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UI-E-DX1

Identifiers
BioSample: SAMN00170173; EST: LIBEST_010278
Organism
Homo sapiens (human)
cellular organisms; Eukaryota; Opisthokonta; Metazoa; Eumetazoa; Bilateria; Deuterostomia; Chordata; Craniata; Vertebrata; Gnathostomata; Teleostomi; Euteleostomi; Sarcopterygii; Dipnotetrapodomorpha; Tetrapoda; Amniota; Mammalia; Theria; Eutheria; Boreoeutheria; Euarchontoglires; Primates; Haplorrhini; Simiiformes; Catarrhini; Hominoidea; Hominidae; Homininae; Homo
Attributes
tissuefetal eyes
development stagefetal
lab hostDH10B (Life Technologies) (T1 phage resistant)
tissueeye
vectorpT7T3-Pac (Pharmacia) with a modified polylinker
v_typePlasmid
re_1EcoR I
re_2Not I
Description

UI-E-DX1 is a normalized cDNA library containing the following tissue(s): fetal eyes. The library was constructed according to Bonaldo, Lennon and Soares, Genome Research, 6:791-806, 1996. First strand cDNA synthesis was primed with an oligo-dT primer containing a Not I site. Double stranded cDNA was ligated to an EcoR I adaptor, digested with Not I, and cloned directionally into pT7T3-Pac vector. The oligonucleotide used to prime the synthesis of first-strand cDNA contains a library tag sequence that is located between the Not I site and the (dT)18 tail. The sequence tag for this library is AGAATCAAGA. This library was created for the program, Gene Discovery in the Visual System, supported by National Eye Institute (NEI).

Submission
Children's Memorial Research Center, Soares, MB; 2002-02-27
Accession:
SAMN00170173
ID:
170173

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