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Conserved domains on  [gi|568936126|ref|XP_006535241|]
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bifunctional methylenetetrahydrofolate dehydrogenase/cyclohydrolase 2, mitochondrial isoform X5 [Mus musculus]

Protein Classification

bifunctional 5,10-methylenetetrahydrofolate dehydrogenase/5,10-methenyltetrahydrofolate cyclohydrolase( domain architecture ID 11415140)

bifunctional methylenetetrahydrofolate dehydrogenase/methenyltetrahydrofolate cyclohydrolase reversibly catalyzes oxidation of 5,10-methylene-THF to 5,10-methenyl-THF and hydrolysis of 5,10-methenyl-THF to 10-formyl-THF

Graphical summary

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List of domain hits

 Name Accession Description Interval E-value
FolD COG0190
5,10-methylene-tetrahydrofolate dehydrogenase/Methenyl tetrahydrofolate cyclohydrolase ...
 70-278 1.56e-97

5,10-methylene-tetrahydrofolate dehydrogenase/Methenyl tetrahydrofolate cyclohydrolase [Coenzyme transport and metabolism];


:

Pssm-ID: 439960 [Multi-domain]  Cd Length: 285  Bit Score: 287.68  E-value: 1.56e-97
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 568936126  70 AVIISGTNMAKQIQKEIQQGVESWIALGnRRPHLSIILVGDNPASHTYVRNKIKAASAVGICSELIIKPKNVSQEELLDI 149
Cdd:COG0190    3 AQILDGKAVAAEIREELKERVAALKAKG-ITPGLAVVLVGDDPASQVYVRNKHKACEEVGIESELIRLPADTTQEELLAL 81

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 568936126 150 TDQLNMDPRVSGILVQLPLPG----------------------------------------------------------- 170
Cdd:COG0190   82 IDELNADPSVHGILVQLPLPKhideeavleaidpekdvdgfhpvnlgrlvlgepgfvpctpagimellerygidlagkha 161

                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 568936126 171 --------------------DATVTIAHRYTPreQLKAHTQLADIIIVAAGIPRLITADMVREGAAVIDVGINYIQDpvt 230
Cdd:COG0190  162 vvvgrsnivgkplallllrrNATVTVCHSRTK--DLAEHTRQADILVAAVGKPGLITADMVKPGAVVIDVGINRVED--- 236

                        250       260       270       280
                 ....*....|....*....|....*....|....*....|....*...
gi 568936126 231 gkTKLVGDVDFEAVKKKASFITPVPGGVGPMTVAMLLKNTLLAAKNIA 278
Cdd:COG0190  237 --GKLVGDVDFESVAEKASAITPVPGGVGPMTIAMLLENTLKAAERQA 282
 
Name Accession Description Interval E-value
FolD COG0190
5,10-methylene-tetrahydrofolate dehydrogenase/Methenyl tetrahydrofolate cyclohydrolase ...
 70-278 1.56e-97

5,10-methylene-tetrahydrofolate dehydrogenase/Methenyl tetrahydrofolate cyclohydrolase [Coenzyme transport and metabolism];


Pssm-ID: 439960 [Multi-domain]  Cd Length: 285  Bit Score: 287.68  E-value: 1.56e-97
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 568936126  70 AVIISGTNMAKQIQKEIQQGVESWIALGnRRPHLSIILVGDNPASHTYVRNKIKAASAVGICSELIIKPKNVSQEELLDI 149
Cdd:COG0190    3 AQILDGKAVAAEIREELKERVAALKAKG-ITPGLAVVLVGDDPASQVYVRNKHKACEEVGIESELIRLPADTTQEELLAL 81

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 568936126 150 TDQLNMDPRVSGILVQLPLPG----------------------------------------------------------- 170
Cdd:COG0190   82 IDELNADPSVHGILVQLPLPKhideeavleaidpekdvdgfhpvnlgrlvlgepgfvpctpagimellerygidlagkha 161

                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 568936126 171 --------------------DATVTIAHRYTPreQLKAHTQLADIIIVAAGIPRLITADMVREGAAVIDVGINYIQDpvt 230
Cdd:COG0190  162 vvvgrsnivgkplallllrrNATVTVCHSRTK--DLAEHTRQADILVAAVGKPGLITADMVKPGAVVIDVGINRVED--- 236

                        250       260       270       280
                 ....*....|....*....|....*....|....*....|....*...
gi 568936126 231 gkTKLVGDVDFEAVKKKASFITPVPGGVGPMTVAMLLKNTLLAAKNIA 278
Cdd:COG0190  237 --GKLVGDVDFESVAEKASAITPVPGGVGPMTIAMLLENTLKAAERQA 282
PRK14190 PRK14190
bifunctional 5,10-methylene-tetrahydrofolate dehydrogenase/ 5,10-methylene-tetrahydrofolate ...
 70-275 5.74e-78

bifunctional 5,10-methylene-tetrahydrofolate dehydrogenase/ 5,10-methylene-tetrahydrofolate cyclohydrolase; Provisional


Pssm-ID: 184560 [Multi-domain]  Cd Length: 284  Bit Score: 237.99  E-value: 5.74e-78
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 568936126  70 AVIISGTNMAKQIQKEIQQGVEswiALGNRR--PHLSIILVGDNPASHTYVRNKIKAASAVGICSELIIKPKNVSQEELL 147
Cdd:PRK14190   3 AVIIDGKEVAKEKREQLKEEVV---KLKEQGivPGLAVILVGDDPASHSYVRGKKKAAEKVGIYSELYEFPADITEEELL 79

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 568936126 148 DITDQLNMDPRVSGILVQLPLPG--------------------------------------------------------- 170
Cdd:PRK14190  80 ALIDRLNADPRINGILVQLPLPKhidekavierispekdvdgfhpinvgrmmlgqdtflpctphgilellkeynidisgk 159

                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 568936126 171 ----------------------DATVTIAHRYTPreQLKAHTQLADIIIVAAGIPRLITADMVREGAAVIDVGINYIQDp 228
Cdd:PRK14190 160 hvvvvgrsnivgkpvgqlllneNATVTYCHSKTK--NLAELTKQADILIVAVGKPKLITADMVKEGAVVIDVGVNRLEN- 236

                        250       260       270       280
                 ....*....|....*....|....*....|....*....|....*..
gi 568936126 229 vtgkTKLVGDVDFEAVKKKASFITPVPGGVGPMTVAMLLKNTLLAAK 275
Cdd:PRK14190 237 ----GKLCGDVDFDNVKEKASYITPVPGGVGPMTITMLMHNTVELAK 279
NAD_bind_m-THF_DH_Cyclohyd cd01080
NADP binding domain of methylene-tetrahydrofolate dehydrogenase/cyclohydrolase; NADP binding ...
 163-275 1.39e-56

NADP binding domain of methylene-tetrahydrofolate dehydrogenase/cyclohydrolase; NADP binding domain of the Methylene-Tetrahydrofolate Dehydrogenase/cyclohydrolase (m-THF DH/cyclohydrolase) bifunctional enzyme. Tetrahydrofolate is a versatile carrier of activated one-carbon units. The major one-carbon folate donors are N-5 methyltetrahydrofolate, N5,N10-m-THF, and N10-formayltetrahydrofolate. The oxidation of metabolic intermediate m-THF to m-THF requires the enzyme m-THF DH. In addition, most DHs also have an associated cyclohydrolase activity which catalyzes its hydrolysis to N10-formyltetrahydrofolate. m-THF DH is typically found as part of a multifunctional protein in eukaryotes. NADP-dependent m-THF DH in mammals, birds and yeast are components of a trifunctional enzyme with DH, cyclohydrolase, and synthetase activities. Certain eukaryotic cells also contain homodimeric bifunctional DH/cyclodrolase form. In bacteria, monofucntional DH, as well as bifunctional m-THF m-THF DHm-THF DHDH/cyclodrolase are found. In addition, yeast (S. cerevisiae) also express an monofunctional DH. This family contains the bifunctional DH/cyclohydrolase. M-THF DH, like other amino acid DH-like NAD(P)-binding domains, is a member of the Rossmann fold superfamily which includes glutamate, leucine, and phenylalanine DHs, m-THF DH, methylene-tetrahydromethanopterin DH, m-THF DH/cyclohydrolase, Shikimate DH-like proteins, malate oxidoreductases, and glutamyl tRNA reductase. Amino acid DHs catalyze the deamination of amino acids to keto acids with NAD(P)+ as a cofactor. The NAD(P)-binding Rossmann fold superfamily includes a wide variety of protein families including NAD(P)- binding domains of alcohol DHs, tyrosine-dependent oxidoreductases, glyceraldehyde-3-phosphate DH, lactate/malate DHs, formate/glycerate DHs, siroheme synthases, 6-phosphogluconate DH, amino acid DHs, repressor rex, NAD-binding potassium channel domain, CoA-binding, and ornithine cyclodeaminase-like domains.


Pssm-ID: 133448  Cd Length: 168  Bit Score: 179.29  E-value: 1.39e-56
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 568936126 163 LVQLPLPGDATVTIAHRYTPreQLKAHTQLADIIIVAAGIPRLITADMVREGAAVIDVGINYIQDPvtGKTKLVGDVDFE 242
Cdd:cd01080   60 LAALLLNRNATVTVCHSKTK--NLKEHTKQADIVIVAVGKPGLVKGDMVKPGAVVIDVGINRVPDK--SGGKLVGDVDFE 135

                         90       100       110
                 ....*....|....*....|....*....|...
gi 568936126 243 AVKKKASFITPVPGGVGPMTVAMLLKNTLLAAK 275
Cdd:cd01080  136 SAKEKASAITPVPGGVGPMTVAMLMKNTVEAAK 168
THF_DHG_CYH_C pfam02882
Tetrahydrofolate dehydrogenase/cyclohydrolase, NAD(P)-binding domain;
171-275 4.61e-56

Tetrahydrofolate dehydrogenase/cyclohydrolase, NAD(P)-binding domain;


Pssm-ID: 427036  Cd Length: 160  Bit Score: 177.66  E-value: 4.61e-56
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 568936126  171 DATVTIAHRYTPreQLKAHTQLADIIIVAAGIPRLITADMVREGAAVIDVGINYIqdpvtGKTKLVGDVDFEAVKKKASF 250
Cdd:pfam02882  60 NATVTVCHSKTK--DLAEITREADIVVVAVGKPELIKADWIKPGAVVIDVGINRV-----GNGKLVGDVDFENVKEKASA 132

                          90       100
                  ....*....|....*....|....*
gi 568936126  251 ITPVPGGVGPMTVAMLLKNTLLAAK 275
Cdd:pfam02882 133 ITPVPGGVGPMTVAMLLQNTVEAAK 157
AlaDh_PNT_C smart01002
Alanine dehydrogenase/PNT, C-terminal domain; Alanine dehydrogenase catalyzes the ...
 183-222 8.45e-03

Alanine dehydrogenase/PNT, C-terminal domain; Alanine dehydrogenase catalyzes the NAD-dependent reversible reductive amination of pyruvate into alanine.


Pssm-ID: 214966 [Multi-domain]  Cd Length: 149  Bit Score: 35.95  E-value: 8.45e-03
                           10        20        30        40
                   ....*....|....*....|....*....|....*....|....*...
gi 568936126   183 REQLKAHTQLADIIIVAAGI-----PRLITADMV---REGAAVIDVGI 222
Cdd:smart01002  74 AELLEEAVKEADLVIGAVLIpgakaPKLVTREMVksmKPGSVIVDVAA 121
 
Name Accession Description Interval E-value
FolD COG0190
5,10-methylene-tetrahydrofolate dehydrogenase/Methenyl tetrahydrofolate cyclohydrolase ...
 70-278 1.56e-97

5,10-methylene-tetrahydrofolate dehydrogenase/Methenyl tetrahydrofolate cyclohydrolase [Coenzyme transport and metabolism];


Pssm-ID: 439960 [Multi-domain]  Cd Length: 285  Bit Score: 287.68  E-value: 1.56e-97
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 568936126  70 AVIISGTNMAKQIQKEIQQGVESWIALGnRRPHLSIILVGDNPASHTYVRNKIKAASAVGICSELIIKPKNVSQEELLDI 149
Cdd:COG0190    3 AQILDGKAVAAEIREELKERVAALKAKG-ITPGLAVVLVGDDPASQVYVRNKHKACEEVGIESELIRLPADTTQEELLAL 81

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 568936126 150 TDQLNMDPRVSGILVQLPLPG----------------------------------------------------------- 170
Cdd:COG0190   82 IDELNADPSVHGILVQLPLPKhideeavleaidpekdvdgfhpvnlgrlvlgepgfvpctpagimellerygidlagkha 161

                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 568936126 171 --------------------DATVTIAHRYTPreQLKAHTQLADIIIVAAGIPRLITADMVREGAAVIDVGINYIQDpvt 230
Cdd:COG0190  162 vvvgrsnivgkplallllrrNATVTVCHSRTK--DLAEHTRQADILVAAVGKPGLITADMVKPGAVVIDVGINRVED--- 236

                        250       260       270       280
                 ....*....|....*....|....*....|....*....|....*...
gi 568936126 231 gkTKLVGDVDFEAVKKKASFITPVPGGVGPMTVAMLLKNTLLAAKNIA 278
Cdd:COG0190  237 --GKLVGDVDFESVAEKASAITPVPGGVGPMTIAMLLENTLKAAERQA 282
PRK14190 PRK14190
bifunctional 5,10-methylene-tetrahydrofolate dehydrogenase/ 5,10-methylene-tetrahydrofolate ...
 70-275 5.74e-78

bifunctional 5,10-methylene-tetrahydrofolate dehydrogenase/ 5,10-methylene-tetrahydrofolate cyclohydrolase; Provisional


Pssm-ID: 184560 [Multi-domain]  Cd Length: 284  Bit Score: 237.99  E-value: 5.74e-78
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 568936126  70 AVIISGTNMAKQIQKEIQQGVEswiALGNRR--PHLSIILVGDNPASHTYVRNKIKAASAVGICSELIIKPKNVSQEELL 147
Cdd:PRK14190   3 AVIIDGKEVAKEKREQLKEEVV---KLKEQGivPGLAVILVGDDPASHSYVRGKKKAAEKVGIYSELYEFPADITEEELL 79

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 568936126 148 DITDQLNMDPRVSGILVQLPLPG--------------------------------------------------------- 170
Cdd:PRK14190  80 ALIDRLNADPRINGILVQLPLPKhidekavierispekdvdgfhpinvgrmmlgqdtflpctphgilellkeynidisgk 159

                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 568936126 171 ----------------------DATVTIAHRYTPreQLKAHTQLADIIIVAAGIPRLITADMVREGAAVIDVGINYIQDp 228
Cdd:PRK14190 160 hvvvvgrsnivgkpvgqlllneNATVTYCHSKTK--NLAELTKQADILIVAVGKPKLITADMVKEGAVVIDVGVNRLEN- 236

                        250       260       270       280
                 ....*....|....*....|....*....|....*....|....*..
gi 568936126 229 vtgkTKLVGDVDFEAVKKKASFITPVPGGVGPMTVAMLLKNTLLAAK 275
Cdd:PRK14190 237 ----GKLCGDVDFDNVKEKASYITPVPGGVGPMTITMLMHNTVELAK 279
PRK14186 PRK14186
bifunctional methylenetetrahydrofolate dehydrogenase/methenyltetrahydrofolate cyclohydrolase ...
 70-273 3.71e-74

bifunctional methylenetetrahydrofolate dehydrogenase/methenyltetrahydrofolate cyclohydrolase FolD;


Pssm-ID: 237636 [Multi-domain]  Cd Length: 297  Bit Score: 228.79  E-value: 3.71e-74
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 568936126  70 AVIISGTNMAKQIQKEIQQGVESWIALGNRRPHLSIILVGDNPASHTYVRNKIKAASAVGICSELIIKPKNVSQEELLDI 149
Cdd:PRK14186   2 ALILDGKALAAEIEQRLQAQIESNLPKAGRPPGLAVLRVGDDPASAVYVRNKEKACARVGIASFGKHLPADTSQAEVEAL 81

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 568936126 150 TDQLNMDPRVSGILVQLPLP------------------------------------------------------------ 169
Cdd:PRK14186  82 IAQLNQDERVDGILLQLPLPkhldevpllhaidpdkdadglhplnlgrlvkgepglrsctpagvmrllrsqqidiagkka 161

                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 568936126 170 -------------------GDATVTIAHRYTPreQLKAHTQLADIIIVAAGIPRLITADMVREGAAVIDVGINYIQDPvT 230
Cdd:PRK14186 162 vvvgrsilvgkplalmllaANATVTIAHSRTQ--DLASITREADILVAAAGRPNLIGAEMVKPGAVVVDVGIHRLPSS-D 238

                        250       260       270       280
                 ....*....|....*....|....*....|....*....|...
gi 568936126 231 GKTKLVGDVDFEAVKKKASFITPVPGGVGPMTVAMLLKNTLLA 273
Cdd:PRK14186 239 GKTRLCGDVDFEEVEPVAAAITPVPGGVGPMTVTMLLVNTVLS 281
PRK10792 PRK10792
bifunctional methylenetetrahydrofolate dehydrogenase/methenyltetrahydrofolate cyclohydrolase ...
 70-276 8.21e-73

bifunctional methylenetetrahydrofolate dehydrogenase/methenyltetrahydrofolate cyclohydrolase FolD;


Pssm-ID: 236760 [Multi-domain]  Cd Length: 285  Bit Score: 224.80  E-value: 8.21e-73
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 568936126  70 AVIISGTNMAKQIQKEIQQGVESWIALGNRRPHLSIILVGDNPASHTYVRNKIKAASAVGICSELIIKPKNVSQEELLDI 149
Cdd:PRK10792   3 AKIIDGKTIAQQVRSEVAQKVQARVAAGLRAPGLAVVLVGSDPASQVYVASKRKACEEVGFVSRSYDLPETTSEAELLAL 82

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 568936126 150 TDQLNMDPRVSGILVQLPLPGD---------------------------------------------------------- 171
Cdd:PRK10792  83 IDELNADPTIDGILVQLPLPAHidnvkvlerihpdkdvdgfhpynvgrlaqripllrpctprgimtllerygidtyglna 162

                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 568936126 172 ---------------------ATVTIAHRYTPreQLKAHTQLADIIIVAAGIPRLITADMVREGAAVIDVGINYIQDpvt 230
Cdd:PRK10792 163 vvvgasnivgrpmslelllagCTVTVCHRFTK--NLRHHVRNADLLVVAVGKPGFIPGEWIKPGAIVIDVGINRLED--- 237

                        250       260       270       280
                 ....*....|....*....|....*....|....*....|....*.
gi 568936126 231 gkTKLVGDVDFEAVKKKASFITPVPGGVGPMTVAMLLKNTLLAAKN 276
Cdd:PRK10792 238 --GKLVGDVEFETAAERASWITPVPGGVGPMTVATLLENTLQACEE 281
PRK14189 PRK14189
bifunctional methylenetetrahydrofolate dehydrogenase/methenyltetrahydrofolate cyclohydrolase;
70-278 1.20e-64

bifunctional methylenetetrahydrofolate dehydrogenase/methenyltetrahydrofolate cyclohydrolase;


Pssm-ID: 184559 [Multi-domain]  Cd Length: 285  Bit Score: 203.77  E-value: 1.20e-64
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 568936126  70 AVIISGTNMAKQIQKEIQQGVESWIALGNRrPHLSIILVGDNPASHTYVRNKIKAASAVGICSELIIKPKNVSQEELLDI 149
Cdd:PRK14189   3 AQLIDGNALSKQLRAEAAQRAAALTARGHQ-PGLAVILVGDNPASQVYVRNKVKACEDNGFHSLKDRYPADLSEAELLAR 81

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 568936126 150 TDQLNMDPRVSGILVQLPLPG----------------------------------------------------------- 170
Cdd:PRK14189  82 IDELNRDPKIHGILVQLPLPKhidshkvieaiapekdvdgfhvanagalmtgqplfrpctpygvmkmlesigiplrgaha 161

                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 568936126 171 --------------------DATVTIAHRYTPreQLKAHTQLADIIIVAAGIPRLITADMVREGAAVIDVGINYIQDpvt 230
Cdd:PRK14189 162 vvigrsnivgkpmamlllqaGATVTICHSKTR--DLAAHTRQADIVVAAVGKRNVLTADMVKPGATVIDVGMNRDDA--- 236

                        250       260       270       280
                 ....*....|....*....|....*....|....*....|....*...
gi 568936126 231 gkTKLVGDVDFEAVKKKASFITPVPGGVGPMTVAMLLKNTLLAAKNIA 278
Cdd:PRK14189 237 --GKLCGDVDFAGVKEVAGYITPVPGGVGPMTITMLLVNTIEAAERAA 282
PRK14176 PRK14176
bifunctional 5,10-methylene-tetrahydrofolate dehydrogenase/ 5,10-methylene-tetrahydrofolate ...
 68-278 2.15e-63

bifunctional 5,10-methylene-tetrahydrofolate dehydrogenase/ 5,10-methylene-tetrahydrofolate cyclohydrolase; Provisional


Pssm-ID: 184553 [Multi-domain]  Cd Length: 287  Bit Score: 200.80  E-value: 2.15e-63
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 568936126  68 HEAVIISGTNMAKQIQKEIQQGVESWIALGNRRPHLSIILVGDNPASHTYVRNKIKAASAVGICSELIIKPKNVSQEELL 147
Cdd:PRK14176   6 YESRIIDGKALAKKIEAEVRSGVERLKSNRGITPGLATILVGDDPASKMYVRLKHKACERVGIRAEDQFLPADTTQEELL 85

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 568936126 148 DITDQLNMDPRVSGILVQLPLPG--------------------------------------------------------- 170
Cdd:PRK14176  86 ELIDSLNKRKDVHGILLQLPLPKhldpqeameaidpakdadgfhpynmgklmigdeglvpctphgviraleeygvdiegk 165

                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 568936126 171 ----------------------DATVTIAHRYTprEQLKAHTQLADIIIVAAGIPRLITADMVREGAAVIDVGINYIQDp 228
Cdd:PRK14176 166 navivghsnvvgkpmaamllnrNATVSVCHVFT--DDLKKYTLDADILVVATGVKHLIKADMVKEGAVIFDVGITKEED- 242

                        250       260       270       280       290
                 ....*....|....*....|....*....|....*....|....*....|
gi 568936126 229 vtgktKLVGDVDFEAVKKKASFITPVPGGVGPMTVAMLLKNTLLAAKNIA 278
Cdd:PRK14176 243 -----KVYGDVDFENVIKKASLITPVPGGVGPLTIAMLMKHVLMCAEKSL 287
PRK14191 PRK14191
bifunctional methylenetetrahydrofolate dehydrogenase/methenyltetrahydrofolate cyclohydrolase ...
 71-276 8.95e-63

bifunctional methylenetetrahydrofolate dehydrogenase/methenyltetrahydrofolate cyclohydrolase FolD;


Pssm-ID: 172679 [Multi-domain]  Cd Length: 285  Bit Score: 199.22  E-value: 8.95e-63
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 568936126  71 VIISGTNMAKQIQKEIQQGVESWIALGNRRPHLSIILVGDNPASHTYVRNKIKAASAVGICSELIIKPKNVSQEELLDIT 150
Cdd:PRK14191   2 VLLDGKALSYKIEKDLKNKIQILTAQTGKRPKLAVILVGKDPASQTYVNMKIKACERVGMDSDLHTLQENTTEAELLSLI 81

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 568936126 151 DQLNMDPRVSGILVQLPLP------------------------------------------------------------- 169
Cdd:PRK14191  82 KDLNTDQNIDGILVQLPLPrhidtkmvleaidpnkdvdgfhplnigklcsqldgfvpatpmgvmrllkhyhieikgkdvv 161

                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 568936126 170 ------------------GDATVTIAHRYTprEQLKAHTQLADIIIVAAGIPRLITADMVREGAAVIDVGINYIQDpvtg 231
Cdd:PRK14191 162 iigasnivgkplamlmlnAGASVSVCHILT--KDLSFYTQNADIVCVGVGKPDLIKASMVKKGAVVVDIGINRLND---- 235

                        250       260       270       280
                 ....*....|....*....|....*....|....*....|....*
gi 568936126 232 kTKLVGDVDFEAVKKKASFITPVPGGVGPMTVAMLLKNTLLAAKN 276
Cdd:PRK14191 236 -GRLVGDVDFENVAPKASFITPVPGGVGPMTIVSLLENTLIAAEK 279
PRK14188 PRK14188
bifunctional methylenetetrahydrofolate dehydrogenase/methenyltetrahydrofolate cyclohydrolase ...
 69-278 3.01e-62

bifunctional methylenetetrahydrofolate dehydrogenase/methenyltetrahydrofolate cyclohydrolase FolD;


Pssm-ID: 184558 [Multi-domain]  Cd Length: 296  Bit Score: 198.26  E-value: 3.01e-62
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 568936126  69 EAVIISGTNMAKQIQKEIQQGVESWIALGNRRPHLSIILVGDNPASHTYVRNKIKAASAVGICSELIIKPKNVSQEELLD 148
Cdd:PRK14188   1 MATIIDGKAFAADVRATVAAEVARLKAAHGVTPGLAVVLVGEDPASQVYVRSKGKQTKEAGMASFEHKLPADTSQAELLA 80

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 568936126 149 ITDQLNMDPRVSGILVQLPLPG---------------------------------------------------------- 170
Cdd:PRK14188  81 LIARLNADPAIHGILVQLPLPKhldseaviqaidpekdvdglhvvnagrlatgetalvpctplgcmmllrrvhgdlsgln 160

                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 568936126 171 ---------------------DATVTIAHRYTprEQLKAHTQLADIIIVAAGIPRLITADMVREGAAVIDVGINYI--QD 227
Cdd:PRK14188 161 avvigrsnlvgkpmaqlllaaNATVTIAHSRT--RDLPAVCRRADILVAAVGRPEMVKGDWIKPGATVIDVGINRIpaPE 238

                        250       260       270       280       290
                 ....*....|....*....|....*....|....*....|....*....|.
gi 568936126 228 PVTGKTKLVGDVDFEAVKKKASFITPVPGGVGPMTVAMLLKNTLLAAKNIA 278
Cdd:PRK14188 239 KGEGKTRLVGDVAFAEAAEVAGAITPVPGGVGPMTIACLLANTLTAACRAA 289
PRK14183 PRK14183
bifunctional 5,10-methylene-tetrahydrofolate dehydrogenase/ 5,10-methylene-tetrahydrofolate ...
 72-278 6.26e-59

bifunctional 5,10-methylene-tetrahydrofolate dehydrogenase/ 5,10-methylene-tetrahydrofolate cyclohydrolase; Provisional


Pssm-ID: 184555 [Multi-domain]  Cd Length: 281  Bit Score: 189.27  E-value: 6.26e-59
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 568936126  72 IISGTNMAKQIQKEIQQGVESWIALGNRRPHLSIILVGDNPASHTYVRNKIKAASAVGICSELIIKPKNVSQEELLDITD 151
Cdd:PRK14183   3 ILDGKALSDKIKENVKKEVDELKLVKNIVPGLAVILVGDDPASHTYVKMKAKACDRVGIYSITHEMPSTISQKEILETIA 82

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 568936126 152 QLNMDPRVSGILVQLPLP-------------------------------------------------------------- 169
Cdd:PRK14183  83 MMNNNPNIDGILVQLPLPkhidttkileaidpkkdvdgfhpynvgrlvtgldgfvpctplgvmelleeyeidvkgkdvcv 162

                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 568936126 170 -----------------GDATVTIAHRYTprEQLKAHTQLADIIIVAAGIPRLITADMVREGAAVIDVGINYIQDpvtgk 232
Cdd:PRK14183 163 vgasnivgkpmaalllnANATVDICHIFT--KDLKAHTKKADIVIVGVGKPNLITEDMVKEGAIVIDIGINRTED----- 235

                        250       260       270       280
                 ....*....|....*....|....*....|....*....|....*.
gi 568936126 233 TKLVGDVDFEAVKKKASFITPVPGGVGPMTVAMLLKNTLLAAKNIA 278
Cdd:PRK14183 236 GRLVGDVDFENVAKKCSYITPVPGGVGPMTIAMLLSNTLKAAKNRA 281
PRK14179 PRK14179
bifunctional methylenetetrahydrofolate dehydrogenase/methenyltetrahydrofolate cyclohydrolase;
70-275 1.20e-57

bifunctional methylenetetrahydrofolate dehydrogenase/methenyltetrahydrofolate cyclohydrolase;


Pssm-ID: 237634 [Multi-domain]  Cd Length: 284  Bit Score: 186.11  E-value: 1.20e-57
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 568936126  70 AVIISGTNMAKQIQKEIQQGVESWIALGNRRPHLSIILVGDNPASHTYVRNKIKAASAVGICSELIIKPKNVSQEELLDI 149
Cdd:PRK14179   2 TEIIDGKALAQKMQAELAEKVAKLKEEKGIVPGLVVILVGDNPASQVYVRNKERSALAAGFKSEVVRLPETISQEELLDL 81

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 568936126 150 TDQLNMDPRVSGILVQLPLPG----------------------------------------------------------- 170
Cdd:PRK14179  82 IERYNQDPTWHGILVQLPLPKhineekillaidpkkdvdgfhpmntghlwsgrpvmipctpagimemfreynvelegkha 161

                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 568936126 171 --------------------DATVTIAHRYTPreQLKAHTQLADIIIVAAGIPRLITADMVREGAAVIDVGINYIQDpvt 230
Cdd:PRK14179 162 vvigrsnivgkpmaqllldkNATVTLTHSRTR--NLAEVARKADILVVAIGRGHFVTKEFVKEGAVVIDVGMNRDEN--- 236

                        250       260       270       280
                 ....*....|....*....|....*....|....*....|....*
gi 568936126 231 gkTKLVGDVDFEAVKKKASFITPVPGGVGPMTVAMLLKNTLLAAK 275
Cdd:PRK14179 237 --GKLIGDVDFDEVAEVASYITPVPGGVGPMTITMLMEQTYQAAL 279
PRK14174 PRK14174
bifunctional 5,10-methylene-tetrahydrofolate dehydrogenase/ 5,10-methylene-tetrahydrofolate ...
 71-271 2.58e-57

bifunctional 5,10-methylene-tetrahydrofolate dehydrogenase/ 5,10-methylene-tetrahydrofolate cyclohydrolase; Provisional


Pssm-ID: 172662 [Multi-domain]  Cd Length: 295  Bit Score: 185.41  E-value: 2.58e-57
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 568936126  71 VIISGTNMAKQIQKEIQQGVESWIALGNRRPHLSIILVGDNPASHTYVRNKIKAASAVGICSELIIKPKNVSQEELLDIT 150
Cdd:PRK14174   2 LIIDGKKVSLDLKNELKTRVEAYRAKTGKVPGLTVIIVGEDPASQVYVRNKAKSCKEIGMNSTVIELPADTTEEHLLKKI 81

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 568936126 151 DQLNMDPRVSGILVQLPLP------------------------------------------------------------- 169
Cdd:PRK14174  82 EDLNNDPDVHGILVQQPLPkqidefavtlaidpakdvdgfhpenlgrlvmghldkcfvsctpygilellgrynietkgkh 161

                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 568936126 170 ------------------------GDATVTIAHRYTPreQLKAHTQLADIIIVAAGIPRLITADMVREGAAVIDVGINYI 225
Cdd:PRK14174 162 cvvvgrsnivgkpmanlmlqklkeSNCTVTICHSATK--DIPSYTRQADILIAAIGKARFITADMVKPGAVVIDVGINRI 239

                        250       260       270       280
                 ....*....|....*....|....*....|....*....|....*..
gi 568936126 226 QDPVTGK-TKLVGDVDFEAVKKKASFITPVPGGVGPMTVAMLLKNTL 271
Cdd:PRK14174 240 EDPSTKSgYRLVGDVDYEGVSAKASAITPVPGGVGPMTIAMLLKNTL 286
NAD_bind_m-THF_DH_Cyclohyd cd01080
NADP binding domain of methylene-tetrahydrofolate dehydrogenase/cyclohydrolase; NADP binding ...
 163-275 1.39e-56

NADP binding domain of methylene-tetrahydrofolate dehydrogenase/cyclohydrolase; NADP binding domain of the Methylene-Tetrahydrofolate Dehydrogenase/cyclohydrolase (m-THF DH/cyclohydrolase) bifunctional enzyme. Tetrahydrofolate is a versatile carrier of activated one-carbon units. The major one-carbon folate donors are N-5 methyltetrahydrofolate, N5,N10-m-THF, and N10-formayltetrahydrofolate. The oxidation of metabolic intermediate m-THF to m-THF requires the enzyme m-THF DH. In addition, most DHs also have an associated cyclohydrolase activity which catalyzes its hydrolysis to N10-formyltetrahydrofolate. m-THF DH is typically found as part of a multifunctional protein in eukaryotes. NADP-dependent m-THF DH in mammals, birds and yeast are components of a trifunctional enzyme with DH, cyclohydrolase, and synthetase activities. Certain eukaryotic cells also contain homodimeric bifunctional DH/cyclodrolase form. In bacteria, monofucntional DH, as well as bifunctional m-THF m-THF DHm-THF DHDH/cyclodrolase are found. In addition, yeast (S. cerevisiae) also express an monofunctional DH. This family contains the bifunctional DH/cyclohydrolase. M-THF DH, like other amino acid DH-like NAD(P)-binding domains, is a member of the Rossmann fold superfamily which includes glutamate, leucine, and phenylalanine DHs, m-THF DH, methylene-tetrahydromethanopterin DH, m-THF DH/cyclohydrolase, Shikimate DH-like proteins, malate oxidoreductases, and glutamyl tRNA reductase. Amino acid DHs catalyze the deamination of amino acids to keto acids with NAD(P)+ as a cofactor. The NAD(P)-binding Rossmann fold superfamily includes a wide variety of protein families including NAD(P)- binding domains of alcohol DHs, tyrosine-dependent oxidoreductases, glyceraldehyde-3-phosphate DH, lactate/malate DHs, formate/glycerate DHs, siroheme synthases, 6-phosphogluconate DH, amino acid DHs, repressor rex, NAD-binding potassium channel domain, CoA-binding, and ornithine cyclodeaminase-like domains.


Pssm-ID: 133448  Cd Length: 168  Bit Score: 179.29  E-value: 1.39e-56
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 568936126 163 LVQLPLPGDATVTIAHRYTPreQLKAHTQLADIIIVAAGIPRLITADMVREGAAVIDVGINYIQDPvtGKTKLVGDVDFE 242
Cdd:cd01080   60 LAALLLNRNATVTVCHSKTK--NLKEHTKQADIVIVAVGKPGLVKGDMVKPGAVVIDVGINRVPDK--SGGKLVGDVDFE 135

                         90       100       110
                 ....*....|....*....|....*....|...
gi 568936126 243 AVKKKASFITPVPGGVGPMTVAMLLKNTLLAAK 275
Cdd:cd01080  136 SAKEKASAITPVPGGVGPMTVAMLMKNTVEAAK 168
THF_DHG_CYH_C pfam02882
Tetrahydrofolate dehydrogenase/cyclohydrolase, NAD(P)-binding domain;
171-275 4.61e-56

Tetrahydrofolate dehydrogenase/cyclohydrolase, NAD(P)-binding domain;


Pssm-ID: 427036  Cd Length: 160  Bit Score: 177.66  E-value: 4.61e-56
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 568936126  171 DATVTIAHRYTPreQLKAHTQLADIIIVAAGIPRLITADMVREGAAVIDVGINYIqdpvtGKTKLVGDVDFEAVKKKASF 250
Cdd:pfam02882  60 NATVTVCHSKTK--DLAEITREADIVVVAVGKPELIKADWIKPGAVVIDVGINRV-----GNGKLVGDVDFENVKEKASA 132

                          90       100
                  ....*....|....*....|....*
gi 568936126  251 ITPVPGGVGPMTVAMLLKNTLLAAK 275
Cdd:pfam02882 133 ITPVPGGVGPMTVAMLLQNTVEAAK 157
PRK14184 PRK14184
bifunctional 5,10-methylene-tetrahydrofolate dehydrogenase/ 5,10-methylene-tetrahydrofolate ...
 72-275 1.51e-54

bifunctional 5,10-methylene-tetrahydrofolate dehydrogenase/ 5,10-methylene-tetrahydrofolate cyclohydrolase; Provisional


Pssm-ID: 237635 [Multi-domain]  Cd Length: 286  Bit Score: 178.05  E-value: 1.51e-54
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 568936126  72 IISGTNMAKQIQKEIQQGVESWIALGNRRPHLSIILVGDNPASHTYVRNKIKAASAVGICSELIIKPKNVSQEELLDITD 151
Cdd:PRK14184   3 LLDGKATAATIREELKTEVAALTARHGRAPGLAVILVGEDPASQVYVRNKERACEDAGIVSEAFRLPADTTQEELEDLIA 82

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 568936126 152 QLNMDPRVSGILVQLPLP-------------------------------------------------------------- 169
Cdd:PRK14184  83 ELNARPDIDGILLQLPLPkgldsqrclelidpakdvdgfhpenmgrlalglpgfrpctpagvmtlleryglspagkkavv 162

                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 568936126 170 ---------------------GDATVTIAHRYTPreQLKAHTQLADIIIVAAGIPRLITADMVREGAAVIDVGINYIQDp 228
Cdd:PRK14184 163 vgrsnivgkplalmlgapgkfANATVTVCHSRTP--DLAEECREADFLFVAIGRPRFVTADMVKPGAVVVDVGINRTDD- 239

                        250       260       270       280
                 ....*....|....*....|....*....|....*....|....*..
gi 568936126 229 vtgktKLVGDVDFEAVKKKASFITPVPGGVGPMTVAMLLKNTLLAAK 275
Cdd:PRK14184 240 -----GLVGDCDFEGLSDVASAITPVPGGVGPMTIAQLLVNTVQSWK 281
PRK14166 PRK14166
bifunctional 5,10-methylene-tetrahydrofolate dehydrogenase/ 5,10-methylene-tetrahydrofolate ...
 72-276 2.12e-54

bifunctional 5,10-methylene-tetrahydrofolate dehydrogenase/ 5,10-methylene-tetrahydrofolate cyclohydrolase; Provisional


Pssm-ID: 172654 [Multi-domain]  Cd Length: 282  Bit Score: 177.53  E-value: 2.12e-54
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 568936126  72 IISGTNMAKQIQKEIQQGVEsWIALGNRRPHLSIILVGDNPASHTYVRNKIKAASAVGICSELIIKPKNVSQEELLDITD 151
Cdd:PRK14166   3 LLDGKALSAKIKEELKEKNQ-FLKSKGIESCLAVILVGDNPASQTYVKSKAKACEECGIKSLVYHLNENTTQNELLALIN 81

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 568936126 152 QLNMDPRVSGILVQLPLP-------------------------------------------------------------- 169
Cdd:PRK14166  82 TLNHDDSVHGILVQLPLPdhickdlilesiisskdvdgfhpinvgylnlglesgflpctplgvmkllkayeidlegkdav 161

                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 568936126 170 ------------------GDATVTIAHRYTprEQLKAHTQLADIIIVAAGIPRLITADMVREGAAVIDVGINYIQdpvtg 231
Cdd:PRK14166 162 iigasnivgrpmatmllnAGATVSVCHIKT--KDLSLYTRQADLIIVAAGCVNLLRSDMVKEGVIVVDVGINRLE----- 234

                        250       260       270       280
                 ....*....|....*....|....*....|....*....|....*
gi 568936126 232 KTKLVGDVDFEAVKKKASFITPVPGGVGPMTVAMLLKNTLLAAKN 276
Cdd:PRK14166 235 SGKIVGDVDFEEVSKKSSYITPVPGGVGPMTIAMLLENTVKSAKN 279
PRK14185 PRK14185
bifunctional methylenetetrahydrofolate dehydrogenase/methenyltetrahydrofolate cyclohydrolase ...
 72-279 3.99e-52

bifunctional methylenetetrahydrofolate dehydrogenase/methenyltetrahydrofolate cyclohydrolase FolD;


Pssm-ID: 184556 [Multi-domain]  Cd Length: 293  Bit Score: 171.94  E-value: 3.99e-52
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 568936126  72 IISGTNMAKQIQKEIQQGVESWIALGNRRPHLSIILVGDNPASHTYVRNKIKAASAVGICSELIIKPKNVSQEELLDITD 151
Cdd:PRK14185   3 LIDGKAISAQIKQEIAAEVAEIVAKGGKRPHLAAILVGHDGGSETYVANKVKACEECGFKSSLIRYESDVTEEELLAKVR 82

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 568936126 152 QLNMDPRVSGILVQLPLP-------------------------------------------------------------- 169
Cdd:PRK14185  83 ELNQDDDVDGFIVQLPLPkhiseqkvieaidyrkdvdgfhpinvgrmsiglpcfvsatpngilellkryhietsgkkcvv 162

                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 568936126 170 ---------------------GDATVTIAHRYTPreQLKAHTQLADIIIVAAGIPRLITADMVREGAAVIDVGINYIQDP 228
Cdd:PRK14185 163 lgrsnivgkpmaqlmmqkaypGDCTVTVCHSRSK--NLKKECLEADIIIAALGQPEFVKADMVKEGAVVIDVGTTRVPDA 240

                        250       260       270       280       290
                 ....*....|....*....|....*....|....*....|....*....|..
gi 568936126 229 VTGKT-KLVGDVDFEAVKKKASFITPVPGGVGPMTVAMLLKNTLLAAKNIAY 279
Cdd:PRK14185 241 TRKSGfKLTGDVKFDEVAPKCSYITPVPGGVGPMTIVSLMKNTLLAGKKAIY 292
PRK14187 PRK14187
bifunctional methylenetetrahydrofolate dehydrogenase/methenyltetrahydrofolate cyclohydrolase ...
 69-276 2.28e-50

bifunctional methylenetetrahydrofolate dehydrogenase/methenyltetrahydrofolate cyclohydrolase FolD;


Pssm-ID: 172675 [Multi-domain]  Cd Length: 294  Bit Score: 167.70  E-value: 2.28e-50
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 568936126  69 EAVIISGTNMAKQIQKEIQQGVESWIALGNRRPHLSIILVGDNPASHTYVRNKIKAASAVGICSELIIKPKNVSQEELLD 148
Cdd:PRK14187   1 ETNIIDGKKIANDITEILATCIDDLKRQHNLFPCLIVILVGDDPASQLYVRNKQRKAEMLGLRSETILLPSTISESSLIE 80

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 568936126 149 ITDQLNMDPRVSGILVQLPLPG---------------------------------------------------------- 170
Cdd:PRK14187  81 KINELNNDDSVHGILVQLPVPNhidknliintidpekdvdgfhnenvgrlftgqkknclipctpkgclyliktitrnlsg 160

                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 568936126 171 -----------------------DATVTIAHRYTprEQLKAHTQLADIIIVAAGIPRLITADMVREGAAVIDVGINYIQd 227
Cdd:PRK14187 161 sdavvigrsnivgkpmaclllgeNCTVTTVHSAT--RDLADYCSKADILVAAVGIPNFVKYSWIKKGAIVIDVGINSIE- 237

                        250       260       270       280
                 ....*....|....*....|....*....|....*....|....*....
gi 568936126 228 pVTGKTKLVGDVDFEAVKKKASFITPVPGGVGPMTVAMLLKNTLLAAKN 276
Cdd:PRK14187 238 -EGGVKKFVGDVDFAEVKKKASAITPVPGGVGPMTIAFLMVNTVIAACN 285
PLN02516 PLN02516
methylenetetrahydrofolate dehydrogenase (NADP+)
 68-275 2.89e-50

methylenetetrahydrofolate dehydrogenase (NADP+)


Pssm-ID: 178131 [Multi-domain]  Cd Length: 299  Bit Score: 167.38  E-value: 2.89e-50
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 568936126  68 HEAVIISGTNMAKQIQKEIQQGVESWIALGNRRPHLSIILVGDNPASHTYVRNKIKAASAVGICSELIIKPKNVSQEELL 147
Cdd:PLN02516   7 HVAQIIDGKAIAKAIRSEIAEEVAQLSEKHGKVPGLAVVIVGSRKDSQTYVNMKRKACAEVGIKSFDVDLPENISEAELI 86

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 568936126 148 DITDQLNMDPRVSGILVQLPLP---------------------------------------------------------- 169
Cdd:PLN02516  87 SKVHELNANPDVHGILVQLPLPkhineekilneislekdvdgfhplnigklamkgreplflpctpkgclellsrsgipik 166

                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 568936126 170 -----------------------GDATVTIAHRYTPREQlkAHTQLADIIIVAAGIPRLITADMVREGAAVIDVGINYIQ 226
Cdd:PLN02516 167 gkkavvvgrsnivglpvsllllkADATVTVVHSRTPDPE--SIVREADIVIAAAGQAMMIKGDWIKPGAAVIDVGTNAVS 244

                        250       260       270       280       290
                 ....*....|....*....|....*....|....*....|....*....|.
gi 568936126 227 DPvTGKT--KLVGDVDFEAVKKKASFITPVPGGVGPMTVAMLLKNTLLAAK 275
Cdd:PLN02516 245 DP-SKKSgyRLVGDVDFAEVSKVAGWITPVPGGVGPMTVAMLLKNTVDGAK 294
PRK14167 PRK14167
bifunctional 5,10-methylene-tetrahydrofolate dehydrogenase/ 5,10-methylene-tetrahydrofolate ...
 72-274 1.58e-49

bifunctional 5,10-methylene-tetrahydrofolate dehydrogenase/ 5,10-methylene-tetrahydrofolate cyclohydrolase; Provisional


Pssm-ID: 184549 [Multi-domain]  Cd Length: 297  Bit Score: 165.33  E-value: 1.58e-49
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 568936126  72 IISGTNMAKQIQKEIQQGVESwIALGNRRPHLSIILVGDNPASHTYVRNKIKAASAVGICSELIIKPKNVSQEELLDITD 151
Cdd:PRK14167   4 IIDGNAVAAQIRDDLTDAIET-LEDAGVTPGLATVLMSDDPASETYVSMKQRDCEEVGIEAIDVEIDPDAPAEELYDTID 82

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 568936126 152 QLNMDPRVSGILVQLPLP-------------------------------------------------------------- 169
Cdd:PRK14167  83 ELNADEDVHGILVQMPVPdhvddrevlrridpakdvdgfhpenvgrlvagdarfkpctphgiqkllaaagvdtegadvvv 162

                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 568936126 170 ---------------------GDATVTIAHRYTprEQLKAHTQLADIIIVAAGIPRLITADMVREGAAVIDVGINYIQDP 228
Cdd:PRK14167 163 vgrsdivgkpmanlliqkadgGNATVTVCHSRT--DDLAAKTRRADIVVAAAGVPELIDGSMLSEGATVIDVGINRVDAD 240

                        250       260       270       280
                 ....*....|....*....|....*....|....*....|....*.
gi 568936126 229 VTGKTKLVGDVDFEAVKKKASFITPVPGGVGPMTVAMLLKNTLLAA 274
Cdd:PRK14167 241 TEKGYELVGDVEFESAKEKASAITPVPGGVGPMTRAMLLYNTVKAA 286
PRK14171 PRK14171
bifunctional methylenetetrahydrofolate dehydrogenase/methenyltetrahydrofolate cyclohydrolase ...
 72-279 1.30e-47

bifunctional methylenetetrahydrofolate dehydrogenase/methenyltetrahydrofolate cyclohydrolase FolD;


Pssm-ID: 172659 [Multi-domain]  Cd Length: 288  Bit Score: 160.12  E-value: 1.30e-47
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 568936126  72 IISGTNMAKQIQKEIQQGVESWIALGNRRPHLSIILVGDNPASHTYVRNKIKAASAVGICSELIIKPKNVSQEELLDITD 151
Cdd:PRK14171   4 IIDGKALANEILADLKLEIQELKSQTNASPKLAIVLVGDNPASIIYVKNKIKNAHKIGIDTLLVNLSTTIHTNDLISKIN 83

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 568936126 152 QLNMDPRVSGILVQLPLPG------------------------------------------------------------- 170
Cdd:PRK14171  84 ELNLDNEISGIIVQLPLPSsidknkilsavspskdidgfhplnvgylhsgisqgfipctalgclavikkyepnltgknvv 163

                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 568936126 171 -------------------DATVTIAHRYTprEQLKAHTQLADIIIVAAGIPRLITADMVREGAAVIDVGINYIqdpvtG 231
Cdd:PRK14171 164 iigrsnivgkplsalllkeNCSVTICHSKT--HNLSSITSKADIVVAAIGSPLKLTAEYFNPESIVIDVGINRI-----S 236

                        250       260       270       280
                 ....*....|....*....|....*....|....*....|....*...
gi 568936126 232 KTKLVGDVDFEAVKKKASFITPVPGGVGPMTVAMLLKNTLLAAKNIAY 279
Cdd:PRK14171 237 GNKIIGDVDFENVKSKVKYITPVPGGIGPMTIAFLLKNTVKAFKDSLY 284
PRK14173 PRK14173
bifunctional 5,10-methylene-tetrahydrofolate dehydrogenase/ 5,10-methylene-tetrahydrofolate ...
 70-274 2.49e-47

bifunctional 5,10-methylene-tetrahydrofolate dehydrogenase/ 5,10-methylene-tetrahydrofolate cyclohydrolase; Provisional


Pssm-ID: 184551 [Multi-domain]  Cd Length: 287  Bit Score: 159.61  E-value: 2.49e-47
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 568936126  70 AVIISGTNMAKQIQKEIQQGVESWIAlgnrRPHLSIILVGDNPASHTYVRNKIKAASAVGICSELIIKPKNVSQEELLDI 149
Cdd:PRK14173   3 ARELSGPPAAEAVYAELRARLAKLPF----VPHLRVVRLGEDPASVSYVRLKDRQAKALGLRSQVEVLPESTSQEELLEL 78

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 568936126 150 TDQLNMDPRVSGILVQLPLP------------------------------------------------------------ 169
Cdd:PRK14173  79 IARLNADPEVDGILVQLPLPphidfqrvleaidplkdvdgfhplnvgrlwmggealepctpagvvrllkhygiplagkev 158

                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 568936126 170 -------------------GDATVTIAHRYTPreQLKAHTQLADIIIVAAGIPRLITADMVREGAAVIDVGINYIQDPvT 230
Cdd:PRK14173 159 vvvgrsnivgkplaalllrEDATVTLAHSKTQ--DLPAVTRRADVLVVAVGRPHLITPEMVRPGAVVVDVGINRVGGN-G 235

                        250       260       270       280
                 ....*....|....*....|....*....|....*....|....
gi 568936126 231 GKTKLVGDVDFEaVKKKASFITPVPGGVGPMTVAMLLKNTLLAA 274
Cdd:PRK14173 236 GRDILTGDVHPE-VAEVAGALTPVPGGVGPMTVAMLMANTVIAA 278
PRK14193 PRK14193
bifunctional 5,10-methylene-tetrahydrofolate dehydrogenase/ 5,10-methylene-tetrahydrofolate ...
 70-278 5.97e-47

bifunctional 5,10-methylene-tetrahydrofolate dehydrogenase/ 5,10-methylene-tetrahydrofolate cyclohydrolase; Provisional


Pssm-ID: 237637 [Multi-domain]  Cd Length: 284  Bit Score: 158.25  E-value: 5.97e-47
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 568936126  70 AVIISGTNMAKQIQKEIQQGVESWIALGnRRPHLSIILVGDNPASHTYVRNKIKAASAVGICS---ELiikPKNVSQEEL 146
Cdd:PRK14193   3 AIILDGKATADEIKADLAERVAALKEKG-ITPGLGTVLVGDDPGSQAYVRGKHRDCAEVGITSirrDL---PADATQEEL 78

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 568936126 147 LDITDQLNMDPRVSGILVQLPLPG-------------------------------------------------------- 170
Cdd:PRK14193  79 NAVIDELNADPACTGYIVQLPLPKhldenavleridpakdadglhptnlgrlvlnepaplpctprgivhllrrydvelag 158

                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 568936126 171 -------------------------DATVTIAHryTPREQLKAHTQLADIIIVAAGIPRLITADMVREGAAVIDVGINYI 225
Cdd:PRK14193 159 ahvvvigrgvtvgrpigllltrrseNATVTLCH--TGTRDLAAHTRRADIIVAAAGVAHLVTADMVKPGAAVLDVGVSRA 236

                        250       260       270       280       290
                 ....*....|....*....|....*....|....*....|....*....|...
gi 568936126 226 qdpvtGKTKLVGDVDfEAVKKKASFITPVPGGVGPMTVAMLLKNTLLAAKNIA 278
Cdd:PRK14193 237 -----GDGKLVGDVH-PDVWEVAGAVSPNPGGVGPMTRAFLLTNVVERAERRA 283
PRK14180 PRK14180
bifunctional 5,10-methylene-tetrahydrofolate dehydrogenase/ 5,10-methylene-tetrahydrofolate ...
 71-277 8.59e-47

bifunctional 5,10-methylene-tetrahydrofolate dehydrogenase/ 5,10-methylene-tetrahydrofolate cyclohydrolase; Provisional


Pssm-ID: 172668 [Multi-domain]  Cd Length: 282  Bit Score: 157.88  E-value: 8.59e-47
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 568936126  71 VIISGTNMAKQIQKEIQQGVESWIALGNRRPHLSIILVGDNPASHTYVRNKIKAASAVGICSELIIKPKNVSQEELLDIT 150
Cdd:PRK14180   2 ILIDGKSLSKDLKERLATQVQEYKHHTAITPKLVAIIVGNDPASKTYVASKEKACAQVGIDSQVITLPEHTTESELLELI 81

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 568936126 151 DQLNMDPRVSGILVQLPLP------------------------------------------------------------- 169
Cdd:PRK14180  82 DQLNNDSSVHAILVQLPLPahinknnviysikpekdvdgfhptnvgrlqlrdkkclesctpkgimtmlreygiktegaya 161

                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 568936126 170 -------------------GDATVTIAHRYTprEQLKAHTQLADIIIVAAGIPRLITADMVREGAAVIDVGINYIQDpvt 230
Cdd:PRK14180 162 vvvgasnvvgkpvsqlllnAKATVTTCHRFT--TDLKSHTTKADILIVAVGKPNFITADMVKEGAVVIDVGINHVDG--- 236

                        250       260       270       280
                 ....*....|....*....|....*....|....*....|....*..
gi 568936126 231 gktKLVGDVDFEAVKKKASFITPVPGGVGPMTVAMLLKNTLLAAKNI 277
Cdd:PRK14180 237 ---KIVGDVDFAAVKDKVAAITPVPGGVGPMTITELLYNTFQCAQEL 280
PRK14169 PRK14169
bifunctional methylenetetrahydrofolate dehydrogenase/methenyltetrahydrofolate cyclohydrolase ...
 70-275 1.49e-46

bifunctional methylenetetrahydrofolate dehydrogenase/methenyltetrahydrofolate cyclohydrolase FolD;


Pssm-ID: 184550 [Multi-domain]  Cd Length: 282  Bit Score: 157.41  E-value: 1.49e-46
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 568936126  70 AVIISGTNMAKQIQKEIQQGVESwIALGNRRPHLSIILVGDNPASHTYVRNKIKAASAVGICSELIIKPKNVSQEELLDI 149
Cdd:PRK14169   1 ATRLDGRAVSKKILADLKQTVAK-LAQQDVTPTLAVVLVGSDPASEVYVRNKQRRAEDIGVRSLMFRLPEATTQADLLAK 79

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 568936126 150 TDQLNMDPRVSGILVQLPLPG----------------------------------------------------------- 170
Cdd:PRK14169  80 VAELNHDPDVDAILVQLPLPAgldeqavidaidpdkdvdgfspvsvgrlwaneptvvastpygimalldaydidvagkrv 159

                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 568936126 171 --------------------DATVTIAHRYTprEQLKAHTQLADIIIVAAGIPRLITADMVREGAAVIDVGINYIQDpvt 230
Cdd:PRK14169 160 vivgrsnivgrplaglmvnhDATVTIAHSKT--RNLKQLTKEADILVVAVGVPHFIGADAVKPGAVVIDVGISRGAD--- 234

                        250       260       270       280
                 ....*....|....*....|....*....|....*....|....*
gi 568936126 231 gkTKLVGDVDFEAVKKKASFITPVPGGVGPMTVAMLLKNTLLAAK 275
Cdd:PRK14169 235 --GKLLGDVDEAAVAPIASAITPVPGGVGPMTIASLMAQTVTLAK 277
PRK14168 PRK14168
bifunctional methylenetetrahydrofolate dehydrogenase/methenyltetrahydrofolate cyclohydrolase ...
 70-275 1.08e-45

bifunctional methylenetetrahydrofolate dehydrogenase/methenyltetrahydrofolate cyclohydrolase FolD;


Pssm-ID: 237633 [Multi-domain]  Cd Length: 297  Bit Score: 155.42  E-value: 1.08e-45
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 568936126  70 AVIISGTNMAKQIQKEIQQGVESWIALGNRRPHLSIILVGDNPASHTYVRNKIKAASAVGICSELIIKPKNVSQEELLDI 149
Cdd:PRK14168   3 AKIIKGTEIREEILEEIRGEVAELKEKYGKVPGLVTILVGESPASLSYVTLKIKTAHRLGFHEIQDNQSVDITEEELLAL 82

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 568936126 150 TDQLNMDPRVSGILVQLPLP------------------------------------------------------------ 169
Cdd:PRK14168  83 IDKYNNDDSIHGILVQLPLPkhinekkvlnaidpdkdvdgfhpvnvgrlmiggdevkflpctpagiqemlvrsgvetsga 162

                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 568936126 170 -------------------------GDATVTIAHryTPREQLKAHTQLADIIIVAAGIPRLITADMVREGAAVIDVGINY 224
Cdd:PRK14168 163 evvvvgrsnivgkpianmmtqkgpgANATVTIVH--TRSKNLARHCQRADILIVAAGVPNLVKPEWIKPGATVIDVGVNR 240

                        250       260       270       280       290
                 ....*....|....*....|....*....|....*....|....*....|..
gi 568936126 225 I-QDPVTGKTKLVGDVDFEAVKKKASFITPVPGGVGPMTVAMLLKNTLLAAK 275
Cdd:PRK14168 241 VgTNESTGKAILSGDVDFDAVKEIAGKITPVPGGVGPMTIAMLMRNTLKSAK 292
PRK14175 PRK14175
bifunctional methylenetetrahydrofolate dehydrogenase/methenyltetrahydrofolate cyclohydrolase ...
 70-275 1.48e-45

bifunctional methylenetetrahydrofolate dehydrogenase/methenyltetrahydrofolate cyclohydrolase FolD;


Pssm-ID: 184552 [Multi-domain]  Cd Length: 286  Bit Score: 154.69  E-value: 1.48e-45
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 568936126  70 AVIISGTNMAKQIQKEIQQGVESWIALGnRRPHLSIILVGDNPASHTYVRNKIKAASAVGICSELIIKPKNVSQEELLDI 149
Cdd:PRK14175   3 AKILDGKQIAKDYRQGLQDQVEALKEKG-FTPKLSVILVGNDGASQSYVRSKKKAAEKIGMISEIVHLEETATEEEVLNE 81

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 568936126 150 TDQLNMDPRVSGILVQLPLP------------------------------------------------------------ 169
Cdd:PRK14175  82 LNRLNNDDSVSGILVQVPLPkqvseqkileainpekdvdgfhpinigklyideqtfvpctplgimeilkhadidlegkna 161

                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 568936126 170 -------------------GDATVTIAHRYTprEQLKAHTQLADIIIVAAGIPRLITADMVREGAAVIDVGinyiqDPVT 230
Cdd:PRK14175 162 vvigrshivgqpvsklllqKNASVTILHSRS--KDMASYLKDADVIVSAVGKPGLVTKDVVKEGAVIIDVG-----NTPD 234

                        250       260       270       280
                 ....*....|....*....|....*....|....*....|....*
gi 568936126 231 GKTKLVGDVDFEAVKKKASFITPVPGGVGPMTVAMLLKNTLLAAK 275
Cdd:PRK14175 235 ENGKLKGDVDYDAVKEIAGAITPVPGGVGPLTITMVLNNTLLAEK 279
PLN02616 PLN02616
tetrahydrofolate dehydrogenase/cyclohydrolase, putative
 70-277 2.88e-45

tetrahydrofolate dehydrogenase/cyclohydrolase, putative


Pssm-ID: 215332 [Multi-domain]  Cd Length: 364  Bit Score: 156.32  E-value: 2.88e-45
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 568936126  70 AVIISGTNMAKQIQKEIQQGVESWIALGNRRPHLSIILVGDNPASHTYVRNKIKAASAVGICSELIIKPKNVSQEELLDI 149
Cdd:PLN02616  73 AKVIDGKAVAKKIRDEITIEVSRMKESIGVVPGLAVILVGDRKDSATYVRNKKKACDSVGINSFEVRLPEDSTEQEVLKF 152

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 568936126 150 TDQLNMDPRVSGILVQLPLPG----------------------------------------------------------- 170
Cdd:PLN02616 153 ISGFNNDPSVHGILVQLPLPShmdeqnilnavsiekdvdgfhplnigrlamrgreplfvpctpkgciellhrynveikgk 232

                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 568936126 171 ----------------------DATVTIAHRYT--PREQlkahTQLADIIIVAAGIPRLITADMVREGAAVIDVGINYIQ 226
Cdd:PLN02616 233 ravvigrsnivgmpaalllqreDATVSIVHSRTknPEEI----TREADIIISAVGQPNMVRGSWIKPGAVVIDVGINPVE 308

                        250       260       270       280       290
                 ....*....|....*....|....*....|....*....|....*....|..
gi 568936126 227 DPVTGKT-KLVGDVDFEAVKKKASFITPVPGGVGPMTVAMLLKNTLLAAKNI 277
Cdd:PLN02616 309 DASSPRGyRLVGDVCYEEACKVASAVTPVPGGVGPMTIAMLLSNTLTSAKRI 360
PRK14178 PRK14178
bifunctional methylenetetrahydrofolate dehydrogenase/methenyltetrahydrofolate cyclohydrolase ...
 101-276 5.11e-45

bifunctional methylenetetrahydrofolate dehydrogenase/methenyltetrahydrofolate cyclohydrolase FolD;


Pssm-ID: 172666 [Multi-domain]  Cd Length: 279  Bit Score: 153.08  E-value: 5.11e-45
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 568936126 101 PHLSIILVGDNPASHTYVRNKIKAASAVGICSELIIKPKNVSQEELLDITDQLNMDPRVSGILVQLPLPG---------- 170
Cdd:PRK14178  27 PRLATVIVGDDPASQMYVRMKHRACERVGIGSVGIELPGDATTRTVLERIRRLNEDPDINGILVQLPLPKgvdterviaa 106

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 568936126 171 ---------------------------------------------------------------------DATVTIAHRYT 181
Cdd:PRK14178 107 ilpekdvdgfhplnlgrlvsglpgfapctpngimtllheykisiagkravvvgrsidvgrpmaalllnaDATVTICHSKT 186

                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 568936126 182 prEQLKAHTQLADIIIVAAGIPRLITADMVREGAAVIDVGINYIQDpvtgktKLVGDVDFEAVKKKASFITPVPGGVGPM 261
Cdd:PRK14178 187 --ENLKAELRQADILVSAAGKAGFITPDMVKPGATVIDVGINQVNG------KLCGDVDFDAVKEIAGAITPVPGGVGPM 258

                        250
                 ....*....|....*
gi 568936126 262 TVAMLLKNTLLAAKN 276
Cdd:PRK14178 259 TIATLMENTFDAAKM 273
PRK14194 PRK14194
bifunctional methylenetetrahydrofolate dehydrogenase/methenyltetrahydrofolate cyclohydrolase ...
 72-275 8.15e-45

bifunctional methylenetetrahydrofolate dehydrogenase/methenyltetrahydrofolate cyclohydrolase FolD;


Pssm-ID: 172682 [Multi-domain]  Cd Length: 301  Bit Score: 153.46  E-value: 8.15e-45
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 568936126  72 IISGTNMAKQIQKEIQQGVESWIALGnRRPHLSIILVGDNPASHTYVRNKIKAASAVGICSELIIKPKNVSQEELLDITD 151
Cdd:PRK14194   6 LIDGKAAAARVLAQVREDVRTLKAAG-IEPALAVILVGNDPASQVYVRNKILRAEEAGIRSLEHRLPADTSQARLLALIA 84

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 568936126 152 QLNMDPRVSGILVQLPLP-------------------------------------------------------------- 169
Cdd:PRK14194  85 ELNADPSVNGILLQLPLPahidearvlqainplkdvdgfhsenvgglsqgrdvltpctpsgclrlledtcgdltgkhavv 164

                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 568936126 170 -----------------GDATVTIAHRYTprEQLKAHTQLADIIIVAAGIPRLITADMVREGAAVIDVGINYIQDpvTGK 232
Cdd:PRK14194 165 igrsnivgkpmaalllqAHCSVTVVHSRS--TDAKALCRQADIVVAAVGRPRLIDADWLKPGAVVIDVGINRIDD--DGR 240

                        250       260       270       280
                 ....*....|....*....|....*....|....*....|...
gi 568936126 233 TKLVGDVDFEAVKKKASFITPVPGGVGPMTVAMLLKNTLLAAK 275
Cdd:PRK14194 241 SRLVGDVDFDSALPVVSAITPVPGGVGPMTIAFLMKNTVTAAR 283
PRK14170 PRK14170
bifunctional methylenetetrahydrofolate dehydrogenase/methenyltetrahydrofolate cyclohydrolase ...
 72-277 3.22e-44

bifunctional methylenetetrahydrofolate dehydrogenase/methenyltetrahydrofolate cyclohydrolase FolD;


Pssm-ID: 172658 [Multi-domain]  Cd Length: 284  Bit Score: 151.38  E-value: 3.22e-44
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 568936126  72 IISGTNMAKQIQKEIQQGVESWIALGnRRPHLSIILVGDNPASHTYVRNKIKAASAVGICSELIIKPKNVSQEELLDITD 151
Cdd:PRK14170   4 IIDGKKLAKEIQEKVTREVAELVKEG-KKPGLAVVLVGDNQASRTYVRNKQKRTEEAGMKSVLIELPENVTEEKLLSVVE 82

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 568936126 152 QLNMDPRVSGILVQLPLPG------------------------------------------------------------- 170
Cdd:PRK14170  83 ELNEDKTIHGILVQLPLPEhiseekvidtisydkdvdgfhpvnvgnlfigkdsfvpctpagiielikstgtqiegkravv 162

                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 568936126 171 ------------------DATVTIAHRYTprEQLKAHTQLADIIIVAAGIPRLITADMVREGAAVIDVGINYIQDpvtgk 232
Cdd:PRK14170 163 igrsnivgkpvaqlllneNATVTIAHSRT--KDLPQVAKEADILVVATGLAKFVKKDYIKPGAIVIDVGMDRDEN----- 235

                        250       260       270       280
                 ....*....|....*....|....*....|....*....|....*
gi 568936126 233 TKLVGDVDFEAVKKKASFITPVPGGVGPMTVAMLLKNTLLAAKNI 277
Cdd:PRK14170 236 NKLCGDVDFDDVVEEAGFITPVPGGVGPMTITMLLANTLKAAKRI 280
PRK14177 PRK14177
bifunctional methylenetetrahydrofolate dehydrogenase/methenyltetrahydrofolate cyclohydrolase ...
 69-275 3.88e-44

bifunctional methylenetetrahydrofolate dehydrogenase/methenyltetrahydrofolate cyclohydrolase FolD;


Pssm-ID: 172665 [Multi-domain]  Cd Length: 284  Bit Score: 151.28  E-value: 3.88e-44
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 568936126  69 EAVIISGTNMAKQIQKEIQQGVESWIALGNRRPHLSIILVGDNPASHTYVRNKIKAASAVGICSELIIKPKNVSQEELLD 148
Cdd:PRK14177   2 SPILLDGKKLSEKIRNEIRETIEERKTKNKRIPKLATILVGNNPASETYVSMKVKACHKVGMGSEMIRLKEQTTTEELLG 81

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 568936126 149 ITDQLNMDPRVSGILVQLPLPG---------------------------------------------------------- 170
Cdd:PRK14177  82 VIDKLNLDPNVDGILLQHPVPSqideraafdrialekdvdgvttlsfgklsmgvetylpctpygmvlllkeygidvtgkn 161

                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 568936126 171 ---------------------DATVTIAHRYTprEQLKAHTQLADIIIVAAGIPRLITADMVREGAAVIDVGINyiqdpv 229
Cdd:PRK14177 162 avvvgrspilgkpmamlltemNATVTLCHSKT--QNLPSIVRQADIIVGAVGKPEFIKADWISEGAVLLDAGYN------ 233

                        250       260       270       280
                 ....*....|....*....|....*....|....*....|....*.
gi 568936126 230 TGKtklVGDVDFEAVKKKASFITPVPGGVGPMTVAMLLKNTLLAAK 275
Cdd:PRK14177 234 PGN---VGDIEISKAKDKSSFYTPVPGGVGPMTIAVLLLQTLYSFK 276
PRK14172 PRK14172
bifunctional 5,10-methylene-tetrahydrofolate dehydrogenase/ 5,10-methylene-tetrahydrofolate ...
 72-269 2.03e-43

bifunctional 5,10-methylene-tetrahydrofolate dehydrogenase/ 5,10-methylene-tetrahydrofolate cyclohydrolase; Provisional


Pssm-ID: 172660 [Multi-domain]  Cd Length: 278  Bit Score: 149.16  E-value: 2.03e-43
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 568936126  72 IISGTNMAKQIQKEIQQGVESWIALGNRRPHLSIILVGDNPASHTYVRNKIKAASAVGICSELIIKPKNVSQEELLDITD 151
Cdd:PRK14172   4 IINGKEVALKIKEEIKNFVEERKENGLSIPKIASILVGNDGGSIYYMNNQEKVANSLGIDFKKIKLDESISEEDLINEIE 83

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 568936126 152 QLNMDPRVSGILVQLPLPG------------------------------------------------------------- 170
Cdd:PRK14172  84 ELNKDNNVHGIMLQLPLPKhldekkitnkidankdidcltfisvgkfykgekcflpctpnsvitlikslnidiegkevvv 163

                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 568936126 171 ------------------DATVTIAHRYTprEQLKAHTQLADIIIVAAGIPRLITADMVREGAAVIDVGINYIQDpvtgk 232
Cdd:PRK14172 164 igrsnivgkpvaqlllneNATVTICHSKT--KNLKEVCKKADILVVAIGRPKFIDEEYVKEGAIVIDVGTSSVNG----- 236

                        250       260       270
                 ....*....|....*....|....*....|....*..
gi 568936126 233 tKLVGDVDFEAVKKKASFITPVPGGVGPMTVAMLLKN 269
Cdd:PRK14172 237 -KITGDVNFDKVIDKASYITPVPGGVGSLTTTLLIKN 272
THF_DHG_CYH pfam00763
Tetrahydrofolate dehydrogenase/cyclohydrolase, catalytic domain;
73-170 1.02e-42

Tetrahydrofolate dehydrogenase/cyclohydrolase, catalytic domain;


Pssm-ID: 459930 [Multi-domain]  Cd Length: 115  Bit Score: 141.77  E-value: 1.02e-42
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 568936126   73 ISGTNMAKQIQKEIQQGVESWIALGnRRPHLSIILVGDNPASHTYVRNKIKAASAVGICSELIIKPKNVSQEELLDITDQ 152
Cdd:pfam00763   1 IDGKAIAKKIREELKEEVAALKAGG-RKPGLAVILVGDDPASQVYVRNKKKACEEVGIESELIRLPEDTTEEELLALIDK 79

                          90
                  ....*....|....*...
gi 568936126  153 LNMDPRVSGILVQLPLPG 170
Cdd:pfam00763  80 LNADPSVHGILVQLPLPK 97
PLN02897 PLN02897
tetrahydrofolate dehydrogenase/cyclohydrolase, putative
 46-277 2.25e-42

tetrahydrofolate dehydrogenase/cyclohydrolase, putative


Pssm-ID: 178485 [Multi-domain]  Cd Length: 345  Bit Score: 148.18  E-value: 2.25e-42
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 568936126  46 LHLPEVVCLPLACPCTTVCEIRHEAVIISGTNMAKQIQKEIQQGVESWIALGNRRPHLSIILVGDNPASHTYVRNKIKAA 125
Cdd:PLN02897  32 LDLPENWIPYSDPPPPVSFETEQKTVVIDGNVIAEEIRTKIASEVRKMKKAVGKVPGLAVVLVGQQRDSQTYVRNKIKAC 111

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 568936126 126 SAVGICSELIIKPKNVSQEELLDITDQLNMDPRVSGILVQLPLPG----------------------------------- 170
Cdd:PLN02897 112 EETGIKSLLAELPEDCTEGQILSALRKFNEDTSIHGILVQLPLPQhldeskilnmvrlekdvdgfhplnvgnlamrgrep 191

                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 568936126 171 ----------------------------------------------DATVTIAHRYTPR-EQLkahTQLADIIIVAAGIP 203
Cdd:PLN02897 192 lfvsctpkgcvellirsgveiagknavvigrsnivglpmslllqrhDATVSTVHAFTKDpEQI---TRKADIVIAAAGIP 268

                        250       260       270       280       290       300       310
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*
gi 568936126 204 RLITADMVREGAAVIDVGINYIQDPVTG-KTKLVGDVDFEAVKKKASFITPVPGGVGPMTVAMLLKNTLLAAKNI 277
Cdd:PLN02897 269 NLVRGSWLKPGAVVIDVGTTPVEDSSCEfGYRLVGDVCYEEALGVASAITPVPGGVGPMTITMLLCNTLDAAKRI 343
PRK14192 PRK14192
bifunctional methylenetetrahydrofolate dehydrogenase/methenyltetrahydrofolate cyclohydrolase ...
 70-275 1.13e-37

bifunctional methylenetetrahydrofolate dehydrogenase/methenyltetrahydrofolate cyclohydrolase FolD;


Pssm-ID: 184561 [Multi-domain]  Cd Length: 283  Bit Score: 134.20  E-value: 1.13e-37
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 568936126  70 AVIISGTNMAKQIQKEIQQGVESWIALGNRRPHLSIILVGDNPASHTYVRNKIKAASAVGICSELIIKPKNVSQEELLDI 149
Cdd:PRK14192   3 ALVLDGKALAKQIEEELSVRVEALKAKTGRTPILATILVGDDPASATYVRMKGNACRRVGMDSLKVELPQETTTEQLLAK 82

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 568936126 150 TDQLNMDPRVSGILVQLPLP------------------------------------------------------------ 169
Cdd:PRK14192  83 IEELNANPDVHGILLQHPVPaqideracfdaislakdvdgvtclgfgrmamgeaaygsatpagimrllkaynielagkha 162

                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 568936126 170 -------------------GDATVTIAHRYTprEQLKAHTQLADIIIVAAGIPRLITADMVREGAAVIDVGINyiqdPVT 230
Cdd:PRK14192 163 vvvgrsailgkpmammllnANATVTICHSRT--QNLPELVKQADIIVGAVGKPELIKKDWIKQGAVVVDAGFH----PRD 236

                        250       260       270       280
                 ....*....|....*....|....*....|....*....|....*
gi 568936126 231 GKTklVGDVDFEAVKKKASFITPVPGGVGPMTVAMLLKNTLLAAK 275
Cdd:PRK14192 237 GGG--VGDIELQGIEEIASAYTPVPGGVGPMTINTLIRQTVEAAE 279
PRK14182 PRK14182
bifunctional 5,10-methylene-tetrahydrofolate dehydrogenase/ 5,10-methylene-tetrahydrofolate ...
 72-278 3.07e-36

bifunctional 5,10-methylene-tetrahydrofolate dehydrogenase/ 5,10-methylene-tetrahydrofolate cyclohydrolase; Provisional


Pssm-ID: 172670 [Multi-domain]  Cd Length: 282  Bit Score: 130.53  E-value: 3.07e-36
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 568936126  72 IISGTNMAKQIQKEIQQGVESWIALGnRRPHLSIILVGDNPASHTYVRNKIKAASAVGICSELIIKPKNVSQEELLDITD 151
Cdd:PRK14182   3 LIDGKQIAAKVKGEVATEVRALAARG-VQTGLTVVRVGDDPASAIYVRGKRKDCEEVGITSVEHHLPATTTQAELLALIA 81

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 568936126 152 QLNMDPRVSGILVQLPLPGD------------------------------------------------------------ 171
Cdd:PRK14182  82 RLNADPAVHGILVQLPLPKHvderavldaispakdadgfhpfnvgalsigiagvprpctpagvmrmldearvdpkgkral 161

                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 568936126 172 --------------------ATVTIAHRYTprEQLKAHTQLADIIIVAAGIPRLITADMVREGAAVIDVGINYIQDpvtg 231
Cdd:PRK14182 162 vvgrsnivgkpmammllerhATVTIAHSRT--ADLAGEVGRADILVAAIGKAELVKGAWVKEGAVVIDVGMNRLAD---- 235

                        250       260       270       280
                 ....*....|....*....|....*....|....*....|....*..
gi 568936126 232 kTKLVGDVDFEAVKKKASFITPVPGGVGPMTVAMLLKNTLLAAKNIA 278
Cdd:PRK14182 236 -GKLVGDVEFAAAAARASAITPVPGGVGPMTRAMLLVNTVELAKRTA 281
PRK14181 PRK14181
bifunctional methylenetetrahydrofolate dehydrogenase/methenyltetrahydrofolate cyclohydrolase ...
 71-270 1.96e-35

bifunctional methylenetetrahydrofolate dehydrogenase/methenyltetrahydrofolate cyclohydrolase FolD;


Pssm-ID: 172669 [Multi-domain]  Cd Length: 287  Bit Score: 128.44  E-value: 1.96e-35
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 568936126  71 VIISGTNMAKQIQKEIQQGVESwialGNRRPHLSIILVGDNPASHTYVRNKIKAASAVGICSELIIKPKNVSQEELLDIT 150
Cdd:PRK14181   1 MLLKGAPAAEHILATIKENISA----SSTAPGLAVVLIGNDPASEVYVGMKVKKATDLGMVSKAHRLPSDATLSDILKLI 76

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 568936126 151 DQLNMDPRVSGILVQLPLPG------------------------------------------------------------ 170
Cdd:PRK14181  77 HRLNNDPNIHGILVQLPLPKhldaqailqaispdkdvdglhpvnmgklllgetdgfipctpagiiellkyyeiplhgrhv 156

                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 568936126 171 ------------------------DATVTIAHryTPREQLKAHTQLADIIIVAAGIPRLITADMVREGAAVIDVGINYIQ 226
Cdd:PRK14181 157 aivgrsnivgkplaallmqkhpdtNATVTLLH--SQSENLTEILKTADIIIAAIGVPLFIKEEMIAEKAVIVDVGTSRVP 234

                        250       260       270       280
                 ....*....|....*....|....*....|....*....|....
gi 568936126 227 DPVTGKTKLVGDVDFEAVKKKASFITPVPGGVGPMTVAMLLKNT 270
Cdd:PRK14181 235 AANPKGYILVGDVDFNNVVPKCRAITPVPGGVGPMTVAMLMRNT 278
NAD_bind_m-THF_DH_Cyclohyd_like cd05212
NAD(P) binding domain of methylene-tetrahydrofolate dehydrogenase and ...
 172-275 4.72e-13

NAD(P) binding domain of methylene-tetrahydrofolate dehydrogenase and methylene-tetrahydrofolate dehydrogenase/cyclohydrolase; NAD(P) binding domains of methylene-tetrahydrofolate dehydrogenase (m-THF DH) and m-THF DH/cyclohydrolase bifunctional enzymes (m-THF DH/cyclohydrolase). M-THF is a versatile carrier of activated one-carbon units. The major one-carbon folate donors are N-5 methyltetrahydrofolate, N5,N10-m-THF, and N10-formayltetrahydrofolate. The oxidation of metabolic intermediate m-THF to m-THF requires the enzyme m-THF DH. In addition, most DHs also have an associated cyclohydrolase activity which catalyzes its hydrolysis to N10-formyltetrahydrofolate. m-THF DH is typically found as part of a multifunctional protein in eukaryotes. NADP-dependent m-THF DH in mammals, birds and yeast are components of a trifunctional enzyme with DH, cyclohydrolase, and synthetase activities. Certain eukaryotic cells also contain homodimeric bifunctional DH/cyclodrolase form. In bacteria, mono-functional DH, as well as bifunctional DH/cyclodrolase are found. In addition, yeast (S. cerevisiae) also express a monofunctional DH. M-THF DH, like other amino acid DH-like NAD(P)-binding domains, is a member of the Rossmann fold superfamily which includes glutamate, leucine, and phenylalanine DHs, m-THF DH, methylene-tetrahydromethanopterin DH, m-THF DH/cyclohydrolase, Shikimate DH-like proteins, malate oxidoreductases, and glutamyl tRNA reductase. Amino acid DHs catalyze the deamination of amino acids to keto acids with NAD(P)+ as a cofactor. The NAD(P)-binding Rossmann fold superfamily includes a wide variety of protein families including NAD(P)- binding domains of alcohol DHs, tyrosine-dependent oxidoreductases, glyceraldehyde-3-phosphate DH, lactate/malate DHs, formate/glycerate DHs, siroheme synthases, 6-phosphogluconate DH, amino acid DHs, repressor rex, NAD-binding potassium channel domain, CoA-binding, and ornithine cyclodeaminase-like domains. These domains have an alpha-beta-alpha configuration. NAD binding involves numerous hydrogen and van der Waals contacts.


Pssm-ID: 133451  Cd Length: 140  Bit Score: 64.84  E-value: 4.72e-13
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 568936126 172 ATVTIAHRYTPREQLKAHTqlADIIIVAAGIPRLITADMVREGAAVIDVGINYIQDpvtgktklvgdvdfEAVKKKASFI 251
Cdd:cd05212   53 ATVYSCDWKTIQLQSKVHD--ADVVVVGSPKPEKVPTEWIKPGATVINCSPTKLSG--------------DDVKESASLY 116

                         90       100
                 ....*....|....*....|....
gi 568936126 252 TPVPGGVGPMTVAMLLKNTLLAAK 275
Cdd:cd05212  117 VPMTGGVGKLTVAMRMQNMVRSVR 140
NAD_bind_m-THF_DH cd01079
NAD binding domain of methylene-tetrahydrofolate dehydrogenase; The NAD-binding domain of ...
 173-271 7.86e-03

NAD binding domain of methylene-tetrahydrofolate dehydrogenase; The NAD-binding domain of methylene-tetrahydrofolate dehydrogenase (m-THF DH). M-THF is a versatile carrier of activated one-carbon units. The major one-carbon folate donors are N-5 methyltetrahydrofolate, N5,N10-m-THF, and N10-formayltetrahydrofolate. The oxidation of metabolic intermediate m-THF to m-THF requires the enzyme m-THF DH. M-THF DH is a component of an unusual monofunctional enzyme; in eukaryotes, m-THF DH is typically found as part of a multifunctional protein. NADP-dependent m-THF DHs in mammals, birds and yeast are components of a trifunctional enzyme with DH, cyclohydrolase, and synthetase activities. Certain eukaryotic cells also contain homodimeric bifunctional DH/cyclodrolase form. In bacteria, monofunctional DH, as well as bifunctional DH/cyclodrolase are found. In addition, yeast (S. cerevisiae) also express an monofunctional DH. This family contains only the monofunctional DHs from S. cerevisiae and certain bacteria. M-THF DH, like other amino acid DH-like NAD(P)-binding domains, is a member of the Rossmann fold superfamily which includes glutamate, leucine, and phenylalanine DHs, m-THF DH, methylene-tetrahydromethanopterin DH, m-THF DH/cyclohydrolase, Shikimate DH-like proteins, malate oxidoreductases, and glutamyl tRNA reductase. Amino acid DHs catalyze the deamination of amino acids to keto acids with NAD(P)+ as a cofactor. The NAD(P)-binding Rossmann fold superfamily includes a wide variety of protein families including NAD(P)- binding domains of alcohol DHs, tyrosine-dependent oxidoreductases, glyceraldehyde-3-phosphate DH, lactate/malate DHs, formate/glycerate DHs, siroheme synthases, 6-phosphogluconate DH, amino acid DHs, repressor rex, NAD-binding potassium channel domain, CoA-binding, and ornithine cyclodeaminase-like domains. These domains have an alpha-beta-alpha configuration. NAD binding involves numerous hydrogen and van der Waals contacts.


Pssm-ID: 133447 [Multi-domain]  Cd Length: 197  Bit Score: 36.64  E-value: 7.86e-03
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 568936126 173 TVTIAHRYTP----REQLKAHTQLADIIIVAAGIPRL---ITADMVREGAAVIDVGinyiqdpvtgktklvGDVDFEA-V 244
Cdd:cd01079  101 TRGESIRHEKhhvtDEEAMTLDCLSQSDVVITGVPSPnykVPTELLKDGAICINFA---------------SIKNFEPsV 165

                         90       100
                 ....*....|....*....|....*..
gi 568936126 245 KKKASFITPVpggVGPMTVAMLLKNTL 271
Cdd:cd01079  166 KEKASIYVPS---IGKVTIAMLLRNLL 189
AlaDh_PNT_C smart01002
Alanine dehydrogenase/PNT, C-terminal domain; Alanine dehydrogenase catalyzes the ...
 183-222 8.45e-03

Alanine dehydrogenase/PNT, C-terminal domain; Alanine dehydrogenase catalyzes the NAD-dependent reversible reductive amination of pyruvate into alanine.


Pssm-ID: 214966 [Multi-domain]  Cd Length: 149  Bit Score: 35.95  E-value: 8.45e-03
                           10        20        30        40
                   ....*....|....*....|....*....|....*....|....*...
gi 568936126   183 REQLKAHTQLADIIIVAAGI-----PRLITADMV---REGAAVIDVGI 222
Cdd:smart01002  74 AELLEEAVKEADLVIGAVLIpgakaPKLVTREMVksmKPGSVIVDVAA 121
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
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