CENPE centromere protein E [Homo sapiens (human)] - Gene

Summary

Official Symbol: CENPEprovided by HGNC
Official Full Name: centromere protein Eprovided by HGNC
Primary source: HGNC:HGNC:1856
See related: Ensembl:ENSG00000138778 MIM:117143; AllianceGenome:HGNC:1856
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Homo sapiens
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo
Also known as: KIF10; CENP-E; MCPH13; PPP1R61
Summary: Centrosome-associated protein E (CENPE) is a kinesin-like motor protein that accumulates in the G2 phase of the cell cycle. Unlike other centrosome-associated proteins, it is not present during interphase and first appears at the centromere region of chromosomes during prometaphase. This protein is required for stable spindle microtubule capture at kinetochores which is a necessary step in chromosome alignment during prometaphase. This protein also couples chromosome position to microtubule depolymerizing activity. Alternative splicing results in multiple transcript variants encoding distinct protein isoforms. [provided by RefSeq, Nov 2014]
Expression: Broad expression in bone marrow (RPKM 3.4), lymph node (RPKM 3.3) and 18 other tissues See more
Orthologs: mouse all
NEW: Try the new Gene table
Try the new Transcript table

Genomic context

Location:: 4q24

Exon count:: 49

Annotation release	Status	Assembly	Chr	Location
RS_2023_10	current	GRCh38.p14 (GCF_000001405.40)	4	NC_000004.12 (103105811..103198343, complement)
RS_2023_10	current	T2T-CHM13v2.0 (GCF_009914755.1)	4	NC_060928.1 (106417702..106510204, complement)
105.20220307	previous assembly	GRCh37.p13 (GCF_000001405.25)	4	NC_000004.11 (104026968..104119500, complement)

Chromosome 4 - NC_000004.12 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

Go to the top of the page Help

Go to reference sequence details

Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Expression

Go to the top of the page Help

See details

Project title: HPA RNA-seq normal tissues
Description: RNA-seq was performed of tissue samples from 95 human individuals representing 27 different tissues in order to determine tissue-specificity of all protein-coding genes
BioProject: PRJEB4337
Publication: PMID 24309898
Analysis date: Wed Apr 4 07:08:55 2018

Bibliography

Go to the top of the page Help

GeneRIFs: Gene References Into Functions

What's a GeneRIF?

A farnesyl-dependent structural role for CENP-E in expansion of the fibrous corona.
Title: A farnesyl-dependent structural role for CENP-E in expansion of the fibrous corona.
CENP-E activation by Aurora A and B controls kinetochore fibrous corona disassembly.
Title: CENP-E activation by Aurora A and B controls kinetochore fibrous corona disassembly.
Poly-SUMO-2/3 chain modification of Nuf2 facilitates CENP-E kinetochore localization and chromosome congression during mitosis.
Title: Poly-SUMO-2/3 chain modification of Nuf2 facilitates CENP-E kinetochore localization and chromosome congression during mitosis.
Centromere protein E as a novel biomarker and potential therapeutic target for retinoblastoma.
Title: Centromere protein E as a novel biomarker and potential therapeutic target for retinoblastoma.
CENPE, PRC1, TTK, and PLK4 May Play Crucial Roles in the Osteosarcoma Progression.
Title: CENPE, PRC1, TTK, and PLK4 May Play Crucial Roles in the Osteosarcoma Progression.
Structure and comparison of the motor domain of centromere-associated protein E.
Title: Structure and comparison of the motor domain of centromere-associated protein E.
Mitotic motor CENP-E cooperates with PRC1 in temporal control of central spindle assembly.
Title: Mitotic motor CENP-E cooperates with PRC1 in temporal control of central spindle assembly.
A non-canonical function for Centromere-associated protein-E controls centrosome integrity and orientation of cell division.
Title: A non-canonical function for Centromere-associated protein-E controls centrosome integrity and orientation of cell division.
Kinesin Family Member C1 (KIFC1) Regulated by Centrosome Protein E (CENPE) Promotes Proliferation, Migration, and Epithelial-Mesenchymal Transition of Ovarian Cancer.
Title: Kinesin Family Member C1 (KIFC1) Regulated by Centrosome Protein E (CENPE) Promotes Proliferation, Migration, and Epithelial-Mesenchymal Transition of Ovarian Cancer.
Reduced expression of CENP-E contributes to the development of hepatocellular carcinoma and is associated with adverse clinical features.
Title: Reduced expression of CENP-E contributes to the development of hepatocellular carcinoma and is associated with adverse clinical features.

Submit: New GeneRIF Correction See all GeneRIFs (58)

Phenotypes

Go to the top of the page Help

Find tests for this gene in the NIH Genetic Testing Registry (GTR)

Review eQTL and phenotype association data in this region using PheGenI

Associated conditions

Description	Tests
Microcephaly 13, primary, autosomal recessive MedGen: C4015080 OMIM: 616051 GeneReviews: Not available	Compare labs

EBI GWAS Catalog

Description
Identification of nine new susceptibility loci for testicular cancer, including variants near DAZL and PRDM14. EBI GWAS Catalog EBI GWAS CatalogPubMed

Variation

Go to the top of the page Help

See variants in ClinVar

See studies and variants in dbVar

See Variation Viewer (GRCh37.p13)

See Variation Viewer (GRCh38)

Interactions

Go to the top of the page Help

Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

Go to the top of the page Help

Markers

STS-Z15005 (e-PCR)
- UniSTS:12315

RH123874 (e-PCR)
- UniSTS:136249

SHGC-50720 (e-PCR)
- UniSTS:81211

STS-H95785 (e-PCR)
- UniSTS:47436

SHGC-10601 (e-PCR)
- UniSTS:42075

SHGC-23114 (e-PCR)
- UniSTS:36446

SHGC-23104 (e-PCR)
- UniSTS:2701

D4S2810 (e-PCR)
- UniSTS:10197

Gene Ontology Provided by GOA

Function	Evidence Code	Pubs
enables ATP binding	IEA Inferred from Electronic Annotation more info
enables kinetochore binding	IDA Inferred from Direct Assay more info	PubMed
enables microtubule binding	IBA Inferred from Biological aspect of Ancestor more info
enables microtubule binding	IDA Inferred from Direct Assay more info	PubMed
enables microtubule motor activity	IDA Inferred from Direct Assay more info	PubMed
enables microtubule motor activity	IMP Inferred from Mutant Phenotype more info	PubMed
enables protein binding	IPI Inferred from Physical Interaction more info	PubMed

Process	Evidence Code	Pubs
involved_in attachment of mitotic spindle microtubules to kinetochore	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in cell division	IEA Inferred from Electronic Annotation more info
involved_in chromosome segregation	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in kinetochore assembly	IDA Inferred from Direct Assay more info	PubMed
involved_in lateral attachment of mitotic spindle microtubules to kinetochore	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in metaphase chromosome alignment	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in microtubule plus-end directed mitotic chromosome migration	IDA Inferred from Direct Assay more info	PubMed
involved_in microtubule-based movement	IBA Inferred from Biological aspect of Ancestor more info
involved_in mitotic cell cycle	IBA Inferred from Biological aspect of Ancestor more info
involved_in mitotic cell cycle	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in mitotic chromosome movement towards spindle pole	IDA Inferred from Direct Assay more info	PubMed
involved_in mitotic metaphase chromosome alignment	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in mitotic spindle organization	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in positive regulation of protein kinase activity	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in regulation of mitotic metaphase/anaphase transition	IMP Inferred from Mutant Phenotype more info	PubMed

Component	Evidence Code	Pubs
located_in chromosome	IDA Inferred from Direct Assay more info	PubMed
located_in chromosome, centromeric region	IDA Inferred from Direct Assay more info	PubMed
located_in condensed chromosome, centromeric region	IDA Inferred from Direct Assay more info	PubMed
located_in cytosol	TAS Traceable Author Statement more info
located_in intercellular bridge	IDA Inferred from Direct Assay more info
located_in intracellular membrane-bounded organelle	IDA Inferred from Direct Assay more info
located_in kinetochore	IDA Inferred from Direct Assay more info	PubMed
located_in kinetochore microtubule	IDA Inferred from Direct Assay more info	PubMed
located_in membrane	HDA more info	PubMed
is_active_in microtubule	IBA Inferred from Biological aspect of Ancestor more info
located_in microtubule	IDA Inferred from Direct Assay more info	PubMed
located_in microtubule cytoskeleton	IDA Inferred from Direct Assay more info
located_in midbody	IDA Inferred from Direct Assay more info	PubMed
located_in mitotic spindle	IDA Inferred from Direct Assay more info
located_in mitotic spindle midzone	IDA Inferred from Direct Assay more info	PubMed
located_in nucleus	IMP Inferred from Mutant Phenotype more info	PubMed
located_in spindle midzone	IDA Inferred from Direct Assay more info	PubMed

General protein information

Go to the top of the page Help

Preferred Names: centromere-associated protein E

Names: Centromere autoantigen E (312kD); centromere protein E, 312kDa; kinesin family member 10; kinesin-7; kinesin-related protein CENPE; protein phosphatase 1, regulatory subunit 61

NCBI Reference Sequences (RefSeq)

Go to the top of the page Help

NEW Try the new Transcript table

RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

Genomic

NG_041798.1 RefSeqGene

Range

5067..97599

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_001286734.2 → NP_001273663.1 centromere-associated protein E isoform 2

Status: REVIEWED

Source sequence(s)

AB209996, AC079919, BQ447425

Consensus CDS

CCDS68768.1

UniProtKB/Swiss-Prot

Q02224

Related

ENSP00000369365.3, ENST00000380026.8

Conserved Domains (6) summary

smart00129
Location:6 → 336

KISc; Kinesin motor, catalytic domain. ATPase

COG0419
Location:345 → 890

SbcC; DNA repair exonuclease SbcCD ATPase subunit [Replication, recombination and repair]

COG1196
Location:1651 → 2453

Smc; Chromosome segregation ATPase [Cell cycle control, cell division, chromosome partitioning]

TIGR04523
Location:635 → 1341

Mplasa_alph_rch; helix-rich Mycoplasma protein

cd01374
Location:6 → 329

KISc_CENP_E; Kinesin motor domain, CENP-E/KIP2-like subgroup

pfam04880
Location:803 → 886

NUDE_C; NUDE protein, C-terminal conserved region
NM_001813.3 → NP_001804.2 centromere-associated protein E isoform 1 precursor

See identical proteins and their annotated locations for NP_001804.2

Status: REVIEWED

Source sequence(s)

AA210728, AA210858, AC079919, AL596934, AU100312, AW499901, BQ231878, BQ434767, BQ447425, CD644410, CN418651, Z15005

Consensus CDS

CCDS34042.1

UniProtKB/Swiss-Prot

A6NKY9, A8K2U7, Q02224, Q4LE75

Related

ENSP00000265148.3, ENST00000265148.9

Conserved Domains (6) summary

smart00129
Location:6 → 336

KISc; Kinesin motor, catalytic domain. ATPase

COG0419
Location:345 → 915

SbcC; DNA repair exonuclease SbcCD ATPase subunit [Replication, recombination and repair]

COG1196
Location:1676 → 2482

Smc; Chromosome segregation ATPase [Cell cycle control, cell division, chromosome partitioning]

TIGR04523
Location:660 → 1366

Mplasa_alph_rch; helix-rich Mycoplasma protein

cd01374
Location:6 → 329

KISc_CENP_E; Kinesin motor domain, CENP-E/KIP2-like subgroup

pfam04880
Location:828 → 911

NUDE_C; NUDE protein, C-terminal conserved region

RefSeqs of Annotated Genomes: GCF_000001405.40-RS_2023_10

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCh38.p14 Primary Assembly

Genomic

NC_000004.12 Reference GRCh38.p14 Primary Assembly

Range

103105811..103198343 complement

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

XM_047449534.1 → XP_047305490.1 centromere-associated protein E isoform X5
XM_047449541.1 → XP_047305497.1 centromere-associated protein E isoform X15
XM_047449533.1 → XP_047305489.1 centromere-associated protein E isoform X4
XM_047449536.1 → XP_047305492.1 centromere-associated protein E isoform X9
XM_011531544.3 → XP_011529846.1 centromere-associated protein E isoform X1

Conserved Domains (6) summary

smart00129
Location:6 → 336

KISc; Kinesin motor, catalytic domain. ATPase

COG0419
Location:345 → 890

SbcC; DNA repair exonuclease SbcCD ATPase subunit [Replication, recombination and repair]

COG1196
Location:1651 → 2457

Smc; Chromosome segregation ATPase [Cell cycle control, cell division, chromosome partitioning]

TIGR04523
Location:635 → 1341

Mplasa_alph_rch; helix-rich Mycoplasma protein

cd01374
Location:6 → 329

KISc_CENP_E; Kinesin motor domain, CENP-E/KIP2-like subgroup

pfam04880
Location:803 → 886

NUDE_C; NUDE protein, C-terminal conserved region
XM_047449535.1 → XP_047305491.1 centromere-associated protein E isoform X8
XM_047449540.1 → XP_047305496.1 centromere-associated protein E isoform X14
XM_047449539.1 → XP_047305495.1 centromere-associated protein E isoform X13
XM_011531546.4 → XP_011529848.1 centromere-associated protein E isoform X3

Conserved Domains (5) summary

smart00129
Location:6 → 336

KISc; Kinesin motor, catalytic domain. ATPase

COG1196
Location:1608 → 2414

Smc; Chromosome segregation ATPase [Cell cycle control, cell division, chromosome partitioning]

TIGR04523
Location:1267 → 1910

Mplasa_alph_rch; helix-rich Mycoplasma protein

cd01374
Location:6 → 329

KISc_CENP_E; Kinesin motor domain, CENP-E/KIP2-like subgroup

pfam04880
Location:828 → 911

NUDE_C; NUDE protein, C-terminal conserved region
XM_011531549.3 → XP_011529851.1 centromere-associated protein E isoform X10

Conserved Domains (7) summary

smart00129
Location:6 → 336

KISc; Kinesin motor, catalytic domain. ATPase

COG0419
Location:345 → 915

SbcC; DNA repair exonuclease SbcCD ATPase subunit [Replication, recombination and repair]

COG1196
Location:1660 → 2357

Smc; Chromosome segregation ATPase [Cell cycle control, cell division, chromosome partitioning]

TIGR02168
Location:1163 → 1981

SMC_prok_B; chromosome segregation protein SMC, common bacterial type

TIGR04523
Location:660 → 1366

Mplasa_alph_rch; helix-rich Mycoplasma protein

cd01374
Location:6 → 329

KISc_CENP_E; Kinesin motor domain, CENP-E/KIP2-like subgroup

pfam04880
Location:828 → 911

NUDE_C; NUDE protein, C-terminal conserved region
XM_017007659.2 → XP_016863148.1 centromere-associated protein E isoform X12
XM_047449538.1 → XP_047305494.1 centromere-associated protein E isoform X11
XM_011531545.3 → XP_011529847.1 centromere-associated protein E isoform X2

Conserved Domains (6) summary

smart00129
Location:6 → 336

KISc; Kinesin motor, catalytic domain. ATPase

COG0419
Location:345 → 915

SbcC; DNA repair exonuclease SbcCD ATPase subunit [Replication, recombination and repair]

COG1196
Location:1637 → 2429

Smc; Chromosome segregation ATPase [Cell cycle control, cell division, chromosome partitioning]

TIGR04523
Location:660 → 1366

Mplasa_alph_rch; helix-rich Mycoplasma protein

cd01374
Location:6 → 329

KISc_CENP_E; Kinesin motor domain, CENP-E/KIP2-like subgroup

pfam04880
Location:828 → 911

NUDE_C; NUDE protein, C-terminal conserved region
XM_011531548.3 → XP_011529850.1 centromere-associated protein E isoform X7

Conserved Domains (6) summary

smart00129
Location:6 → 336

KISc; Kinesin motor, catalytic domain. ATPase

COG0419
Location:345 → 915

SbcC; DNA repair exonuclease SbcCD ATPase subunit [Replication, recombination and repair]

COG1196
Location:1676 → 2408

Smc; Chromosome segregation ATPase [Cell cycle control, cell division, chromosome partitioning]

TIGR04523
Location:660 → 1366

Mplasa_alph_rch; helix-rich Mycoplasma protein

cd01374
Location:6 → 329

KISc_CENP_E; Kinesin motor domain, CENP-E/KIP2-like subgroup

pfam04880
Location:828 → 911

NUDE_C; NUDE protein, C-terminal conserved region
XM_011531547.3 → XP_011529849.1 centromere-associated protein E isoform X6

Conserved Domains (6) summary

smart00129
Location:6 → 336

KISc; Kinesin motor, catalytic domain. ATPase

COG0419
Location:345 → 915

SbcC; DNA repair exonuclease SbcCD ATPase subunit [Replication, recombination and repair]

COG1196
Location:1676 → 2478

Smc; Chromosome segregation ATPase [Cell cycle control, cell division, chromosome partitioning]

TIGR04523
Location:660 → 1366

Mplasa_alph_rch; helix-rich Mycoplasma protein

cd01374
Location:6 → 329

KISc_CENP_E; Kinesin motor domain, CENP-E/KIP2-like subgroup

pfam04880
Location:828 → 911

NUDE_C; NUDE protein, C-terminal conserved region