exchange factor
Rho-guanine nucleotide exchange factor
p190-RhoGEF
rho-interacting protein 2

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

  1. NM_001408684.1 → NP_001395613.1  rho guanine nucleotide exchange factor 28 isoform 2

    Status: VALIDATED

    Source sequence(s)
    AC123056, AC124397, AC133507

  2. NM_001408685.1 → NP_001395614.1  rho guanine nucleotide exchange factor 28 isoform 3

    Status: VALIDATED

    Source sequence(s)
    AC123056, AC124397, AC133507

  3. NM_001408686.1 → NP_001395615.1  rho guanine nucleotide exchange factor 28 isoform 4

    Status: VALIDATED

    Source sequence(s)
    AC124397, AC133507
    UniProtKB/TrEMBL
    A0A571BEH9
    Related
    ENSMUSP00000158740.2, ENSMUST00000238746.2

  4. NM_001408687.1 → NP_001395616.1  rho guanine nucleotide exchange factor 28 isoform 5

    Status: VALIDATED

    Source sequence(s)
    AC123056, AC124397, AC133507

  5. NM_012026.3 → NP_036156.2  rho guanine nucleotide exchange factor 28 isoform 1

    See identical proteins and their annotated locations for NP_036156.2

    Status: VALIDATED

    Source sequence(s)
    AC123056, AC124397, AC133507
    Consensus CDS
    CCDS36758.1
    UniProtKB/Swiss-Prot
    A0PJC1, B2RQQ2, G5E8P2, P97433, Q69Z41, Q6DI55
    Related
    ENSMUSP00000105053.2, ENSMUST00000109426.3
    Conserved Domains (4) summary
    cd00029
    Location:652 → 697
    C1; Protein kinase C conserved region 1 (C1) . Cysteine-rich zinc binding domain. Some members of this domain family bind phorbol esters and diacylglycerol, some are reported to bind RasGTP. May occur in tandem arrangement. Diacylglycerol (DAG) is a second ...
    cd00160
    Location:847 → 1039
    RhoGEF; Guanine nucleotide exchange factor for Rho/Rac/Cdc42-like GTPases; Also called Dbl-homologous (DH) domain. It appears that PH domains invariably occur C-terminal to RhoGEF/DH domains.
    cd14680
    Location:1084 → 1184
    PH_p190RhoGEF; Rho guanine nucleotide exchange factor Pleckstrin homology domain
    pfam00169
    Location:1120 → 1185
    PH; PH domain

RNA

  1. NR_176793.1 RNA Sequence

    Status: VALIDATED

    Source sequence(s)
    AC123056, AC124397, AC133507
    Related
    ENSMUST00000225884.2

  2. NR_176794.1 RNA Sequence

    Status: VALIDATED

    Source sequence(s)
    AC123056, AC124397, AC133507

RefSeqs of Annotated Genomes: GCF_000001635.27-RS_2024_02

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCm39 C57BL/6J

Genomic

  1. NC_000079.7 Reference GRCm39 C57BL/6J

    Range
    98035105..98343183 complement
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. XM_006517522.4 → XP_006517585.1  rho guanine nucleotide exchange factor 28 isoform X6

    Conserved Domains (4) summary
    PRK00286
    Location:973 → 1129
    xseA; exodeoxyribonuclease VII large subunit; Reviewed
    cd00029
    Location:275 → 320
    C1; Protein kinase C conserved region 1 (C1) . Cysteine-rich zinc binding domain. Some members of this domain family bind phorbol esters and diacylglycerol, some are reported to bind RasGTP. May occur in tandem arrangement. Diacylglycerol (DAG) is a second ...
    cd00160
    Location:470 → 662
    RhoGEF; Guanine nucleotide exchange factor for Rho/Rac/Cdc42-like GTPases; Also called Dbl-homologous (DH) domain. It appears that PH domains invariably occur C-terminal to RhoGEF/DH domains.
    cl17171
    Location:707 → 807
    PH-like; Pleckstrin homology-like domain

  2. XM_017315362.3 → XP_017170851.2  rho guanine nucleotide exchange factor 28 isoform X4

    Conserved Domains (4) summary
    PRK00286
    Location:1020 → 1176
    xseA; exodeoxyribonuclease VII large subunit; Reviewed
    cd00160
    Location:517 → 709
    RhoGEF; Guanine nucleotide exchange factor for Rho/Rac/Cdc42-like GTPases; Also called Dbl-homologous (DH) domain. It appears that PH domains invariably occur C-terminal to RhoGEF/DH domains.
    cd14680
    Location:754 → 854
    PH_p190RhoGEF; Rho guanine nucleotide exchange factor Pleckstrin homology domain
    cl00040
    Location:316 → 375
    C1; protein kinase C conserved region 1 (C1 domain) superfamily

  3. XM_006517521.4 → XP_006517584.1  rho guanine nucleotide exchange factor 28 isoform X5

    UniProtKB/TrEMBL
    A0A286YDI4
    Conserved Domains (3) summary
    cd00029
    Location:666 → 711
    C1; Protein kinase C conserved region 1 (C1) . Cysteine-rich zinc binding domain. Some members of this domain family bind phorbol esters and diacylglycerol, some are reported to bind RasGTP. May occur in tandem arrangement. Diacylglycerol (DAG) is a second ...
    cd00160
    Location:861 → 1053
    RhoGEF; Guanine nucleotide exchange factor for Rho/Rac/Cdc42-like GTPases; Also called Dbl-homologous (DH) domain. It appears that PH domains invariably occur C-terminal to RhoGEF/DH domains.
    cd14680
    Location:1098 → 1198
    PH_p190RhoGEF; Rho guanine nucleotide exchange factor Pleckstrin homology domain

  4. XM_036157834.1 → XP_036013727.1  rho guanine nucleotide exchange factor 28 isoform X7

    UniProtKB/TrEMBL
    A0A286YDI4
    Conserved Domains (3) summary
    cd00160
    Location:861 → 1053
    RhoGEF; Guanine nucleotide exchange factor for Rho/Rac/Cdc42-like GTPases; Also called Dbl-homologous (DH) domain. It appears that PH domains invariably occur C-terminal to RhoGEF/DH domains.
    cd14680
    Location:1098 → 1198
    PH_p190RhoGEF; Rho guanine nucleotide exchange factor Pleckstrin homology domain
    cl00040
    Location:660 → 719
    C1; protein kinase C conserved region 1 (C1 domain) superfamily
Nucleotide Protein Strain
Heading Accession and Version
Protein Accession Links
GenPept Link UniProtKB Link
P97433.2 GenPept UniProtKB/Swiss-Prot:P97433

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