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Rap1gap Rap1 GTPase-activating protein [ Mus musculus (house mouse) ]

Gene ID: 110351, updated on 5-Mar-2024

Summary

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Official Symbol
Rap1gapprovided by MGI
Official Full Name
Rap1 GTPase-activating proteinprovided by MGI
Primary source
MGI:MGI:109338
See related
Ensembl:ENSMUSG00000041351 AllianceGenome:MGI:109338
Gene type
protein coding
RefSeq status
VALIDATED
Organism
Mus musculus
Lineage
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
Also known as
Gap; ARPP-90; Rap1ga1; Rap1GAP1; 1300019I11Rik; 2310004O14Rik
Summary
Predicted to enable GTPase activator activity; protein homodimerization activity; and small GTPase binding activity. Acts upstream of or within positive regulation of cell-cell adhesion and positive regulation of phagocytosis. Predicted to be located in several cellular components, including dendrite; early endosome; and neuronal cell body. Predicted to be active in cytoplasm and membrane. Is expressed in several structures, including metanephros; nervous system; olfactory epithelium; pancreas; and skeleton. Orthologous to human RAP1GAP (RAP1 GTPase activating protein). [provided by Alliance of Genome Resources, Apr 2022]
Expression
Broad expression in adrenal adult (RPKM 86.3), colon adult (RPKM 64.5) and 19 other tissues See more
Orthologs
human all
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Genomic context

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See Rap1gap in Genome Data Viewer
Location:
4 D3; 4 70.02 cM
Exon count:
28
Annotation release Status Assembly Chr Location
RS_2024_02 current GRCm39 (GCF_000001635.27) 4 NC_000070.7 (137391549..137457172)
108.20200622 previous assembly GRCm38.p6 (GCF_000001635.26) 4 NC_000070.6 (137664711..137729861)

Chromosome 4 - NC_000070.7Genomic Context describing neighboring genes Neighboring gene low density lipoprotein receptor class A domain containing 2 Neighboring gene CapStarr-seq enhancer MGSCv37_chr4:137149587-137149696 Neighboring gene CapStarr-seq enhancer MGSCv37_chr4:137150478-137150661 Neighboring gene STARR-seq mESC enhancer starr_11788 Neighboring gene ubiquitin specific peptidase 48 Neighboring gene predicted gene, 54263 Neighboring gene RAP1 GTPase activating protein, opposite strand Neighboring gene predicted gene, 52682 Neighboring gene alkaline phosphatase, liver/bone/kidney Neighboring gene STARR-seq mESC enhancer starr_11793 Neighboring gene STARR-positive B cell enhancer ABC_E4691 Neighboring gene STARR-seq mESC enhancer starr_11795 Neighboring gene predicted gene, 32079

Genomic regions, transcripts, and products

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Go to nucleotide: Graphics FASTA GenBank

Expression

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  • Project title: Mouse ENCODE transcriptome data
  • Description: RNA profiling data sets generated by the Mouse ENCODE project.
  • BioProject: PRJNA66167
  • Publication: PMID 25409824
  • Analysis date: n/a

Bibliography

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Related articles in PubMed

  1. Plexins are GTPase-activating proteins for Rap and are activated by induced dimerization. Wang Y, et al. Sci Signal, 2012 Jan 17. PMID 22253263, Free PMC Article
  2. Impaired fertility and spermiogenetic disorders with loss of cell adhesion in male mice expressing an interfering Rap1 mutant. Aivatiadou E, et al. Mol Biol Cell, 2007 Apr. PMID 17314400, Free PMC Article
  3. BCR/ABL and IL-3 activate Rap1 to stimulate the B-Raf/MEK/Erk and Akt signaling pathways and to regulate proliferation, apoptosis, and adhesion. Jin A, et al. Oncogene, 2006 Jul 20. PMID 16518411
  4. Rap1 couples cAMP signaling to a distinct pool of p42/44MAPK regulating excitability, synaptic plasticity, learning, and memory. Morozov A, et al. Neuron, 2003 Jul 17. PMID 12873387
  5. Phosphorylation of Rap1GAP, a striatally enriched protein, by protein kinase A controls Rap1 activity and dendritic spine morphology. McAvoy T, et al. Proc Natl Acad Sci U S A, 2009 Mar 3. PMID 19218462, Free PMC Article

See all (49) citations in PubMed

GeneRIFs: Gene References Into Functions

What's a GeneRIF?
  1. increased podocyte expression of RAP1GAP contributes directly to podocyte dysfunction by a mechanism that involves loss of RAP1-mediated activation of beta1 integrin
    Title: Podocyte-specific RAP1GAP expression contributes to focal segmental glomerulosclerosis-associated glomerular injury.
  2. Plexins are GTPase-activating proteins for Rap and are activated by induced dimerization
    Title: Plexins are GTPase-activating proteins for Rap and are activated by induced dimerization.
  3. Ca2+-dependent monomer and dimer formation switches CAPRI Protein between Ras GTPase-activating protein (GAP) and RapGAP activities
    Title: Ca2+-dependent monomer and dimer formation switches CAPRI Protein between Ras GTPase-activating protein (GAP) and RapGAP activities.
  4. phosphorylation of Rap1GAP by protein kinase A plays an important role in striatal neurons by modulating Rap1 actions
    Title: Phosphorylation of Rap1GAP, a striatally enriched protein, by protein kinase A controls Rap1 activity and dendritic spine morphology.
  5. The transport of the signaling complex containing the p75 receptor and Rap1 may play a role in the effect of MAG (myelin-associated glycoprotein).
    Title: Rap1 is involved in the signal transduction of myelin-associated glycoprotein.
  6. CD44 stimulation induces inside-out activation of CR3 through the GTPase Rap1
    Title: CD44-mediated phagocytosis induces inside-out activation of complement receptor-3 in murine macrophages.
  7. GTPase Rap1 exhibits an inverse pattern of expression to pERK in individual Ag-specific primed and tolerized T cells.
    Title: Inverse Rap1 and phospho-ERK expression discriminate the maintenance phase of tolerance and priming of antigen-specific CD4+ T cells in vitro and in vivo.
  8. These findings proved a crucial role of the cross-talk between two Ras-family GTPases M-Ras and Rap1, mediated by RA-GEF-2, in adhesion signaling.
    Title: The M-Ras-RA-GEF-2-Rap1 pathway mediates tumor necrosis factor-alpha dependent regulation of integrin activation in splenocytes.
  9. Rap1 stimulates signal transduction pathways that lead to cell proliferation and cell survival, and may play crucial roles in leukemogenesis by BCR/ABL.
    Title: BCR/ABL and IL-3 activate Rap1 to stimulate the B-Raf/MEK/Erk and Akt signaling pathways and to regulate proliferation, apoptosis, and adhesion.
  10. Ras plays a major role in ERK activation by cAMP, PACAP, and KCl, while Rap1 also mediates activation of a selective membrane-associated pool of ERKs.
    Title: The requirement of Ras and Rap1 for the activation of ERKs by cAMP, PACAP, and KCl in cerebellar granule cells.
Submit: New GeneRIF Correction See all GeneRIFs (23)

Variation

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Alleles

Alleles of this type are documented at Mouse Genome Informatics  (MGI)
  • Targeted (1) 
  • Endonuclease-mediated (3) 

Pathways from PubChem

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Interactions

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Products Interactant Other Gene Complex Source Pubs Description

General gene information

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Markers

Homology

Gene Ontology Provided by MGI

Function Evidence Code Pubs
enables GTPase activator activity IBA
Inferred from Biological aspect of Ancestor
more info
  
enables GTPase activator activity ISO
Inferred from Sequence Orthology
more info
  
enables GTPase regulator activity IEA
Inferred from Electronic Annotation
more info
  
enables protein binding IPI
Inferred from Physical Interaction
more info
 PubMed 
enables protein homodimerization activity ISO
Inferred from Sequence Orthology
more info
  
enables small GTPase binding ISO
Inferred from Sequence Orthology
more info
  
Process Evidence Code Pubs
acts_upstream_of cell-cell adhesion IMP
Inferred from Mutant Phenotype
more info
 PubMed 
involved_in cellular response to glial cell derived neurotrophic factor ISO
Inferred from Sequence Orthology
more info
  
acts_upstream_of establishment of localization in cell IMP
Inferred from Mutant Phenotype
more info
 PubMed 
involved_in negative regulation of microvillus assembly ISO
Inferred from Sequence Orthology
more info
  
involved_in negative regulation of neuron differentiation ISO
Inferred from Sequence Orthology
more info
  
involved_in negative regulation of thyroid gland epithelial cell proliferation ISO
Inferred from Sequence Orthology
more info
  
acts_upstream_of phagocytosis IMP
Inferred from Mutant Phenotype
more info
 PubMed 
involved_in positive regulation of GTPase activity ISO
Inferred from Sequence Orthology
more info
  
acts_upstream_of_or_within positive regulation of cell-cell adhesion IMP
Inferred from Mutant Phenotype
more info
 PubMed 
acts_upstream_of_or_within positive regulation of phagocytosis IMP
Inferred from Mutant Phenotype
more info
 PubMed 
involved_in regulation of GTPase activity ISO
Inferred from Sequence Orthology
more info
  
involved_in regulation of small GTPase mediated signal transduction IEA
Inferred from Electronic Annotation
more info
  
Component Evidence Code Pubs
located_in Golgi apparatus IEA
Inferred from Electronic Annotation
more info
  
located_in axon ISO
Inferred from Sequence Orthology
more info
  
is_active_in cytoplasm IBA
Inferred from Biological aspect of Ancestor
more info
  
located_in cytosol ISO
Inferred from Sequence Orthology
more info
  
located_in dendrite ISO
Inferred from Sequence Orthology
more info
  
located_in early endosome ISO
Inferred from Sequence Orthology
more info
  
is_active_in membrane IBA
Inferred from Biological aspect of Ancestor
more info
  
located_in membrane ISO
Inferred from Sequence Orthology
more info
  
located_in neuronal cell body ISO
Inferred from Sequence Orthology
more info
  

General protein information

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Preferred Names
rap1 GTPase-activating protein 1

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

  1. NM_001081155.4 → NP_001074624.1  rap1 GTPase-activating protein 1 isoform 1

    Status: VALIDATED

    Description
    Transcript Variant: This variant (1) represents the longest transcript and encodes isoform 1.
    Source sequence(s)
    AL805954
    Consensus CDS
    CCDS38925.1
    UniProtKB/Swiss-Prot
    A2ALS5
    Related
    ENSMUSP00000042473.6, ENSMUST00000047243.12
    Conserved Domains (2) summary
    smart00390
    Location:61 → 81
    GoLoco; LGN motif, putative GEFs specific for G-alpha GTPases
    pfam02145
    Location:275 → 454
    Rap_GAP; Rap/ran-GAP

  2. NM_001256218.2 → NP_001243147.1  rap1 GTPase-activating protein 1 isoform 2

    Status: VALIDATED

    Description
    Transcript Variant: This variant (2) uses two different splice sites in the 3' coding region which shifts the reading frame, compared to variant 1. The resulting protein (isoform 2) has a longer and distinct C-terminus when it is compared to isoform 1.
    Source sequence(s)
    AL805954
    Consensus CDS
    CCDS57306.1
    UniProtKB/TrEMBL
    A2ALS4
    Related
    ENSMUSP00000101461.2, ENSMUST00000105835.8
    Conserved Domains (2) summary
    smart00390
    Location:61 → 81
    GoLoco; LGN motif, putative GEFs specific for G-alpha GTPases
    pfam02145
    Location:275 → 454
    Rap_GAP; Rap/ran-GAP

  3. NM_001331215.2 → NP_001318144.1  rap1 GTPase-activating protein 1 isoform 4

    Status: VALIDATED

    Description
    Transcript Variant: This variant (5) represents use of an alternate promoter and differs in the 5' UTR and 5' coding region compared to variant 1. The encoded isoform (4) has a shorter, distinct N-terminus compared to isoform 1.
    Source sequence(s)
    AL805954
    Consensus CDS
    CCDS84809.1
    UniProtKB/Swiss-Prot
    A2ALS5
    Related
    ENSMUSP00000095448.5, ENSMUST00000097837.11

  4. NM_001379233.1 → NP_001366162.1  rap1 GTPase-activating protein 1 isoform 5

    Status: VALIDATED

    Source sequence(s)
    AL805954
    Conserved Domains (2) summary
    smart00390
    Location:61 → 81
    GoLoco; LGN motif, putative GEFs specific for G-alpha GTPases
    pfam02145
    Location:275 → 454
    Rap_GAP; Rap/ran-GAP

  5. NM_001379234.1 → NP_001366163.1  rap1 GTPase-activating protein 1 isoform 6

    Status: VALIDATED

    Source sequence(s)
    AL805954
    Conserved Domains (2) summary
    smart00390
    Location:28 → 48
    GoLoco; LGN motif, putative GEFs specific for G-alpha GTPases
    pfam02145
    Location:242 → 421
    Rap_GAP; Rap/ran-GAP

  6. NM_001379235.1 → NP_001366164.1  rap1 GTPase-activating protein 1 isoform 7

    Status: VALIDATED

    Source sequence(s)
    AL805954
    Conserved Domains (2) summary
    smart00390
    Location:49 → 69
    GoLoco; LGN motif, putative GEFs specific for G-alpha GTPases
    pfam02145
    Location:263 → 442
    Rap_GAP; Rap/ran-GAP

  7. NM_001379236.1 → NP_001366165.1  rap1 GTPase-activating protein 1 isoform 8

    Status: VALIDATED

    Source sequence(s)
    AL805954
    Conserved Domains (2) summary
    smart00390
    Location:1 → 17
    GoLoco; LGN motif, putative GEFs specific for G-alpha GTPases
    pfam02145
    Location:211 → 390
    Rap_GAP; Rap/ran-GAP

  8. NM_029563.3 → NP_083839.1  rap1 GTPase-activating protein 1 isoform 3

    See identical proteins and their annotated locations for NP_083839.1

    Status: VALIDATED

    Description
    Transcript Variant: This variant (3) represents use of an alternate promoter and initiates translation at a downstream start codon, compared to variant 1. The encoded protein (isoform 3) is shorter when it is compared to isoform 1.
    Source sequence(s)
    AL805954
    UniProtKB/Swiss-Prot
    A2ALS5, E9PZZ8, Q6A063, Q80VZ8, Q8K2L6
    Conserved Domains (2) summary
    pfam02145
    Location:211 → 390
    Rap_GAP; Rap/ran-GAP
    pfam02188
    Location:1 → 17
    GoLoco; GoLoco motif

RNA

  1. NR_138591.1 RNA Sequence

    Status: VALIDATED

    Description
    Transcript Variant: This variant (4) represents use of an alternate promoter and uses an alternate splice site compared to variant 1. This variant is represented as non-coding because the use of the 5'-most expected translational start codon renders the transcript a candidate for nonsense-mediated mRNA decay (NMD).
    Source sequence(s)
    AL805954

  2. NR_138592.1 RNA Sequence

    Status: VALIDATED

    Description
    Transcript Variant: This variant (6) represents use of an alternate promoter, differs in the 5' region, and uses an alternate splice site in the 3' region, compared to variant 1. This variant is represented as non-coding because the use of the 5'-most expected translational start codon renders the transcript a candidate for nonsense-mediated mRNA decay (NMD).
    Source sequence(s)
    AK005063, AK019076

RefSeqs of Annotated Genomes: GCF_000001635.27-RS_2024_02

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCm39 C57BL/6J

Genomic

  1. NC_000070.7 Reference GRCm39 C57BL/6J

    Range
    137391549..137457172
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. XM_006538482.5 → XP_006538545.1  rap1 GTPase-activating protein 1 isoform X8

    See identical proteins and their annotated locations for XP_006538545.1

    Conserved Domains (2) summary
    pfam02145
    Location:211 → 390
    Rap_GAP; Rap/ran-GAP
    pfam02188
    Location:1 → 17
    GoLoco; GoLoco motif

  2. XM_006538483.5 → XP_006538546.1  rap1 GTPase-activating protein 1 isoform X8

    See identical proteins and their annotated locations for XP_006538546.1

    Conserved Domains (2) summary
    pfam02145
    Location:211 → 390
    Rap_GAP; Rap/ran-GAP
    pfam02188
    Location:1 → 17
    GoLoco; GoLoco motif

  3. XM_030253087.2 → XP_030108947.1  rap1 GTPase-activating protein 1 isoform X9

    Conserved Domains (2) summary
    pfam02145
    Location:211 → 390
    Rap_GAP; Rap/ran-GAP
    pfam02188
    Location:1 → 17
    GoLoco; GoLoco motif

  4. XM_030253084.1 → XP_030108944.1  rap1 GTPase-activating protein 1 isoform X6

    Conserved Domains (2) summary
    pfam02145
    Location:263 → 442
    Rap_GAP; Rap/ran-GAP
    pfam02188
    Location:49 → 69
    GoLoco; GoLoco motif

  5. XM_006538475.5 → XP_006538538.1  rap1 GTPase-activating protein 1 isoform X1

    Conserved Domains (2) summary
    pfam02145
    Location:275 → 454
    Rap_GAP; Rap/ran-GAP
    pfam02188
    Location:61 → 81
    GoLoco; GoLoco motif

  6. XM_006538478.4 → XP_006538541.1  rap1 GTPase-activating protein 1 isoform X3

    Conserved Domains (2) summary
    pfam02145
    Location:263 → 442
    Rap_GAP; Rap/ran-GAP
    pfam02188
    Location:49 → 69
    GoLoco; GoLoco motif

  7. XM_036163582.1 → XP_036019475.1  rap1 GTPase-activating protein 1 isoform X2

    Conserved Domains (2) summary
    smart00390
    Location:50 → 70
    GoLoco; LGN motif, putative GEFs specific for G-alpha GTPases
    pfam02145
    Location:264 → 443
    Rap_GAP; Rap/ran-GAP

  8. XM_036163583.1 → XP_036019476.1  rap1 GTPase-activating protein 1 isoform X4

    Conserved Domains (2) summary
    smart00390
    Location:38 → 58
    GoLoco; LGN motif, putative GEFs specific for G-alpha GTPases
    pfam02145
    Location:252 → 431
    Rap_GAP; Rap/ran-GAP

  9. XM_036163584.1 → XP_036019477.1  rap1 GTPase-activating protein 1 isoform X7

    Conserved Domains (2) summary
    smart00390
    Location:38 → 58
    GoLoco; LGN motif, putative GEFs specific for G-alpha GTPases
    pfam02145
    Location:252 → 431
    Rap_GAP; Rap/ran-GAP

  10. XM_006538480.2 → XP_006538543.1  rap1 GTPase-activating protein 1 isoform X5

    Conserved Domains (2) summary
    pfam02145
    Location:242 → 421
    Rap_GAP; Rap/ran-GAP
    pfam02188
    Location:28 → 48
    GoLoco; GoLoco motif

RNA

  1. XR_003954867.2 RNA Sequence

  2. XR_003954870.2 RNA Sequence

  3. XR_003954866.2 RNA Sequence

  4. XR_003954868.2 RNA Sequence

  5. XR_004941798.1 RNA Sequence

  6. XR_004941796.1 RNA Sequence

  7. XR_004941797.1 RNA Sequence

  8. XR_390722.2 RNA Sequence

  9. XR_390723.4 RNA Sequence

  10. XR_001784080.2 RNA Sequence

  11. XR_001784081.3 RNA Sequence

  12. XR_004941795.1 RNA Sequence

  13. XR_003954869.2 RNA Sequence

Nucleotide Protein Strain
Heading Accession and Version
Protein Accession Links
GenPept Link UniProtKB Link
A2ALS5.2 GenPept UniProtKB/Swiss-Prot:A2ALS5

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