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Mthfs 5, 10-methenyltetrahydrofolate synthetase [ Mus musculus (house mouse) ]

Gene ID: 107885, updated on 5-Mar-2024

Summary

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Official Symbol
Mthfsprovided by MGI
Official Full Name
5, 10-methenyltetrahydrofolate synthetaseprovided by MGI
Primary source
MGI:MGI:1340032
See related
Ensembl:ENSMUSG00000066442 AllianceGenome:MGI:1340032
Gene type
protein coding
RefSeq status
VALIDATED
Organism
Mus musculus
Lineage
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
Also known as
1110034I12Rik; 2310020H23Rik
Summary
Predicted to enable 5-formyltetrahydrofolate cyclo-ligase activity; anion binding activity; and double-stranded DNA binding activity. Predicted to be involved in DNA geometric change; folic acid-containing compound metabolic process; and termination of mitochondrial transcription. Located in mitochondrion. Is expressed in several structures, including alimentary system; genitourinary system; integumental system; nervous system; and sensory organ. Orthologous to several human genes including ST20-MTHFS (ST20-MTHFS readthrough). [provided by Alliance of Genome Resources, Apr 2022]
Expression
Ubiquitous expression in liver E18 (RPKM 15.5), liver adult (RPKM 15.0) and 28 other tissues See more
Orthologs
human all
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Genomic context

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Location:
9 E3.1; 9 47.24 cM
Exon count:
4
Annotation release Status Assembly Chr Location
RS_2024_02 current GRCm39 (GCF_000001635.27) 9 NC_000075.7 (89093243..89122278)
108.20200622 previous assembly GRCm38.p6 (GCF_000001635.26) 9 NC_000075.6 (89211190..89240225)

Chromosome 9 - NC_000075.7Genomic Context describing neighboring genes Neighboring gene STARR-positive B cell enhancer mm9_chr9:89090619-89090919 Neighboring gene STARR-positive B cell enhancer ABC_E6785 Neighboring gene predicted gene, 40544 Neighboring gene STARR-positive B cell enhancer ABC_E8305 Neighboring gene STARR-positive B cell enhancer ABC_E11418 Neighboring gene B cell leukemia/lymphoma 2 related protein A1b Neighboring gene STARR-seq mESC enhancer starr_24872 Neighboring gene STARR-seq mESC enhancer starr_24873 Neighboring gene predicted gene, 40543 Neighboring gene STARR-seq mESC enhancer starr_24874 Neighboring gene STARR-seq mESC enhancer starr_24875 Neighboring gene STARR-seq mESC enhancer starr_24876 Neighboring gene predicted gene, 57551

Genomic regions, transcripts, and products

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Go to nucleotide: Graphics FASTA GenBank

Expression

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  • Project title: Mouse ENCODE transcriptome data
  • Description: RNA profiling data sets generated by the Mouse ENCODE project.
  • BioProject: PRJNA66167
  • Publication: PMID 25409824
  • Analysis date: n/a

Bibliography

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Related articles in PubMed

  1. 5,10-Methenyltetrahydrofolate synthetase activity is increased in tumors and modifies the efficacy of antipurine LY309887. Field MS, et al. Arch Biochem Biophys, 2009 Jan 15. PMID 19022216, Free PMC Article
  2. Inhibition of 5,10-methenyltetrahydrofolate synthetase. Field MS, et al. Arch Biochem Biophys, 2007 Feb 15. PMID 17250800, Free PMC Article
  3. Methenyltetrahydrofolate synthetase is a high-affinity catecholamine-binding protein. Anguera MC, et al. Arch Biochem Biophys, 2006 Nov 15. PMID 17055997, Free PMC Article
  4. Regulation of de novo purine biosynthesis by methenyltetrahydrofolate synthetase in neuroblastoma. Field MS, et al. J Biol Chem, 2006 Feb 17. PMID 16365037
  5. Mthfs is an Essential Gene in Mice and a Component of the Purinosome. Field MS, et al. Front Genet, 2011. PMID 22303332, Free PMC Article

See all (24) citations in PubMed

GeneRIFs: Gene References Into Functions

What's a GeneRIF?
  1. Expression of the MTHFS cDNA in MCF-7 cells increased the concentration of NADA required to deplete cellular folate.
    Title: Methenyltetrahydrofolate synthetase is a high-affinity catecholamine-binding protein.
Submit: New GeneRIF Correction

Variation

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Alleles

Alleles of this type are documented at Mouse Genome Informatics  (MGI)
  • Gene trapped (1) 
  • Endonuclease-mediated (1) 

Pathways from PubChem

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Interactions

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Products Interactant Other Gene Complex Source Pubs Description

General gene information

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Markers

Homology

Gene Ontology Provided by MGI

Function Evidence Code Pubs
enables 5-formyltetrahydrofolate cyclo-ligase activity IBA
Inferred from Biological aspect of Ancestor
more info
  
enables 5-formyltetrahydrofolate cyclo-ligase activity ISO
Inferred from Sequence Orthology
more info
  
enables ATP binding ISO
Inferred from Sequence Orthology
more info
  
enables double-stranded DNA binding ISO
Inferred from Sequence Orthology
more info
  
enables folic acid binding ISO
Inferred from Sequence Orthology
more info
  
enables ligase activity IEA
Inferred from Electronic Annotation
more info
  
enables metal ion binding IEA
Inferred from Electronic Annotation
more info
  
enables nucleotide binding IEA
Inferred from Electronic Annotation
more info
  
Process Evidence Code Pubs
involved_in DNA geometric change ISO
Inferred from Sequence Orthology
more info
  
involved_in DNA-templated transcription termination ISO
Inferred from Sequence Orthology
more info
  
involved_in folic acid catabolic process ISO
Inferred from Sequence Orthology
more info
  
involved_in folic acid-containing compound biosynthetic process IBA
Inferred from Biological aspect of Ancestor
more info
  
involved_in glutamate metabolic process ISO
Inferred from Sequence Orthology
more info
  
involved_in termination of mitochondrial transcription ISO
Inferred from Sequence Orthology
more info
  
involved_in tetrahydrofolate interconversion IBA
Inferred from Biological aspect of Ancestor
more info
  
involved_in tetrahydrofolate interconversion ISO
Inferred from Sequence Orthology
more info
  
involved_in tetrahydrofolate metabolic process ISO
Inferred from Sequence Orthology
more info
  
Component Evidence Code Pubs
located_in cytoplasm ISO
Inferred from Sequence Orthology
more info
  
located_in cytosol ISO
Inferred from Sequence Orthology
more info
  
located_in mitochondrial matrix ISO
Inferred from Sequence Orthology
more info
  
located_in mitochondrial nucleoid ISO
Inferred from Sequence Orthology
more info
  
located_in mitochondrion HDA PubMed 
is_active_in mitochondrion IBA
Inferred from Biological aspect of Ancestor
more info
  
located_in mitochondrion ISO
Inferred from Sequence Orthology
more info
  

General protein information

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Preferred Names
5-formyltetrahydrofolate cyclo-ligase
Names
5,10-methenyl-tetrahydrofolate synthetase
methenyl-THF synthetase
NP_001346922.1
NP_081105.1

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

  1. NM_001359993.1 → NP_001346922.1  5-formyltetrahydrofolate cyclo-ligase isoform 2

    Status: VALIDATED

    Source sequence(s)
    AC135634, CT030686
    Conserved Domains (1) summary
    pfam01812
    Location:1 → 141
    5-FTHF_cyc-lig; 5-formyltetrahydrofolate cyclo-ligase family

  2. NM_026829.2 → NP_081105.1  5-formyltetrahydrofolate cyclo-ligase isoform 1

    See identical proteins and their annotated locations for NP_081105.1

    Status: VALIDATED

    Source sequence(s)
    AK004092
    Consensus CDS
    CCDS23394.1
    UniProtKB/Swiss-Prot
    B2KF82, Q9D110
    UniProtKB/TrEMBL
    Q3TYX5
    Related
    ENSMUSP00000082354.7, ENSMUST00000085256.8
    Conserved Domains (1) summary
    pfam01812
    Location:10 → 198
    5-FTHF_cyc-lig; 5-formyltetrahydrofolate cyclo-ligase family

RefSeqs of Annotated Genomes: GCF_000001635.27-RS_2024_02

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCm39 C57BL/6J

Genomic

  1. NC_000075.7 Reference GRCm39 C57BL/6J

    Range
    89093243..89122278
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)
Nucleotide Protein Strain
Heading Accession and Version
Protein Accession Links
GenPept Link UniProtKB Link
Q9D110.2 GenPept UniProtKB/Swiss-Prot:Q9D110

Gene LinkOut

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