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Conserved domains on  [gi|42734381|ref|NP_612476|]
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tRNA wybutosine-synthesizing protein 3 homolog isoform 1 [Homo sapiens]

Protein Classification

tRNA wybutosine-synthesizing 3 family protein( domain architecture ID 10496451)

tRNA wybutosine-synthesizing 3 (TYW3) family protein is a S-adenosyl-L-methionine (SAM)-dependent methyltransferase that acts as a component of the wybutosine biosynthesis pathway; wybutosine is a hyper modified guanosine with a tricyclic base found at the 3'-position adjacent to the anticodon of eukaryotic phenylalanine tRNA

Graphical summary

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List of domain hits

 Name Accession Description Interval E-value
TYW3 pfam02676
Methyltransferase TYW3; The methyltransferase TYW3 (tRNA-yW- synthesising protein 3) has been ...
 10-193 7.66e-81

Methyltransferase TYW3; The methyltransferase TYW3 (tRNA-yW- synthesising protein 3) has been identified in yeast to be involved in wybutosine (yW) biosynthesis. yW is a complexly modified guanosine residue that contains a tricyclic base and is found at the 3' position adjacent the anticodon of phenylalanine tRNA. TYW3 is an N-4 methylase that methylates yW-86 to yield yW-72 in an Ado-Met-dependent manner.


:

Pssm-ID: 460649  Cd Length: 206  Bit Score: 241.70  E-value: 7.66e-81
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 42734381    10 WKAQCLSK-----ADLSRKGSVDEDVVELVQFLNMRDQFFTTSSCAGRILLLDRG--INGFEVQKQNCCWLLVTHKLCVK 82
Cdd:pfam02676   1 RKAKILEElsseyTDASPKGSVDEDIRPLLDLINSHPDYVTTSSCSGRISVFLEGetDAKAGGKGGGGKWLFVSHDPVTV 80

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 42734381    83 DDVIVALKKA-------------NGDATLKFEPFVLHVQCRQLQDAQILHSMAIDSGFRNSGITVGKRGKTMLAVRSTHG 149
Cdd:pfam02676  81 EELLEALFGLkpgdgsadsddssSRLIWFKFEPMILHVLCRSLEHAQKLLSAARSAGFRESGITSLSKGRPIVAIRSGLR 160

                         170       180       190       200
                  ....*....|....*....|....*....|....*....|....*
gi 42734381   150 LEVPLSH-KGKLMVTEEYIDFLLNVANQKMEENKKRIERFYNCLQ 193
Cdd:pfam02676 161 LDVPIGYlRGKLLVSEEYLRFLVDIANERFEENKKRLERLYEALE 205
 
Name Accession Description Interval E-value
TYW3 pfam02676
Methyltransferase TYW3; The methyltransferase TYW3 (tRNA-yW- synthesising protein 3) has been ...
 10-193 7.66e-81

Methyltransferase TYW3; The methyltransferase TYW3 (tRNA-yW- synthesising protein 3) has been identified in yeast to be involved in wybutosine (yW) biosynthesis. yW is a complexly modified guanosine residue that contains a tricyclic base and is found at the 3' position adjacent the anticodon of phenylalanine tRNA. TYW3 is an N-4 methylase that methylates yW-86 to yield yW-72 in an Ado-Met-dependent manner.


Pssm-ID: 460649  Cd Length: 206  Bit Score: 241.70  E-value: 7.66e-81
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 42734381    10 WKAQCLSK-----ADLSRKGSVDEDVVELVQFLNMRDQFFTTSSCAGRILLLDRG--INGFEVQKQNCCWLLVTHKLCVK 82
Cdd:pfam02676   1 RKAKILEElsseyTDASPKGSVDEDIRPLLDLINSHPDYVTTSSCSGRISVFLEGetDAKAGGKGGGGKWLFVSHDPVTV 80

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 42734381    83 DDVIVALKKA-------------NGDATLKFEPFVLHVQCRQLQDAQILHSMAIDSGFRNSGITVGKRGKTMLAVRSTHG 149
Cdd:pfam02676  81 EELLEALFGLkpgdgsadsddssSRLIWFKFEPMILHVLCRSLEHAQKLLSAARSAGFRESGITSLSKGRPIVAIRSGLR 160

                         170       180       190       200
                  ....*....|....*....|....*....|....*....|....*
gi 42734381   150 LEVPLSH-KGKLMVTEEYIDFLLNVANQKMEENKKRIERFYNCLQ 193
Cdd:pfam02676 161 LDVPIGYlRGKLLVSEEYLRFLVDIANERFEENKKRLERLYEALE 205
PRK04235 PRK04235
hypothetical protein; Provisional
 22-198 2.22e-38

hypothetical protein; Provisional


Pssm-ID: 235262  Cd Length: 196  Bit Score: 132.72  E-value: 2.22e-38
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 42734381   22 RKGSVDEDVVELVQFLNMRDQFFTTSSCAGRILLLDRGINGFEVqKQNCCWLLVTHKLCVKDDVIVALKKAN-GDATLKF 100
Cdd:PRK04235  20 EIGYVDEDIIPLLDLINSLKNYYTTSSCSGRIGIIEAEMPSKGD-KKNSRWLGKWHHPVTPEEVLEALKKKQvGRLWLIV 98

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 42734381  101 EPFVLHVQCRQLQDAQILHSMAIDSGFRNSGITVGKRGKTMLAVRSTHGLEVPLSHKGKLMVTEEYIDFLLNVANQKMEE 180
Cdd:PRK04235  99 QGPILHVAARDLETAKKLLELAREAGFKHSGIKSISDKRVLVEIRTTERMDAPLGEDGKLVVDEEYLKFLVEVANEKLLR 178

                        170
                 ....*....|....*...
gi 42734381  181 NKKRIERFYNCLQHALER 198
Cdd:PRK04235 179 GKERLNRLEEELEELKEN 196
Tyw3 COG1590
tRNA(Phe) wybutosine-synthesizing methylase Tyw3 [Translation, ribosomal structure and ...
 6-192 8.00e-35

tRNA(Phe) wybutosine-synthesizing methylase Tyw3 [Translation, ribosomal structure and biogenesis]; tRNA(Phe) wybutosine-synthesizing methylase Tyw3 is part of the Pathway/BioSystem: tRNA modification


Pssm-ID: 441198  Cd Length: 194  Bit Score: 123.48  E-value: 8.00e-35
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 42734381   6 EFRKWKAQCLSKADL-SRKGSVDEDVVELVQFLNMRDQFFTTSSCAGRILLLDRGINGFevqKQNCCWLLVTHKLCVKDD 84
Cdd:COG1590   4 MWEERKKEALERLEEdLEIGYVDPDIIDLLDKINSLPDYYTTSSCSGRITVVDAPYPWD---KENSKIVFKWHRPVTVDE 80

                        90       100       110       120       130       140       150       160
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 42734381  85 VIVALKKANGDAT-LKFEPFVLHVQCRQLQDAQILHSMAIDSGFRNSGITVGKRGKTMLAVRSTHGLEVPLSHKGKLMVT 163
Cdd:COG1590  81 LLEALKKPSERRLwLIVQGPILHVYTRDLEEAKKLLKIAREAGFKHSGIKSISDKGVIVEIRSGERMDVPLRENGKLIVD 160

                       170       180
                ....*....|....*....|....*....
gi 42734381 164 EEYIDFLLNVANQKMEENKKRIERFYNCL 192
Cdd:COG1590 161 EEYLKRIVDVANEKLLKGKRRLKRLYEAL 189
 
Name Accession Description Interval E-value
TYW3 pfam02676
Methyltransferase TYW3; The methyltransferase TYW3 (tRNA-yW- synthesising protein 3) has been ...
 10-193 7.66e-81

Methyltransferase TYW3; The methyltransferase TYW3 (tRNA-yW- synthesising protein 3) has been identified in yeast to be involved in wybutosine (yW) biosynthesis. yW is a complexly modified guanosine residue that contains a tricyclic base and is found at the 3' position adjacent the anticodon of phenylalanine tRNA. TYW3 is an N-4 methylase that methylates yW-86 to yield yW-72 in an Ado-Met-dependent manner.


Pssm-ID: 460649  Cd Length: 206  Bit Score: 241.70  E-value: 7.66e-81
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 42734381    10 WKAQCLSK-----ADLSRKGSVDEDVVELVQFLNMRDQFFTTSSCAGRILLLDRG--INGFEVQKQNCCWLLVTHKLCVK 82
Cdd:pfam02676   1 RKAKILEElsseyTDASPKGSVDEDIRPLLDLINSHPDYVTTSSCSGRISVFLEGetDAKAGGKGGGGKWLFVSHDPVTV 80

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 42734381    83 DDVIVALKKA-------------NGDATLKFEPFVLHVQCRQLQDAQILHSMAIDSGFRNSGITVGKRGKTMLAVRSTHG 149
Cdd:pfam02676  81 EELLEALFGLkpgdgsadsddssSRLIWFKFEPMILHVLCRSLEHAQKLLSAARSAGFRESGITSLSKGRPIVAIRSGLR 160

                         170       180       190       200
                  ....*....|....*....|....*....|....*....|....*
gi 42734381   150 LEVPLSH-KGKLMVTEEYIDFLLNVANQKMEENKKRIERFYNCLQ 193
Cdd:pfam02676 161 LDVPIGYlRGKLLVSEEYLRFLVDIANERFEENKKRLERLYEALE 205
PRK04235 PRK04235
hypothetical protein; Provisional
 22-198 2.22e-38

hypothetical protein; Provisional


Pssm-ID: 235262  Cd Length: 196  Bit Score: 132.72  E-value: 2.22e-38
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 42734381   22 RKGSVDEDVVELVQFLNMRDQFFTTSSCAGRILLLDRGINGFEVqKQNCCWLLVTHKLCVKDDVIVALKKAN-GDATLKF 100
Cdd:PRK04235  20 EIGYVDEDIIPLLDLINSLKNYYTTSSCSGRIGIIEAEMPSKGD-KKNSRWLGKWHHPVTPEEVLEALKKKQvGRLWLIV 98

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 42734381  101 EPFVLHVQCRQLQDAQILHSMAIDSGFRNSGITVGKRGKTMLAVRSTHGLEVPLSHKGKLMVTEEYIDFLLNVANQKMEE 180
Cdd:PRK04235  99 QGPILHVAARDLETAKKLLELAREAGFKHSGIKSISDKRVLVEIRTTERMDAPLGEDGKLVVDEEYLKFLVEVANEKLLR 178

                        170
                 ....*....|....*...
gi 42734381  181 NKKRIERFYNCLQHALER 198
Cdd:PRK04235 179 GKERLNRLEEELEELKEN 196
Tyw3 COG1590
tRNA(Phe) wybutosine-synthesizing methylase Tyw3 [Translation, ribosomal structure and ...
 6-192 8.00e-35

tRNA(Phe) wybutosine-synthesizing methylase Tyw3 [Translation, ribosomal structure and biogenesis]; tRNA(Phe) wybutosine-synthesizing methylase Tyw3 is part of the Pathway/BioSystem: tRNA modification


Pssm-ID: 441198  Cd Length: 194  Bit Score: 123.48  E-value: 8.00e-35
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 42734381   6 EFRKWKAQCLSKADL-SRKGSVDEDVVELVQFLNMRDQFFTTSSCAGRILLLDRGINGFevqKQNCCWLLVTHKLCVKDD 84
Cdd:COG1590   4 MWEERKKEALERLEEdLEIGYVDPDIIDLLDKINSLPDYYTTSSCSGRITVVDAPYPWD---KENSKIVFKWHRPVTVDE 80

                        90       100       110       120       130       140       150       160
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 42734381  85 VIVALKKANGDAT-LKFEPFVLHVQCRQLQDAQILHSMAIDSGFRNSGITVGKRGKTMLAVRSTHGLEVPLSHKGKLMVT 163
Cdd:COG1590  81 LLEALKKPSERRLwLIVQGPILHVYTRDLEEAKKLLKIAREAGFKHSGIKSISDKGVIVEIRSGERMDVPLRENGKLIVD 160

                       170       180
                ....*....|....*....|....*....
gi 42734381 164 EEYIDFLLNVANQKMEENKKRIERFYNCL 192
Cdd:COG1590 161 EEYLKRIVDVANEKLLKGKRRLKRLYEAL 189
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
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