Conserved Protein Domain Family
NUT

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pfam12881: NUT 
NUT protein
This family includes the NUT protein. The gene encoding for NUT protein (Nuclear Testis protein) is found fused to BRD3 or BRD4 genes, in some aggressive types of carcinoma, due to chromosomal translocations. Proteins of the BRD family contain two bromodomains that bind transcriptionally active chromatin through associations with acetylated histones H3 and H4. Such proteins are crucial for the regulation of cell cycle progression. On the other hand, little is known about NUT protein. NUT is known to have a Nuclear Export Sequence (NES) as well as a Nuclear localization Signal (NLS), both located towards the C-terminal end of the protein. A fused NUT-GFP protein showed either cytoplasmic or nuclear localization, suggesting that it is subject to nuclear/cytoplasmic shuttling. Consistent with this possibility, treatment with leptomycin B an inhibitor of CRM1-dependent nuclear export resulted in re-distribution of NUT-GFP to the nucleus. Inspection of NUT revealed a C-terminal sequence similar to known nuclear export sequences (NES) which are often regulated by phosphorylation. This family carries some natively unstructured sequence.
Statistics
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PSSM-Id: 432850
Aligned: 9 rows
Threshold Bit Score: 895.756
Created: 24-Mar-2022
Updated: 17-Oct-2022
Structure
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Aligned Rows:
PubMed ReferencesClick to see Conserved Features Help

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
XP_024109232   14 MTVNPGTSLSVFTALPFTTPAPSLAHG-------LPLVTAGVPPGSPLVLS-AFPSTPLVAGQDg--RGPSGAGASNVFV 83  
XP_001054929   27 MTVNAGATMSTFATLPVLPPAPQPAPQlfw-eppAPLMTAGISPRNPLVLS-ALPGMSLVAEDG---STALNAAVPLNIV 101 
4_pfamImport    1 MTLNPDASTPPFIALPFHPPGHGPAYRppwgqqlPPLMTAVFPPRSPLVLS-TLPKTPLVAGDSahcHCPSGAEALSVIL 79  
XP_014591075   14 VTMNPGASMSPFSALPFSPPTPGPPHQpswqqhpLPLITPSFPPDSPLVLP-TFPRTPLVATSG---HSPGGTGACNVIV 89  
XP_005883162   14 VTLKPGASMTPFTAQPFPPPTTGPAHQppgeqhlPPLRTPSFPPGRPLVLP-ALPRTPLVAGDAg--QGPVGPGACNIMD 90   Brandt's bat
XP_024851794    3 VAVTPGASLPPFRTMPFPPPIPGPQHWppweqhlPPSMTPSFPPGGTQLLP-AFPRTPLVPNAG---YGPSAPGACNIIV 78   cattle
XP_003791722   14 MTMKPGATLSPFTALPFPSSTPGPPDQppwepppQAPMPPAFSPGNPLVLS-AFPSPLLVTGDGg--PCPSGAGAGKVIV 90   small-eared g...
XP_023364079   14 MILNPDASMPPFTALPFHPPGPSPALGpprgqqpPPLMTAVFPLGSPFLLS-TLPRTPLVAGDGt--CGPSGAGAFSVIL 90   small-eared g...
XP_017202967    1 MIPHPGTSTSLFTLLPFPQPTHGPAPQppwgqppPLLLNAAFPPGSRLVLPaAIPRMPVVAGDGg--SVPTGAGNVHIIF 78   rabbit
XP_024109232   84 QIGTEMGPANAPQVQTLVLTQAPLVWQAPGTLCGGVVCPPPLLLAAAPVVPVMA------PQVVGGTQAGEGGWSQGLPl 157 
XP_001054929  102 PLGTLGRPVQPVHNANIVLTQVPLNYNIPGAQGVGMGYSTPLFMATPAANTFIN------TRIASAVQPQEGTWVLGPR- 174 
4_pfamImport   80 QVKSEGRSVQAVPTQTFVLPQAPLKWSASGALCRGAPNPVPLLLTAPPG-----------VPVTGGAPTSIGGWITGLP- 147 
XP_014591075   90 QVRLEGRPAEPPQTQNFVLTQASLNWSAPGALCGNAAYSATLFLAASVVETTVP------APAVGGTQAGKAGWSPGLP- 162 
XP_005883162   91 QVRSEGGRPQPSQAQTIVLTQAPPNWNAPGAICGGAVCPAPLFLEASAVETIMP------ASAFGGTQAGDGGWCPGLP- 163  Brandt's bat
XP_024851794   79 QVRSEGRPAEHPQTQTFVLTQAPLNWSTPGPLSRSTACPVPLFLTTPAMETIVT------APAVGVAQSGKGSWTPGFP- 151  cattle
XP_003791722   91 KVKTEGGPAEPSQTQNFFLTQTALNWIASGAPCGGPEDPPPLFVTASNMKTILP------TKAVGTP------------- 151  small-eared g...
XP_023364079   91 QVRSEGELVQAPPNKTFVLPQAPLNWSAPGAFCTGAQGQAPLLLSAPPVETIMPapiigsAPIVGGISASVGGWITGLP- 169  small-eared g...
XP_017202967   79 QLRTGGHPVEPPHSQTLVYNQTQISLNAPGGLCGPGQNPAPIAVPGSSLQATVP------APPMVGTQASQGGWPLGLP- 151  rabbit
XP_024109232  158 PPPPPPAAQLPPIMSQGNAGPWPQGAHGEGSLASSQAKA-LPDDSCNPKSVYENFRLWQHYKPLARRHLPQSPDTEALSC 236 
XP_001054929  175 PPTTQQVVQLVPVRSPVTSAPPPKGAYGESVPANIQARNsPENYLSKPDSVYGNFRRWQHIKTLVQRHLPQTPDVAAFSC 254 
4_pfamImport  148 HLVPPLAAHLTPNVSPVNTRPQPYRECRESSLPLS-----TRDDSCNAKSVYENYQWWQHFKSLAWRHFPQSPDTEAFSC 222 
XP_014591075  163 PRAPPPASQLASTTPPVNAGPHPHGASREGGLATSACKA-SLDDSCTPKSVYENFRRWQRFKSLARRHHPQSPDTEALSC 241 
XP_005883162  164 PQAPPPAAQLAPIVPSVNA-----GASRKGGLATSQSKT-SPDDSCNPNSVYKNFRRWQRFKALARRHLPQSPDAEALCC 237  Brandt's bat
XP_024851794  152 PQAPLPAAQLAPIIPQVNLGPQLHGISREGSLATNQSKA-SQDDSCNPKSVYENFRRWQRFKSLARRHLPQSPDAEALSC 230  cattle
XP_003791722  152 PQVSPPVAQLAPIVPLEKAWPGLHGTTGEGGLAAIQSKPsLGDLSYTSKGVYENFRRWQRYKALARRHFPQSPDAEALSC 231  small-eared g...
XP_023364079  170 RPVPSPATHLAPVVSPVNTGLQPHGMCREGSLPTSQAKA-LQDDSCNPKSVYENYRRWQHFKSLARRHFPQNPDTEAFSC 248  small-eared g...
XP_017202967  152 PPAPPPAAQLAPMVFPANNGPPSQGADRERILPTPKAQA-SPDNPCSSTSVYENFRRWQHFKALARRLLPQSPDTEALSC 230  rabbit
XP_024109232  237 FLIPVLRSLARRKPTMTLEEGLWRAMREWQHTSNFDRMIFYEMAEKFLEFEAEEEMQIQklqwMKGPQCLPPPATSRLEP 316 
XP_001054929  255 FLIPVLRSLARRKPTMNVEEGLWRGLQEWQCTSNYDRMIFFEMAEKFTEFENAEEMENS----MRNIQYQVLATAPRQDP 330 
4_pfamImport  223 FLIPVLRTLARLKPTMTLDEGLWRAVQEWQQKNDFDRMIFYEMAEKFMEFEAEEEMQLQqlkwMKGSNFLPPSAPCRPSP 302 
XP_014591075  242 FLIPVLRSLARLKPAMTLEEGLWRAVQEWQHKSNFDRMIFYEMAGKFMEFEAEEEMQIQklqwMKGARGPPPPAPPRPDP 321 
XP_005883162  238 FLIPVLRSLSRLRPTMTLEEGMWRAMQEWQRKSNFDRMIYYEMAEKFMEFEAEEQMQIQklelKKGAHTQPPPAPPRPDP 317  Brandt's bat
XP_024851794  231 FLIPVLRSLARLKPTMTLEEGLWRAVQEWQHRSNFDRMIYYEMARKFMEFEAEEVMQIQklqwMQGVQGLPPPVPPKLDP 310  cattle
XP_003791722  232 FLIPVLRSLARLKPTMTLEEGLPRAVQEWERTSNFDRMIFYEMAEKFMEFEAEEEMQLQnsqlTNGSQGLSPAASLKLDP 311  small-eared g...
XP_023364079  249 FLIPVLRTLARLKPTMTLDEGLWRAVQEWQQKNNFDRMIFYEMAEKFMEFEAEEEMQLQqlqwMRGSQSLPPPTPPRPDP 328  small-eared g...
XP_017202967  231 FLIPVLRTLAQRRPTMPLEEGLRFAMREWQHKSNFDRMIFYEMAEKFMEFEEEEEMQMQklewMKGKPCQPPPVSPPLLP 310  rabbit
XP_024109232  317 RGPPAPEVVKQPVYLPskagpkaptaclppprpqrpvtkarlppprphRRAETKA---RLPPPRPQ-RPAETKVPEEIPP 392 
XP_001054929  331 PRLPAPKAVKEPECTS--------------------------------MKTVPRTdsaHLPEFRHQ-QLRKAKTPTEIPP 377 
4_pfamImport  303 QDSTASEVVQQPGLHPllssvaswptaapa-----apssylltsvvlpRKVGNKp---HIPPPTPDwQLPENKAPKEIPP 374 
XP_014591075  322 QGPPAPEVGLQPVAsac-----------------------------tpRKAGPRGqpaRPQPHRPQ-RRPESGAPKEIPP 371 
XP_005883162  318 RGPPAPVVGGQPar--------------------------------tpRKALPSAqhiR----RHQ-RPQETKSPSEIPP 360  Brandt's bat
XP_024851794  311 QGSSAPTVCPQPGTHVptgvps---------------------kasapRKAGPRAhptRSQPHRAQ-RHPGTKAPKEIPP 368  cattle
XP_003791722  312 PGPLTPEVCQQSVYIP--------------------------------KKAASKArapRRRQRKPQ-RPPVPEAPKEIPP 358  small-eared g...
XP_023364079  329 QEQPGTKVVQHPvv--------------------------------lpKKADTKAqpiHLPPPTPQqWPPEIKAPKEIPP 376  small-eared g...
XP_017202967  311 QGPPAPVAVQQPGca--------------------------------pRKASSKAlpaCPVPPRHQ--GPQDRTPKEIPP 356  rabbit
XP_024109232  393 EVVQEYMDIMEELLGPSLGATGEPEKQREEGEVKQPQEEDWTPPDPGLLSYIDKLCSQKDFVTKVEAVIHPRFLEELLSP 472 
XP_001054929  378 EAVQEYMDIMDWLEGFPQPFAGDPKEKEEDENSGTEQEGDDLYSDAGLLSYIDELCSQKRFVEQVEAIINPQFVAEILSA 457 
4_pfamImport  375 EVMKEYLKIMDKVLGPPCGDSGELDTQLEEGKAEPPQYEAGTIPDPRFLCYIDKLCTQEDFITKVEAVIHPRFLEELLSA 454 
XP_014591075  372 EAVREYVDIMEGMLGLARPATRELDGGRKEDRNERQQEGGGVYPDLGLLSYIDKLCSQEDFVTKVEAVIHPRFLAELLSP 451 
XP_005883162  361 EAVREYMDIMDNLLGLPHSATEAPGGGWEDEVKEWLQ-------DEGLLSYLDQLCSQEDFVTQVEAVIHPQFMEQIISP 433  Brandt's bat
XP_024851794  369 EAVREYVNIMEVLVGPIHLATGKSEAECGKDEDELKQEEDGTYADPDLLSYIDKLCSQEEFVTKVEAVIHPRFLADLLSP 448  cattle
XP_003791722  359 EAVKEYADIMEGLVG-SHSATGEPEGKQEEEEEQ-QQEEEGMYPDSGLLSYIEDLCSQEIFVSKVEAVIHPQFLADLLSP 436  small-eared g...
XP_023364079  377 EAVREYIDIMDKLLGPPCGDLGKPDAQLEEGEADQSQDEFGIYPDPGLLSYIDKLCSQKDFITKVEAVIHPRFLEELLSP 456  small-eared g...
XP_017202967  357 EAVKEYMEIMDTLLGPTPLSLEPPDGHPEEDTDEGFLENEGSYPDPEMLSYIDHLCSQVDFVTKAEAIIHPRFLEELLSP 436  rabbit
XP_024109232  473 DPQMDFLALSQELEQEEGLTLAQLVEKRLLTLKEKQHTRAAPSHGTAQLDSSSSKFAAGQGAERDIPDPQQGVGMETCPP 552 
XP_001054929  458 KPEMDILALTKELEHEEELTIDQLIEKHCLALKKKGVERAALNPGDPQNPANASVLPACQGAQRDDCGAQKGASTQTASS 537 
4_pfamImport  455 ETNMDSLALTQELEKEEGLTLDQMVEKRLLALKEEQSMGAAQSYQALWVNSSASKSAAGHGTKNNSHRPKLEIVAETYPA 534 
XP_014591075  452 EPQLDPLALAEELEQEEGLTTEQLVQKRLLALKEEEGVQRPPSHSAPPLDSSPSESEAGPDAH--DHDPQLGVSDQAYPP 529 
XP_005883162  434 DTQQDPLALAEELEQEEGLTAAQLVEKRVLASK-EGGVQAPPSHGAPLLNASSSESEIGQDAQRCDRGPQLGVSDKACPP 512  Brandt's bat
XP_024851794  449 EPQLDPLALAEELEQEEGLTPEELVQKRLLALKEDKGVRTPSSQSVPQVDSSPSESDASKDAQRYDQGPQLGVRHEAGPP 528  cattle
XP_003791722  437 EQQRDPLALIEELEQEEGLTLAQLVQKRLLALEEEEDAEAPPSCSGAQSDSSPSVSdededgggrlrplpgprgpggtvr 516  small-eared g...
XP_023364079  457 ETNMDSLALTQELEQEERLTLDQLVEKRLLALKEEQSVGVTLSRRALQMESSPSKLAAGQGAERDSHGPKLGVGAETCPA 536  small-eared g...
XP_017202967  437 DPQLDLTALSKELEKEEGLTAGQVMAKHC-SLKEDGAMGATPSHGVPLLNPSNSESGPQQSAESDNQGPRLGARAECFSR 515  rabbit
XP_024109232  553 QTATRDPQGRGRAHTGMARSKDSVVLLGCQDSPGLRAARPTSPPQDHRPTSPGLGSKDALDLPGGSPVRESHGLAQGSSE 632 
XP_001054929  538 RMASLDQQCLGGTNTELWGPKPATLHPSNQSSPSLVDNGSTGFPCGRGAHNQSPGSRCAGILRGTSATEDPQGPLGKTIE 617 
4_pfamImport  535 QMVSHAPQGHGRVKDDLPRPKAPGLLSWHAESPRLRATQPTSPFQGLSLTTPGLGTRDASGLQGASPVRETFRQEDGSSE 614 
XP_014591075  530 ETDCEDAQRPGQADTDLPRPQALALSPGRQESPPLGAARPPSPPQAPRLTSPTLGSRDAPVLREASPARETHRPADGSSE 609 
XP_005883162  513 ETDFKDQQRRGPAGAHRPRPQASAVSTGHQEHPPFCARCPPSAPQGHGPADCALGSREACVPREASLVRETLEP-DRSSE 591  Brandt's bat
XP_024851794  529 EVDFEAVHRFSQANTGLSKAKDLVPSLGQQELPPFQPGQP-SPPQGQRCTGPQPGPRDTSVLRTVFPILGARGPRDGSSE 607  cattle
XP_003791722  517 lgkasslgkrarevhsgreraldgprglrtdgntlppprswdlpldratpqgtqgplgmerrgsgkvinqissyqdshlg 596  small-eared g...
XP_023364079  537 QMASR-AQGHSRMQADLLRPKARGLLSGHPESPRLRATLPTSPFQGLKPTSLGLGTRDALGLQGASPVRETFGPGDGSSE 615  small-eared g...
XP_017202967  516 SMSSQDLQRHAVPDTPLCRPEVNAVFSGHRECPSLGA------------------TEDARSFRGGPSVGGPHTPLDISSE 577  rabbit
XP_024109232  633 EE-ELPSLAFLLGSQHKLLPWWLPQSPVPASGLLSPEKwgpqgTHQSpSAQRRGLSLAPSPATKSKKRPLFGSPSPAEKT 711 
XP_001054929  618 DKeELHSLSFLLSSQYRLVPWRLSPQMCPYADLPDSAS-----IPKP-CPKMRGPSPDPCPIVKSKKRAVFGGLVPTAKK 691 
4_pfamImport  615 DEeELPSLAFLLASQHRLLPWGLSQNPVPG---------------QPpSAQRRGFSPAAPPMAKSSKQVICGYRLPAERM 679 
XP_014591075  610 DEdDLPSLAFLLASQHSLLPWRLSQSPAPASGLLCPGGqgaqgAPQApSPQSTGLSPAPPAAAESWKRPVCAGPAPAEKM 689 
XP_005883162  592 DEeDLPSLAFLLGSPNSLLPCGLSLSPVPASGLACPGGrgprgAAQSqFFQTLGLTRASPAASKSRKRALAGGPAHAEKT 671  Brandt's bat
XP_024851794  608 DEeELPSLAFLLASQHSLLPWRLSQSPVPASG-------------QApSSQRIGLSPAP--------------------- 653  cattle
XP_003791722  597 gagspglclgvdgtsdalplswqedsqcertpsldaglaepaglqgqglesqvlglptgqkmgglgvlpqgqeplavpqe 676  small-eared g...
XP_023364079  616 DEeELPSLTFLLASQNRLLPWGLSQSPVPV---------------QPpSAQRRGLSSATLPVAKCNKRVLSGDQFHAEKM 680  small-eared g...
XP_017202967  578 NEeELPSLSFLLASQHHLLPLGFAGSEASAEDLPCLGAgnpcrAPKPlSTHRRDPRGPLPHAAKSRKQALQGGQGPAKKK 657  rabbit
XP_024109232  712 PHPGPGLRVSGEQSLTWGLGGPSQSQKRKGDPLVSrKKKKQR 753 
XP_001054929  692 PNLEPGFGVSEEPLSALELVHLSQTQKRNHETLGT-RKRKRK 732 
4_pfamImport  680 AYPGSQLRVSGAKPLALRSGQFLKPQKRRSDPFFSgTEKKQC 721 
XP_014591075  690 PLPGAKLRVPGRSALALGLVRPSQPQKRRCDPCVTgRRRKRH 731 
XP_005883162  672 PRPGANLGVCGRPVLALELVCASQPQKKKCDlavtgrsnk-- 711  Brandt's bat
XP_024851794  654 --LGAGLGVSETPALALGLVRSSQLRKRKCDPFVTgRKRKRL 693  cattle
XP_003791722  677 dgsgaawgdgnglpmapsynqnpspraagdrdgsslspelwl 718  small-eared galago
XP_023364079  681 ASPGPELGASGGEPLALGPGRSSQPQKRKGDSFVSgMKKKQR 722  small-eared galago
XP_017202967  658 PCPVPQLRASAGQAQALGSVGNSQAQKRKGDHLVTrRRKKHH 699  rabbit
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