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6AH0: The Cryo-EM Structure of the Precusor of Human Pre-catalytic Spliceosome (pre-B complex)

PDB ID:	6AH0 Download
MMDB ID:	168638
PDB Deposition Date:	2018/8/15
Updated in MMDB:	2018/12
Experimental Method:	electron microscopy
Resolution:	5.7 Å
Source Organism:	Homo sapiens
Similar Structures:

Biological Unit for 6AH0: 57-meric; determined by author

Molecular Components in 6AH0
Label	Count	Molecule
Proteins (52 molecules)
D	1	U5 Small Nuclear Ribonucleoprotein 200 KDA Helicase (Gene symbol: SNRNP200)
E	1	U5 Small Nuclear Ribonucleoprotein 40 KDA Protein (Gene symbol: SNRNP40)
a k P	3	Small Nuclear Ribonucleoprotein SM D2 (Gene symbol: SNRPD2)
b m Q	3	Small Nuclear Ribonucleoprotein F (Gene symbol: SNRPF)
c l R	3	Small Nuclear Ribonucleoprotein E (Gene symbol: SNRPE)
d n S	3	Small Nuclear Ribonucleoprotein G (Gene symbol: SNRPG)
e h T	3	Small Nuclear Ribonucleoprotein SM D3 (Gene symbol: SNRPD3)
f i U	3	Small Nuclear Ribonucleoprotein-associated Proteins B and B' (Gene symbol: SNRPB)
g j V	3	Small Nuclear Ribonucleoprotein SM D1 (Gene symbol: SNRPD1)
q	1	U6 Snrna-associated Sm-like Protein Lsm2 (Gene symbol: LSM2)
r	1	U6 Snrna-associated Sm-like Protein Lsm3 (Gene symbol: LSM3)
s	1	U6 Snrna-associated Sm-like Protein Lsm4 (Gene symbol: LSM4)
t	1	U6 Snrna-associated Sm-like Protein Lsm5 (Gene symbol: LSM5)
x	1	U6 Snrna-associated Sm-like Protein Lsm6 (Gene symbol: LSM6)
y	1	U6 Snrna-associated Sm-like Protein Lsm7 (Gene symbol: LSM7)
z	1	U6 Snrna-associated Sm-like Protein Lsm8 (Gene symbol: LSM8)
o	1	U2 Small Nuclear Ribonucleoprotein A' (Gene symbol: SNRPA1)
p	1	U2 Small Nuclear Ribonucleoprotein B'' (Gene symbol: SNRPB2)
u	1	Splicing Factor 3A Subunit 1 (Gene symbol: SF3A1)
v	1	Splicing Factor 3A Subunit 2 (Gene symbol: SF3A2)
w	1	Splicing Factor 3A Subunit 3 (Gene symbol: SF3A3)
1	1	Splicing Factor 3B Subunit 1 (Gene symbol: SF3B1)
2	1	Splicing Factor 3B Subunit 2 (Gene symbol: SF3B2)
3	1	Splicing Factor 3B Subunit 3 (Gene symbol: SF3B3)
4	1	Splicing Factor 3B Subunit 4 (Gene symbol: SF3B4)
5	1	Splicing Factor 3B Subunit 6 (Gene symbol: SF3B6)
6	1	PHD Finger-like Domain-containing Protein 5A (Gene symbol: PHF5A)
7	1	Splicing Factor 3B Subunit 5 (Gene symbol: SF3B5)
J	1	U4/u6 Small Nuclear Ribonucleoprotein Prp3 (Gene symbol: PRPF3)
K	1	U4/u6 Small Nuclear Ribonucleoprotein Prp4 (Gene symbol: PRPF4)
N	1	Pre-mrna-processing Factor 6 (Gene symbol: PRPF6)
L	1	U4/u6 Small Nuclear Ribonucleoprotein Prp31 (Gene symbol: PRPF31)
M	1	Nhp2-like Protein 1 (Gene symbol: SNU13)
O	1	Thioredoxin-like Protein 4A (Gene symbol: TXNL4A)
W	1	U4/u6.u5 Tri-snrnp-associated Protein 2 (Gene symbol: USP39)
X	1	Probable Atp-dependent RNA Helicase Ddx23 (Gene symbol: DDX23)
A	1	Pre-mrna-processing-splicing Factor 8 (Gene symbol: PRPF8)
C	1	116 KDA U5 Small Nuclear Ribonucleoprotein Component (Gene symbol: EFTUD2)
Nucleotides(5 molecules)
B	1	U5snrna
F	1	U6snrna
G	1	Pre-mrna
H	1	U2snrna
I	1	U4snrna
Chemicals and Non-standard biopolymers (3 molecules)
1	1	INOSITOL HEXAKISPHOSPHATE
2	1	GUANOSINE-5'-TRIPHOSPHATE
3	1	MAGNESIUM ION

* Click molecule labels to explore molecular sequence information.

Citing MMDB

Madej T, Lanczycki CJ, Zhang D, Thiessen PA, Geer RC, Marchler-Bauer A, Bryant SH. " MMDB and VAST+: tracking structural similarities between macromolecular complexes. Nucleic Acids Res. 2014 Jan; 42(Database issue):D297-303