GEO DataSets

Analysis of pancreatic ductal adenocarcinoma (PDAC) tumors from PKT; αSMA-tk+ transgenics injected daily with ganciclovir at 6 weeks of age for 10 days or less to deplete αSMA+ myofibroblasts and treated with anti-CTLA4. Results provide insight into molecular basis of anti-CTLA4 therapy in PDAC.

Organism:

Mus musculus

Type:

Expression profiling by array, count, 2 agent, 2 protocol sets

Platform:

GPL6887

Series:

GSE52812

9 Samples

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(Submitter supplied) This SuperSeries is composed of the SubSeries listed below.

Organism:

Mus musculus

Type:

Expression profiling by array; Expression profiling by high throughput sequencing

Platforms:

GPL15907 GPL6887

4 Samples

Download data: TXT

(Submitter supplied) Analysis of differentially expressed genes in CAF associated with PDAC vs NF. Genetically engineered mice with spontaneous pancreas cancer were generated. Their genotype is Ptfa-cre/+:LSL KrasG12D/+;Tgfrb2flox/flox. Cancer associated fibroblasts were expanded in vitro from the tumors of these mice (CAF). Normal fibroblasts (NF) were also expanded from normal pancreas of mice. The experiement consists in comparing the expression profile of CAF vs. more...

Organism:

Mus musculus

Type:

Expression profiling by high throughput sequencing

Platform:

GPL15907

2 Samples

Download data: TXT, XLSX

(Submitter supplied) Analysis of differentially expressed genes in CAF associated with PDAC vs NF

Organism:

Mus musculus

Type:

Expression profiling by array

Platform:

GPL6887

2 Samples

Download data: TXT

(Submitter supplied) Analysis of myofibroblast ablation at the gene expression level of PDAC tumors.

Organism:

Mus musculus

Type:

Expression profiling by array

Datasets:

GDS5820 GDS5821 GDS5822

Platform:

GPL6887

15 Samples

Download data: TXT

Analysis of pancreatic ductal adenocarcinoma (PDAC) tumors from PKT; αSMA-tk+ transgenics injected daily with ganciclovir at 6 weeks of age for 10 days or less to selectively deplete αSMA+ myofibroblasts. Results provide insight into the role of αSMA+ myofibroblasts in late-stage PDAC.

Organism:

Mus musculus

Type:

Expression profiling by array, count, 2 protocol sets

Platform:

GPL6887

Series:

GSE52812

6 Samples

Download data

Analysis of pancreatic ductal adenocarcinoma (PDAC) tumors from PKT; αSMA-tk+ transgenics injected daily with ganciclovir at 4 to 4.5 weeks of age for 14 days or less to selectively deplete αSMA+ myofibroblasts. Results provide insight into the role of αSMA+ myofibroblasts in early-stage PDAC.

Organism:

Mus musculus

Type:

Expression profiling by array, count, 2 protocol sets

Platform:

GPL6887

Series:

GSE52812

6 Samples

Download data

(Submitter supplied) We employed dual-recombinase genetic mouse models of spontaneous PDAC mice (KPPF;Col1smaKO) to delete Col1 (type I collagen) specifically in myofibroblasts, in comparison with control KPPF mice. Single-cell RNA-sequencing analyses were performed on unfractionated live cell mixtures from pancreatic tumors of KPPF mice and KPPF;Col1smaKO mice, to investigate the impact of myofibroblast-specific Col1 deletion on tumor microenvironment.

Organism:

Mus musculus

Type:

Expression profiling by high throughput sequencing

Platform:

GPL19057

4 Samples

Download data: CSV

(Submitter supplied) RNA sequencing analysis on tumor samples from two groups of mice (1) KPPF;Col1smaKO myfibroblast-collagen1 knockout group and (2) KPPF control group. Collagen1 is the most abundant extracellular matrix protein component in desmoplastic pancreatic tumors. In this study, we establish the transgenic mouse model KPPF;Col1smaKO mice with pancreatic tumors deleted for myofibroblast-derived collagen1. Then we use RNA-seq to examine the global changes of gene expression profile in tumors of KPPF;Col1smaKO mice (as compared to control KPPF mice).

Organism:

Mus musculus

Type:

Expression profiling by high throughput sequencing

Platform:

GPL19057

7 Samples

Download data: TXT

(Submitter supplied) Comparison of global gene expression profilles between control and Meflin(islr)-depleted mouse MSCs

Organism:

Mus musculus

Type:

Expression profiling by array

Platform:

GPL13912

4 Samples

Download data: TXT

(Submitter supplied) Single-agent immunotherapy has achieved limited clinical benefit to date in patients with pancreatic ductal adenocarcinoma (PDAC). This may be a result of the presence of a uniquely immunosuppressive tumor microenvironment (TME). Critical obstacles to immunotherapy in PDAC tumors include a high number of tumor-associated immunosuppressive cells and a uniquely desmoplastic stroma that functions as a barrier to T cell infiltration. more...

Organism:

Mus musculus

Type:

Expression profiling by array

Platform:

GPL21163

12 Samples

Download data: TXT

(Submitter supplied) We report the application of bulk RNAseq of live cells from pancreas of KPC or KPC-OG genetic mice at 6 weeks of age. These sponteneous tumors were unperturbed otherwise until timepoint.

Organism:

Mus musculus

Type:

Expression profiling by high throughput sequencing

Platform:

GPL21493

8 Samples

Download data: TXT

(Submitter supplied) Purpose: The goals of this study were to identify quantitative gene expression differences between macrophages derived from wild type and PI3Kgamma null macrophages Methods: mRNA profiles of MCSF, IL4 and IFNg/LPS stimulated macrophage wild-type (WT) and PI3Kinase gamma knockout (p110g-/-) mice were generated by single read deep sequencing, in triplicate, using Illumina HiSeq2000. The sequence reads that passed quality filters were aligned to mouse transcriptome using the bowtie2 aligner. more...

Organism:

Mus musculus

Type:

Expression profiling by high throughput sequencing

Platform:

GPL13112

18 Samples

Download data: TXT

(Submitter supplied) This study used Illumina single-end RNA-sequencing to examine gene expression differences between 2 mouse-derived pancreatic stellate cell lines (PSC4, PSC5) grown either in 2D monolayers, as 3D quiescent cultures, or as 3D activated transwell cocultures with 2 mouse tumor-derived pancreatic ductal organoid lines (T4, T5). Mouse pancreatic stellate cell (PSC) lines were derived from the pancreata of wild-type C57Bl/6J mice. more...

Organism:

Mus musculus

Type:

Expression profiling by high throughput sequencing

Platform:

GPL13112

8 Samples

Download data: TXT

(Submitter supplied) RNA-Seq analysis reporting the transcriptional changes induced by treatment of mouse pancreatic ductal adenocarcinoma cell lines with thrombin for 24h

Organism:

Mus musculus

Type:

Expression profiling by high throughput sequencing

Platform:

GPL17021

24 Samples

Download data: TXT

(Submitter supplied) This SuperSeries is composed of the SubSeries listed below.

Organism:

Mus musculus

Type:

Expression profiling by array

Platform:

GPL6887

12 Samples

Download data

(Submitter supplied) Pancreatic ductal adenocarcinoma (PDAC) is a lethal malignancy that resists current treatments. To test epigenetic therapy against this cancer we used the DNA demethylating drug 5-aza-2’-deoxycytidine (DAC) in a KrasLSL-G12D; p53LSL-R270H/+; Pdx1-cre; Brca1flex2/flex2 (KPC-Brca1) mouse model of aggressive stroma-rich PDAC. In untreated tumors, we found globally decreased 5-methyl-cytosine (5mC) in malignant epithelial cells and in cancer-associated myofibroblasts (CAFs), and increased amounts of 5-hydroxymethyl-cytosine (5HmC) in CAFs, in progression from pancreatic intraepithelial neoplasia (PanIN) to PDAC. more...

Organism:

Mus musculus

Type:

Expression profiling by array

Platform:

GPL6887

6 Samples

Download data: TXT

(Submitter supplied) Pancreatic ductal adenocarcinoma (PDAC) is a lethal malignancy that resists current treatments. To test epigenetic therapy against this cancer we used the DNA demethylating drug 5-aza-2’-deoxycytidine (DAC) in a KrasLSL-G12D; p53LSL-R270H/+; Pdx1-cre; Brca1flex2/flex2 (KPC-Brca1) mouse model of aggressive stroma-rich PDAC. In untreated tumors, we found globally decreased 5-methyl-cytosine (5mC) in malignant epithelial cells and in cancer-associated myofibroblasts (CAFs), and increased amounts of 5-hydroxymethyl-cytosine (5HmC) in CAFs, in progression from pancreatic intraepithelial neoplasia (PanIN) to PDAC. more...

Organism:

Mus musculus

Type:

Expression profiling by array

Platform:

GPL6887

6 Samples

Download data: TXT

(Submitter supplied) In human patients and in mouse models, immunosuppressive regulatory T cells(Tregs)accumulate in Pancreatic Intraepithelial Neoplasia (PanIN), the precursor lesions to pancreatic cancer. Tregs are considered a potential therapeutic target with the goal to reverse immunosuppression in a number of malignancies, including pancreatic cancer. Here, using a genetically engineered mouse that allows at will depletion of Tregs during pancreatic carcinogenesis, we determined that Treg depletion failed to relieve immunosuppression. more...

Organism:

Mus musculus

Type:

Expression profiling by high throughput sequencing

Platform:

GPL21103

3 Samples

Download data: H5, MTX, RDATA, TSV

(Submitter supplied) Regulatory T cells (Treg) are common in the tumor microenvironment in both human pancreatic cancer and in genetically engineered mouse models of the disease. Previous studies in orthotopic syngeneic models of pancreatic cancer -recapitulated in our own data- indicated that Treg depletion results CD8+ T cell-mediated tumor regression. In human patients and in mouse models, regulatory T cells accumulate during the onset of Pancreatic Intraepithelial Neoplasia (PanIN), the earliest steps of carcinogenesis. more...

Organism:

Mus musculus

Type:

Expression profiling by high throughput sequencing

Platform:

GPL21103

7 Samples

Download data: TXT