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Two directionally cloned cDNA libraries were made from fetal liver hematopoietic stem cells enriched to be Lineageneg/lo, AA4.1pos, cKitpos, Ly6A/E (Sca-1)pos called Scapos and from AA4.1neg fetal liver cells. Subtractive hybridization was performed by hybridization of the target, Scapos, single stranded cDNA library in pSport1 to biotinylated RNA transcribed from the driver, AA4.1neg cDNA library in pSport2 with inserts cloned in the complementary orientation. For detailed protocols and additional information please see our website at http://stemcell.princeton.edu.
Nucleotide
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