Ano1 anoctamin 1, calcium activated chloride channel [Mus musculus (house mouse)] - Gene

Summary

Official Symbol: Ano1provided by MGI
Official Full Name: anoctamin 1, calcium activated chloride channelprovided by MGI
Primary source: MGI:MGI:2142149
See related: Ensembl:ENSMUSG00000031075 AllianceGenome:MGI:2142149
Gene type: protein coding
RefSeq status: VALIDATED
Organism: Mus musculus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
Also known as: Tmem16a
Summary: Enables intracellular calcium activated chloride channel activity; protein homodimerization activity; and voltage-gated chloride channel activity. Involved in several processes, including chloride transmembrane transport; detection of temperature stimulus involved in sensory perception of pain; and positive regulation of insulin secretion involved in cellular response to glucose stimulus. Acts upstream of or within regulation of membrane potential. Located in apical plasma membrane and external side of plasma membrane. Is integral component of plasma membrane. Is expressed in several structures, including alimentary system; central nervous system; genitourinary system; respiratory system; and sensory organ. Orthologous to human ANO1 (anoctamin 1). [provided by Alliance of Genome Resources, Apr 2022]
Expression: Broad expression in colon adult (RPKM 33.8), genital fat pad adult (RPKM 27.4) and 19 other tissues See more
Orthologs: human all
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Genomic context

Location:: 7 F5; 7 88.85 cM

Exon count:: 31

Annotation release	Status	Assembly	Chr	Location
RS_2024_02	current	GRCm39 (GCF_000001635.27)	7	NC_000073.7 (144142286..144305762, complement)
108.20200622	previous assembly	GRCm38.p6 (GCF_000001635.26)	7	NC_000073.6 (144588549..144752026, complement)

Chromosome 7 - NC_000073.7 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Expression

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See details

Project title: Mouse ENCODE transcriptome data
Description: RNA profiling data sets generated by the Mouse ENCODE project.
BioProject: PRJNA66167
Publication: PMID 25409824
Analysis date: n/a

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Ano1 regulates embryo transport by modulating intracellular calcium levels in oviduct smooth muscle.
Title: Ano1 regulates embryo transport by modulating intracellular calcium levels in oviduct smooth muscle.
Extracellular glucose and dysfunctional insulin receptor signaling independently upregulate arterial smooth muscle TMEM16A expression.
Title: Extracellular glucose and dysfunctional insulin receptor signaling independently upregulate arterial smooth muscle TMEM16A expression.
Tubular TMEM16A promotes tubulointerstitial fibrosis by suppressing PGC-1alpha-mediated mitochondrial homeostasis in diabetic kidney disease.
Title: Tubular TMEM16A promotes tubulointerstitial fibrosis by suppressing PGC-1α-mediated mitochondrial homeostasis in diabetic kidney disease.
A Pharmacological Investigation of the TMEM16A Currents in Murine Skeletal Myogenic Precursor Cells.
Title: A Pharmacological Investigation of the TMEM16A Currents in Murine Skeletal Myogenic Precursor Cells.
TMEM16A in smooth muscle cells acts as a pacemaker channel in the internal anal sphincter.
Title: TMEM16A in smooth muscle cells acts as a pacemaker channel in the internal anal sphincter.
Researches of calcium-activated chloride channel ANO1 intervening amyotrophic lateral sclerosis progression by activating EGFR and CaMKII signaling.
Title: Researches of calcium-activated chloride channel ANO1 intervening amyotrophic lateral sclerosis progression by activating EGFR and CaMKII signaling.
TMEM16A deficiency in alveolar type 2 epithelial cells protected against endoplasmic reticulum stress-induced ferroptosis during acute lung injury.
Title: TMEM16A deficiency in alveolar type 2 epithelial cells protected against endoplasmic reticulum stress-induced ferroptosis during acute lung injury.
IP3R1 underlies diastolic ANO1 activation and pressure-dependent chronotropy in lymphatic collecting vessels.
Title: IP3R1 underlies diastolic ANO1 activation and pressure-dependent chronotropy in lymphatic collecting vessels.
KCNE1 does not shift TMEM16A from a Ca[2+] dependent to a voltage dependent Cl[-] channel and is not expressed in renal proximal tubule.
Title: KCNE1 does not shift TMEM16A from a Ca(2+) dependent to a voltage dependent Cl(-) channel and is not expressed in renal proximal tubule.
Hepatocyte-specific TMEM16A deficiency alleviates hepatic ischemia/reperfusion injury via suppressing GPX4-mediated ferroptosis.
Title: Hepatocyte-specific TMEM16A deficiency alleviates hepatic ischemia/reperfusion injury via suppressing GPX4-mediated ferroptosis.

Submit: New GeneRIF Correction See all GeneRIFs (136)

Variation

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Alleles

Alleles of this type are documented at Mouse Genome Informatics (MGI)

Targeted (9) 1 citation
Endonuclease-mediated (1)

General gene information

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Markers

Tmem16a (e-PCR), detects polymorphism
- UniSTS:498454

D7Mit223 (e-PCR), detects polymorphism
- UniSTS:131116

Tmem16a (e-PCR), detects polymorphism
- UniSTS:507438

RH70659 (e-PCR)
- UniSTS:31301

NoName (e-PCR)
- UniSTS:482419

D7Mit293 (e-PCR), detects polymorphism
- UniSTS:131192

Tmem16a (e-PCR), detects polymorphism
- UniSTS:507282

Tmem16a (e-PCR), detects polymorphism
- UniSTS:498360

AW559012 (e-PCR)
- UniSTS:179687

AU040576 (e-PCR)
- UniSTS:159726

Gene Ontology Provided by MGI

Function	Evidence Code	Pubs
enables calcium-activated cation channel activity	ISO Inferred from Sequence Orthology more info
enables chloride channel activity	IDA Inferred from Direct Assay more info	PubMed
enables chloride channel activity	IMP Inferred from Mutant Phenotype more info	PubMed
enables chloride channel activity	ISO Inferred from Sequence Orthology more info
enables identical protein binding	IPI Inferred from Physical Interaction more info	PubMed
enables identical protein binding	ISO Inferred from Sequence Orthology more info
enables intracellularly calcium-gated chloride channel activity	IBA Inferred from Biological aspect of Ancestor more info
enables intracellularly calcium-gated chloride channel activity	IDA Inferred from Direct Assay more info	PubMed
enables intracellularly calcium-gated chloride channel activity	ISO Inferred from Sequence Orthology more info
enables iodide transmembrane transporter activity	ISO Inferred from Sequence Orthology more info
enables metal ion binding	IEA Inferred from Electronic Annotation more info
NOT enables phospholipid scramblase activity	IDA Inferred from Direct Assay more info	PubMed
enables protein binding	IPI Inferred from Physical Interaction more info	PubMed
enables protein dimerization activity	IEA Inferred from Electronic Annotation more info
enables protein homodimerization activity	IDA Inferred from Direct Assay more info	PubMed
enables signaling receptor binding	ISO Inferred from Sequence Orthology more info
enables voltage-gated chloride channel activity	IDA Inferred from Direct Assay more info	PubMed

Process	Evidence Code	Pubs
involved_in cellular response to heat	IDA Inferred from Direct Assay more info	PubMed
involved_in cellular response to peptide	ISO Inferred from Sequence Orthology more info
involved_in chloride transmembrane transport	IBA Inferred from Biological aspect of Ancestor more info
involved_in chloride transmembrane transport	IDA Inferred from Direct Assay more info	PubMed
involved_in chloride transmembrane transport	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in chloride transmembrane transport	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within chloride transport	IDA Inferred from Direct Assay more info	PubMed
involved_in chloride transport	IDA Inferred from Direct Assay more info	PubMed
involved_in chloride transport	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in chloride transport	ISO Inferred from Sequence Orthology more info
NOT involved_in detection of mechanical stimulus involved in sensory perception of pain	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in detection of temperature stimulus involved in sensory perception of pain	IGI Inferred from Genetic Interaction more info	PubMed
involved_in detection of temperature stimulus involved in sensory perception of pain	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within establishment of localization in cell	IDA Inferred from Direct Assay more info	PubMed
involved_in glial cell projection elongation	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in iodide transport	ISO Inferred from Sequence Orthology more info
involved_in monoatomic cation transport	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within monoatomic ion transmembrane transport	IEA Inferred from Electronic Annotation more info
acts_upstream_of_or_within monoatomic ion transport	IEA Inferred from Electronic Annotation more info
involved_in mucus secretion	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in mucus secretion	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within multicellular organism development	IEA Inferred from Electronic Annotation more info
involved_in phospholipase C-activating G protein-coupled receptor signaling pathway	IDA Inferred from Direct Assay more info	PubMed
involved_in positive regulation of insulin secretion involved in cellular response to glucose stimulus	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in positive regulation of insulin secretion involved in cellular response to glucose stimulus	ISO Inferred from Sequence Orthology more info
involved_in protein localization to membrane	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within regulation of membrane potential	IGI Inferred from Genetic Interaction more info	PubMed
acts_upstream_of_or_within regulation of membrane potential	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in trachea development	IMP Inferred from Mutant Phenotype more info	PubMed

Component	Evidence Code	Pubs
located_in apical plasma membrane	IDA Inferred from Direct Assay more info	PubMed
located_in apical plasma membrane	ISO Inferred from Sequence Orthology more info
located_in cell projection	IEA Inferred from Electronic Annotation more info
part_of chloride channel complex	IEA Inferred from Electronic Annotation more info
located_in external side of plasma membrane	IDA Inferred from Direct Assay more info	PubMed
located_in glutamatergic synapse	IC Inferred by Curator more info	PubMed
is_active_in glutamatergic synapse	IDA Inferred from Direct Assay more info	PubMed
located_in membrane	IEA Inferred from Electronic Annotation more info
located_in nucleoplasm	ISO Inferred from Sequence Orthology more info
is_active_in plasma membrane	IBA Inferred from Biological aspect of Ancestor more info
located_in plasma membrane	IDA Inferred from Direct Assay more info	PubMed
located_in plasma membrane	ISO Inferred from Sequence Orthology more info
located_in plasma membrane	TAS Traceable Author Statement more info
is_active_in presynaptic membrane	IDA Inferred from Direct Assay more info	PubMed
located_in synapse	IEA Inferred from Electronic Annotation more info

General protein information

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Preferred Names: anoctamin-1

Names: transmembrane protein 16A

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_001242349.2 → NP_001229278.2 anoctamin-1 isoform 2

Status: VALIDATED

Source sequence(s)

AC122336, AC153792

Consensus CDS

CCDS57600.1

UniProtKB/TrEMBL

A0A348BSL7

Related

ENSMUSP00000113899.4, ENSMUST00000118556.9

Conserved Domains (2) summary

pfam04547
Location:320 → 896

Anoctamin; Calcium-activated chloride channel

pfam16178
Location:54 → 317

Anoct_dimer; dimerization domain of Ca+-activated chloride-channel, anoctamin
NM_001420255.1 → NP_001407184.1 anoctamin-1 isoform 3

Status: VALIDATED

Source sequence(s)

AC122336, AC153792
NM_178642.6 → NP_848757.5 anoctamin-1 isoform 1

Status: VALIDATED

Source sequence(s)

AC122336, AC153792

Consensus CDS

CCDS40202.2

UniProtKB/Swiss-Prot

Q6P5C6, Q8BHY3, Q8BI26, Q99JK1

UniProtKB/TrEMBL

A0A348BSL7

Related

ENSMUSP00000112616.4, ENSMUST00000121758.8

Conserved Domains (2) summary

pfam04547
Location:320 → 900

Anoctamin; Calcium-activated chloride channel

pfam16178
Location:54 → 317

Anoct_dimer; dimerization domain of Ca+-activated chloride-channel, anoctamin

RefSeqs of Annotated Genomes: GCF_000001635.27-RS_2024_02

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCm39 C57BL/6J

Genomic

NC_000073.7 Reference GRCm39 C57BL/6J

Range

144142286..144305762 complement

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

XM_006508463.5 → XP_006508526.1 anoctamin-1 isoform X8

UniProtKB/TrEMBL

A0A348BSL7

Conserved Domains (2) summary

pfam04547
Location:366 → 972

Anoctamin; Calcium-activated chloride channel

pfam16178
Location:78 → 363

Anoct_dimer; dimerization domain of Ca+-activated chloride-channel, anoctamin
XM_006508459.5 → XP_006508522.1 anoctamin-1 isoform X4

UniProtKB/TrEMBL

A0A0A0MQF2

Conserved Domains (2) summary

pfam04547
Location:399 → 1005

Anoctamin; Calcium-activated chloride channel

pfam16178
Location:111 → 396

Anoct_dimer; dimerization domain of Ca+-activated chloride-channel, anoctamin
XM_006508458.5 → XP_006508521.1 anoctamin-1 isoform X2

UniProtKB/TrEMBL

A0A0A0MQF2

Conserved Domains (2) summary

pfam04547
Location:400 → 1006

Anoctamin; Calcium-activated chloride channel

pfam16178
Location:112 → 397

Anoct_dimer; dimerization domain of Ca+-activated chloride-channel, anoctamin
XM_030241958.2 → XP_030097818.1 anoctamin-1 isoform X6

UniProtKB/TrEMBL

A0A0A0MQF2

Conserved Domains (2) summary

pfam04547
Location:377 → 983

Anoctamin; Calcium-activated chloride channel

pfam16178
Location:111 → 374

Anoct_dimer; dimerization domain of Ca+-activated chloride-channel, anoctamin
XM_030241960.2 → XP_030097820.1 anoctamin-1 isoform X7

UniProtKB/TrEMBL

G8JL82

Conserved Domains (2) summary

pfam04547
Location:399 → 975

Anoctamin; Calcium-activated chloride channel

pfam16178
Location:111 → 396

Anoct_dimer; dimerization domain of Ca+-activated chloride-channel, anoctamin
XM_030241957.2 → XP_030097817.1 anoctamin-1 isoform X5

UniProtKB/TrEMBL

A0A0A0MQF2

Conserved Domains (2) summary

pfam04547
Location:378 → 984

Anoctamin; Calcium-activated chloride channel

pfam16178
Location:112 → 375

Anoct_dimer; dimerization domain of Ca+-activated chloride-channel, anoctamin
XM_017321913.3 → XP_017177402.1 anoctamin-1 isoform X12

UniProtKB/TrEMBL

A0A0A0MQF2

Conserved Domains (2) summary

pfam04547
Location:400 → 980

Anoctamin; Calcium-activated chloride channel

pfam16178
Location:112 → 397

Anoct_dimer; dimerization domain of Ca+-activated chloride-channel, anoctamin
XM_006508464.4 → XP_006508527.1 anoctamin-1 isoform X10

UniProtKB/TrEMBL

A0A348BSL7

Conserved Domains (2) summary

pfam04547
Location:342 → 948

Anoctamin; Calcium-activated chloride channel

pfam16178
Location:54 → 339

Anoct_dimer; dimerization domain of Ca+-activated chloride-channel, anoctamin
XM_011241973.4 → XP_011240275.3 anoctamin-1 isoform X1

UniProtKB/TrEMBL

A0A348BSL7

Conserved Domains (2) summary

pfam04547
Location:342 → 944

Anoctamin; Calcium-activated chloride channel

pfam16178
Location:54 → 339

Anoct_dimer; dimerization domain of Ca+-activated chloride-channel, anoctamin
XM_030241955.2 → XP_030097815.2 anoctamin-1 isoform X3

UniProtKB/TrEMBL

A0A348BSL7, A0A5F8MPM2

Related

ENSMUSP00000159034.3, ENSMUST00000238848.2

Conserved Domains (2) summary

pfam04547
Location:320 → 922

Anoctamin; Calcium-activated chloride channel

pfam16178
Location:54 → 317

Anoct_dimer; dimerization domain of Ca+-activated chloride-channel, anoctamin
XM_011241976.3 → XP_011240278.1 anoctamin-1 isoform X11

Conserved Domains (1) summary

pfam04547
Location:1 → 586

Anoctamin; Calcium-activated chloride channel