Me2 malic enzyme 2, NAD(+)-dependent, mitochondrial [Mus musculus (house mouse)] - Gene

Summary

Official Symbol: Me2provided by MGI
Official Full Name: malic enzyme 2, NAD(+)-dependent, mitochondrialprovided by MGI
Primary source: MGI:MGI:2147351
See related: Ensembl:ENSMUSG00000024556 AllianceGenome:MGI:2147351
Gene type: protein coding
RefSeq status: VALIDATED
Organism: Mus musculus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
Also known as: NAD-ME; D030040L20Rik
Summary: Predicted to enable malate dehydrogenase (decarboxylating) (NADP+) activity. Predicted to be involved in malate metabolic process; pyruvate metabolic process; and regulation of NADP metabolic process. Located in mitochondrion. Is expressed in several structures, including central nervous system; integumental system; metanephros; sensory organ; and skeleton. Orthologous to human ME2 (malic enzyme 2). [provided by Alliance of Genome Resources, Apr 2022]
Expression: Broad expression in large intestine adult (RPKM 31.9), duodenum adult (RPKM 13.7) and 25 other tissues See more
Orthologs: human all
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Genomic context

Location:: 18 E2; 18 49.95 cM

Exon count:: 17

Annotation release	Status	Assembly	Chr	Location
RS_2024_02	current	GRCm39 (GCF_000001635.27)	18	NC_000084.7 (73898025..73949326, complement)
108.20200622	previous assembly	GRCm38.p6 (GCF_000001635.26)	18	NC_000084.6 (73764954..73815438, complement)

Chromosome 18 - NC_000084.7 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Expression

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See details

Project title: Mouse ENCODE transcriptome data
Description: RNA profiling data sets generated by the Mouse ENCODE project.
BioProject: PRJNA66167
Publication: PMID 25409824
Analysis date: n/a

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Inhibition of miR-214-3p attenuates ferroptosis in myocardial infarction via regulating ME2.
Title: Inhibition of miR-214-3p attenuates ferroptosis in myocardial infarction via regulating ME2.
Cellular redox homeostasis maintained by malic enzyme 2 is essential for MYC-driven T cell lymphomagenesis.
Title: Cellular redox homeostasis maintained by malic enzyme 2 is essential for MYC-driven T cell lymphomagenesis.
Malic enzyme 2 connects the Krebs cycle intermediate fumarate to mitochondrial biogenesis.
Title: Malic enzyme 2 connects the Krebs cycle intermediate fumarate to mitochondrial biogenesis.
deletion of malic enzyme 2 confers collateral lethality in pancreatic cancer
Title: Genomic deletion of malic enzyme 2 confers collateral lethality in pancreatic cancer.
p53 represses the expression of the tricarboxylic-acid-cycle-associated malic enzymes ME1 and ME2 in human and mouse cells
Title: Reciprocal regulation of p53 and malic enzymes modulates metabolism and senescence.
Me2 activity in Islet of Langerhans cells was measured by a spectrophotometric enzyme assay by utilizing the distinct kinetic properties of Me2.
Title: Mitochondrial malic enzyme (ME2) in pancreatic islets of the human, rat and mouse and clonal insulinoma cells.

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Variation

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Alleles

Alleles of this type are documented at Mouse Genome Informatics (MGI)

Gene trapped (1)
Targeted (2)
Chemically induced (ENU) (1)
Endonuclease-mediated (1)

Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Markers

Me2 (e-PCR), detects polymorphism
- UniSTS:546856

AW120568 (e-PCR)
- UniSTS:181151

Gene Ontology Provided by MGI

Function	Evidence Code	Pubs
enables NAD binding	IEA Inferred from Electronic Annotation more info
enables catalytic activity	IEA Inferred from Electronic Annotation more info
enables malate dehydrogenase (decarboxylating) (NAD+) activity	IDA Inferred from Direct Assay more info	PubMed
enables malate dehydrogenase (decarboxylating) (NADP+) activity	IBA Inferred from Biological aspect of Ancestor more info
enables malic enzyme activity	ISO Inferred from Sequence Orthology more info
enables metal ion binding	IEA Inferred from Electronic Annotation more info
enables oxaloacetate decarboxylase activity	IEA Inferred from Electronic Annotation more info
enables oxidoreductase activity	IEA Inferred from Electronic Annotation more info
enables oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor	IEA Inferred from Electronic Annotation more info

Process	Evidence Code	Pubs
involved_in malate metabolic process	IBA Inferred from Biological aspect of Ancestor more info
acts_upstream_of_or_within metabolic process	IEA Inferred from Electronic Annotation more info
involved_in pyruvate metabolic process	IBA Inferred from Biological aspect of Ancestor more info
involved_in pyruvate metabolic process	IDA Inferred from Direct Assay more info	PubMed
involved_in regulation of NADP metabolic process	ISO Inferred from Sequence Orthology more info

Component	Evidence Code	Pubs
located_in intracellular membrane-bounded organelle	ISO Inferred from Sequence Orthology more info
is_active_in mitochondrial matrix	IDA Inferred from Direct Assay more info	PubMed
located_in mitochondrion	HDA more info	PubMed
is_active_in mitochondrion	IBA Inferred from Biological aspect of Ancestor more info
located_in mitochondrion	ISO Inferred from Sequence Orthology more info

General protein information

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Preferred Names: NAD-dependent malic enzyme, mitochondrial

NP_663469.1

EC 1.1.1.38

XP_006525559.1

EC 1.1.1.38

XP_036016815.1

EC 1.1.1.38

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_145494.3 → NP_663469.1 NAD-dependent malic enzyme, mitochondrial precursor

See identical proteins and their annotated locations for NP_663469.1

Status: VALIDATED

Source sequence(s)

AC132418

Consensus CDS

CCDS29339.1

UniProtKB/Swiss-Prot

Q3TBM8, Q99KE1

UniProtKB/TrEMBL

Q3TSD4

Related

ENSMUSP00000025439.4, ENSMUST00000025439.5

Conserved Domains (3) summary

cd05312
Location:280 → 558

NAD_bind_1_malic_enz; NAD(P) binding domain of malic enzyme (ME), subgroup 1

PLN03129
Location:28 → 566

PLN03129; NADP-dependent malic enzyme; Provisional

pfam00390
Location:89 → 270

malic; Malic enzyme, N-terminal domain

RNA

NR_157387.1 RNA Sequence

Status: VALIDATED

Source sequence(s)

AC132418, AK077746

RefSeqs of Annotated Genomes: GCF_000001635.27-RS_2024_02

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCm39 C57BL/6J

Genomic

NC_000084.7 Reference GRCm39 C57BL/6J

Range

73898025..73949326 complement

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

XM_036160922.1 → XP_036016815.1 NAD-dependent malic enzyme, mitochondrial isoform X2

Conserved Domains (1) summary

PLN03129
Location:1 → 390

PLN03129; NADP-dependent malic enzyme; Provisional
XM_006525496.4 → XP_006525559.1 NAD-dependent malic enzyme, mitochondrial isoform X1

UniProtKB/TrEMBL

Q3TSD4

Conserved Domains (1) summary

PLN03129
Location:35 → 573

PLN03129; NADP-dependent malic enzyme; Provisional