GEO DataSets

Analysis of pancreatic islets after injection of diabetogenic BDC CD4 T cells into nondiabetic NOD Rag1-/- recipients, 24 to 48 hrs post-transfer. Results provide insight into molecular mechanisms underlying the T-cell entry induced state of receptivity of islets to subsequent immunological insults.

Organism:

Mus musculus

Type:

Expression profiling by array, count, 2 cell type, 2 genotype/variation, 3 protocol, 3 time sets

Platform:

GPL6246

Series:

GSE26147

13 Samples

Download data: CEL, CHP

(Submitter supplied) In an accompanying paper we found specific localization of diabetogenic T cells only to islets of Langerhans bearing the specific antigen. Instrumental in the specific localization was the presence of intra-islet dendritic cells bearing the β-cell-peptide-MHC complex. Here we report that the entry of diabetogenic CD4 T cells very rapidly triggered inflammatory gene expression changes in islets and vessels by up-regulating chemokines and adhesion molecules. more...

Organism:

Mus musculus

Type:

Expression profiling by array

Datasets:

GDS4115 GDS4116

Platform:

GPL6246

20 Samples

Download data: CEL, CHP

Analysis of islet cells after injection of diabetogenic 3A9 CD4 T cells into nondiabetic B10.BR IP-HEL recipients, 8 to 24 hrs post-transfer. Results provide insight into molecular mechanisms underlying the T-cell entry induced state of receptivity of islets to subsequent immunological insults.

Organism:

Mus musculus

Type:

Expression profiling by array, count, 2 genotype/variation, 2 protocol, 3 time sets

Platform:

GPL6246

Series:

GSE26147

7 Samples

Download data: CEL, CHP

(Submitter supplied) We demonstrate diverse roles of interferon–gamma (IFN-γ) in the induction and regulation of immune-mediated inflammation using a transfer model of autoimmune diabetes. The diabetogenic CD4+BDC2.5 (BDC) T cell clone upon transfer into NOD.scid mice induced destruction of islets of Langerhans leading to diabetes. Administration of a neutralizing antibody to IFN-γ (H22) resulted in long term protection (LTP) from diabetes, with inflammation but persistence of a significant, albeit decreased numbers of β-cells. more...

Organism:

Mus musculus

Type:

Expression profiling by array

Dataset:

GDS4034

Platform:

GPL1261

8 Samples

Download data: CEL

Temporal analysis of microdissected islets of NOD.Scid mice injected with diabetogenic CD4+BDC2.5 (BDC) T cells, then injected with anti-interferon-γ monoclonal antibody which protected the mice from developing diabetes. Results provide insight into the role of INF-γ role in autoimmune diabetes.

Organism:

Mus musculus

Type:

Expression profiling by array, transformed count, 2 agent, 2 protocol, 5 time sets

Platform:

GPL1261

Series:

GSE12389

8 Samples

Download data: CEL

(Submitter supplied) Analysis of gene expression levels in ex-vivo and p79-stimulated splenic CD4+ T cells.

Organism:

Mus musculus

Type:

Expression profiling by array

Platform:

GPL14828

12 Samples

Download data: TXT

(Submitter supplied) BDC2.5/NOD mice were treated with cyclophosphamide to induce type 1 diabetes. Their pancreatic islets were analyzed before treatment (Day 0) and as treatment progressed (Days 1 through 3) Keywords = type I diabetes Keywords = cyclophosphamide Keywords = BDC2.5 Keywords = NOD Keywords = pancreas Keywords = islets Keywords: time-course

Organism:

Mus musculus

Type:

Expression profiling by array

Dataset:

GDS1056

Platform:

GPL81

18 Samples

Download data

Analysis of pancreatic islets from BDC2.5/NOD transgenics after treatment with 200 mg/kg cyclophosphamide (CY) at various time points up to 3 days. CY induces diabetes in BDC2.5/NOD transgenics. Results provide insight into molecular events accompanying the progression from insulitis to diabetes.

Organism:

Mus musculus

Type:

Expression profiling by array, count, 2 agent, 4 time sets

Platform:

GPL81

Series:

GSE2254

18 Samples

Download data

(Submitter supplied) Type 1 diabetes (T1D) is an autoimmune disease triggered by T cell reactivity to protein antigens produced by the β-cells. Here we present a chronological compendium of transcriptional profiles from islets of Langerhans isolated from non-obese diabetic (NOD) mice ranging from 2 wks up to diabetes and compared to controls. Parallel analysis was made of cellular components of the islets. Myeloid cells populated the islets early during development in all mouse strains. more...

Organism:

Mus musculus

Type:

Expression profiling by array

Platform:

GPL6246

57 Samples

Download data: CEL