Ano6 anoctamin 6 [Mus musculus (house mouse)] - Gene

Summary

Official Symbol: Ano6provided by MGI
Official Full Name: anoctamin 6provided by MGI
Primary source: MGI:MGI:2145890
See related: Ensembl:ENSMUSG00000064210 AllianceGenome:MGI:2145890
Gene type: protein coding
RefSeq status: VALIDATED
Organism: Mus musculus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
Also known as: Tmem16f; 2900059G15Rik; F730003B03Rik
Summary: Enables gated channel activity; phospholipid scramblase activity; and protein homodimerization activity. Involved in several processes, including bleb assembly; inorganic ion transmembrane transport; and positive regulation of transport. Acts upstream of or within several processes, including bone mineralization involved in bone maturation; calcium activated phospholipid scrambling; and dendritic cell chemotaxis. Is integral component of plasma membrane. Is expressed in several structures, including alimentary system; nervous system; respiratory system; sensory organ; and viscerocranium. Human ortholog(s) of this gene implicated in Scott syndrome; ankylosing spondylitis; and inflammatory bowel disease. Orthologous to human ANO6 (anoctamin 6). [provided by Alliance of Genome Resources, Apr 2022]
Expression: Ubiquitous expression in large intestine adult (RPKM 36.2), small intestine adult (RPKM 31.3) and 27 other tissues See more
Orthologs: human all
NEW: Try the new Gene table
Try the new Transcript table

Genomic context

Location:: 15 E3- F1; 15 50.66 cM

Exon count:: 24

Annotation release	Status	Assembly	Chr	Location
RS_2024_02	current	GRCm39 (GCF_000001635.27)	15	NC_000081.7 (95688724..95873353)
108.20200622	previous assembly	GRCm38.p6 (GCF_000001635.26)	15	NC_000081.6 (95790843..95975472)

Chromosome 15 - NC_000081.7 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

Go to the top of the page Help

Go to reference sequence details

Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Expression

Go to the top of the page Help

See details

Project title: Mouse ENCODE transcriptome data
Description: RNA profiling data sets generated by the Mouse ENCODE project.
BioProject: PRJNA66167
Publication: PMID 25409824
Analysis date: n/a

Bibliography

Go to the top of the page Help

GeneRIFs: Gene References Into Functions

What's a GeneRIF?

TMEM16F phospholipid scramblase mediates trophoblast fusion and placental development.
Title: TMEM16F phospholipid scramblase mediates trophoblast fusion and placental development.
TMEM16F mediates bystander TCR-CD3 membrane dissociation at the immunological synapse and potentiates T cell activation.
Title: TMEM16F mediates bystander TCR-CD3 membrane dissociation at the immunological synapse and potentiates T cell activation.
A novel mechanism of thrombocytopenia by PS exposure through TMEM16F in sphingomyelin synthase 1 deficiency.
Title: A novel mechanism of thrombocytopenia by PS exposure through TMEM16F in sphingomyelin synthase 1 deficiency.
Anion and Cation Permeability of the Mouse TMEM16F Calcium-Activated Channel.
Title: Anion and Cation Permeability of the Mouse TMEM16F Calcium-Activated Channel.
Ano6 disruption impairs acinar cell regulatory volume decrease and protein secretion in murine submandibular salivary glands.
Title: Ano6 disruption impairs acinar cell regulatory volume decrease and protein secretion in murine submandibular salivary glands.
Spinal Motoneuron TMEM16F Acts at C-boutons to Modulate Motor Resistance and Contributes to ALS Pathogenesis.
Title: Spinal Motoneuron TMEM16F Acts at C-boutons to Modulate Motor Resistance and Contributes to ALS Pathogenesis.
Evidence that polyphenols do not inhibit the phospholipid scramblase TMEM16F.
Title: Evidence that polyphenols do not inhibit the phospholipid scramblase TMEM16F.
Critical Role of Lipid Scramblase TMEM16F in Phosphatidylserine Exposure and Repair of Plasma Membrane after Pore Formation.
Title: Critical Role of Lipid Scramblase TMEM16F in Phosphatidylserine Exposure and Repair of Plasma Membrane after Pore Formation.
TMEM16F-promoted M1 polarization and -inhibited M1 polarization were largely associated with the suppression of ADAM17.
Title: TMEM16F inhibition limits pain-associated behavior and improves motor function by promoting microglia M2 polarization in mice.
Here, the authors reveal features of murine TMEM16F (mTMEM16F) that underlie its function as a lipid scramblase and an ion channel. The cryo-EM data of mTMEM16F in absence and presence of Ca(2+) define the ligand-free closed conformation of the protein and the structure of a Ca(2+)-bound intermediate.
Title: Cryo-EM structures and functional characterization of the murine lipid scramblase TMEM16F.

Submit: New GeneRIF Correction See all GeneRIFs (33)

Variation

Go to the top of the page Help

Alleles

Alleles of this type are documented at Mouse Genome Informatics (MGI)

Gene trapped (3)
Targeted (7) 1 citation
Endonuclease-mediated (8)

Interactions

Go to the top of the page Help

Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

Go to the top of the page Help

Markers

D11Bhm20 (e-PCR), detects polymorphism
- UniSTS:141619

D9Mit34.1 (e-PCR), detects polymorphism
- UniSTS:131903

NoName (e-PCR)
- UniSTS:237383

NoName (e-PCR)
- UniSTS:237384

D10Mit157.2 (e-PCR), detects polymorphism
- UniSTS:126061

10.MMHAP5FLD1.seq (e-PCR)
- UniSTS:123034

AW554778 (e-PCR)
- UniSTS:186038

AI115024 (e-PCR)
- UniSTS:160498

Gene Ontology Provided by MGI

Function	Evidence Code	Pubs
enables calcium-activated cation channel activity	IDA Inferred from Direct Assay more info	PubMed
enables calcium-activated cation channel activity	ISO Inferred from Sequence Orthology more info
enables chloride channel activity	IBA Inferred from Biological aspect of Ancestor more info
enables identical protein binding	IPI Inferred from Physical Interaction more info	PubMed
NOT enables intracellularly calcium-gated chloride channel activity	IDA Inferred from Direct Assay more info	PubMed
enables intracellularly calcium-gated chloride channel activity	IDA Inferred from Direct Assay more info	PubMed
enables intracellularly calcium-gated chloride channel activity	IMP Inferred from Mutant Phenotype more info	PubMed
enables metal ion binding	IEA Inferred from Electronic Annotation more info
enables phospholipid scramblase activity	IDA Inferred from Direct Assay more info	PubMed
enables phospholipid scramblase activity	IMP Inferred from Mutant Phenotype more info	PubMed
enables phospholipid scramblase activity	ISO Inferred from Sequence Orthology more info
enables protein dimerization activity	IEA Inferred from Electronic Annotation more info
enables protein homodimerization activity	IDA Inferred from Direct Assay more info	PubMed
enables voltage-gated chloride channel activity	ISO Inferred from Sequence Orthology more info
enables voltage-gated monoatomic ion channel activity	IDA Inferred from Direct Assay more info	PubMed

Process	Evidence Code	Pubs
involved_in activation of blood coagulation via clotting cascade	ISO Inferred from Sequence Orthology more info
involved_in bleb assembly	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in bleb assembly	ISO Inferred from Sequence Orthology more info
involved_in blood coagulation	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within bone mineralization involved in bone maturation	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within calcium activated galactosylceramide scrambling	IDA Inferred from Direct Assay more info	PubMed
involved_in calcium activated phosphatidylcholine scrambling	IBA Inferred from Biological aspect of Ancestor more info
acts_upstream_of_or_within calcium activated phosphatidylcholine scrambling	IDA Inferred from Direct Assay more info	PubMed
involved_in calcium activated phosphatidylserine scrambling	IBA Inferred from Biological aspect of Ancestor more info
acts_upstream_of_or_within calcium activated phosphatidylserine scrambling	IDA Inferred from Direct Assay more info	PubMed
acts_upstream_of_or_within calcium activated phospholipid scrambling	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in calcium ion transmembrane transport	IGI Inferred from Genetic Interaction more info	PubMed
involved_in calcium ion transmembrane transport	ISO Inferred from Sequence Orthology more info
involved_in chloride transmembrane transport	IBA Inferred from Biological aspect of Ancestor more info
involved_in chloride transmembrane transport	IDA Inferred from Direct Assay more info	PubMed
involved_in chloride transmembrane transport	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in chloride transmembrane transport	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within chloride transport	IDA Inferred from Direct Assay more info	PubMed
acts_upstream_of_or_within chloride transport	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in chloride transport	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within dendritic cell chemotaxis	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within establishment of localization in cell	IDA Inferred from Direct Assay more info	PubMed
acts_upstream_of_or_within establishment of localization in cell	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within lipid transport	IEA Inferred from Electronic Annotation more info
involved_in monoatomic cation transport	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within monoatomic ion transmembrane transport	IEA Inferred from Electronic Annotation more info
acts_upstream_of_or_within monoatomic ion transport	IEA Inferred from Electronic Annotation more info
involved_in negative regulation of cell volume	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in negative regulation of cell volume	ISO Inferred from Sequence Orthology more info
NOT acts_upstream_of_or_within phosphatidylserine exposure on apoptotic cell surface	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in phosphatidylserine exposure on blood platelet	ISO Inferred from Sequence Orthology more info
involved_in phospholipid translocation	IDA Inferred from Direct Assay more info	PubMed
involved_in plasma membrane phospholipid scrambling	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in plasma membrane phospholipid scrambling	ISO Inferred from Sequence Orthology more info
involved_in pore complex assembly	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of apoptotic process	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in positive regulation of bone mineralization	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in positive regulation of bone mineralization	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of endothelial cell apoptotic process	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of monoatomic ion transmembrane transport	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in positive regulation of monoatomic ion transmembrane transport	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of monocyte chemotaxis	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in positive regulation of monocyte chemotaxis	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of phagocytosis, engulfment	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in positive regulation of potassium ion export across plasma membrane	ISO Inferred from Sequence Orthology more info
involved_in purinergic nucleotide receptor signaling pathway	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in purinergic nucleotide receptor signaling pathway	ISO Inferred from Sequence Orthology more info
involved_in regulation of postsynaptic membrane potential	IDA Inferred from Direct Assay more info	PubMed
involved_in regulation of postsynaptic membrane potential	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in sodium ion transmembrane transport	ISO Inferred from Sequence Orthology more info

Component	Evidence Code	Pubs
part_of chloride channel complex	ISO Inferred from Sequence Orthology more info
located_in cholinergic synapse	IC Inferred by Curator more info	PubMed
is_active_in cholinergic synapse	IDA Inferred from Direct Assay more info	PubMed
is_active_in cholinergic synapse	IMP Inferred from Mutant Phenotype more info	PubMed
located_in cytosol	ISO Inferred from Sequence Orthology more info
located_in membrane	IEA Inferred from Electronic Annotation more info
part_of monoatomic ion channel complex	IEA Inferred from Electronic Annotation more info
is_active_in plasma membrane	IBA Inferred from Biological aspect of Ancestor more info
located_in plasma membrane	IDA Inferred from Direct Assay more info	PubMed
located_in plasma membrane	ISO Inferred from Sequence Orthology more info
is_active_in synaptic membrane	IDA Inferred from Direct Assay more info	PubMed

General protein information

Go to the top of the page Help

Preferred Names: anoctamin-6

Names: SCAN channel; small-conductance calcium-activated nonselective cation channel; transmembrane protein 16F

NCBI Reference Sequences (RefSeq)

Go to the top of the page Help

NEW Try the new Transcript table

RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_001253813.1 → NP_001240742.1 anoctamin-6 isoform 1

Status: VALIDATED

Description

Transcript Variant: This variant (1) encodes the longer isoform (1).

Source sequence(s)

AK159411, BC060732, BE853067, BY717873

Consensus CDS

CCDS88829.1

UniProtKB/TrEMBL

A0A2I3BPX3

Related

ENSMUSP00000153954.2, ENSMUST00000227791.2

Conserved Domains (2) summary

pfam04547
Location:309 → 893

Anoctamin; Calcium-activated chloride channel

pfam16178
Location:84 → 306

Anoct_dimer; Dimerization domain of Ca+-activated chloride-channel, anoctamin
NM_175344.4 → NP_780553.2 anoctamin-6 isoform 2

See identical proteins and their annotated locations for NP_780553.2

Status: VALIDATED

Description

Transcript Variant: This variant (2) lacks an in-frame exon in the 5' coding region, compared to variant 1. The resulting isoform (2) is shorter than isoform 1.

Source sequence(s)

BC060732, BE853067, BY717873

Consensus CDS

CCDS37184.1

UniProtKB/Swiss-Prot

Q6P9J9, Q8C242

Related

ENSMUSP00000071770.7, ENSMUST00000071874.8

Conserved Domains (2) summary

pfam04547
Location:288 → 871

Anoctamin; Calcium-activated chloride channel

pfam16178
Location:63 → 285

Anoct_dimer; dimerization domain of Ca+-activated chloride-channel, anoctamin

RefSeqs of Annotated Genomes: GCF_000001635.27-RS_2024_02

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCm39 C57BL/6J

Genomic

NC_000081.7 Reference GRCm39 C57BL/6J

Range

95688724..95873353

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

XM_006520231.5 → XP_006520294.1 anoctamin-6 isoform X1

Conserved Domains (2) summary

pfam04547
Location:295 → 878

Anoctamin; Calcium-activated chloride channel

pfam16178
Location:70 → 292

Anoct_dimer; dimerization domain of Ca+-activated chloride-channel, anoctamin
XM_006520233.5 → XP_006520296.1 anoctamin-6 isoform X4

Conserved Domains (2) summary

pfam04547
Location:274 → 857

Anoctamin; Calcium-activated chloride channel

pfam16178
Location:49 → 271

Anoct_dimer; dimerization domain of Ca+-activated chloride-channel, anoctamin
XM_006520232.5 → XP_006520295.1 anoctamin-6 isoform X2

Conserved Domains (2) summary

pfam04547
Location:290 → 873

Anoctamin; Calcium-activated chloride channel

pfam16178
Location:65 → 287

Anoct_dimer; dimerization domain of Ca+-activated chloride-channel, anoctamin
XM_006520234.2 → XP_006520297.2 anoctamin-6 isoform X3

Conserved Domains (2) summary

pfam04547
Location:276 → 859

Anoctamin; Calcium-activated chloride channel

pfam16178
Location:51 → 273

Anoct_dimer; dimerization domain of Ca+-activated chloride-channel, anoctamin