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Conserved domains on  [gi|1720396581|ref|XP_030102203|]
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eF-hand calcium-binding domain-containing protein 8 isoform X4 [Mus musculus]

Protein Classification

Graphical summary

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List of domain hits

 Name Accession Description Interval E-value
WD40 super family cl29593
WD40 domain, found in a number of eukaryotic proteins that cover a wide variety of functions ...
 322-614 1.53e-25

WD40 domain, found in a number of eukaryotic proteins that cover a wide variety of functions including adaptor/regulatory modules in signal transduction, pre-mRNA processing and cytoskeleton assembly; typically contains a GH dipeptide 11-24 residues from its N-terminus and the WD dipeptide at its C-terminus and is 40 residues long, hence the name WD40; between GH and WD lies a conserved core; serves as a stable propeller-like platform to which proteins can bind either stably or reversibly; forms a propeller-like structure with several blades where each blade is composed of a four-stranded anti-parallel b-sheet; instances with few detectable copies are hypothesized to form larger structures by dimerization; each WD40 sequence repeat forms the first three strands of one blade and the last strand in the next blade; the last C-terminal WD40 repeat completes the blade structure of the first WD40 repeat to create the closed ring propeller-structure; residues on the top and bottom surface of the propeller are proposed to coordinate interactions with other proteins and/or small ligands; 7 copies of the repeat are present in this alignment.


The actual alignment was detected with superfamily member cd00200:

Pssm-ID: 475233 [Multi-domain]  Cd Length: 289  Bit Score: 108.19  E-value: 1.53e-25
                           10        20        30        40        50        60        70        80
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1720396581  322 HPNWCQQVKYIPQLNVVASCSAVEKsslvlIILPGKEPEKPRLSSLNLRKGILCFDYCPDRNFLATGGYDPHIRLWNpLV 401
Cdd:cd00200      8 HTGGVTCVAFSPDGKLLATGSGDGT-----IKVWDLETGELLRTLKGHTGPVRDVAASADGTYLASGSSDKTIRLWD-LE 81

                           90       100       110       120       130       140       150       160
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1720396581  402 SKKPVWLMKGHQTSVTHLLVnSRNASILISISRDKNIRVWDLQDYVCLQSFCGKlfplgNCPITSTYFYRSSTLICSTYN 481
Cdd:cd00200     82 TGECVRTLTGHTSYVSSVAF-SPDGRILSSSSRDKTIKVWDVETGKCLTTLRGH-----TDWVNSVAFSPDGTFVASSSQ 155

                          170       180       190       200       210       220       230       240
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1720396581  482 IGILRGY-LESQGPLKTgrMTTHNTPLCAVLYSKIFKQVVSGCLQGLVSVWDISTGKKRMEIPVSgiqESELTAMALDQS 560
Cdd:cd00200    156 DGTIKLWdLRTGKCVAT--LTGHTGEVNSVAFSPDGEKLLSSSSDGTIKLWDLSTGKCLGTLRGH---ENGVNSVAFSPD 230

                          250       260       270       280       290
                   ....*....|....*....|....*....|....*....|....*....|....*.
gi 1720396581  561 ERCLLTGLRDGTMQMWNYNTGECLMTFPNPDRvEISGIVHMN--KVFYTTGWSKRI 614
Cdd:cd00200    231 GYLLASGSEDGTIRVWDLRTGECVQTLSGHTN-SVTSLAWSPdgKRLASGSADGTI 285
EF-hand_8 pfam13833
EF-hand domain pair;
67-119 1.50e-03

EF-hand domain pair;


:

Pssm-ID: 404678 [Multi-domain]  Cd Length: 54  Bit Score: 37.68  E-value: 1.50e-03
                           10        20        30        40        50
                   ....*....|....*....|....*....|....*....|....*....|....
gi 1720396581   67 ENGALKKEGFIRIMK-GVLSSMSEEMLELLFLKVDSDCNGFVTWQKYVDYMMRE 119
Cdd:pfam13833    1 EKGVITREELKRALAlLGLKDLSEDEVDILFREFDTDGDGYISFDEFCVLLERR 54
KLF3_N super family cl40586
N-terminal domain of Kruppel-like factor 3; Kruppel-like factor 3 (KLF3; also called ...
 1173-1231 5.52e-03

N-terminal domain of Kruppel-like factor 3; Kruppel-like factor 3 (KLF3; also called Krueppel-like factor 3 and originally called Basic Kruppel-like Factor/BKLF), was the third member of the KLF family of zinc finger transcription factors to be discovered. KLF3 possesses a wide range of biological impacts on regulating apoptosis, differentiation, and proliferation in various tissues during the entire progression process. It has been proposed as a tumor suppressor in colorectal cancer. It appears to function predominantly as a repressor of transcription, turning genes off by recruiting the C-terminal Binding Protein co-repressors CtBP1 and CtBP2. CtBP docks onto a short motif (residues 61-65) in the N-terminus of KLF3, through the Proline-X-Aspartate-Leucine-Serine (PXDLS) motif. CtBP in turn recruits histone modifying enzymes to alter chromatin and repress gene expression. KLF3 belongs to a family of proteins, called the Specificity Protein (SP)/KLF family, characterized by a C-terminal DNA-binding domain of 81 amino acids consisting of three Kruppel-like C2H2 zinc fingers. These factors bind to a loose consensus motif, namely NNRCRCCYY (where N is any nucleotide; R is A/G, and Y is C/T), such as the recurring motifs in GC and GT boxes (5'-GGGGCGGGG-3' and 5-GGTGTGGGG-3') that are present in promoters and more distal regulatory elements of mammalian genes. Members of the KLF family can act as activators or repressors of transcription depending on cell and promoter context. KLFs regulate various cellular functions, such as proliferation, differentiation, and apoptosis, as well as the development and homeostasis of several types of tissue. In addition to the C-terminal DNA-binding domain, each KLF also has a unique N-terminal activation/repression domain that confers specificity and allows it to bind specifically to a certain partner, leading to distinct activities in vivo. This model represents the N-terminal domain of KLF3.


The actual alignment was detected with superfamily member cd21577:

Pssm-ID: 410554 [Multi-domain]  Cd Length: 214  Bit Score: 39.64  E-value: 5.52e-03
                           10        20        30        40        50        60
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*.
gi 1720396581 1173 IPTDMVAGSPAATPS-------SLSMVSVGQSPSNWSTSLKPLSSTSFLRPSSASPAHSGSSTPPH 1231
Cdd:cd21577      3 VKTDMETSFYSPSHSqlepvdlSLSKRSSPPSSSSSSSSSSSSSSSPSSRASPPSPYSKSSPPSPP 68
 
Name Accession Description Interval E-value
WD40 cd00200
WD40 domain, found in a number of eukaryotic proteins that cover a wide variety of functions ...
 322-614 1.53e-25

WD40 domain, found in a number of eukaryotic proteins that cover a wide variety of functions including adaptor/regulatory modules in signal transduction, pre-mRNA processing and cytoskeleton assembly; typically contains a GH dipeptide 11-24 residues from its N-terminus and the WD dipeptide at its C-terminus and is 40 residues long, hence the name WD40; between GH and WD lies a conserved core; serves as a stable propeller-like platform to which proteins can bind either stably or reversibly; forms a propeller-like structure with several blades where each blade is composed of a four-stranded anti-parallel b-sheet; instances with few detectable copies are hypothesized to form larger structures by dimerization; each WD40 sequence repeat forms the first three strands of one blade and the last strand in the next blade; the last C-terminal WD40 repeat completes the blade structure of the first WD40 repeat to create the closed ring propeller-structure; residues on the top and bottom surface of the propeller are proposed to coordinate interactions with other proteins and/or small ligands; 7 copies of the repeat are present in this alignment.


Pssm-ID: 238121 [Multi-domain]  Cd Length: 289  Bit Score: 108.19  E-value: 1.53e-25
                           10        20        30        40        50        60        70        80
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1720396581  322 HPNWCQQVKYIPQLNVVASCSAVEKsslvlIILPGKEPEKPRLSSLNLRKGILCFDYCPDRNFLATGGYDPHIRLWNpLV 401
Cdd:cd00200      8 HTGGVTCVAFSPDGKLLATGSGDGT-----IKVWDLETGELLRTLKGHTGPVRDVAASADGTYLASGSSDKTIRLWD-LE 81

                           90       100       110       120       130       140       150       160
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1720396581  402 SKKPVWLMKGHQTSVTHLLVnSRNASILISISRDKNIRVWDLQDYVCLQSFCGKlfplgNCPITSTYFYRSSTLICSTYN 481
Cdd:cd00200     82 TGECVRTLTGHTSYVSSVAF-SPDGRILSSSSRDKTIKVWDVETGKCLTTLRGH-----TDWVNSVAFSPDGTFVASSSQ 155

                          170       180       190       200       210       220       230       240
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1720396581  482 IGILRGY-LESQGPLKTgrMTTHNTPLCAVLYSKIFKQVVSGCLQGLVSVWDISTGKKRMEIPVSgiqESELTAMALDQS 560
Cdd:cd00200    156 DGTIKLWdLRTGKCVAT--LTGHTGEVNSVAFSPDGEKLLSSSSDGTIKLWDLSTGKCLGTLRGH---ENGVNSVAFSPD 230

                          250       260       270       280       290
                   ....*....|....*....|....*....|....*....|....*....|....*.
gi 1720396581  561 ERCLLTGLRDGTMQMWNYNTGECLMTFPNPDRvEISGIVHMN--KVFYTTGWSKRI 614
Cdd:cd00200    231 GYLLASGSEDGTIRVWDLRTGECVQTLSGHTN-SVTSLAWSPdgKRLASGSADGTI 285
WD40 COG2319
WD40 repeat [General function prediction only];
380-666 2.08e-20

WD40 repeat [General function prediction only];


Pssm-ID: 441893 [Multi-domain]  Cd Length: 403  Bit Score: 95.36  E-value: 2.08e-20
                           10        20        30        40        50        60        70        80
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1720396581  380 PDRNFLATGGYDPHIRLWNpLVSKKPVWLMKGHQTSVTHLLVnSRNASILISISRDKNIRVWDLQDYVCLQSFCGklfpl 459
Cdd:COG2319    130 PDGKTLASGSADGTVRLWD-LATGKLLRTLTGHSGAVTSVAF-SPDGKLLASGSDDGTVRLWDLATGKLLRTLTG----- 202

                           90       100       110       120       130       140       150       160
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1720396581  460 GNCPITSTYFYRSSTLICSTYNIGILRGY-LESQGPLKTgrMTTHNTPLCAVLYSKIFKQVVSGCLQGLVSVWDISTGKk 538
Cdd:COG2319    203 HTGAVRSVAFSPDGKLLASGSADGTVRLWdLATGKLLRT--LTGHSGSVRSVAFSPDGRLLASGSADGTVRLWDLATGE- 279

                          170       180       190       200       210       220       230       240
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1720396581  539 rmEIPVSGIQESELTAMALDQSERCLLTGLRDGTMQMWNYNTGECLMTFPNPDRvEISGIVHM--NKVFYTTGWSK--RI 614
Cdd:COG2319    280 --LLRTLTGHSGGVNSVAFSPDGKLLASGSDDGTVRLWDLATGKLLRTLTGHTG-AVRSVAFSpdGKTLASGSDDGtvRL 356

                          250       260       270       280       290
                   ....*....|....*....|....*....|....*....|....*....|...
gi 1720396581  615 TNFTFQKITQALscyhwqSFHTEDILSMD-KYQNQFLGTSSYNGDIVFWNVST 666
Cdd:COG2319    357 WDLATGELLRTL------TGHTGAVTSVAfSPDGRTLASGSADGTVRLWDLAT 403
WD40 smart00320
WD40 repeats; Note that these repeats are permuted with respect to the structural repeats ...
 402-442 6.17e-04

WD40 repeats; Note that these repeats are permuted with respect to the structural repeats (blades) of the beta propeller domain.


Pssm-ID: 197651 [Multi-domain]  Cd Length: 40  Bit Score: 38.45  E-value: 6.17e-04
                            10        20        30        40
                    ....*....|....*....|....*....|....*....|.
gi 1720396581   402 SKKPVWLMKGHQTSVTHLLVNSrNASILISISRDKNIRVWD 442
Cdd:smart00320    1 SGELLKTLKGHTGPVTSVAFSP-DGKYLASGSDDGTIKLWD 40
EF-hand_8 pfam13833
EF-hand domain pair;
67-119 1.50e-03

EF-hand domain pair;


Pssm-ID: 404678 [Multi-domain]  Cd Length: 54  Bit Score: 37.68  E-value: 1.50e-03
                           10        20        30        40        50
                   ....*....|....*....|....*....|....*....|....*....|....
gi 1720396581   67 ENGALKKEGFIRIMK-GVLSSMSEEMLELLFLKVDSDCNGFVTWQKYVDYMMRE 119
Cdd:pfam13833    1 EKGVITREELKRALAlLGLKDLSEDEVDILFREFDTDGDGYISFDEFCVLLERR 54
WD40 pfam00400
WD domain, G-beta repeat;
370-398 4.95e-03

WD domain, G-beta repeat;


Pssm-ID: 459801 [Multi-domain]  Cd Length: 39  Bit Score: 35.78  E-value: 4.95e-03
                           10        20
                   ....*....|....*....|....*....
gi 1720396581  370 RKGILCFDYCPDRNFLATGGYDPHIRLWN 398
Cdd:pfam00400   11 TGSVTSLAFSPDGKLLASGSDDGTVKVWD 39
KLF3_N cd21577
N-terminal domain of Kruppel-like factor 3; Kruppel-like factor 3 (KLF3; also called ...
 1173-1231 5.52e-03

N-terminal domain of Kruppel-like factor 3; Kruppel-like factor 3 (KLF3; also called Krueppel-like factor 3 and originally called Basic Kruppel-like Factor/BKLF), was the third member of the KLF family of zinc finger transcription factors to be discovered. KLF3 possesses a wide range of biological impacts on regulating apoptosis, differentiation, and proliferation in various tissues during the entire progression process. It has been proposed as a tumor suppressor in colorectal cancer. It appears to function predominantly as a repressor of transcription, turning genes off by recruiting the C-terminal Binding Protein co-repressors CtBP1 and CtBP2. CtBP docks onto a short motif (residues 61-65) in the N-terminus of KLF3, through the Proline-X-Aspartate-Leucine-Serine (PXDLS) motif. CtBP in turn recruits histone modifying enzymes to alter chromatin and repress gene expression. KLF3 belongs to a family of proteins, called the Specificity Protein (SP)/KLF family, characterized by a C-terminal DNA-binding domain of 81 amino acids consisting of three Kruppel-like C2H2 zinc fingers. These factors bind to a loose consensus motif, namely NNRCRCCYY (where N is any nucleotide; R is A/G, and Y is C/T), such as the recurring motifs in GC and GT boxes (5'-GGGGCGGGG-3' and 5-GGTGTGGGG-3') that are present in promoters and more distal regulatory elements of mammalian genes. Members of the KLF family can act as activators or repressors of transcription depending on cell and promoter context. KLFs regulate various cellular functions, such as proliferation, differentiation, and apoptosis, as well as the development and homeostasis of several types of tissue. In addition to the C-terminal DNA-binding domain, each KLF also has a unique N-terminal activation/repression domain that confers specificity and allows it to bind specifically to a certain partner, leading to distinct activities in vivo. This model represents the N-terminal domain of KLF3.


Pssm-ID: 410554 [Multi-domain]  Cd Length: 214  Bit Score: 39.64  E-value: 5.52e-03
                           10        20        30        40        50        60
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*.
gi 1720396581 1173 IPTDMVAGSPAATPS-------SLSMVSVGQSPSNWSTSLKPLSSTSFLRPSSASPAHSGSSTPPH 1231
Cdd:cd21577      3 VKTDMETSFYSPSHSqlepvdlSLSKRSSPPSSSSSSSSSSSSSSSPSSRASPPSPYSKSSPPSPP 68
 
Name Accession Description Interval E-value
WD40 cd00200
WD40 domain, found in a number of eukaryotic proteins that cover a wide variety of functions ...
 322-614 1.53e-25

WD40 domain, found in a number of eukaryotic proteins that cover a wide variety of functions including adaptor/regulatory modules in signal transduction, pre-mRNA processing and cytoskeleton assembly; typically contains a GH dipeptide 11-24 residues from its N-terminus and the WD dipeptide at its C-terminus and is 40 residues long, hence the name WD40; between GH and WD lies a conserved core; serves as a stable propeller-like platform to which proteins can bind either stably or reversibly; forms a propeller-like structure with several blades where each blade is composed of a four-stranded anti-parallel b-sheet; instances with few detectable copies are hypothesized to form larger structures by dimerization; each WD40 sequence repeat forms the first three strands of one blade and the last strand in the next blade; the last C-terminal WD40 repeat completes the blade structure of the first WD40 repeat to create the closed ring propeller-structure; residues on the top and bottom surface of the propeller are proposed to coordinate interactions with other proteins and/or small ligands; 7 copies of the repeat are present in this alignment.


Pssm-ID: 238121 [Multi-domain]  Cd Length: 289  Bit Score: 108.19  E-value: 1.53e-25
                           10        20        30        40        50        60        70        80
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1720396581  322 HPNWCQQVKYIPQLNVVASCSAVEKsslvlIILPGKEPEKPRLSSLNLRKGILCFDYCPDRNFLATGGYDPHIRLWNpLV 401
Cdd:cd00200      8 HTGGVTCVAFSPDGKLLATGSGDGT-----IKVWDLETGELLRTLKGHTGPVRDVAASADGTYLASGSSDKTIRLWD-LE 81

                           90       100       110       120       130       140       150       160
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1720396581  402 SKKPVWLMKGHQTSVTHLLVnSRNASILISISRDKNIRVWDLQDYVCLQSFCGKlfplgNCPITSTYFYRSSTLICSTYN 481
Cdd:cd00200     82 TGECVRTLTGHTSYVSSVAF-SPDGRILSSSSRDKTIKVWDVETGKCLTTLRGH-----TDWVNSVAFSPDGTFVASSSQ 155

                          170       180       190       200       210       220       230       240
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1720396581  482 IGILRGY-LESQGPLKTgrMTTHNTPLCAVLYSKIFKQVVSGCLQGLVSVWDISTGKKRMEIPVSgiqESELTAMALDQS 560
Cdd:cd00200    156 DGTIKLWdLRTGKCVAT--LTGHTGEVNSVAFSPDGEKLLSSSSDGTIKLWDLSTGKCLGTLRGH---ENGVNSVAFSPD 230

                          250       260       270       280       290
                   ....*....|....*....|....*....|....*....|....*....|....*.
gi 1720396581  561 ERCLLTGLRDGTMQMWNYNTGECLMTFPNPDRvEISGIVHMN--KVFYTTGWSKRI 614
Cdd:cd00200    231 GYLLASGSEDGTIRVWDLRTGECVQTLSGHTN-SVTSLAWSPdgKRLASGSADGTI 285
WD40 COG2319
WD40 repeat [General function prediction only];
380-666 2.08e-20

WD40 repeat [General function prediction only];


Pssm-ID: 441893 [Multi-domain]  Cd Length: 403  Bit Score: 95.36  E-value: 2.08e-20
                           10        20        30        40        50        60        70        80
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1720396581  380 PDRNFLATGGYDPHIRLWNpLVSKKPVWLMKGHQTSVTHLLVnSRNASILISISRDKNIRVWDLQDYVCLQSFCGklfpl 459
Cdd:COG2319    130 PDGKTLASGSADGTVRLWD-LATGKLLRTLTGHSGAVTSVAF-SPDGKLLASGSDDGTVRLWDLATGKLLRTLTG----- 202

                           90       100       110       120       130       140       150       160
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1720396581  460 GNCPITSTYFYRSSTLICSTYNIGILRGY-LESQGPLKTgrMTTHNTPLCAVLYSKIFKQVVSGCLQGLVSVWDISTGKk 538
Cdd:COG2319    203 HTGAVRSVAFSPDGKLLASGSADGTVRLWdLATGKLLRT--LTGHSGSVRSVAFSPDGRLLASGSADGTVRLWDLATGE- 279

                          170       180       190       200       210       220       230       240
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1720396581  539 rmEIPVSGIQESELTAMALDQSERCLLTGLRDGTMQMWNYNTGECLMTFPNPDRvEISGIVHM--NKVFYTTGWSK--RI 614
Cdd:COG2319    280 --LLRTLTGHSGGVNSVAFSPDGKLLASGSDDGTVRLWDLATGKLLRTLTGHTG-AVRSVAFSpdGKTLASGSDDGtvRL 356

                          250       260       270       280       290
                   ....*....|....*....|....*....|....*....|....*....|...
gi 1720396581  615 TNFTFQKITQALscyhwqSFHTEDILSMD-KYQNQFLGTSSYNGDIVFWNVST 666
Cdd:COG2319    357 WDLATGELLRTL------TGHTGAVTSVAfSPDGRTLASGSADGTVRLWDLAT 403
WD40 COG2319
WD40 repeat [General function prediction only];
372-577 3.68e-19

WD40 repeat [General function prediction only];


Pssm-ID: 441893 [Multi-domain]  Cd Length: 403  Bit Score: 91.51  E-value: 3.68e-19
                           10        20        30        40        50        60        70        80
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1720396581  372 GILCFDYCPDRNFLATGGYDPHIRLWNpLVSKKPVWLMKGHQTSVTHLLVnSRNASILISISRDKNIRVWDLQDYVCLQS 451
Cdd:COG2319    206 AVRSVAFSPDGKLLASGSADGTVRLWD-LATGKLLRTLTGHSGSVRSVAF-SPDGRLLASGSADGTVRLWDLATGELLRT 283

                           90       100       110       120       130       140       150       160
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1720396581  452 FCGklfplGNCPITSTYFYRSSTLICSTYNIGILRGY-LESQGPLKTgrMTTHNTPLCAVLYSKIFKQVVSGCLQGLVSV 530
Cdd:COG2319    284 LTG-----HSGGVNSVAFSPDGKLLASGSDDGTVRLWdLATGKLLRT--LTGHTGAVRSVAFSPDGKTLASGSDDGTVRL 356

                          170       180       190       200
                   ....*....|....*....|....*....|....*....|....*..
gi 1720396581  531 WDISTGKkrmEIPVSGIQESELTAMALDQSERCLLTGLRDGTMQMWN 577
Cdd:COG2319    357 WDLATGE---LLRTLTGHTGAVTSVAFSPDGRTLASGSADGTVRLWD 400
WD40 COG2319
WD40 repeat [General function prediction only];
380-679 3.16e-18

WD40 repeat [General function prediction only];


Pssm-ID: 441893 [Multi-domain]  Cd Length: 403  Bit Score: 88.43  E-value: 3.16e-18
                           10        20        30        40        50        60        70        80
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1720396581  380 PDRNFLATGGYDPHIRLWNpLVSKKPVWLMKGHQTSVTHLLVnSRNASILISISRDKNIRVWDLQDYVCLQSFCGklfpl 459
Cdd:COG2319     88 PDGRLLASASADGTVRLWD-LATGLLLRTLTGHTGAVRSVAF-SPDGKTLASGSADGTVRLWDLATGKLLRTLTG----- 160

                           90       100       110       120       130       140       150       160
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1720396581  460 GNCPITSTYFyrS---STLICSTYNIGILRGYLESQGPLKTgrMTTHNTPLCAVLYSKIFKQVVSGCLQGLVSVWDISTG 536
Cdd:COG2319    161 HSGAVTSVAF--SpdgKLLASGSDDGTVRLWDLATGKLLRT--LTGHTGAVRSVAFSPDGKLLASGSADGTVRLWDLATG 236

                          170       180       190       200       210       220       230       240
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1720396581  537 KkrmEIPVSGIQESELTAMALDQSERCLLTGLRDGTMQMWNYNTGECLMTFPNPDRvEISGIV--HMNKVFYTTGWSKRI 614
Cdd:COG2319    237 K---LLRTLTGHSGSVRSVAFSPDGRLLASGSADGTVRLWDLATGELLRTLTGHSG-GVNSVAfsPDGKLLASGSDDGTV 312

                          250       260       270       280       290       300       310
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|.
gi 1720396581  615 tnftfqKI--TQALSCYHWQSFHTEDILSM----DkyqNQFLGTSSYNGDIVFWNVSTSKPILSFNASKSP 679
Cdd:COG2319    313 ------RLwdLATGKLLRTLTGHTGAVRSVafspD---GKTLASGSDDGTVRLWDLATGELLRTLTGHTGA 374
WD40 cd00200
WD40 domain, found in a number of eukaryotic proteins that cover a wide variety of functions ...
 244-532 2.69e-17

WD40 domain, found in a number of eukaryotic proteins that cover a wide variety of functions including adaptor/regulatory modules in signal transduction, pre-mRNA processing and cytoskeleton assembly; typically contains a GH dipeptide 11-24 residues from its N-terminus and the WD dipeptide at its C-terminus and is 40 residues long, hence the name WD40; between GH and WD lies a conserved core; serves as a stable propeller-like platform to which proteins can bind either stably or reversibly; forms a propeller-like structure with several blades where each blade is composed of a four-stranded anti-parallel b-sheet; instances with few detectable copies are hypothesized to form larger structures by dimerization; each WD40 sequence repeat forms the first three strands of one blade and the last strand in the next blade; the last C-terminal WD40 repeat completes the blade structure of the first WD40 repeat to create the closed ring propeller-structure; residues on the top and bottom surface of the propeller are proposed to coordinate interactions with other proteins and/or small ligands; 7 copies of the repeat are present in this alignment.


Pssm-ID: 238121 [Multi-domain]  Cd Length: 289  Bit Score: 83.92  E-value: 2.69e-17
                           10        20        30        40        50        60        70        80
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1720396581  244 DSCVMVMDYWTDCHKAVFCygdtkgNATIFISDnVAGGLFNPQILprASKWDHwfTVSLKKLlnEKAPLYRSFRMkglHP 323
Cdd:cd00200     30 DGTIKVWDLETGELLRTLK------GHTGPVRD-VAASADGTYLA--SGSSDK--TIRLWDL--ETGECVRTLTG---HT 93

                           90       100       110       120       130       140       150       160
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1720396581  324 NWCQQVKYIPQLNVVASCSA--------VEKSSLVlIILPGKEpekprlsslnlrKGILCFDYCPDRNFLATGGYDPHIR 395
Cdd:cd00200     94 SYVSSVAFSPDGRILSSSSRdktikvwdVETGKCL-TTLRGHT------------DWVNSVAFSPDGTFVASSSQDGTIK 160

                          170       180       190       200       210       220       230       240
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1720396581  396 LWNpLVSKKPVWLMKGHQTSVTHLLVNSRNASILISiSRDKNIRVWDLQDYVCLQSFCGKlfplgNCPITSTYFYRSSTL 475
Cdd:cd00200    161 LWD-LRTGKCVATLTGHTGEVNSVAFSPDGEKLLSS-SSDGTIKLWDLSTGKCLGTLRGH-----ENGVNSVAFSPDGYL 233

                          250       260       270       280       290
                   ....*....|....*....|....*....|....*....|....*....|....*...
gi 1720396581  476 ICSTYNIGILRGY-LESQGPLKTGRMttHNTPLCAVLYSKIFKQVVSGCLQGLVSVWD 532
Cdd:cd00200    234 LASGSEDGTIRVWdLRTGECVQTLSG--HTNSVTSLAWSPDGKRLASGSADGTIRIWD 289
WD40 cd00200
WD40 domain, found in a number of eukaryotic proteins that cover a wide variety of functions ...
 410-679 2.53e-13

WD40 domain, found in a number of eukaryotic proteins that cover a wide variety of functions including adaptor/regulatory modules in signal transduction, pre-mRNA processing and cytoskeleton assembly; typically contains a GH dipeptide 11-24 residues from its N-terminus and the WD dipeptide at its C-terminus and is 40 residues long, hence the name WD40; between GH and WD lies a conserved core; serves as a stable propeller-like platform to which proteins can bind either stably or reversibly; forms a propeller-like structure with several blades where each blade is composed of a four-stranded anti-parallel b-sheet; instances with few detectable copies are hypothesized to form larger structures by dimerization; each WD40 sequence repeat forms the first three strands of one blade and the last strand in the next blade; the last C-terminal WD40 repeat completes the blade structure of the first WD40 repeat to create the closed ring propeller-structure; residues on the top and bottom surface of the propeller are proposed to coordinate interactions with other proteins and/or small ligands; 7 copies of the repeat are present in this alignment.


Pssm-ID: 238121 [Multi-domain]  Cd Length: 289  Bit Score: 71.98  E-value: 2.53e-13
                           10        20        30        40        50        60        70        80
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1720396581  410 KGHQTSVTHLLVNSRNaSILISISRDKNIRVWDLQDYVCLQSFCGklfplgncpitstyfyrsstlicstynigilrgyl 489
Cdd:cd00200      6 KGHTGGVTCVAFSPDG-KLLATGSGDGTIKVWDLETGELLRTLKG----------------------------------- 49

                           90       100       110       120       130       140       150       160
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1720396581  490 esqgplktgrmttHNTPLCAVLYSKIFKQVVSGCLQGLVSVWDISTGKKRMEIpvSGiQESELTAMALDQSERCLLTGLR 569
Cdd:cd00200     50 -------------HTGPVRDVAASADGTYLASGSSDKTIRLWDLETGECVRTL--TG-HTSYVSSVAFSPDGRILSSSSR 113

                          170       180       190       200       210       220       230       240
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1720396581  570 DGTMQMWNYNTGECLMTFPNPDRVEISGIVHMNKVFYTTGWSK---RITNFTFQKITQALscyhwqSFHTEDILSMDKY- 645
Cdd:cd00200    114 DKTIKVWDVETGKCLTTLRGHTDWVNSVAFSPDGTFVASSSQDgtiKLWDLRTGKCVATL------TGHTGEVNSVAFSp 187

                          250       260       270
                   ....*....|....*....|....*....|....
gi 1720396581  646 QNQFLGTSSYNGDIVFWNVSTSKPILSFNASKSP 679
Cdd:cd00200    188 DGEKLLSSSSDGTIKLWDLSTGKCLGTLRGHENG 221
WD40 COG2319
WD40 repeat [General function prediction only];
372-535 5.34e-13

WD40 repeat [General function prediction only];


Pssm-ID: 441893 [Multi-domain]  Cd Length: 403  Bit Score: 72.64  E-value: 5.34e-13
                           10        20        30        40        50        60        70        80
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1720396581  372 GILCFDYCPDRNFLATGGYDPHIRLWNpLVSKKPVWLMKGHQTSVTHLLVnSRNASILISISRDKNIRVWDLQDYVCLQS 451
Cdd:COG2319    248 SVRSVAFSPDGRLLASGSADGTVRLWD-LATGELLRTLTGHSGGVNSVAF-SPDGKLLASGSDDGTVRLWDLATGKLLRT 325

                           90       100       110       120       130       140       150       160
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1720396581  452 FCGKlfplgNCPITSTYFYRSSTLICSTYNIGILRGY-LESQGPLKTgrMTTHNTPLCAVLYSKIFKQVVSGCLQGLVSV 530
Cdd:COG2319    326 LTGH-----TGAVRSVAFSPDGKTLASGSDDGTVRLWdLATGELLRT--LTGHTGAVTSVAFSPDGRTLASGSADGTVRL 398


                   ....*
gi 1720396581  531 WDIST 535
Cdd:COG2319    399 WDLAT 403
WD40 COG2319
WD40 repeat [General function prediction only];
423-674 7.79e-08

WD40 repeat [General function prediction only];


Pssm-ID: 441893 [Multi-domain]  Cd Length: 403  Bit Score: 56.07  E-value: 7.79e-08
                           10        20        30        40        50        60        70        80
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1720396581  423 SRNASILISISRDKNIRVWDLQDYVCLQSFCGKLFPLgncpITSTYFYRSSTLICSTYNIGILRGYLESQGPLKTGrmTT 502
Cdd:COG2319      3 SADGAALAAASADLALALLAAALGALLLLLLGLAAAV----ASLAASPDGARLAAGAGDLTLLLLDAAAGALLATL--LG 76

                           90       100       110       120       130       140       150       160
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1720396581  503 HNTPLCAVLYSKIFKQVVSGCLQGLVSVWDISTGKkrmEIPVSGIQESELTAMALDQSERCLLTGLRDGTMQMWNYNTGE 582
Cdd:COG2319     77 HTAAVLSVAFSPDGRLLASASADGTVRLWDLATGL---LLRTLTGHTGAVRSVAFSPDGKTLASGSADGTVRLWDLATGK 153

                          170       180       190       200       210       220       230       240
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1720396581  583 CLMTFPNPDR----VEIS--GivhmnKVFYTTGWSK--RITNFTFQKITQALSCyhwqsfHTEDILSM----DkyqNQFL 650
Cdd:COG2319    154 LLRTLTGHSGavtsVAFSpdG-----KLLASGSDDGtvRLWDLATGKLLRTLTG------HTGAVRSVafspD---GKLL 219

                          250       260
                   ....*....|....*....|....
gi 1720396581  651 GTSSYNGDIVFWNVSTSKPILSFN 674
Cdd:COG2319    220 ASGSADGTVRLWDLATGKLLRTLT 243
WD40 smart00320
WD40 repeats; Note that these repeats are permuted with respect to the structural repeats ...
 402-442 6.17e-04

WD40 repeats; Note that these repeats are permuted with respect to the structural repeats (blades) of the beta propeller domain.


Pssm-ID: 197651 [Multi-domain]  Cd Length: 40  Bit Score: 38.45  E-value: 6.17e-04
                            10        20        30        40
                    ....*....|....*....|....*....|....*....|.
gi 1720396581   402 SKKPVWLMKGHQTSVTHLLVNSrNASILISISRDKNIRVWD 442
Cdd:smart00320    1 SGELLKTLKGHTGPVTSVAFSP-DGKYLASGSDDGTIKLWD 40
EF-hand_8 pfam13833
EF-hand domain pair;
67-119 1.50e-03

EF-hand domain pair;


Pssm-ID: 404678 [Multi-domain]  Cd Length: 54  Bit Score: 37.68  E-value: 1.50e-03
                           10        20        30        40        50
                   ....*....|....*....|....*....|....*....|....*....|....
gi 1720396581   67 ENGALKKEGFIRIMK-GVLSSMSEEMLELLFLKVDSDCNGFVTWQKYVDYMMRE 119
Cdd:pfam13833    1 EKGVITREELKRALAlLGLKDLSEDEVDILFREFDTDGDGYISFDEFCVLLERR 54
WD40 smart00320
WD40 repeats; Note that these repeats are permuted with respect to the structural repeats ...
 370-398 2.60e-03

WD40 repeats; Note that these repeats are permuted with respect to the structural repeats (blades) of the beta propeller domain.


Pssm-ID: 197651 [Multi-domain]  Cd Length: 40  Bit Score: 36.91  E-value: 2.60e-03
                            10        20
                    ....*....|....*....|....*....
gi 1720396581   370 RKGILCFDYCPDRNFLATGGYDPHIRLWN 398
Cdd:smart00320   12 TGPVTSVAFSPDGKYLASGSDDGTIKLWD 40
WD40 pfam00400
WD domain, G-beta repeat;
370-398 4.95e-03

WD domain, G-beta repeat;


Pssm-ID: 459801 [Multi-domain]  Cd Length: 39  Bit Score: 35.78  E-value: 4.95e-03
                           10        20
                   ....*....|....*....|....*....
gi 1720396581  370 RKGILCFDYCPDRNFLATGGYDPHIRLWN 398
Cdd:pfam00400   11 TGSVTSLAFSPDGKLLASGSDDGTVKVWD 39
KLF3_N cd21577
N-terminal domain of Kruppel-like factor 3; Kruppel-like factor 3 (KLF3; also called ...
 1173-1231 5.52e-03

N-terminal domain of Kruppel-like factor 3; Kruppel-like factor 3 (KLF3; also called Krueppel-like factor 3 and originally called Basic Kruppel-like Factor/BKLF), was the third member of the KLF family of zinc finger transcription factors to be discovered. KLF3 possesses a wide range of biological impacts on regulating apoptosis, differentiation, and proliferation in various tissues during the entire progression process. It has been proposed as a tumor suppressor in colorectal cancer. It appears to function predominantly as a repressor of transcription, turning genes off by recruiting the C-terminal Binding Protein co-repressors CtBP1 and CtBP2. CtBP docks onto a short motif (residues 61-65) in the N-terminus of KLF3, through the Proline-X-Aspartate-Leucine-Serine (PXDLS) motif. CtBP in turn recruits histone modifying enzymes to alter chromatin and repress gene expression. KLF3 belongs to a family of proteins, called the Specificity Protein (SP)/KLF family, characterized by a C-terminal DNA-binding domain of 81 amino acids consisting of three Kruppel-like C2H2 zinc fingers. These factors bind to a loose consensus motif, namely NNRCRCCYY (where N is any nucleotide; R is A/G, and Y is C/T), such as the recurring motifs in GC and GT boxes (5'-GGGGCGGGG-3' and 5-GGTGTGGGG-3') that are present in promoters and more distal regulatory elements of mammalian genes. Members of the KLF family can act as activators or repressors of transcription depending on cell and promoter context. KLFs regulate various cellular functions, such as proliferation, differentiation, and apoptosis, as well as the development and homeostasis of several types of tissue. In addition to the C-terminal DNA-binding domain, each KLF also has a unique N-terminal activation/repression domain that confers specificity and allows it to bind specifically to a certain partner, leading to distinct activities in vivo. This model represents the N-terminal domain of KLF3.


Pssm-ID: 410554 [Multi-domain]  Cd Length: 214  Bit Score: 39.64  E-value: 5.52e-03
                           10        20        30        40        50        60
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*.
gi 1720396581 1173 IPTDMVAGSPAATPS-------SLSMVSVGQSPSNWSTSLKPLSSTSFLRPSSASPAHSGSSTPPH 1231
Cdd:cd21577      3 VKTDMETSFYSPSHSqlepvdlSLSKRSSPPSSSSSSSSSSSSSSSPSSRASPPSPYSKSSPPSPP 68
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
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