NCBI Home Page NCBI Site Search page NCBI Guide that lists and describes the NCBI resources
Conserved domains on  [gi|51702526|ref|NP_003932|]
View 

actin nucleation-promoting factor WASL [Homo sapiens]

Protein Classification

Graphical summary

 Zoom to residue level

show extra options »

Show site features     Horizontal zoom: ×

List of domain hits

Name Accession Description Interval E-value
EVH1_WASP-like cd01205
WASP family proteins EVH1 domain; The Wiskott-Aldrich Syndrome Protein (WASP; also called ...
40-138 3.10e-51

WASP family proteins EVH1 domain; The Wiskott-Aldrich Syndrome Protein (WASP; also called Bee1p) and its homolog N (neuronal)-WASP are signal transduction proteins that promote actin polymerization in response to upstream intracellular signals. WAS is an X-linked recessive disease, characterized by eczema, immunodeficiency, and thrombocytopenia. The majority of patients with WAS, or a milder version of the disorder, X-linked thrombocytopenia (XLT), have point mutations in the EVH1 domain of WASP. WASP is an actin regulatory protein consisting of an N-terminal EVH1 domain called WH1 which binds LPPPEP peptides, a basic region (B), a GTP binding domain (GBP), a proline rich region, a WH2 domain, and a verprolin-cofilin-acidic motif (VCA) which activates the actin-related protein (Arp)2/3 actin nucleating complex. The B, GBD, and the proline-rich region are involved in autoinhibitory interactions that repress or block the activity of the VCA. Yeast members lack the GTP binding domain. The EVH1 domains are part of the PH domain superamily. There are 5 EVH1 subfamilies: Enables/VASP, Homer/Vesl, WASP, Dcp1, and Spred. Ligands are known for three of the EVH1 subfamilies, all of which bind proline-rich sequences: the Enabled/VASP family binds to FPPPP peptides, the Homer/Vesl family binds PPxxF peptides, and the WASP family binds LPPPEP peptides. EVH1 has a PH-like fold, despite having minimal sequence similarity to PH or PTB domains.


:

Pssm-ID: 269916  Cd Length: 101  Bit Score: 169.63  E-value: 3.10e-51
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 51702526  40 TMSSAVVQLYAADRN-CMWSKK-CSGVACLVKDNPQRSYFLRIFDIKDGKLLWEQELYNNFVYNSPRGYFHTFAGDTCQV 117
Cdd:cd01205   1 ILAAAVARLYYAEPDpDSWSYTgLTGALCFVKDNAKRSYFFRLVDLKTRGVVWEQELYEGFEYNQDRPFFHTFEGDECMI 80
                        90       100
                ....*....|....*....|.
gi 51702526 118 ALNFANEEEAKKFRKAVTDLL 138
Cdd:cd01205  81 GLNFADEDEAAAFYKKVQEKL 101
PBD pfam00786
P21-Rho-binding domain; Small domains that bind Cdc42p- and/or Rho-like small GTPases. Also ...
202-259 2.78e-18

P21-Rho-binding domain; Small domains that bind Cdc42p- and/or Rho-like small GTPases. Also known as the Cdc42/Rac interactive binding (CRIB).


:

Pssm-ID: 395634  Cd Length: 59  Bit Score: 78.51  E-value: 2.78e-18
                          10        20        30        40        50
                  ....*....|....*....|....*....|....*....|....*....|....*...
gi 51702526   202 DIGTPSNFQHIGHVGWDPNTGFDlNNLDPELKNLFDMCGISEAQLKDRETSKVIYDFI 259
Cdd:pfam00786   1 MISAPTNFKHTVHVGFDPDTGFF-TGLPPEWAKLLDSSGITEDEQKENPKAVLDVLKF 57
WH2_N-WASP_r1 cd22074
first tandem Wiskott Aldrich syndrome homology region 2 (WH2 motif) repeat found in human ...
402-428 7.55e-11

first tandem Wiskott Aldrich syndrome homology region 2 (WH2 motif) repeat found in human Neural Wiskott-Aldrich syndrome protein (N-WASP) and related domains; This subfamily includes the first tandem Wiskott-Aldrich syndrome protein (WASP)-homology domain 2 (WH2) found in human Neural Wiskott-Aldrich syndrome protein (N-WASP or Neural WASP) and related domains. N-WASP integrates various extracellular signals to control actin dynamics and cytoskeletal reorganization through activation of the actin related protein (Arp)2/3 complex. It interacts with actin via the WH2 domain. N-WASP plays an important role in the deactivation or attenuation of B cell receptor signaling. N-WASP regulates filopodia formation and membrane invagination, as compared to WAVE proteins that serve as Rac1 effectors in the formation of lamellipodia. Filopodia are thin, actin-rich surface projections that are extended and maintained by N-WASP together with CDC42. N-WASP also plays a role in the nucleus by regulating gene transcription, probably by promoting nuclear actin polymerization. It binds to HSF1/HSTF1 and forms a complex on heat shock promoter elements (HSE) that negatively regulates HSP90 expression. It also plays a role in dendrite spine morphogenesis. Unphosphorylated N-WASP is preferentially localized in the nucleus and in the cytoplasm when phosphorylated; it is exported from the nucleus by a nuclear export signal (NES)-dependent mechanism to the cytoplasm.


:

Pssm-ID: 409217  Cd Length: 27  Bit Score: 56.87  E-value: 7.55e-11
                        10        20
                ....*....|....*....|....*..
gi 51702526 402 TAGNKAALLDQIREGAQLKKVEQNSRP 428
Cdd:cd22074   1 AAGNKSALLDQIREGAQLKKVEQNSRP 27
WH2_hN-WASP_r2_like cd22075
second tandem Wiskott Aldrich syndrome homology region 2 (WH2 motif) repeat found in human ...
429-453 6.62e-10

second tandem Wiskott Aldrich syndrome homology region 2 (WH2 motif) repeat found in human Neural Wiskott-Aldrich syndrome protein (N-WASP) and related domains; This subfamily includes the second tandem Wiskott-Aldrich syndrome protein (WASP)-homology domain 2 (WH2) found in human Neural Wiskott-Aldrich syndrome protein (N-WASP or Neural WASP). N-WASP integrates various extracellular signals to control actin dynamics and cytoskeletal reorganization through activation of the actin related protein (Arp)2/3 complex. It interacts with actin via the WH2 domain. N-WASP plays an important role in the deactivation or attenuation of B cell receptor signaling. N-WASP regulates filopodia formation and membrane invagination, as compared to WAVE proteins that serve as Rac1 effectors in the formation of lamellipodia. Filopodia are thin, actin-rich surface projections that are extended and maintained by N-WASP together with CDC42. N-WASP also plays a role in the nucleus by regulating gene transcription, probably by promoting nuclear actin polymerization. It binds to HSF1/HSTF1 and forms a complex on heat shock promoter elements (HSE) that negatively regulates HSP90 expression. It also plays a role in dendrite spine morphogenesis. Unphosphorylated N-WASP is preferentially localized in the nucleus and in the cytoplasm when phosphorylated; it is exported from the nucleus by a nuclear export signal (NES)-dependent mechanism to the cytoplasm. This subfamily includes both tandem WH2 domains of mouse N-WASP.


:

Pssm-ID: 409218  Cd Length: 25  Bit Score: 54.00  E-value: 6.62e-10
                        10        20
                ....*....|....*....|....*
gi 51702526 429 VSCSGRDALLDQIRQGIQLKSVADG 453
Cdd:cd22075   1 VSSSGRDALLDQIRQGIQLKSVPDG 25
 
Name Accession Description Interval E-value
EVH1_WASP-like cd01205
WASP family proteins EVH1 domain; The Wiskott-Aldrich Syndrome Protein (WASP; also called ...
40-138 3.10e-51

WASP family proteins EVH1 domain; The Wiskott-Aldrich Syndrome Protein (WASP; also called Bee1p) and its homolog N (neuronal)-WASP are signal transduction proteins that promote actin polymerization in response to upstream intracellular signals. WAS is an X-linked recessive disease, characterized by eczema, immunodeficiency, and thrombocytopenia. The majority of patients with WAS, or a milder version of the disorder, X-linked thrombocytopenia (XLT), have point mutations in the EVH1 domain of WASP. WASP is an actin regulatory protein consisting of an N-terminal EVH1 domain called WH1 which binds LPPPEP peptides, a basic region (B), a GTP binding domain (GBP), a proline rich region, a WH2 domain, and a verprolin-cofilin-acidic motif (VCA) which activates the actin-related protein (Arp)2/3 actin nucleating complex. The B, GBD, and the proline-rich region are involved in autoinhibitory interactions that repress or block the activity of the VCA. Yeast members lack the GTP binding domain. The EVH1 domains are part of the PH domain superamily. There are 5 EVH1 subfamilies: Enables/VASP, Homer/Vesl, WASP, Dcp1, and Spred. Ligands are known for three of the EVH1 subfamilies, all of which bind proline-rich sequences: the Enabled/VASP family binds to FPPPP peptides, the Homer/Vesl family binds PPxxF peptides, and the WASP family binds LPPPEP peptides. EVH1 has a PH-like fold, despite having minimal sequence similarity to PH or PTB domains.


Pssm-ID: 269916  Cd Length: 101  Bit Score: 169.63  E-value: 3.10e-51
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 51702526  40 TMSSAVVQLYAADRN-CMWSKK-CSGVACLVKDNPQRSYFLRIFDIKDGKLLWEQELYNNFVYNSPRGYFHTFAGDTCQV 117
Cdd:cd01205   1 ILAAAVARLYYAEPDpDSWSYTgLTGALCFVKDNAKRSYFFRLVDLKTRGVVWEQELYEGFEYNQDRPFFHTFEGDECMI 80
                        90       100
                ....*....|....*....|.
gi 51702526 118 ALNFANEEEAKKFRKAVTDLL 138
Cdd:cd01205  81 GLNFADEDEAAAFYKKVQEKL 101
WH1 pfam00568
WH1 domain; WASp Homology domain 1 (WH1) domain. WASP is the protein that is defective in ...
31-138 1.02e-50

WH1 domain; WASp Homology domain 1 (WH1) domain. WASP is the protein that is defective in Wiskott-Aldrich syndrome (WAS). The majority of point mutations occur within the amino- terminal WH1 domain. The metabotropic glutamate receptors mGluR1alpha and mGluR5 bind a protein called homer, which is a WH1 domain homolog. A subset of WH1 domains has been termed a "EVH1" domain and appear to bind a polyproline motif.


Pssm-ID: 395450  Cd Length: 111  Bit Score: 168.78  E-value: 1.02e-50
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 51702526    31 FTFLGKKCVTMSSAVVQLYAAD--RNCMWSK-KCSGVACLVKDNPQRSYFLRIFDIKDGKLLWEQELYNNFVYNSPRGYF 107
Cdd:pfam00568   1 FSALGKKCQTICTAVAQVYLADpdNKRHWIKaKHSGVVCFVKDSPQNSYFIRLVDIQDGKVIWNQEIYPNMEYNQARPFF 80
                          90       100       110
                  ....*....|....*....|....*....|.
gi 51702526   108 HTFAGDTCQVALNFANEEEAKKFRKAVTDLL 138
Cdd:pfam00568  81 HTFADSRCVYGLNFASEEEATKFAKAVQEAL 111
WH1 smart00461
WASP homology region 1; Region of the Wiskott-Aldrich syndrome protein (WASp) that contains ...
34-138 5.55e-41

WASP homology region 1; Region of the Wiskott-Aldrich syndrome protein (WASp) that contains point mutations in the majority of patients with WAS. Unknown function. Ena-like WH1 domains bind polyproline-containing peptides, and that Homer contains a WH1 domain.


Pssm-ID: 214674  Cd Length: 106  Bit Score: 142.49  E-value: 5.55e-41
                           10        20        30        40        50        60        70        80
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 51702526     34 LGKKCVTMSSAVVQLYAADRNCmWSKKCSG-VACLVKDNPQRSYFLRIFDIKDG-KLLWEQELYNNFVYNSPRGYFHTFA 111
Cdd:smart00461   1 LGSQCIILARAVVQLYDADTKK-WVPTGEGgAANLVIDKNQRSYFFRIVGIKGQdKVIWNQELYKNFKYNQATPTFHQWA 79
                           90       100
                   ....*....|....*....|....*..
gi 51702526    112 GDTCQVALNFANEEEAKKFRKAVTDLL 138
Cdd:smart00461  80 DDKCVYGLNFASEEEAKKFRKKVLKAL 106
PBD pfam00786
P21-Rho-binding domain; Small domains that bind Cdc42p- and/or Rho-like small GTPases. Also ...
202-259 2.78e-18

P21-Rho-binding domain; Small domains that bind Cdc42p- and/or Rho-like small GTPases. Also known as the Cdc42/Rac interactive binding (CRIB).


Pssm-ID: 395634  Cd Length: 59  Bit Score: 78.51  E-value: 2.78e-18
                          10        20        30        40        50
                  ....*....|....*....|....*....|....*....|....*....|....*...
gi 51702526   202 DIGTPSNFQHIGHVGWDPNTGFDlNNLDPELKNLFDMCGISEAQLKDRETSKVIYDFI 259
Cdd:pfam00786   1 MISAPTNFKHTVHVGFDPDTGFF-TGLPPEWAKLLDSSGITEDEQKENPKAVLDVLKF 57
CRIB cd00132
PAK (p21 activated kinase) Binding Domain (PBD), binds Cdc42p- and/or Rho-like small GTPases; ...
201-243 4.77e-14

PAK (p21 activated kinase) Binding Domain (PBD), binds Cdc42p- and/or Rho-like small GTPases; also known as the Cdc42/Rac interactive binding (CRIB) motif; has been shown to inhibit transcriptional activation and cell transformation mediated by the Ras-Rac pathway. CRIB-containing effector proteins are functionally diverse and include serine/threonine kinases, tyrosine kinases, actin-binding proteins, and adapter molecules.


Pssm-ID: 238077  Cd Length: 42  Bit Score: 65.92  E-value: 4.77e-14
                        10        20        30        40
                ....*....|....*....|....*....|....*....|...
gi 51702526 201 ADIGTPSNFQHIGHVGWDPNtGFDLNNLDPELKNLFDMCGISE 243
Cdd:cd00132   1 MEISTPTDFKHISHVGWDGV-GFDGANLPPDLQSLFQTAGISA 42
WH2_N-WASP_r1 cd22074
first tandem Wiskott Aldrich syndrome homology region 2 (WH2 motif) repeat found in human ...
402-428 7.55e-11

first tandem Wiskott Aldrich syndrome homology region 2 (WH2 motif) repeat found in human Neural Wiskott-Aldrich syndrome protein (N-WASP) and related domains; This subfamily includes the first tandem Wiskott-Aldrich syndrome protein (WASP)-homology domain 2 (WH2) found in human Neural Wiskott-Aldrich syndrome protein (N-WASP or Neural WASP) and related domains. N-WASP integrates various extracellular signals to control actin dynamics and cytoskeletal reorganization through activation of the actin related protein (Arp)2/3 complex. It interacts with actin via the WH2 domain. N-WASP plays an important role in the deactivation or attenuation of B cell receptor signaling. N-WASP regulates filopodia formation and membrane invagination, as compared to WAVE proteins that serve as Rac1 effectors in the formation of lamellipodia. Filopodia are thin, actin-rich surface projections that are extended and maintained by N-WASP together with CDC42. N-WASP also plays a role in the nucleus by regulating gene transcription, probably by promoting nuclear actin polymerization. It binds to HSF1/HSTF1 and forms a complex on heat shock promoter elements (HSE) that negatively regulates HSP90 expression. It also plays a role in dendrite spine morphogenesis. Unphosphorylated N-WASP is preferentially localized in the nucleus and in the cytoplasm when phosphorylated; it is exported from the nucleus by a nuclear export signal (NES)-dependent mechanism to the cytoplasm.


Pssm-ID: 409217  Cd Length: 27  Bit Score: 56.87  E-value: 7.55e-11
                        10        20
                ....*....|....*....|....*..
gi 51702526 402 TAGNKAALLDQIREGAQLKKVEQNSRP 428
Cdd:cd22074   1 AAGNKSALLDQIREGAQLKKVEQNSRP 27
WH2_hN-WASP_r2_like cd22075
second tandem Wiskott Aldrich syndrome homology region 2 (WH2 motif) repeat found in human ...
429-453 6.62e-10

second tandem Wiskott Aldrich syndrome homology region 2 (WH2 motif) repeat found in human Neural Wiskott-Aldrich syndrome protein (N-WASP) and related domains; This subfamily includes the second tandem Wiskott-Aldrich syndrome protein (WASP)-homology domain 2 (WH2) found in human Neural Wiskott-Aldrich syndrome protein (N-WASP or Neural WASP). N-WASP integrates various extracellular signals to control actin dynamics and cytoskeletal reorganization through activation of the actin related protein (Arp)2/3 complex. It interacts with actin via the WH2 domain. N-WASP plays an important role in the deactivation or attenuation of B cell receptor signaling. N-WASP regulates filopodia formation and membrane invagination, as compared to WAVE proteins that serve as Rac1 effectors in the formation of lamellipodia. Filopodia are thin, actin-rich surface projections that are extended and maintained by N-WASP together with CDC42. N-WASP also plays a role in the nucleus by regulating gene transcription, probably by promoting nuclear actin polymerization. It binds to HSF1/HSTF1 and forms a complex on heat shock promoter elements (HSE) that negatively regulates HSP90 expression. It also plays a role in dendrite spine morphogenesis. Unphosphorylated N-WASP is preferentially localized in the nucleus and in the cytoplasm when phosphorylated; it is exported from the nucleus by a nuclear export signal (NES)-dependent mechanism to the cytoplasm. This subfamily includes both tandem WH2 domains of mouse N-WASP.


Pssm-ID: 409218  Cd Length: 25  Bit Score: 54.00  E-value: 6.62e-10
                        10        20
                ....*....|....*....|....*
gi 51702526 429 VSCSGRDALLDQIRQGIQLKSVADG 453
Cdd:cd22075   1 VSSSGRDALLDQIRQGIQLKSVPDG 25
PBD smart00285
P21-Rho-binding domain; Small domains that bind Cdc42p- and/or Rho-like small GTPases. Also ...
203-239 9.68e-10

P21-Rho-binding domain; Small domains that bind Cdc42p- and/or Rho-like small GTPases. Also known as the Cdc42/Rac interactive binding (CRIB).


Pssm-ID: 197628  Cd Length: 36  Bit Score: 53.75  E-value: 9.68e-10
                           10        20        30
                   ....*....|....*....|....*....|....*..
gi 51702526    203 IGTPSNFQHIGHVGWDPNTGfDLNNLDPELKNLFDMC 239
Cdd:smart00285   1 ISTPTNFKHIAHVGFDGQTG-GFTGLPTEWKSLLKTS 36
WH2 smart00246
Wiskott Aldrich syndrome homology region 2; Wiskott Aldrich syndrome homology region 2 / ...
433-450 6.91e-05

Wiskott Aldrich syndrome homology region 2; Wiskott Aldrich syndrome homology region 2 / actin-binding motif


Pssm-ID: 128542  Cd Length: 18  Bit Score: 39.49  E-value: 6.91e-05
                           10
                   ....*....|....*...
gi 51702526    433 GRDALLDQIRQGIQLKSV 450
Cdd:smart00246   1 ARSALLAQIRQGKKLKKV 18
WH2 pfam02205
WH2 motif; The WH2 motif (for Wiskott Aldrich syndrome homology region 2) has been shown in ...
403-425 4.54e-04

WH2 motif; The WH2 motif (for Wiskott Aldrich syndrome homology region 2) has been shown in WASP and Scar1 (mammalian homolog) to be the region that interacts with actin.


Pssm-ID: 460490  Cd Length: 28  Bit Score: 37.48  E-value: 4.54e-04
                          10        20
                  ....*....|....*....|...
gi 51702526   403 AGNKAALLDQIREGAQLKKVEQN 425
Cdd:pfam02205   2 GGGRGALLADIRAGKKLKKVEET 24
WH2 pfam02205
WH2 motif; The WH2 motif (for Wiskott Aldrich syndrome homology region 2) has been shown in ...
431-451 5.15e-04

WH2 motif; The WH2 motif (for Wiskott Aldrich syndrome homology region 2) has been shown in WASP and Scar1 (mammalian homolog) to be the region that interacts with actin.


Pssm-ID: 460490  Cd Length: 28  Bit Score: 37.48  E-value: 5.15e-04
                          10        20
                  ....*....|....*....|.
gi 51702526   431 CSGRDALLDQIRQGIQLKSVA 451
Cdd:pfam02205   2 GGGRGALLADIRAGKKLKKVE 22
WH2 smart00246
Wiskott Aldrich syndrome homology region 2; Wiskott Aldrich syndrome homology region 2 / ...
407-422 2.74e-03

Wiskott Aldrich syndrome homology region 2; Wiskott Aldrich syndrome homology region 2 / actin-binding motif


Pssm-ID: 128542  Cd Length: 18  Bit Score: 35.25  E-value: 2.74e-03
                           10
                   ....*....|....*.
gi 51702526    407 AALLDQIREGAQLKKV 422
Cdd:smart00246   3 SALLAQIRQGKKLKKV 18
 
Name Accession Description Interval E-value
EVH1_WASP-like cd01205
WASP family proteins EVH1 domain; The Wiskott-Aldrich Syndrome Protein (WASP; also called ...
40-138 3.10e-51

WASP family proteins EVH1 domain; The Wiskott-Aldrich Syndrome Protein (WASP; also called Bee1p) and its homolog N (neuronal)-WASP are signal transduction proteins that promote actin polymerization in response to upstream intracellular signals. WAS is an X-linked recessive disease, characterized by eczema, immunodeficiency, and thrombocytopenia. The majority of patients with WAS, or a milder version of the disorder, X-linked thrombocytopenia (XLT), have point mutations in the EVH1 domain of WASP. WASP is an actin regulatory protein consisting of an N-terminal EVH1 domain called WH1 which binds LPPPEP peptides, a basic region (B), a GTP binding domain (GBP), a proline rich region, a WH2 domain, and a verprolin-cofilin-acidic motif (VCA) which activates the actin-related protein (Arp)2/3 actin nucleating complex. The B, GBD, and the proline-rich region are involved in autoinhibitory interactions that repress or block the activity of the VCA. Yeast members lack the GTP binding domain. The EVH1 domains are part of the PH domain superamily. There are 5 EVH1 subfamilies: Enables/VASP, Homer/Vesl, WASP, Dcp1, and Spred. Ligands are known for three of the EVH1 subfamilies, all of which bind proline-rich sequences: the Enabled/VASP family binds to FPPPP peptides, the Homer/Vesl family binds PPxxF peptides, and the WASP family binds LPPPEP peptides. EVH1 has a PH-like fold, despite having minimal sequence similarity to PH or PTB domains.


Pssm-ID: 269916  Cd Length: 101  Bit Score: 169.63  E-value: 3.10e-51
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 51702526  40 TMSSAVVQLYAADRN-CMWSKK-CSGVACLVKDNPQRSYFLRIFDIKDGKLLWEQELYNNFVYNSPRGYFHTFAGDTCQV 117
Cdd:cd01205   1 ILAAAVARLYYAEPDpDSWSYTgLTGALCFVKDNAKRSYFFRLVDLKTRGVVWEQELYEGFEYNQDRPFFHTFEGDECMI 80
                        90       100
                ....*....|....*....|.
gi 51702526 118 ALNFANEEEAKKFRKAVTDLL 138
Cdd:cd01205  81 GLNFADEDEAAAFYKKVQEKL 101
WH1 pfam00568
WH1 domain; WASp Homology domain 1 (WH1) domain. WASP is the protein that is defective in ...
31-138 1.02e-50

WH1 domain; WASp Homology domain 1 (WH1) domain. WASP is the protein that is defective in Wiskott-Aldrich syndrome (WAS). The majority of point mutations occur within the amino- terminal WH1 domain. The metabotropic glutamate receptors mGluR1alpha and mGluR5 bind a protein called homer, which is a WH1 domain homolog. A subset of WH1 domains has been termed a "EVH1" domain and appear to bind a polyproline motif.


Pssm-ID: 395450  Cd Length: 111  Bit Score: 168.78  E-value: 1.02e-50
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 51702526    31 FTFLGKKCVTMSSAVVQLYAAD--RNCMWSK-KCSGVACLVKDNPQRSYFLRIFDIKDGKLLWEQELYNNFVYNSPRGYF 107
Cdd:pfam00568   1 FSALGKKCQTICTAVAQVYLADpdNKRHWIKaKHSGVVCFVKDSPQNSYFIRLVDIQDGKVIWNQEIYPNMEYNQARPFF 80
                          90       100       110
                  ....*....|....*....|....*....|.
gi 51702526   108 HTFAGDTCQVALNFANEEEAKKFRKAVTDLL 138
Cdd:pfam00568  81 HTFADSRCVYGLNFASEEEATKFAKAVQEAL 111
EVH1_family cd00837
EVH1 (Drosophila Enabled (Ena)/Vasodilator-stimulated phosphoprotein (VASP) homology 1) domain; ...
40-138 2.56e-42

EVH1 (Drosophila Enabled (Ena)/Vasodilator-stimulated phosphoprotein (VASP) homology 1) domain; The EVH1 domains are part of the PH domain superfamily. EVH1 subfamilies include Enables/VASP, Homer/Vesl, WASP, and Spred. Ligands are known for three of the EVH1 subfamilies, all of which bind proline-rich sequences: the Enabled/VASP family binds to FPPPP peptides, the Homer/Vesl family binds PPxxF peptides, and the WASP family binds LPPPEP peptides. EVH1 has a PH-like fold, despite having minimal sequence similarity to PH or PTB domains.


Pssm-ID: 269909  Cd Length: 103  Bit Score: 146.07  E-value: 2.56e-42
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 51702526  40 TMSSAVVQLYAADR-NCMWSK---KCSGVACLVKDNPQRSYFLRIFDIKDGKLLWEQELYNNFVYNSPRGYFHTFAGDTC 115
Cdd:cd00837   1 SIFSARAHVMQIDDsNKNWVPaggKGASRVSYFKDTTRNSFRIIGVDIKDKKVVINCTITKNLVYNKATQTFHQWADDRT 80
                        90       100
                ....*....|....*....|...
gi 51702526 116 QVALNFANEEEAKKFRKAVTDLL 138
Cdd:cd00837  81 VFGLNFASEEDATKFAEAVQEAL 103
WH1 smart00461
WASP homology region 1; Region of the Wiskott-Aldrich syndrome protein (WASp) that contains ...
34-138 5.55e-41

WASP homology region 1; Region of the Wiskott-Aldrich syndrome protein (WASp) that contains point mutations in the majority of patients with WAS. Unknown function. Ena-like WH1 domains bind polyproline-containing peptides, and that Homer contains a WH1 domain.


Pssm-ID: 214674  Cd Length: 106  Bit Score: 142.49  E-value: 5.55e-41
                           10        20        30        40        50        60        70        80
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 51702526     34 LGKKCVTMSSAVVQLYAADRNCmWSKKCSG-VACLVKDNPQRSYFLRIFDIKDG-KLLWEQELYNNFVYNSPRGYFHTFA 111
Cdd:smart00461   1 LGSQCIILARAVVQLYDADTKK-WVPTGEGgAANLVIDKNQRSYFFRIVGIKGQdKVIWNQELYKNFKYNQATPTFHQWA 79
                           90       100
                   ....*....|....*....|....*..
gi 51702526    112 GDTCQVALNFANEEEAKKFRKAVTDLL 138
Cdd:smart00461  80 DDKCVYGLNFASEEEAKKFRKKVLKAL 106
PBD pfam00786
P21-Rho-binding domain; Small domains that bind Cdc42p- and/or Rho-like small GTPases. Also ...
202-259 2.78e-18

P21-Rho-binding domain; Small domains that bind Cdc42p- and/or Rho-like small GTPases. Also known as the Cdc42/Rac interactive binding (CRIB).


Pssm-ID: 395634  Cd Length: 59  Bit Score: 78.51  E-value: 2.78e-18
                          10        20        30        40        50
                  ....*....|....*....|....*....|....*....|....*....|....*...
gi 51702526   202 DIGTPSNFQHIGHVGWDPNTGFDlNNLDPELKNLFDMCGISEAQLKDRETSKVIYDFI 259
Cdd:pfam00786   1 MISAPTNFKHTVHVGFDPDTGFF-TGLPPEWAKLLDSSGITEDEQKENPKAVLDVLKF 57
CRIB cd00132
PAK (p21 activated kinase) Binding Domain (PBD), binds Cdc42p- and/or Rho-like small GTPases; ...
201-243 4.77e-14

PAK (p21 activated kinase) Binding Domain (PBD), binds Cdc42p- and/or Rho-like small GTPases; also known as the Cdc42/Rac interactive binding (CRIB) motif; has been shown to inhibit transcriptional activation and cell transformation mediated by the Ras-Rac pathway. CRIB-containing effector proteins are functionally diverse and include serine/threonine kinases, tyrosine kinases, actin-binding proteins, and adapter molecules.


Pssm-ID: 238077  Cd Length: 42  Bit Score: 65.92  E-value: 4.77e-14
                        10        20        30        40
                ....*....|....*....|....*....|....*....|...
gi 51702526 201 ADIGTPSNFQHIGHVGWDPNtGFDLNNLDPELKNLFDMCGISE 243
Cdd:cd00132   1 MEISTPTDFKHISHVGWDGV-GFDGANLPPDLQSLFQTAGISA 42
WH2_N-WASP_r1 cd22074
first tandem Wiskott Aldrich syndrome homology region 2 (WH2 motif) repeat found in human ...
402-428 7.55e-11

first tandem Wiskott Aldrich syndrome homology region 2 (WH2 motif) repeat found in human Neural Wiskott-Aldrich syndrome protein (N-WASP) and related domains; This subfamily includes the first tandem Wiskott-Aldrich syndrome protein (WASP)-homology domain 2 (WH2) found in human Neural Wiskott-Aldrich syndrome protein (N-WASP or Neural WASP) and related domains. N-WASP integrates various extracellular signals to control actin dynamics and cytoskeletal reorganization through activation of the actin related protein (Arp)2/3 complex. It interacts with actin via the WH2 domain. N-WASP plays an important role in the deactivation or attenuation of B cell receptor signaling. N-WASP regulates filopodia formation and membrane invagination, as compared to WAVE proteins that serve as Rac1 effectors in the formation of lamellipodia. Filopodia are thin, actin-rich surface projections that are extended and maintained by N-WASP together with CDC42. N-WASP also plays a role in the nucleus by regulating gene transcription, probably by promoting nuclear actin polymerization. It binds to HSF1/HSTF1 and forms a complex on heat shock promoter elements (HSE) that negatively regulates HSP90 expression. It also plays a role in dendrite spine morphogenesis. Unphosphorylated N-WASP is preferentially localized in the nucleus and in the cytoplasm when phosphorylated; it is exported from the nucleus by a nuclear export signal (NES)-dependent mechanism to the cytoplasm.


Pssm-ID: 409217  Cd Length: 27  Bit Score: 56.87  E-value: 7.55e-11
                        10        20
                ....*....|....*....|....*..
gi 51702526 402 TAGNKAALLDQIREGAQLKKVEQNSRP 428
Cdd:cd22074   1 AAGNKSALLDQIREGAQLKKVEQNSRP 27
WH2_hN-WASP_r2_like cd22075
second tandem Wiskott Aldrich syndrome homology region 2 (WH2 motif) repeat found in human ...
429-453 6.62e-10

second tandem Wiskott Aldrich syndrome homology region 2 (WH2 motif) repeat found in human Neural Wiskott-Aldrich syndrome protein (N-WASP) and related domains; This subfamily includes the second tandem Wiskott-Aldrich syndrome protein (WASP)-homology domain 2 (WH2) found in human Neural Wiskott-Aldrich syndrome protein (N-WASP or Neural WASP). N-WASP integrates various extracellular signals to control actin dynamics and cytoskeletal reorganization through activation of the actin related protein (Arp)2/3 complex. It interacts with actin via the WH2 domain. N-WASP plays an important role in the deactivation or attenuation of B cell receptor signaling. N-WASP regulates filopodia formation and membrane invagination, as compared to WAVE proteins that serve as Rac1 effectors in the formation of lamellipodia. Filopodia are thin, actin-rich surface projections that are extended and maintained by N-WASP together with CDC42. N-WASP also plays a role in the nucleus by regulating gene transcription, probably by promoting nuclear actin polymerization. It binds to HSF1/HSTF1 and forms a complex on heat shock promoter elements (HSE) that negatively regulates HSP90 expression. It also plays a role in dendrite spine morphogenesis. Unphosphorylated N-WASP is preferentially localized in the nucleus and in the cytoplasm when phosphorylated; it is exported from the nucleus by a nuclear export signal (NES)-dependent mechanism to the cytoplasm. This subfamily includes both tandem WH2 domains of mouse N-WASP.


Pssm-ID: 409218  Cd Length: 25  Bit Score: 54.00  E-value: 6.62e-10
                        10        20
                ....*....|....*....|....*
gi 51702526 429 VSCSGRDALLDQIRQGIQLKSVADG 453
Cdd:cd22075   1 VSSSGRDALLDQIRQGIQLKSVPDG 25
PBD smart00285
P21-Rho-binding domain; Small domains that bind Cdc42p- and/or Rho-like small GTPases. Also ...
203-239 9.68e-10

P21-Rho-binding domain; Small domains that bind Cdc42p- and/or Rho-like small GTPases. Also known as the Cdc42/Rac interactive binding (CRIB).


Pssm-ID: 197628  Cd Length: 36  Bit Score: 53.75  E-value: 9.68e-10
                           10        20        30
                   ....*....|....*....|....*....|....*..
gi 51702526    203 IGTPSNFQHIGHVGWDPNTGfDLNNLDPELKNLFDMC 239
Cdd:smart00285   1 ISTPTNFKHIAHVGFDGQTG-GFTGLPTEWKSLLKTS 36
WH2_N_WASP cd22058
first and second of two tandem Wiskott Aldrich syndrome homology region 2 (WH2 motif) repeats ...
430-452 2.65e-08

first and second of two tandem Wiskott Aldrich syndrome homology region 2 (WH2 motif) repeats found in Neural Wiskott-Aldrich syndrome protein (N-WASP); This family contains both tandem Wiskott-Aldrich syndrome protein (WASP)-homology domain 2 (WH2) repeats found in the Neural Wiskott-Aldrich syndrome protein (N-WASP or Neural WASP); N-WASP contains two tandem WH2 domains. N-WASP integrates various extracellular signals to control actin dynamics and cytoskeletal reorganization through activation of the actin related protein (Arp)2/3 complex. It interacts with actin via the WH2 domain. N-WASP plays an important role in the deactivation or attenuation of B cell receptor signaling. N-WASP regulates filopodia formation and membrane invagination, as compared to WAVE proteins that serve as Rac1 effectors in the formation of lamellipodia. Filopodia are thin, actin-rich surface projections that are extended and maintained by N-WASP together with CDC42. N-WASP also plays a role in the nucleus by regulating gene transcription, probably by promoting nuclear actin polymerization. It binds to HSF1/HSTF1 and forms a complex on heat shock promoter elements (HSE) that negatively regulates HSP90 expression. It also plays a role in dendrite spine morphogenesis. Unphosphorylated N-WASP is preferentially localized in the nucleus and in the cytoplasm when phosphorylated; it is exported from the nucleus by a nuclear export signal (NES)-dependent mechanism to the cytoplasm.


Pssm-ID: 409201  Cd Length: 23  Bit Score: 49.52  E-value: 2.65e-08
                        10        20
                ....*....|....*....|...
gi 51702526 430 SCSGRDALLDQIRQGIQLKSVAD 452
Cdd:cd22058   1 SCSGRDALLDQIRQGIQLKSVED 23
WH2_N_WASP cd22058
first and second of two tandem Wiskott Aldrich syndrome homology region 2 (WH2 motif) repeats ...
402-424 1.74e-06

first and second of two tandem Wiskott Aldrich syndrome homology region 2 (WH2 motif) repeats found in Neural Wiskott-Aldrich syndrome protein (N-WASP); This family contains both tandem Wiskott-Aldrich syndrome protein (WASP)-homology domain 2 (WH2) repeats found in the Neural Wiskott-Aldrich syndrome protein (N-WASP or Neural WASP); N-WASP contains two tandem WH2 domains. N-WASP integrates various extracellular signals to control actin dynamics and cytoskeletal reorganization through activation of the actin related protein (Arp)2/3 complex. It interacts with actin via the WH2 domain. N-WASP plays an important role in the deactivation or attenuation of B cell receptor signaling. N-WASP regulates filopodia formation and membrane invagination, as compared to WAVE proteins that serve as Rac1 effectors in the formation of lamellipodia. Filopodia are thin, actin-rich surface projections that are extended and maintained by N-WASP together with CDC42. N-WASP also plays a role in the nucleus by regulating gene transcription, probably by promoting nuclear actin polymerization. It binds to HSF1/HSTF1 and forms a complex on heat shock promoter elements (HSE) that negatively regulates HSP90 expression. It also plays a role in dendrite spine morphogenesis. Unphosphorylated N-WASP is preferentially localized in the nucleus and in the cytoplasm when phosphorylated; it is exported from the nucleus by a nuclear export signal (NES)-dependent mechanism to the cytoplasm.


Pssm-ID: 409201  Cd Length: 23  Bit Score: 44.51  E-value: 1.74e-06
                        10        20
                ....*....|....*....|...
gi 51702526 402 TAGNKAALLDQIREGAQLKKVEQ 424
Cdd:cd22058   1 SCSGRDALLDQIRQGIQLKSVED 23
CRIB_PAK_like cd01093
PAK (p21 activated kinase) Binding Domain (PBD), binds Cdc42p- and/or Rho-like small GTPases; ...
203-222 1.65e-05

PAK (p21 activated kinase) Binding Domain (PBD), binds Cdc42p- and/or Rho-like small GTPases; also known as the Cdc42/Rac interactive binding (CRIB) motif; has been shown to inhibit transcriptional activation and cell transformation mediated by the Ras-Rac pathway. This subgroup of CRIB/PBD-domains is found N-terminal of Serine/Threonine kinase domains in PAK and PAK-like proteins.


Pssm-ID: 238526  Cd Length: 46  Bit Score: 41.87  E-value: 1.65e-05
                        10        20
                ....*....|....*....|
gi 51702526 203 IGTPSNFQHIGHVGWDPNTG 222
Cdd:cd01093   3 ISSPTNFKHRVHVGFDPQTG 22
WH2 smart00246
Wiskott Aldrich syndrome homology region 2; Wiskott Aldrich syndrome homology region 2 / ...
433-450 6.91e-05

Wiskott Aldrich syndrome homology region 2; Wiskott Aldrich syndrome homology region 2 / actin-binding motif


Pssm-ID: 128542  Cd Length: 18  Bit Score: 39.49  E-value: 6.91e-05
                           10
                   ....*....|....*...
gi 51702526    433 GRDALLDQIRQGIQLKSV 450
Cdd:smart00246   1 ARSALLAQIRQGKKLKKV 18
WH2 pfam02205
WH2 motif; The WH2 motif (for Wiskott Aldrich syndrome homology region 2) has been shown in ...
403-425 4.54e-04

WH2 motif; The WH2 motif (for Wiskott Aldrich syndrome homology region 2) has been shown in WASP and Scar1 (mammalian homolog) to be the region that interacts with actin.


Pssm-ID: 460490  Cd Length: 28  Bit Score: 37.48  E-value: 4.54e-04
                          10        20
                  ....*....|....*....|...
gi 51702526   403 AGNKAALLDQIREGAQLKKVEQN 425
Cdd:pfam02205   2 GGGRGALLADIRAGKKLKKVEET 24
WH2 pfam02205
WH2 motif; The WH2 motif (for Wiskott Aldrich syndrome homology region 2) has been shown in ...
431-451 5.15e-04

WH2 motif; The WH2 motif (for Wiskott Aldrich syndrome homology region 2) has been shown in WASP and Scar1 (mammalian homolog) to be the region that interacts with actin.


Pssm-ID: 460490  Cd Length: 28  Bit Score: 37.48  E-value: 5.15e-04
                          10        20
                  ....*....|....*....|.
gi 51702526   431 CSGRDALLDQIRQGIQLKSVA 451
Cdd:pfam02205   2 GGGRGALLADIRAGKKLKKVE 22
WH2 smart00246
Wiskott Aldrich syndrome homology region 2; Wiskott Aldrich syndrome homology region 2 / ...
407-422 2.74e-03

Wiskott Aldrich syndrome homology region 2; Wiskott Aldrich syndrome homology region 2 / actin-binding motif


Pssm-ID: 128542  Cd Length: 18  Bit Score: 35.25  E-value: 2.74e-03
                           10
                   ....*....|....*.
gi 51702526    407 AALLDQIREGAQLKKV 422
Cdd:smart00246   3 SALLAQIRQGKKLKKV 18
WH2_hN-WASP_r2_like cd22075
second tandem Wiskott Aldrich syndrome homology region 2 (WH2 motif) repeat found in human ...
401-425 4.29e-03

second tandem Wiskott Aldrich syndrome homology region 2 (WH2 motif) repeat found in human Neural Wiskott-Aldrich syndrome protein (N-WASP) and related domains; This subfamily includes the second tandem Wiskott-Aldrich syndrome protein (WASP)-homology domain 2 (WH2) found in human Neural Wiskott-Aldrich syndrome protein (N-WASP or Neural WASP). N-WASP integrates various extracellular signals to control actin dynamics and cytoskeletal reorganization through activation of the actin related protein (Arp)2/3 complex. It interacts with actin via the WH2 domain. N-WASP plays an important role in the deactivation or attenuation of B cell receptor signaling. N-WASP regulates filopodia formation and membrane invagination, as compared to WAVE proteins that serve as Rac1 effectors in the formation of lamellipodia. Filopodia are thin, actin-rich surface projections that are extended and maintained by N-WASP together with CDC42. N-WASP also plays a role in the nucleus by regulating gene transcription, probably by promoting nuclear actin polymerization. It binds to HSF1/HSTF1 and forms a complex on heat shock promoter elements (HSE) that negatively regulates HSP90 expression. It also plays a role in dendrite spine morphogenesis. Unphosphorylated N-WASP is preferentially localized in the nucleus and in the cytoplasm when phosphorylated; it is exported from the nucleus by a nuclear export signal (NES)-dependent mechanism to the cytoplasm. This subfamily includes both tandem WH2 domains of mouse N-WASP.


Pssm-ID: 409218  Cd Length: 25  Bit Score: 34.74  E-value: 4.29e-03
                        10        20
                ....*....|....*....|....*
gi 51702526 401 TTAGNKAALLDQIREGAQLKKVEQN 425
Cdd:cd22075   1 VSSSGRDALLDQIRQGIQLKSVPDG 25
WH2 cd21762
Wiskott-Aldrich Syndrome Homology (WASP) region 2 (WH2 motif), and similar proteins; This ...
430-450 5.48e-03

Wiskott-Aldrich Syndrome Homology (WASP) region 2 (WH2 motif), and similar proteins; This family contains the Wiskott-Aldrich syndrome protein (WASP)-homology domain 2 (WH2) as well as thymosin-beta (Tbeta; also called beta-thymosin or betaT) domains that are small, widespread intrinsically disordered actin-binding peptides displaying significant sequence variability and different regulations of actin self-assembly in motile and morphogenetic processes. These WH2/betaT peptides are identified by a central consensus actin-binding motif LKKT/V flanked by variable N-terminal and C-terminal extensions; the betaT shares a more extended and conserved C-terminal half than WH2. These single or repeated domains are found in actin-binding proteins (ABPs) such as the hematopoietic-specific protein WASP, its ubiquitously expressed ortholog neural-WASP (N-WASP), WASP-interacting protein (WAS/WASL-interacting protein family members 1 and 2), and WASP-family verprolin homologous protein (WAVE/SCAR) isoforms: WAVE1, WAVE2, and WAVE3. Also included are the WH2 domains found in inverted formin FH2 domain-containing protein (INF2), Cordon bleu (Cobl) protein, vasodilator-stimulated phosphoprotein (VASP) homology protein and actobindin (found in amoebae). These ABPs are commonly multidomain proteins that contain signaling domains and structurally conserved actin-binding motifs, the most important being the WH2 domain motif through which they bind actin in order to direct the location, rate, and timing for actin assembly in the cell into different structures, such as filopodia, lamellipodia, stress fibers, and focal adhesions. The WH2 domain motif is one of the most abundant actin-binding motifs in Wiskott-Aldrich syndrome proteins (WASPs) where they activate Arp2/3-dependent actin nucleation and branching in response to signals mediated by Rho-family GTPases. The thymosin beta (Tbeta) domains in metazoans act in cells as major actin-sequestering peptides; their complex with monomeric ATP-actin (G-ATP-actin) cannot polymerize at either filament (F-actin) end.


Pssm-ID: 409196  Cd Length: 22  Bit Score: 34.49  E-value: 5.48e-03
                        10        20
                ....*....|....*....|.
gi 51702526 430 SCSGRDALLDQIRQGIQLKSV 450
Cdd:cd21762   1 KTSDRSALLSDIRKGKKLKKT 21
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
Help | Disclaimer | Write to the Help Desk
NCBI | NLM | NIH