U.S. flag

An official website of the United States government

Format

Send to:

Choose Destination

HCST hematopoietic cell signal transducer [ Homo sapiens (human) ]

Gene ID: 10870, updated on 11-Apr-2024

Summary

Go to the top of the page Help
Official Symbol
HCSTprovided by HGNC
Official Full Name
hematopoietic cell signal transducerprovided by HGNC
Primary source
HGNC:HGNC:16977
See related
Ensembl:ENSG00000126264 MIM:604089; AllianceGenome:HGNC:16977
Gene type
protein coding
RefSeq status
REVIEWED
Organism
Homo sapiens
Lineage
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo
Also known as
DAP10; KAP10; PIK3AP
Summary
This gene encodes a transmembrane signaling adaptor that contains a YxxM motif in its cytoplasmic domain. The encoded protein may form part of the immune recognition receptor complex with the C-type lectin-like receptor NKG2D. As part of this receptor complex, this protein may activate phosphatidylinositol 3-kinase dependent signaling pathways through its intracytoplasmic YxxM motif. This receptor complex may have a role in cell survival and proliferation by activation of NK and T cell responses. Alternative splicing results in two transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008]
Expression
Biased expression in bone marrow (RPKM 39.5), spleen (RPKM 20.3) and 12 other tissues See more
Orthologs
mouse all
NEW
Try the new Gene table
Try the new Transcript table

Genomic context

Go to the top of the page Help
Location:
19q13.12
Exon count:
4
Annotation release Status Assembly Chr Location
RS_2023_10 current GRCh38.p14 (GCF_000001405.40) 19 NC_000019.10 (35902529..35904377)
RS_2023_10 current T2T-CHM13v2.0 (GCF_009914755.1) 19 NC_060943.1 (38448294..38450142)
105.20220307 previous assembly GRCh37.p13 (GCF_000001405.25) 19 NC_000019.9 (36393431..36395279)

Chromosome 19 - NC_000019.10Genomic Context describing neighboring genes Neighboring gene uncharacterized LOC124904705 Neighboring gene RNA, 7SL, cytoplasmic 402, pseudogene Neighboring gene NFKB inhibitor delta Neighboring gene H3K27ac-H3K4me1 hESC enhancer GRCh37_chr19:36389157-36389729 Neighboring gene H3K27ac-H3K4me1 hESC enhancer GRCh37_chr19:36389730-36390301 Neighboring gene H3K27ac hESC enhancers GRCh37_chr19:36390314-36390870 and GRCh37_chr19:36390871-36391426 Neighboring gene ReSE screen-validated silencer GRCh37_chr19:36398209-36398362 Neighboring gene ATAC-STARR-seq lymphoblastoid active region 14505 Neighboring gene ATAC-STARR-seq lymphoblastoid active region 14506 Neighboring gene ATAC-STARR-seq lymphoblastoid active region 14507 Neighboring gene transmembrane immune signaling adaptor TYROBP Neighboring gene ATAC-STARR-seq lymphoblastoid active region 14508 Neighboring gene ATAC-STARR-seq lymphoblastoid active region 14509 Neighboring gene leucine rich repeat and fibronectin type III domain containing 3

Genomic regions, transcripts, and products

Go to the top of the page Help

Go to nucleotide: Graphics FASTA GenBank

Expression

Go to the top of the page Help
  • Project title: HPA RNA-seq normal tissues
  • Description: RNA-seq was performed of tissue samples from 95 human individuals representing 27 different tissues in order to determine tissue-specificity of all protein-coding genes
  • BioProject: PRJEB4337
  • Publication: PMID 24309898
  • Analysis date: Wed Apr 4 07:08:55 2018

Bibliography

Go to the top of the pageHelp

Related articles in PubMed

  1. Comparative analysis of human NK cell activation induced by NKG2D and natural cytotoxicity receptors. André P, et al. Eur J Immunol, 2004 Apr. PMID 15048706
  2. DAP10 and DAP12 form distinct, but functionally cooperative, receptor complexes in natural killer cells. Wu J, et al. J Exp Med, 2000 Oct 2. PMID 11015446, Free PMC Article
  3. CSF1R and HCST: Novel Candidate Biomarkers Predicting the Response to Immunotherapy in Non-Small Cell Lung Cancer. Qi X, et al. Technol Cancer Res Treat, 2020 Jan-Dec. PMID 33153411, Free PMC Article
  4. Ubiquitin-dependent endocytosis of NKG2D-DAP10 receptor complexes activates signaling and functions in human NK cells. Quatrini L, et al. Sci Signal, 2015 Oct 27. PMID 26508790
  5. DNAX-activating protein 10 (DAP10) membrane adaptor associates with receptor for advanced glycation end products (RAGE) and modulates the RAGE-triggered signaling pathway in human keratinocytes. Sakaguchi M, et al. J Biol Chem, 2014 Aug 22. PMID 25002577, Free PMC Article

See all (32) citations in PubMed

GeneRIFs: Gene References Into Functions

What's a GeneRIF?
  1. CSF1R and HCST: Novel Candidate Biomarkers Predicting the Response to Immunotherapy in Non-Small Cell Lung Cancer.
    Title: CSF1R and HCST: Novel Candidate Biomarkers Predicting the Response to Immunotherapy in Non-Small Cell Lung Cancer.
  2. NKG2D-DAP10 signaling recruits EVL to the cytotoxic synapse to generate F-actin and promote NK cell cytotoxicity.
    Title: NKG2D-DAP10 signaling recruits EVL to the cytotoxic synapse to generate F-actin and promote NK cell cytotoxicity.
  3. These results suggest that NKG2D-DAP10 endocytosis represents a means to decrease cell surface receptor abundance, as well as to control signaling outcome in cytotoxic lymphocytes.
    Title: Ubiquitin-dependent endocytosis of NKG2D-DAP10 receptor complexes activates signaling and functions in human NK cells.
  4. findings indicate that the functional interaction between RAGE and DAP10 coordinately regulates S100A8/A9-mediated survival and/or apoptotic response of keratinocytes
    Title: DNAX-activating protein 10 (DAP10) membrane adaptor associates with receptor for advanced glycation end products (RAGE) and modulates the RAGE-triggered signaling pathway in human keratinocytes.
  5. TGF-beta1 may reduce the expression of NKG2D/DAP10 and 2B4/SAPin patients with hepatitis B.
    Title: TGF-β1 down-regulation of NKG2D/DAP10 and 2B4/SAP expression on human NK cells contributes to HBV persistence.
  6. HMBOX1 negatively regulates the expression of NKG2D and the activation of the NKG2D/DAP10 signaling pathway in NK cells.
    Title: HMBOX1 negatively regulates NK cell functions by suppressing the NKG2D/DAP10 signaling pathway.
  7. Other gamma(c) cytokines act similarly to IL-2 in up-regulating DAP10 expression and NKG2D-DAP10 surface expression.
    Title: Complex regulation of human NKG2D-DAP10 cell surface expression: opposing roles of the γc cytokines and TGF-β1.
  8. TGF-BETA1 down-modulates surface NKG2D expression by controlling the transcriptional and translational levels of DAP10.
    Title: A possible mechanism of impaired NK cytotoxicity in cancer patients: down-regulation of DAP10 by TGF-beta1.
  9. A transgenic mouse model demonstrates that induction of tolerance in Ly49H-positive natural killer (NK) cells by chronic exposure to virus-encoded ligand m157 requires signaling through the Ly49H adaptor protein DAP12, not the DAP10 adaptor protein.
    Title: Activation receptor-induced tolerance of mature NK cells in vivo requires signaling through the receptor and is reversible.
  10. the rapid degradation of NKG2D/DAP10 observed coincident with recruitment of the receptor to the cytotoxic immune synapse may explain the loss of NKG2D receptor expression after chronic exposure to NKG2D ligands
    Title: The traffic of the NKG2D/Dap10 receptor complex during natural killer (NK) cell activation.
Submit: New GeneRIF Correction See all GeneRIFs (13)

Phenotypes

Go to the top of the page Help

Find tests for this gene in the NIH Genetic Testing Registry (GTR)

Review eQTL and phenotype association data in this region using PheGenI

Variation

Go to the top of the page Help

See variants in ClinVar

See studies and variants in dbVar

See Variation Viewer (GRCh37.p13)

See Variation Viewer (GRCh38)

Genotypes

HIV-1 interactions

Go to the top of the page Help

Replication interactions

Interaction Pubs
HIV-1 infection (VSV-G pseudotyped) of CEMT4 T cells downregulates plasma membrane expression of HCST PubMed

Go to the HIV-1, Human Interaction Database

Pathways from PubChem

Go to the top of the page

Interactions

Go to the top of the page Help
Products Interactant Other Gene Complex Source Pubs Description

General gene information

Go to the top of the page Help

Markers

Homology

Clone Names

  • DKFZP586C1522

Gene Ontology Provided by GOA

Function Evidence Code Pubs
enables phosphatidylinositol 3-kinase binding IBA
Inferred from Biological aspect of Ancestor
more info
  
enables phosphatidylinositol 3-kinase binding IDA
Inferred from Direct Assay
more info
 PubMed 
enables protein binding IPI
Inferred from Physical Interaction
more info
 PubMed 
enables signaling receptor binding IBA
Inferred from Biological aspect of Ancestor
more info
  
enables signaling receptor binding IPI
Inferred from Physical Interaction
more info
 PubMed 
Process Evidence Code Pubs
involved_in positive regulation of phosphatidylinositol 3-kinase/protein kinase B signal transduction IBA
Inferred from Biological aspect of Ancestor
more info
  
involved_in positive regulation of phosphatidylinositol 3-kinase/protein kinase B signal transduction IDA
Inferred from Direct Assay
more info
 PubMed 
involved_in protein phosphorylation IGI
Inferred from Genetic Interaction
more info
 PubMed 
involved_in regulation of immune response IEA
Inferred from Electronic Annotation
more info
  
Component Evidence Code Pubs
located_in cell surface IDA
Inferred from Direct Assay
more info
 PubMed 
located_in plasma membrane TAS
Traceable Author Statement
more info
  

General protein information

Go to the top of the page Help
Preferred Names
hematopoietic cell signal transducer
Names
DNAX-activation protein 10
kinase assoc pro of ~10kDa
kinase assoc protein
membrane protein DAP10
phosphoinositide-3-kinase adaptor protein
transmembrane adapter protein KAP10

NCBI Reference Sequences (RefSeq)

Go to the top of the page Help

NEW Try the new Transcript table

RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

  1. NM_001007469.2 → NP_001007470.1  hematopoietic cell signal transducer isoform 2 precursor

    See identical proteins and their annotated locations for NP_001007470.1

    Status: REVIEWED

    Description
    Transcript Variant: This variant (2) uses an alternate in-frame splice site in the 3' coding region, compared to variant 1, resulting in a shorter protein (isoform 2).
    Source sequence(s)
    AF072844, AL050163
    Consensus CDS
    CCDS46057.1
    UniProtKB/Swiss-Prot
    Q9UBK5
    Related
    ENSP00000400516.1, ENST00000437550.2
    Conserved Domains (1) summary
    pfam07213
    Location:1 → 92
    DAP10; DAP10 membrane protein

  2. NM_014266.4 → NP_055081.1  hematopoietic cell signal transducer isoform 1 precursor

    See identical proteins and their annotated locations for NP_055081.1

    Status: REVIEWED

    Description
    Transcript Variant: This variant (1) encodes the longer isoform (1).
    Source sequence(s)
    AD000864, AL050163
    Consensus CDS
    CCDS32998.1
    UniProtKB/Swiss-Prot
    Q9UBK5, Q9UBS1, Q9Y3Y0
    Related
    ENSP00000246551.3, ENST00000246551.9
    Conserved Domains (1) summary
    pfam07213
    Location:1 → 93
    DAP10; DAP10 membrane protein

RefSeqs of Annotated Genomes: GCF_000001405.40-RS_2023_10

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCh38.p14 Primary Assembly

Genomic

  1. NC_000019.10 Reference GRCh38.p14 Primary Assembly

    Range
    35902529..35904377
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. XM_017026193.2 → XP_016881682.1  hematopoietic cell signal transducer isoform X1

  2. XM_047438090.1 → XP_047294046.1  hematopoietic cell signal transducer isoform X2

Alternate T2T-CHM13v2.0

Genomic

  1. NC_060943.1 Alternate T2T-CHM13v2.0

    Range
    38448294..38450142
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. XM_054319594.1 → XP_054175569.1  hematopoietic cell signal transducer isoform X1

  2. XM_054319595.1 → XP_054175570.1  hematopoietic cell signal transducer isoform X2

Nucleotide Protein
Heading Accession and Version
Protein Accession Links
GenPept Link UniProtKB Link
Q9UBK5.1 GenPept UniProtKB/Swiss-Prot:Q9UBK5

Gene LinkOut

The following LinkOut resources are supplied by external providers. These providers are responsible for maintaining the links.

Chemical Information
Molecular Biology Databases
Research Materials
Tools
Miscellaneous