NCBI Home Page NCBI Site Search page NCBI Guide that lists and describes the NCBI resources
Conserved domains on  [gi|4502499|ref|NP_003947|]
View 

cholesterol 25-hydroxylase [Homo sapiens]

Protein Classification

sterol desaturase family protein( domain architecture ID 10513792)

sterol desaturase family protein is an oxidoreductase, similar to C-5 sterol desaturase, which catalyzes a dehydrogenation reaction to introduce a C5-6 double bond into substrates such as lathosterol and the B ring of ergosterol

Gene Ontology:  GO:0016491|GO:0008610
SCOP:  3002435

Graphical summary

 Zoom to residue level

show extra options »

Show site features     Horizontal zoom: ×

List of domain hits

 Name Accession Description Interval E-value
FA_hydroxylase pfam04116
Fatty acid hydroxylase; This entry includes fatty acid and carotene hydroxylases and sterol ...
 135-263 2.38e-22

Fatty acid hydroxylase; This entry includes fatty acid and carotene hydroxylases and sterol desaturases. Beta-carotene hydroxylase is involved in zeaxanthin synthesis by hydroxylating beta-carotene, but the enzyme may be involved in other pathways. This family includes C-5 sterol desaturase and C-4 sterol methyl oxidase. Members of this family are involved in cholesterol biosynthesis and biosynthesis a plant cuticular wax. These enzymes contain two copies of a HXHH motif which coordinate two irons at the catalytic centre. Members of this family are ER integral membrane proteins that share a novel mushroom-shaped fold consisting of four transmembrane (TM1-TM4) helices that anchor them to the membrane capped by a cytosolic domain containing the unique histidine- coordinating di metal centre.


:

Pssm-ID: 397991  Cd Length: 134  Bit Score: 89.41  E-value: 2.38e-22
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 4502499    135 FDMEFFVWHLLHHKVPWLYRTFHKVHHQNSSSFALATQYMSVWELFSLGFFDMMNVTLLGCHPLTTLTFHVVNIWLSVED 214
Cdd:pfam04116   8 FDFLFYWVHRLLHRLPWLWRRFHKVHHSSEAPNALTALRFHPLEALLFALLVLLPLLLLGLPLLAFLLGLLLGTLWYLFI 87

                          90       100       110       120       130
                  ....*....|....*....|....*....|....*....|....*....|
gi 4502499    215 HSGYNFPwstHRLVPFGWYGGVVHHDLHHSHFN-CNFAPYFTHWDKILGT 263
Cdd:pfam04116  88 HSGLLFP---LPWLLKRLLGTPRFHRLHHSKNEeYNFGVTFPLWDRLFGT 134
 
Name Accession Description Interval E-value
FA_hydroxylase pfam04116
Fatty acid hydroxylase; This entry includes fatty acid and carotene hydroxylases and sterol ...
 135-263 2.38e-22

Fatty acid hydroxylase; This entry includes fatty acid and carotene hydroxylases and sterol desaturases. Beta-carotene hydroxylase is involved in zeaxanthin synthesis by hydroxylating beta-carotene, but the enzyme may be involved in other pathways. This family includes C-5 sterol desaturase and C-4 sterol methyl oxidase. Members of this family are involved in cholesterol biosynthesis and biosynthesis a plant cuticular wax. These enzymes contain two copies of a HXHH motif which coordinate two irons at the catalytic centre. Members of this family are ER integral membrane proteins that share a novel mushroom-shaped fold consisting of four transmembrane (TM1-TM4) helices that anchor them to the membrane capped by a cytosolic domain containing the unique histidine- coordinating di metal centre.


Pssm-ID: 397991  Cd Length: 134  Bit Score: 89.41  E-value: 2.38e-22
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 4502499    135 FDMEFFVWHLLHHKVPWLYRTFHKVHHQNSSSFALATQYMSVWELFSLGFFDMMNVTLLGCHPLTTLTFHVVNIWLSVED 214
Cdd:pfam04116   8 FDFLFYWVHRLLHRLPWLWRRFHKVHHSSEAPNALTALRFHPLEALLFALLVLLPLLLLGLPLLAFLLGLLLGTLWYLFI 87

                          90       100       110       120       130
                  ....*....|....*....|....*....|....*....|....*....|
gi 4502499    215 HSGYNFPwstHRLVPFGWYGGVVHHDLHHSHFN-CNFAPYFTHWDKILGT 263
Cdd:pfam04116  88 HSGLLFP---LPWLLKRLLGTPRFHRLHHSKNEeYNFGVTFPLWDRLFGT 134
ERG3 COG3000
Sterol desaturase/sphingolipid hydroxylase, fatty acid hydroxylase superfamily [Lipid ...
 135-265 2.29e-14

Sterol desaturase/sphingolipid hydroxylase, fatty acid hydroxylase superfamily [Lipid transport and metabolism];


Pssm-ID: 442238  Cd Length: 158  Bit Score: 68.81  E-value: 2.29e-14
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 4502499  135 FDMEFFVWHLLHHKVPWLYRtFHKVHHQNSSSFALATQYMSVWELFSLGFFDMMNVTLLGCHPLTTLTFHVVNIWLSVED 214
Cdd:COG3000   9 LDFVYYWLHRLSHRVPLLWR-FHAVHHSSERMNLLTALRFHPLEILLSALLFLLPLALLGFPPEAVLLYLALNLLYQFFN 87

                        90       100       110       120       130
                ....*....|....*....|....*....|....*....|....*....|....*....
gi 4502499  215 HSGYNFPWSthrlvpfGWYGGVV----HHDLHHS----HFNCNFAPYFTHWDKILGTLR 265
Cdd:COG3000  88 HSNIRLPLD-------GPLEYVFvtpsHHRVHHSrnpeYLDKNYGGILSIWDRLFGTYR 139
 
Name Accession Description Interval E-value
FA_hydroxylase pfam04116
Fatty acid hydroxylase; This entry includes fatty acid and carotene hydroxylases and sterol ...
 135-263 2.38e-22

Fatty acid hydroxylase; This entry includes fatty acid and carotene hydroxylases and sterol desaturases. Beta-carotene hydroxylase is involved in zeaxanthin synthesis by hydroxylating beta-carotene, but the enzyme may be involved in other pathways. This family includes C-5 sterol desaturase and C-4 sterol methyl oxidase. Members of this family are involved in cholesterol biosynthesis and biosynthesis a plant cuticular wax. These enzymes contain two copies of a HXHH motif which coordinate two irons at the catalytic centre. Members of this family are ER integral membrane proteins that share a novel mushroom-shaped fold consisting of four transmembrane (TM1-TM4) helices that anchor them to the membrane capped by a cytosolic domain containing the unique histidine- coordinating di metal centre.


Pssm-ID: 397991  Cd Length: 134  Bit Score: 89.41  E-value: 2.38e-22
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 4502499    135 FDMEFFVWHLLHHKVPWLYRTFHKVHHQNSSSFALATQYMSVWELFSLGFFDMMNVTLLGCHPLTTLTFHVVNIWLSVED 214
Cdd:pfam04116   8 FDFLFYWVHRLLHRLPWLWRRFHKVHHSSEAPNALTALRFHPLEALLFALLVLLPLLLLGLPLLAFLLGLLLGTLWYLFI 87

                          90       100       110       120       130
                  ....*....|....*....|....*....|....*....|....*....|
gi 4502499    215 HSGYNFPwstHRLVPFGWYGGVVHHDLHHSHFN-CNFAPYFTHWDKILGT 263
Cdd:pfam04116  88 HSGLLFP---LPWLLKRLLGTPRFHRLHHSKNEeYNFGVTFPLWDRLFGT 134
ERG3 COG3000
Sterol desaturase/sphingolipid hydroxylase, fatty acid hydroxylase superfamily [Lipid ...
 135-265 2.29e-14

Sterol desaturase/sphingolipid hydroxylase, fatty acid hydroxylase superfamily [Lipid transport and metabolism];


Pssm-ID: 442238  Cd Length: 158  Bit Score: 68.81  E-value: 2.29e-14
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 4502499  135 FDMEFFVWHLLHHKVPWLYRtFHKVHHQNSSSFALATQYMSVWELFSLGFFDMMNVTLLGCHPLTTLTFHVVNIWLSVED 214
Cdd:COG3000   9 LDFVYYWLHRLSHRVPLLWR-FHAVHHSSERMNLLTALRFHPLEILLSALLFLLPLALLGFPPEAVLLYLALNLLYQFFN 87

                        90       100       110       120       130
                ....*....|....*....|....*....|....*....|....*....|....*....
gi 4502499  215 HSGYNFPWSthrlvpfGWYGGVV----HHDLHHS----HFNCNFAPYFTHWDKILGTLR 265
Cdd:COG3000  88 HSNIRLPLD-------GPLEYVFvtpsHHRVHHSrnpeYLDKNYGGILSIWDRLFGTYR 139
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
Help | Disclaimer | Write to the Help Desk
NCBI | NLM | NIH