Similar studies for GEO DataSets (Select 3491) - GEO DataSets

Links from GEO DataSets

Items: 20

Select item 34911.

Aire ectopic expression effect on pancreatic islet beta cells

Analysis of pancreatic islet beta cells ectopically expressing Aire. Aire promotes T cell tolerance by inducing the expression of genes encoding peripheral tissue antigens in medullary epithelial cells of the thymus. Results provide insight into the specificity of Aire's effect on transcription.

Organism:: Mus musculus

Type:: Expression profiling by array, count, 2 cell type, 4 genotype/variation sets

Platform:: GPL1261
Series:: GSE12073
12 Samples

Download data: CEL

DataSet

Accession:: GDS3491

ID:: 3491

PubMed Full text in PMC Similar studies GEO Profiles Analyze DataSet

Select item 2000120732.

Expression data from transgenic Aire expressing pancreatic islets

(Submitter supplied) The aim of this study was to determine the effect of transgenic Aire expression on the transcriptional profile of a tissue that normally does not express Aire: pancreatic islets. The transcriptional profile of transgenic RIP-Aire27 islets was compared to non-transgenic littermate islets as well as to archival NOD thymic medullary epithelial cells (MEC) data. All data were from non-obese diabetic (NOD) mice Keywords: RIP-Aire transgenic vs non-transgenic comparison

Organism:: Mus musculus

Type:: Expression profiling by array

Dataset:: GDS3491
Platform:: GPL1261
12 Samples

Download data: CEL

Series

Accession:: GSE12073

ID:: 200012073

PubMed Full text in PMC Similar studies Analyze with GEO2R

Select item 2000338783.

Expression data from WT and Aire KO thymic medullary epithelial cells (MECs)

(Submitter supplied) The aim of this study is to analyze the transcriptional effects of Aire deficiency in the thymus, using the Affymetrix MoGene platform to analyze variation in exon usage

Organism:: Mus musculus

Type:: Expression profiling by array

Platform:: GPL6246
6 Samples

Download data: CEL

Series

Accession:: GSE33878

ID:: 200033878

PubMed Full text in PMC Similar studies Analyze with GEO2R

Select item 2000085634.

Lymphtoxin pathway effects on thymic medullary epithelial cells

(Submitter supplied) We compared gene expression profiles of lymphotoxin alpha- and lymphtoxin beta receptor-deficient thymic medullary epithelial cells with their wild-type littermates, as well as with Aire-deficient and wild-type littermates. This was done in order to determine whether there was overlap in the effects of lymphotoxin and aire. Keywords: genetic modification

Organism:: Mus musculus

Type:: Expression profiling by array

Platform:: GPL1261
18 Samples

Download data: CEL

Series

Accession:: GSE8563

ID:: 200008563

Select item 2000283935.

Aire regulates transfer of antigen from mTEC to dendritic cells for induction of thymic tolerance

(Submitter supplied) To investigate the role of Aire in thymic selection, we examined the cellular requirements for generation of ovalbumin (OVA)-specific CD4 and CD8 T cells in mice expressing OVA under the control of the rat insulin promoter (RIP). Aire-deficiency reduced the number of mature single positive (SP) OVA-specific CD4+ or CD8+ T cells in the thymus, independent of OVA expression. Importantly, it also contributed in two way to OVA-dependent negative selection depending on the T cell type. more...

Organism:: Mus musculus

Type:: Expression profiling by array

Platform:: GPL6481
6 Samples

Download data: TXT

Series

Accession:: GSE28393

ID:: 200028393

Select item 2000634326.

Genome-wide analysis of the TEC-specific Hipk2 gene knockout in mouse medullary thymic epithelial cells (mTECs)

(Submitter supplied) Analysis of the role of Hipk2 in regulating gene expression in medullary thymic epithelial cells. The whole genome gene signatures of purified mTEC subsets (CD80 low, CD80 high) from TEC-specific Hipk2 knockout mice were compared to mating wildtype control mice (floxed, Cre-). Results provide the up- or down-regulated genes, affected by the Hipk2 gene knockout.

Organism:: Mus musculus

Type:: Expression profiling by array

Platform:: GPL17543
8 Samples

Download data: IDAT, TXT

Series

Accession:: GSE63432

ID:: 200063432

PubMed Full text in PMC Similar studies Analyze with GEO2R

Select item 2000694357.

Genome-wide analysis of TRA (tissue restricted antigen)-specific co-expression groups in mouse medullary thymic epithelial cells (mTECs)

(Submitter supplied) Analysis of gene co-expression patterns in TRA-specific medullary thymic epithelial cell (mTEC) subsets. The whole genome gene signatures of purified mTEC subsets respectively positive for the TRAs Gp2, Pdpn, Cea1, Gad1, Ins2, Tspan8 were compared to their corresponding TRA-negative mTEC subset control. Results provide the enriched and depleted gene expressions in the different subsets.

Organism:: Mus musculus

Type:: Expression profiling by array

Platform:: GPL17543
35 Samples

Download data: IDAT, TXT

Series

Accession:: GSE69435

ID:: 200069435

Select item 2000085648.

Analysis of Aire effects on individual mice of different genetic backgrounds

(Submitter supplied) We compared gene expression profiles of aire-deficient and wild-type littermate thymic medullary epithelial cells. This was done in order to determine whether Aire's effects differed among strains, and also among individuals of the same strain. Keywords: genetic modification

Organism:: Mus musculus

Type:: Expression profiling by array

Platform:: GPL1261
20 Samples

Download data: CEL

Series

Accession:: GSE8564

ID:: 200008564

PubMed Full text in PMC Similar studies Analyze with GEO2R

Select item 2000000859.

wild type and aire -/- murine meduallary thymic epithelial cells

(Submitter supplied) Mice used were B6/129 F2's, 3-5 weeks of age, either wild type or with both copies of the autoimmune regulator gene (aire, GenBank #AF079536) disrupted. Thymi from five of these mice of both sexes were removed and pooled. After collagenase/dispase digestion, density gradient fractionation, and fluorescent antibody staining, cells with the phenotype CD45-, G8.8+, CDR1int and B7.1hi were FACS-sorted and total RNA was made from them. more...

Organism:: Mus musculus

Type:: Expression profiling by array

Dataset:: GDS167
Platform:: GPL81
6 Samples

Download data: CEL

Series

Accession:: GSE85

ID:: 200000085

Select item 16710.

Autoimmune disease mechanisms

Examination of medullary thymic epithelial cells from aire-deficient mice. aire is autoimmune regulator gene. Aim to understand autoimmune disease mechanisms, esp. autoimmune polyendocrinopathy candidiasis ectodermal dystrophy (APECED).

Organism:: Mus musculus

Type:: Expression profiling by array, count, 2 strain sets

Platform:: GPL81
Series:: GSE85
6 Samples

Download data: CEL

DataSet

Accession:: GDS167

ID:: 167

Select item 20009101511.

Crispr-Cas9-mediated Aire gene editing in medullary thymic epithelial (mTEC) cells shows its role as a gene expression modulator during thymocyte adhesion

(Submitter supplied) The aim of this study is to evaluate the effect of Autoimmune regulator (Aire) gene disruption in a murine medullary thymic epithelial cells (mTEC 3.10 cell line) on the transcriptome of these cells during its adhesion with thymocytes. The mTEC-thymocyte adhesion is a crucial step for the negative selection of autoreactive thymocytes and prevention of autoimmune diseases. To generate Aire mutant cell clones, a total of 5x10^5 mTEC 3.10 cells were electro-transfected (Lonza Nucleofector) with CRISPR-Cas9 plasmid targeting the Aire Exon 3 (plasmid "all in one" encoding Aire Exon 3 gRNA + Cas9 + GFP, from Sigma-Aldrich). more...

Organism:: Mus musculus

Type:: Expression profiling by high throughput sequencing

Platform:: GPL17021
8 Samples

Download data: TXT

Series

Accession:: GSE91015

ID:: 200091015

PubMed Full text in PMC Similar studies SRA Run Selector

Select item 20006144412.

Next Generation Sequencing Facilitates Quantitative Analysis of Wild Type and Jmjd6-/- Thymic stromal Transcriptomes

(Submitter supplied) The goals of this study are to comprehensively identify genes controlled by Jmjd6 in the thymic stroma, and to identify a novel alternative splicing mechanism. Methods: Samples were WT and Jmjd6-/- fetal thymus organ culture with (2 samples for each category) or without (1 sample for each category) RANKL stimulation for 4 days under 2-DG. One µg of total RNA was used for library construction with TruSeq RNA Sample Prep Kit v2. more...

Organism:: Mus musculus

Type:: Expression profiling by high throughput sequencing

Platform:: GPL13112
6 Samples

Download data: TXT, XLSX

Series

Accession:: GSE61444

ID:: 200061444

PubMed Full text in PMC Similar studies SRA Run Selector

Select item 20006836313.

Expression data of Sirt1-deficient and sufficient medullary thymic epithelial cells (mTECs)

(Submitter supplied) Medullary thymic epithelial cells play essential role for induction of central self-tolerance. This functional capacity is mediated through a phenomenon known as promiscuous gene expression (pGE) of various tissue-restricted antigen (TRA) genes. pGE was previously shown to be mediated by a single factor called the Autoimmune regulator (Aire), which is specically expressed by mTECs. The aim of this study was to analyze the impact of deacetylase Sirtuin1, which is also highly expressed by mTECs, on mTEC gene expression profile and compare it with the impact of Aire. more...

Organism:: Mus musculus

Type:: Expression profiling by array

Platform:: GPL6246
3 Samples

Download data: CEL

Series

Accession:: GSE68363

ID:: 200068363

Select item 20006819014.

Sirt1 is essential for Aire-mediated induction of central immunological tolerance

(Submitter supplied) Autoimmune regulator (Aire) is a unique transcriptional regulator that induces promiscuous expression of thousands of tissue-restricted antigens (TRAs) in medullary thymic epithelial cells (mTECs), a step critical for induction of immunological self-tolerance. Although several recent studies provided very important molecular insights into how Aire operates, a more comprehensive understanding of this process still remains elusive. more...