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ZBTB33 zinc finger and BTB domain containing 33 [ Homo sapiens (human) ]

Gene ID: 10009, updated on 5-Mar-2024

Summary

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Official Symbol
ZBTB33provided by HGNC
Official Full Name
zinc finger and BTB domain containing 33provided by HGNC
Primary source
HGNC:HGNC:16682
See related
Ensembl:ENSG00000177485 MIM:300329; AllianceGenome:HGNC:16682
Gene type
protein coding
RefSeq status
REVIEWED
Organism
Homo sapiens
Lineage
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo
Also known as
ZNF348; ZNF-kaiso
Summary
This gene encodes a transcriptional regulator with bimodal DNA-binding specificity, which binds to methylated CGCG and also to the non-methylated consensus KAISO-binding site TCCTGCNA. The protein contains an N-terminal POZ/BTB domain and 3 C-terminal zinc finger motifs. It recruits the N-CoR repressor complex to promote histone deacetylation and the formation of repressive chromatin structures in target gene promoters. It may contribute to the repression of target genes of the Wnt signaling pathway, and may also activate transcription of a subset of target genes by the recruitment of catenin delta-2 (CTNND2). Its interaction with catenin delta-1 (CTNND1) inhibits binding to both methylated and non-methylated DNA. It also interacts directly with the nuclear import receptor Importin-α2 (also known as karyopherin alpha2 or RAG cohort 1), which may mediate nuclear import of this protein. Alternatively spliced transcript variants encoding the same protein have been identified.[provided by RefSeq, May 2010]
Expression
Ubiquitous expression in skin (RPKM 10.2), bone marrow (RPKM 8.2) and 25 other tissues See more
Orthologs
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Genomic context

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Location:
Xq24
Exon count:
3
Annotation release Status Assembly Chr Location
RS_2023_10 current GRCh38.p14 (GCF_000001405.40) X NC_000023.11 (120250812..120258398)
RS_2023_10 current T2T-CHM13v2.0 (GCF_009914755.1) X NC_060947.1 (118625869..118633458)
105.20220307 previous assembly GRCh37.p13 (GCF_000001405.25) X NC_000023.10 (119384667..119392253)

Chromosome X - NC_000023.11Genomic Context describing neighboring genes Neighboring gene eukaryotic translation elongation factor 1 alpha 1 pseudogene 30 Neighboring gene Sharpr-MPRA regulatory region 12959 Neighboring gene H3K27ac hESC enhancer GRCh37_chrX:119384374-119385212 Neighboring gene NFKB activating protein pseudogene 1 Neighboring gene transmembrane protein 255A Neighboring gene prefoldin subunit 4-like Neighboring gene NANOG hESC enhancer GRCh37_chrX:119408635-119409312 Neighboring gene ATAC-STARR-seq lymphoblastoid active region 29903 Neighboring gene OCT4-NANOG hESC enhancer GRCh37_chrX:119466056-119466798 Neighboring gene NANOG hESC enhancer GRCh37_chrX:119474477-119474999 Neighboring gene ATPase Na+/K+ transporting family member beta 4

Genomic regions, transcripts, and products

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Go to nucleotide: Graphics FASTA GenBank

Expression

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  • Project title: HPA RNA-seq normal tissues
  • Description: RNA-seq was performed of tissue samples from 95 human individuals representing 27 different tissues in order to determine tissue-specificity of all protein-coding genes
  • BioProject: PRJEB4337
  • Publication: PMID 24309898
  • Analysis date: Wed Apr 4 07:08:55 2018

Bibliography

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Related articles in PubMed

  1. Expressional variations of Kaiso: an association with pathological characteristics and field cancerization of OSCC. Ahmed S, et al. BMC Cancer, 2022 Sep 17. PMID 36115941, Free PMC Article
  2. A Conformational Switch in the Zinc Finger Protein Kaiso Mediates Differential Readout of Specific and Methylated DNA Sequences. Nikolova EN, et al. Biochemistry, 2020 May 26. PMID 32352758, Free PMC Article
  3. Temporal and differential regulation of KAISO-controlled transcription by phosphorylated and acetylated p53 highlights a crucial regulatory role of apoptosis. Choi SH, et al. J Biol Chem, 2019 Aug 30. PMID 31296660, Free PMC Article
  4. CH···O Hydrogen Bonds Mediate Highly Specific Recognition of Methylated CpG Sites by the Zinc Finger Protein Kaiso. Nikolova EN, et al. Biochemistry, 2018 Apr 10. PMID 29546986, Free PMC Article
  5. Kaiso is highly expressed in TNBC tissues of women of African ancestry compared to Caucasian women. Bassey-Archibong BI, et al. Cancer Causes Control, 2017 Nov. PMID 28887687, Free PMC Article

See all (121) citations in PubMed

GeneRIFs: Gene References Into Functions

What's a GeneRIF?
  1. Expressional variations of Kaiso: an association with pathological characteristics and field cancerization of OSCC.
    Title: Expressional variations of Kaiso: an association with pathological characteristics and field cancerization of OSCC.
  2. Kaiso phosphorylation at threonine 606 leads to its accumulation in the cytoplasm, reducing its transcriptional repression of the tumour suppressor CDH1.
    Title: Kaiso phosphorylation at threonine 606 leads to its accumulation in the cytoplasm, reducing its transcriptional repression of the tumour suppressor CDH1.
  3. A Conformational Switch in the Zinc Finger Protein Kaiso Mediates Differential Readout of Specific and Methylated DNA Sequences.
    Title: A Conformational Switch in the Zinc Finger Protein Kaiso Mediates Differential Readout of Specific and Methylated DNA Sequences.
  4. The critical apoptosis regulator KAISO is a p53 target gene that is differently regulated by phosphorylated p53 or acetylated p53, depending on DNA damage responses stage.
    Title: Temporal and differential regulation of KAISO-controlled transcription by phosphorylated and acetylated p53 highlights a crucial regulatory role of apoptosis.
  5. Kaiso plays a role in regulating intestinal epithelial cell integrity and function
    Title: Kaiso-induced intestinal inflammation is preceded by diminished E-cadherin expression and intestinal integrity.
  6. Kaiso modifies MTG16-driven inflammation and tumorigenesis; this suggests that Kaiso deregulation contributes to MTG16-dependent colitis and Colitis-associated carcinoma phenotypes.
    Title: Kaiso is required for MTG16-dependent effects on colitis-associated carcinoma.
  7. EGFR-Kaiso signaling axis is a critical regulator of the miR-200-ZEB1 feedback loop promoting epithelial to mesenchymal transition and metastasis in prostate cancer.
    Title: Transcriptional repressor Kaiso promotes epithelial to mesenchymal transition and metastasis in prostate cancer through direct regulation of miR-200c.
  8. Genomic editing of Kaiso by conversion of K42 into R42 (K42R) in HEK293 cells that resulted in fully deSUMOylated endogenous protein led to misregulation of genes associated with ion transport, blood pressure, and the immune response.
    Title: DeSUMOylation switches Kaiso from activator to repressor upon hyperosmotic stress.
  9. that Kaiso can inhibit endothelial apoptosis via differentially regulating expression of BCL2, BAX, and BIK and that methylated DNA and specific Kaiso binding site (KBS) contribute to the gene regulatory activity of Kaiso.
    Title: Kaiso protects human umbilical vein endothelial cells against apoptosis by differentially regulating the expression of B-cell CLL/lymphoma 2 family members.
  10. The level of miR-4262 was found to be significantly decreased in CC tissues and cell lines. Moreover, decreased expression of miR-4262 was closely related to increased expression of Kaiso (ZBTB33).
    Title: Upregulation of MicroRNA-4262 Targets Kaiso (ZBTB33) to Inhibit the Proliferation and EMT of Cervical Cancer Cells.
Submit: New GeneRIF Correction See all GeneRIFs (53)

Phenotypes

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Find tests for this gene in the NIH Genetic Testing Registry (GTR)

Review eQTL and phenotype association data in this region using PheGenI

Variation

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See variants in ClinVar

See studies and variants in dbVar

See Variation Viewer (GRCh37.p13)

See Variation Viewer (GRCh38)

Genotypes

Pathways from PubChem

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Interactions

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Products Interactant Other Gene Complex Source Pubs Description

General gene information

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Markers

Homology

Gene Ontology Provided by GOA

Function Evidence Code Pubs
enables DNA-binding transcription factor activity, RNA polymerase II-specific ISA
Inferred from Sequence Alignment
more info
  
enables DNA-binding transcription repressor activity, RNA polymerase II-specific IBA
Inferred from Biological aspect of Ancestor
more info
  
enables RNA polymerase II cis-regulatory region sequence-specific DNA binding IBA
Inferred from Biological aspect of Ancestor
more info
  
enables metal ion binding IEA
Inferred from Electronic Annotation
more info
  
enables methyl-CpG binding IDA
Inferred from Direct Assay
more info
 PubMed 
enables protein binding IPI
Inferred from Physical Interaction
more info
 PubMed 
enables sequence-specific DNA binding IDA
Inferred from Direct Assay
more info
 PubMed 
enables sequence-specific double-stranded DNA binding IDA
Inferred from Direct Assay
more info
 PubMed 
Process Evidence Code Pubs
involved_in Wnt signaling pathway IEA
Inferred from Electronic Annotation
more info
  
involved_in intracellular signal transduction TAS
Traceable Author Statement
more info
 PubMed 
involved_in negative regulation of DNA-templated transcription IDA
Inferred from Direct Assay
more info
 PubMed 
involved_in negative regulation of DNA-templated transcription IMP
Inferred from Mutant Phenotype
more info
 PubMed 
involved_in negative regulation of transcription by RNA polymerase II IBA
Inferred from Biological aspect of Ancestor
more info
  
involved_in regulation of cytokine production IBA
Inferred from Biological aspect of Ancestor
more info
  
involved_in regulation of immune system process IBA
Inferred from Biological aspect of Ancestor
more info
  
Component Evidence Code Pubs
part_of chromatin ISA
Inferred from Sequence Alignment
more info
  
located_in cytosol IDA
Inferred from Direct Assay
more info
  
located_in nucleolus IDA
Inferred from Direct Assay
more info
  
is_active_in nucleoplasm IBA
Inferred from Biological aspect of Ancestor
more info
  
located_in nucleoplasm IDA
Inferred from Direct Assay
more info
  
located_in nucleus TAS
Traceable Author Statement
more info
 PubMed 
located_in plasma membrane IDA
Inferred from Direct Assay
more info
  

General protein information

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Preferred Names
transcriptional regulator Kaiso
Names
WUGSC:H_DJ525N14.1
kaiso transcription factor
zinc finger and BTB domain-containing protein 33

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

Genomic

  1. NG_021334.1 RefSeqGene

    Range
    5061..12647
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. NM_001184742.2 → NP_001171671.1  transcriptional regulator Kaiso

    See identical proteins and their annotated locations for NP_001171671.1

    Status: REVIEWED

    Description
    Transcript Variant: This variant (1) is the longer transcript. Variants 1 and 2 encode the same protein.
    Source sequence(s)
    AC002086, AK312321, BC042753, DB468333
    Consensus CDS
    CCDS14596.1
    UniProtKB/Swiss-Prot
    B2R5U6, O00319, Q7Z361, Q86T24, Q8IVP6, Q8N3P0
    Related
    ENSP00000450969.1, ENST00000557385.2
    Conserved Domains (4) summary
    smart00225
    Location:33 → 116
    BTB; Broad-Complex, Tramtrack and Bric a brac
    sd00017
    Location:496 → 516
    ZF_C2H2; C2H2 Zn finger [structural motif]
    pfam00651
    Location:22 → 116
    BTB; BTB/POZ domain
    pfam13465
    Location:508 → 531
    zf-H2C2_2; Zinc-finger double domain

  2. NM_006777.4 → NP_006768.1  transcriptional regulator Kaiso

    See identical proteins and their annotated locations for NP_006768.1

    Status: REVIEWED

    Description
    Transcript Variant: This variant (2) lacks an exon in the 5' UTR, as compared to variant 1.
    Source sequence(s)
    AC002086, BC042753, BX538016
    Consensus CDS
    CCDS14596.1
    UniProtKB/Swiss-Prot
    B2R5U6, O00319, Q7Z361, Q86T24, Q8IVP6, Q8N3P0
    Related
    ENSP00000314153.2, ENST00000326624.2
    Conserved Domains (4) summary
    smart00225
    Location:33 → 116
    BTB; Broad-Complex, Tramtrack and Bric a brac
    sd00017
    Location:496 → 516
    ZF_C2H2; C2H2 Zn finger [structural motif]
    pfam00651
    Location:22 → 116
    BTB; BTB/POZ domain
    pfam13465
    Location:508 → 531
    zf-H2C2_2; Zinc-finger double domain

RefSeqs of Annotated Genomes: GCF_000001405.40-RS_2023_10

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCh38.p14 Primary Assembly

Genomic

  1. NC_000023.11 Reference GRCh38.p14 Primary Assembly

    Range
    120250812..120258398
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

Alternate T2T-CHM13v2.0

Genomic

  1. NC_060947.1 Alternate T2T-CHM13v2.0

    Range
    118625869..118633458
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)
Nucleotide Protein
Heading Accession and Version
Protein Accession Links
GenPept Link UniProtKB Link
Q86T24.2 GenPept UniProtKB/Swiss-Prot:Q86T24

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