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Conserved domains on  [gi|530354695|ref|NP_001268917|]
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SPOC domain-containing protein 1 isoform 3 [Homo sapiens]

Protein Classification

Graphical summary

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List of domain hits

 Name Accession Description Interval E-value
SPOC_SPOCD1 cd21540
SPOC (Spen paralog and ortholog C-terminal) domain found in SPOC domain-containing protein 1 ...
 359-496 1.55e-80

SPOC (Spen paralog and ortholog C-terminal) domain found in SPOC domain-containing protein 1 (SPOCD1) and similar proteins; SPOCD1 is a protein belonging to the transcription factor S-II (TFIIS) family of transcription factors. It acts as a tumor-related factor that promotes cell proliferation and metastasis. SPOCD1 was initially found to interact with testis protein phosphatase 1, which is a major eukaryotic serine/threonine-specific phosphatase that regulates cellular signaling. The model corresponds to the SPOC domain of SPOCD1; the SPOC domain is involved in developmental signaling and has also been proposed to be a phosphorylation binding module.


:

Pssm-ID: 439203  Cd Length: 138  Bit Score: 251.97  E-value: 1.55e-80
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 530354695 359 PWEGVLDMFSIKRFRARAQLVSGHSCRLVQALPTVIRSAGCIPSNIVWDLLASICPAKAKDVCVVRLCPHGARDTQNCRL 438
Cdd:cd21540    1 LWEGAIQMFSIKQFGAKAYLVSGHSSQLIQALPAVIRSRGCILPESVWDYLDSIWPAEAKEMCLVRFAPAGARDSQNYRM 80

                         90       100       110       120       130
                 ....*....|....*....|....*....|....*....|....*....|....*...
gi 530354695 439 LYSYLNDRQRHGLASVEHMGMVLLPLPAFQPLPTRLRPLGGPGLEVTHSSLLLAVLLP 496
Cdd:cd21540   81 LYSYLNNKQRYGIVDSEKMDMFLIPLPAFQPVPAKLRPLGGPGLEATHSSLLLALILP 138
TFIIS_M pfam07500
Transcription factor S-II (TFIIS), central domain; Transcription elongation by RNA polymerase ...
 101-211 6.22e-31

Transcription factor S-II (TFIIS), central domain; Transcription elongation by RNA polymerase II is regulated by the general elongation factor TFIIS. This factor stimulates RNA polymerase II to transcribe through regions of DNA that promote the formation of stalled ternary complexes. TFIIS is composed of three structural domains, termed I, II, and III. The two C-terminal domains (II and III), this domain and pfam01096 are required for transcription activity.


:

Pssm-ID: 462184  Cd Length: 112  Bit Score: 116.92  E-value: 6.22e-31
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 530354695  101 VRGTVVRSMQEVLWTRlrelpDPVLSEEVVEGIAAGIEAALWDLTQGTNGRYKTKYRSLLFNLRDPRNLDLFLKVVHGDV 180
Cdd:pfam07500   5 VRDKCRELLYDALAKD-----STEASEEDALELAVEIEEALFKKFGGTNKKYKNKIRSLLFNLKDKKNPDLRRRVLSGEI 79

                          90       100       110
                  ....*....|....*....|....*....|.
gi 530354695  181 TPYDLVRMSSMQLAPQELARWRDQEEKRGLN 211
Cdd:pfam07500  80 SPEKLVTMSPEEMASEELKKEREKIEKEALK 110
 
Name Accession Description Interval E-value
SPOC_SPOCD1 cd21540
SPOC (Spen paralog and ortholog C-terminal) domain found in SPOC domain-containing protein 1 ...
 359-496 1.55e-80

SPOC (Spen paralog and ortholog C-terminal) domain found in SPOC domain-containing protein 1 (SPOCD1) and similar proteins; SPOCD1 is a protein belonging to the transcription factor S-II (TFIIS) family of transcription factors. It acts as a tumor-related factor that promotes cell proliferation and metastasis. SPOCD1 was initially found to interact with testis protein phosphatase 1, which is a major eukaryotic serine/threonine-specific phosphatase that regulates cellular signaling. The model corresponds to the SPOC domain of SPOCD1; the SPOC domain is involved in developmental signaling and has also been proposed to be a phosphorylation binding module.


Pssm-ID: 439203  Cd Length: 138  Bit Score: 251.97  E-value: 1.55e-80
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 530354695 359 PWEGVLDMFSIKRFRARAQLVSGHSCRLVQALPTVIRSAGCIPSNIVWDLLASICPAKAKDVCVVRLCPHGARDTQNCRL 438
Cdd:cd21540    1 LWEGAIQMFSIKQFGAKAYLVSGHSSQLIQALPAVIRSRGCILPESVWDYLDSIWPAEAKEMCLVRFAPAGARDSQNYRM 80

                         90       100       110       120       130
                 ....*....|....*....|....*....|....*....|....*....|....*...
gi 530354695 439 LYSYLNDRQRHGLASVEHMGMVLLPLPAFQPLPTRLRPLGGPGLEVTHSSLLLAVLLP 496
Cdd:cd21540   81 LYSYLNNKQRYGIVDSEKMDMFLIPLPAFQPVPAKLRPLGGPGLEATHSSLLLALILP 138
SPOC pfam07744
SPOC domain; The SPOC (Spen paralogue and orthologue C-terminal) domain is involved in ...
 353-497 1.21e-31

SPOC domain; The SPOC (Spen paralogue and orthologue C-terminal) domain is involved in developmental signalling.


Pssm-ID: 400205  Cd Length: 142  Bit Score: 120.15  E-value: 1.21e-31
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 530354695  353 ALPCLPPWEGVLDMFSIKRFRARAQLVSGHSCRLvqaLPTVIRSAGCIPSNIVWDLLASICPAKAKDVCVVRLCPHGARD 432
Cdd:pfam07744   2 LQDLEVIWQGTLAMKGVAEFSVRAHLVSGDIDSL---LPSLLRITGRIRLDAVWKYLDEVRRSITRDVLVVRFFPSSESD 78

                          90       100       110       120       130       140
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*...
gi 530354695  433 TQNCRLLYSYLNDRQRHGLA---SVEHMGMVLLPLPAFqplPTRLRPlGGPGLEVTHSSLLLAVLLPK 497
Cdd:pfam07744  79 ESAFDELIDYLQSKQRAGVIhakSADVKDLYLFPPCEF---LELLLP-VGLSLEVSEPNLLLGVVVRK 142
TFIIS_M pfam07500
Transcription factor S-II (TFIIS), central domain; Transcription elongation by RNA polymerase ...
 101-211 6.22e-31

Transcription factor S-II (TFIIS), central domain; Transcription elongation by RNA polymerase II is regulated by the general elongation factor TFIIS. This factor stimulates RNA polymerase II to transcribe through regions of DNA that promote the formation of stalled ternary complexes. TFIIS is composed of three structural domains, termed I, II, and III. The two C-terminal domains (II and III), this domain and pfam01096 are required for transcription activity.


Pssm-ID: 462184  Cd Length: 112  Bit Score: 116.92  E-value: 6.22e-31
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 530354695  101 VRGTVVRSMQEVLWTRlrelpDPVLSEEVVEGIAAGIEAALWDLTQGTNGRYKTKYRSLLFNLRDPRNLDLFLKVVHGDV 180
Cdd:pfam07500   5 VRDKCRELLYDALAKD-----STEASEEDALELAVEIEEALFKKFGGTNKKYKNKIRSLLFNLKDKKNPDLRRRVLSGEI 79

                          90       100       110
                  ....*....|....*....|....*....|.
gi 530354695  181 TPYDLVRMSSMQLAPQELARWRDQEEKRGLN 211
Cdd:pfam07500  80 SPEKLVTMSPEEMASEELKKEREKIEKEALK 110
TFS2M smart00510
Domain in the central regions of transcription elongation factor S-II (and elsewhere);
105-204 1.53e-23

Domain in the central regions of transcription elongation factor S-II (and elsewhere);


Pssm-ID: 128786 [Multi-domain]  Cd Length: 102  Bit Score: 95.46  E-value: 1.53e-23
                           10        20        30        40        50        60        70        80
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 530354695   105 VVRSMQEVLWTRLRELPDPVLSEEVVEGIAAGIEAALWDLTQGTNGRYKTKYRSLLFNLRDPRNLDLFLKVVHGDVTPYD 184
Cdd:smart00510   3 VRDKCQEMLYKALQKISDPEEIELDPTELAVQIEAEMFSEFGTTDKKYKNKYRSLYFNLKDKKNPDLRRKVLNGEITPEK 82

                           90       100
                   ....*....|....*....|
gi 530354695   185 LVRMSSMQLAPQELARWRDQ 204
Cdd:smart00510  83 LATMTAEELASAELKEKREK 102
TFSII TIGR01385
transcription elongation factor S-II; This model represents eukaryotic transcription ...
 65-229 8.26e-15

transcription elongation factor S-II; This model represents eukaryotic transcription elongation factor S-II. This protein allows stalled RNA transcription complexes to perform a cleavage of the nascent RNA and restart at the newly generated 3-prime end.


Pssm-ID: 273592 [Multi-domain]  Cd Length: 299  Bit Score: 75.65  E-value: 8.26e-15
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 530354695   65 PACSQSGPMEAEEDSLPEQPEDSAQLqqekPSLYI---GVRGTVVRSMQEVLWTRLRELPDPVLSEEVvegiAAGIEAAL 141
Cdd:TIGR01385 103 EAKSQSDKIEQPKYVSSSPRNAKNDF----VPTAVtndKVRDKCRELLYDALAKDSDHPPQSIDPEAK----AIQIEELK 174

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 530354695  142 WDLTQGTNGRYKTKYRSLLFNLRDPRNLDLFLKVVHGDVTPYDLVRMSSMQLAPQELARWRDQEEKRglNIIEQQQKEPC 221
Cdd:TIGR01385 175 FNNLGTTEAAYKARYRSIYSNLRDKNNPDLRHNVLTGEITPEKLATMTAEEMASAELKQEREEITKE--NLFEAQGAKIQ 252


                  ....*...
gi 530354695  222 RLPASKMT 229
Cdd:TIGR01385 253 KAVTDLFT 260
 
Name Accession Description Interval E-value
SPOC_SPOCD1 cd21540
SPOC (Spen paralog and ortholog C-terminal) domain found in SPOC domain-containing protein 1 ...
 359-496 1.55e-80

SPOC (Spen paralog and ortholog C-terminal) domain found in SPOC domain-containing protein 1 (SPOCD1) and similar proteins; SPOCD1 is a protein belonging to the transcription factor S-II (TFIIS) family of transcription factors. It acts as a tumor-related factor that promotes cell proliferation and metastasis. SPOCD1 was initially found to interact with testis protein phosphatase 1, which is a major eukaryotic serine/threonine-specific phosphatase that regulates cellular signaling. The model corresponds to the SPOC domain of SPOCD1; the SPOC domain is involved in developmental signaling and has also been proposed to be a phosphorylation binding module.


Pssm-ID: 439203  Cd Length: 138  Bit Score: 251.97  E-value: 1.55e-80
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 530354695 359 PWEGVLDMFSIKRFRARAQLVSGHSCRLVQALPTVIRSAGCIPSNIVWDLLASICPAKAKDVCVVRLCPHGARDTQNCRL 438
Cdd:cd21540    1 LWEGAIQMFSIKQFGAKAYLVSGHSSQLIQALPAVIRSRGCILPESVWDYLDSIWPAEAKEMCLVRFAPAGARDSQNYRM 80

                         90       100       110       120       130
                 ....*....|....*....|....*....|....*....|....*....|....*...
gi 530354695 439 LYSYLNDRQRHGLASVEHMGMVLLPLPAFQPLPTRLRPLGGPGLEVTHSSLLLAVLLP 496
Cdd:cd21540   81 LYSYLNNKQRYGIVDSEKMDMFLIPLPAFQPVPAKLRPLGGPGLEATHSSLLLALILP 138
SPOC_SF cd21520
SPOC (Spen paralog and ortholog C-terminal) domain superfamily; The SPOC domain is involved in ...
 359-495 1.32e-61

SPOC (Spen paralog and ortholog C-terminal) domain superfamily; The SPOC domain is involved in developmental signalling and has also been proposed to be a phosphorylation binding module. It has been found mainly in two protein families: transcription factor S-II (TFIIS) and Spen (split end). The TFIIS family includes SPOC domain-containing protein 1 (SPOCD1), yeast bypass of ESS1 protein 1 (Bye1p), PHD finger protein 3 (PHF3), and death-inducer obliterator (Dido) splicing variants, among others. They are characterized by having both a central RNA polymerase II (Pol II)-binding TFIIS-like domain (TLD) domain, and a C-terminal SPOC domain. The Spen protein family includes SMART/HDAC1-associated repressor protein (SHARP) and RNA binding motif protein 15 (RBM15)-like proteins from metazoans, as well as plant flowering time control protein FPA and yeast chromo domain-containing protein 1 (Chp1p). They are characterized by containing RNA recognition motifs (RRMs) and a SPOC domain.


Pssm-ID: 439200  Cd Length: 138  Bit Score: 202.13  E-value: 1.32e-61
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 530354695 359 PWEGVLDMFSIKRFRARAQLVSGHSCRLVQALPTVIRSAGCIPSNIVWDLLASICPAKAKDVCVVRLCPHGARDTQNCRL 438
Cdd:cd21520    1 VWQGLLALKNDPTAAARLHFVSGNNVLALSELPPVLRIAQRMRLNATQLEGVARRMAVATDYCLVLALPCGRDDESLKAA 80

                         90       100       110       120       130
                 ....*....|....*....|....*....|....*....|....*....|....*...
gi 530354695 439 LYSYLNDRQRHGLASVEHMGMVLLPLPAFQPLPTRLRPLG-GPGLEVTHSSLLLAVLL 495
Cdd:cd21520   81 FITYLQAKQRAGIASNQPAYVLQLFPPCEFSESHLSRLAPdLLASIVTISPHLMIVIL 138
SPOC pfam07744
SPOC domain; The SPOC (Spen paralogue and orthologue C-terminal) domain is involved in ...
 353-497 1.21e-31

SPOC domain; The SPOC (Spen paralogue and orthologue C-terminal) domain is involved in developmental signalling.


Pssm-ID: 400205  Cd Length: 142  Bit Score: 120.15  E-value: 1.21e-31
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 530354695  353 ALPCLPPWEGVLDMFSIKRFRARAQLVSGHSCRLvqaLPTVIRSAGCIPSNIVWDLLASICPAKAKDVCVVRLCPHGARD 432
Cdd:pfam07744   2 LQDLEVIWQGTLAMKGVAEFSVRAHLVSGDIDSL---LPSLLRITGRIRLDAVWKYLDEVRRSITRDVLVVRFFPSSESD 78

                          90       100       110       120       130       140
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*...
gi 530354695  433 TQNCRLLYSYLNDRQRHGLA---SVEHMGMVLLPLPAFqplPTRLRPlGGPGLEVTHSSLLLAVLLPK 497
Cdd:pfam07744  79 ESAFDELIDYLQSKQRAGVIhakSADVKDLYLFPPCEF---LELLLP-VGLSLEVSEPNLLLGVVVRK 142
TFIIS_M pfam07500
Transcription factor S-II (TFIIS), central domain; Transcription elongation by RNA polymerase ...
 101-211 6.22e-31

Transcription factor S-II (TFIIS), central domain; Transcription elongation by RNA polymerase II is regulated by the general elongation factor TFIIS. This factor stimulates RNA polymerase II to transcribe through regions of DNA that promote the formation of stalled ternary complexes. TFIIS is composed of three structural domains, termed I, II, and III. The two C-terminal domains (II and III), this domain and pfam01096 are required for transcription activity.


Pssm-ID: 462184  Cd Length: 112  Bit Score: 116.92  E-value: 6.22e-31
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 530354695  101 VRGTVVRSMQEVLWTRlrelpDPVLSEEVVEGIAAGIEAALWDLTQGTNGRYKTKYRSLLFNLRDPRNLDLFLKVVHGDV 180
Cdd:pfam07500   5 VRDKCRELLYDALAKD-----STEASEEDALELAVEIEEALFKKFGGTNKKYKNKIRSLLFNLKDKKNPDLRRRVLSGEI 79

                          90       100       110
                  ....*....|....*....|....*....|.
gi 530354695  181 TPYDLVRMSSMQLAPQELARWRDQEEKRGLN 211
Cdd:pfam07500  80 SPEKLVTMSPEEMASEELKKEREKIEKEALK 110
SPOC_TFIIS cd21538
SPOC (Spen paralog and ortholog C-terminal) domain found in the transcription factor S-II ...
 360-494 2.10e-28

SPOC (Spen paralog and ortholog C-terminal) domain found in the transcription factor S-II (TFIIS) family; The transcription factor S-II (TFIIS) family includes SPOC domain-containing protein 1 (SPOCD1), yeast bypass of ESS1 protein 1 (Bye1p), PHD finger protein 3 (PHF3), and death-inducer obliterator (Dido) splicing variants, among others. They are characterized by having both a central RNA polymerase II (Pol II)-binding TFIIS-like domain (TLD) domain, and a C-terminal SPOC domain. This model corresponds to the SPOC domain that is involved in developmental signaling. SPOCD1 acts as a tumor-related factor that promotes cell proliferation and metastasis. Yeast Bye1p is a nuclear transcription factor with a domain resembling the central domain in transcription elongation factor TFIIS and plays an inhibitory role during transcription elongation. It functions as a multicopy suppressor of Ess1, a peptidyl-prolyl cis-trans isomerase involved in proline isomerization of the C-terminal domain (CTD) of RNA polymerase II (Pol II). PHF3 is a human homolog of the yeast protein Bye1p. It is ubiquitously expressed in normal tissues including brain, but its expression is significantly reduced or lost in glioblastomas. The Dido/DIDO1 gene is implicated in several cellular processes such as apoptosis and chromosomal segregation, particularly in the hematopoietic system. This model corresponds to the SPOC domain that is involved in developmental signaling and has also been proposed to be a phosphorylation binding module.


Pssm-ID: 439201  Cd Length: 146  Bit Score: 110.82  E-value: 2.10e-28
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 530354695 360 WEGVLDMFSIKRFRARAQLVSGHSCR---LVQALPTVIRSAGCIPSNIVWDLLASICPAKAKDVCVVRLCPHGAR-DTQN 435
Cdd:cd21538    2 WSGKLTMPGVASFPASARQVGGPDLSstwWTDLLPSTLEIKGRIPLDKAEKYLCELRFSSSRDVVVVSLEPPDSSsDKAA 81

                         90       100       110       120       130       140
                 ....*....|....*....|....*....|....*....|....*....|....*....|...
gi 530354695 436 CRLLYSYLNDRQRHGLASVEHMG---MVLLPLPAFQPLPTRLRPL-GGPGLEVTHSSLLLAVL 494
Cdd:cd21538   82 FDELFDYFVSRDRYGVVGPKGLAvkdLYLVPLPAGDPLPPFLLLLdDGPGPEPRDEDLLLGVI 144
SPOC_PHF3 cd21548
SPOC (Spen paralog and ortholog C-terminal) domain found in PHD finger protein 3 (PHF3); PHF3 ...
 360-495 1.60e-24

SPOC (Spen paralog and ortholog C-terminal) domain found in PHD finger protein 3 (PHF3); PHF3 is a human homolog of yeast protein bypass of Ess1 (Bye1), a nuclear protein with a domain resembling the central domain in the transcription elongation factor TFIIS. It is ubiquitously expressed in normal tissues including brain, but its expression is significantly reduced or lost in glioblastomas. PHF3 contains an N-terminal plant homeodomain (PHD) finger, a central RNA polymerase II (Pol II)-binding TFIIS-like domain (TLD) domain, and a C-terminal SPOC domain. This model corresponds to the SPOC domain which is involved in developmental signaling and has also been proposed to be a phosphorylation binding module.


Pssm-ID: 439211  Cd Length: 141  Bit Score: 99.56  E-value: 1.60e-24
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 530354695 360 WEGVLDMFSIKRFRARAQLVSGHSCRLVQALPTVIRSAGCIPSNIVWDLLASICPAKAKDVCVVRLCPHGARDTQNCRLL 439
Cdd:cd21548    2 WKGFINMPSVAKFVTKAYPVSGSLEYLTEDLPDSIQVGGRISPQTVWDYVEKIKASGTKEICVVRFSPVTEEDQISYTLL 81

                         90       100       110       120       130
                 ....*....|....*....|....*....|....*....|....*....|....*....
gi 530354695 440 YSYLNDRQRHGLAS---VEHMGMVLLPLPAFQPLPTRLRPLGGPGLEVTHSSLLLAVLL 495
Cdd:cd21548   82 YAYFSSRKRYGVVAnnmKQVKDMYLIPLGASEKIPHHLVPFDGPGLELHRPNLLLGLII 140
TFS2M smart00510
Domain in the central regions of transcription elongation factor S-II (and elsewhere);
105-204 1.53e-23

Domain in the central regions of transcription elongation factor S-II (and elsewhere);


Pssm-ID: 128786 [Multi-domain]  Cd Length: 102  Bit Score: 95.46  E-value: 1.53e-23
                           10        20        30        40        50        60        70        80
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 530354695   105 VVRSMQEVLWTRLRELPDPVLSEEVVEGIAAGIEAALWDLTQGTNGRYKTKYRSLLFNLRDPRNLDLFLKVVHGDVTPYD 184
Cdd:smart00510   3 VRDKCQEMLYKALQKISDPEEIELDPTELAVQIEAEMFSEFGTTDKKYKNKYRSLYFNLKDKKNPDLRRKVLNGEITPEK 82

                           90       100
                   ....*....|....*....|
gi 530354695   185 LVRMSSMQLAPQELARWRDQ 204
Cdd:smart00510  83 LATMTAEELASAELKEKREK 102
SPOC_PPS-like cd22581
SPOC (Spen paralog and ortholog C-terminal) domain found in Drosophila melanogaster protein ...
 360-494 6.65e-23

SPOC (Spen paralog and ortholog C-terminal) domain found in Drosophila melanogaster protein partner of Sans-fille and similar proteins; Drosophila melanogaster protein partner of Sans-fille (PPS), also called protein partner of Snf, is a homolog of human DIDO. It mediates diverse chromatin activities, including the regulation of stemness genes in embryonic stem cells and splicing. PPS associates with spliceosomal RNAs including the U1 snRNP protein Sans-fille (Snf) to mediate sex determination gene Sex-lethal (Sxl) splicing autoregulation. Alternative splicing of the Sxl pre-mRNA determines gender during development in Drosophila, producing protein-encoding mRNAs in females but yielding inactive and truncated mRNAs in males. PPS contains a plant homeodomain (PHD) finger, Brahma and Kismet (BRK), a transcription elongation factor S-II subunit M (TFSIIM) domain, and a SPOC domain. This model corresponds to the SPOC domain that is involved in developmental signaling and has also been proposed to be a phosphorylation binding module.


Pssm-ID: 439214  Cd Length: 142  Bit Score: 94.98  E-value: 6.65e-23
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 530354695 360 WEGVLDMFSIKRFRARAQLVSGHSCRLVQALPTVIRSAGCIPSNIVWDLLASICPAKAKDVCVVRLCPHGARDTQNCRLL 439
Cdd:cd22581    2 WKGTINMPDVAKFNVSAQVVSGNSDDLSLDLPKVLDVVGRIAPETVWDYIGKLKKSPTKEIVVVRLTPASEEDEMSYNTF 81

                         90       100       110       120       130       140
                 ....*....|....*....|....*....|....*....|....*....|....*....|.
gi 530354695 440 YSYLNDRQRHG-LASVEhmGMV----LLPLPAFQPLPTRLRPLGGPGL-EVTHSSLLLAVL 494
Cdd:cd22581   82 FEYLSSRNRLGvIGSVS--KAIkdfyILPLPKESPIPEVLLPLDGPGLfENRRPNLLLGII 140
SPOC_DIDO1-like cd21547
SPOC (Spen paralog and ortholog C-terminal) domain found in some death-inducer obliterator ...
 360-495 1.12e-21

SPOC (Spen paralog and ortholog C-terminal) domain found in some death-inducer obliterator variants; The Dido/DIDO1 gene has been implicated in several cellular processes such as apoptosis and chromosomal segregation, particularly in the hematopoietic system. It encodes alternative splicing variants, including Dido1, 2, and 3, with Dido3 being the longest isoform and Dido1 being the shortest. Dido3 is ubiquitously expressed in all human tissues, is dispensable for embryonic stem (ES) cell self-renewal and pluripotency, but is involved in the maintenance of stem cell genomic stability and tumorigenesis. Dido3 contains a PHD finger, a transcription elongation factor S-II subunit M (TFSIIM) domain, a SPOC module, and a long C-terminal region (CT) of unknown homology. Dido2 and Dido1 are truncated at the C-terminus relative to Dido3, with Dido2 containing a partial SPOC domain whereas Dido1 is missing it completely. Dido1, also called DIO-1, or death-associated transcription factor 1 (DATF-1), is important for maintaining ES cells and directly regulates the expression of pluripotency factors. The conserved plant homeodomain (PHD) finger is responsible for the binding of histone H3 with a higher affinity for trimethylated lysine 4 (H3K4me3). The Dido/DIDO1 gene is a bone morphogenetic protein (BMP) target gene, which promotes BMP-induced melanoma progression. It also triggers apoptosis after nuclear translocation and caspase upregulation. This model corresponds to the SPOC domain which is involved in developmental signaling and has also been proposed to be a phosphorylation binding module.


Pssm-ID: 439210  Cd Length: 143  Bit Score: 91.53  E-value: 1.12e-21
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 530354695 360 WEGVLDMFSIKRFRARAQLVSGHSCRLVQALPTVIRSAGCIPSNIVWDLLASICPAKAKDVCVVRLCPHGARDTQNCRLL 439
Cdd:cd21547    2 WKGFINMQSVAKFVTKAYPVSGSFDYLSEDLPDTIHIGGRISPKTVWDYVGKLKSSLSKELCLIRFHPATEEEEVAYISL 81

                         90       100       110       120       130
                 ....*....|....*....|....*....|....*....|....*....|....*....
gi 530354695 440 YSYLNDRQRHGLAS--VEHM-GMVLLPLPAFQPLPTRLRPLGGPGLEVTHSSLLLAVLL 495
Cdd:cd21547   82 YSYFSSRGRFGVVAnnNRHVkDLYLIPLSAKDPIPSKLLPFEGPGLESTRPNIILGLVI 140
SPOC_PHF3-like cd21541
SPOC (Spen paralog and ortholog C-terminal) domain found in PHD finger protein 3 (PHF3), ...
 360-494 1.40e-21

SPOC (Spen paralog and ortholog C-terminal) domain found in PHD finger protein 3 (PHF3), death-inducer obliterator (Dido) variants, and similar proteins; PHF3 is a human homolog of yeast protein bypass of Ess1 (Bye1), a nuclear protein with a domain resembling the central domain in the transcription elongation factor TFIIS. It is ubiquitously expressed in normal tissues including brain, but its expression is significantly reduced or lost in glioblastomas. This group also includes the protein products of the Dido gene that encodes three alternative splicing variants (Dido1, 2, and 3), which have been implicated in several cellular processes such as apoptosis and chromosomal segregation, particularly in the hematopoietic system. The Dido gene is a bone morphogenetic protein (BMP) target gene and promotes BMP-induced melanoma progression. Dido1 is important for maintaining embryonic stem (ES) cells and directly regulates the expression of pluripotency factors. It is the shortest isoform that contains only a highly conserved PHD finger responsible for the binding of histone H3 with a higher affinity for trimethylated lysine4 (H3K4me3). It also triggers apoptosis after nuclear translocation and caspase upregulation. Dido3 is the largest isoform and is ubiquitously expressed in all human tissues. It is dispensable for ES cell self-renewal and pluripotency, but is involved in the maintenance of stem cell genomic stability and tumorigenesis. This model corresponds to the SPOC domain of the PHF3-like group; the SPOC domain is involved in developmental signaling and has also been proposed to be a phosphorylation binding module.


Pssm-ID: 439204  Cd Length: 141  Bit Score: 91.14  E-value: 1.40e-21
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 530354695 360 WEGVLDMFSIKRFRARAQLVSGHSCRLVQALPTVIRSAGCIPSNIVWDLLASICPAKAKDVCVVRLCPHGARDTQNCRLL 439
Cdd:cd21541    2 WKGFISMQEVAKFVASAYHVSGPCEDLSEDLPDTLEVCGRISPEQVWDYLSKLKQSGTKEIIVIRFHPANEEEKVSYISL 81

                         90       100       110       120       130
                 ....*....|....*....|....*....|....*....|....*....|....*....
gi 530354695 440 YSYLNDRQRHGLASvEHMGMV----LLPLPAFQPLPTRLRPLGGPGLEVTHSSLLLAVL 494
Cdd:cd21541   82 YSYLSSRKRCGVVG-NNSKHVkdmyLIPLASHQPIPQVLLPFDGPGLEETRPHMLLGII 139
TFSII TIGR01385
transcription elongation factor S-II; This model represents eukaryotic transcription ...
 65-229 8.26e-15

transcription elongation factor S-II; This model represents eukaryotic transcription elongation factor S-II. This protein allows stalled RNA transcription complexes to perform a cleavage of the nascent RNA and restart at the newly generated 3-prime end.


Pssm-ID: 273592 [Multi-domain]  Cd Length: 299  Bit Score: 75.65  E-value: 8.26e-15
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 530354695   65 PACSQSGPMEAEEDSLPEQPEDSAQLqqekPSLYI---GVRGTVVRSMQEVLWTRLRELPDPVLSEEVvegiAAGIEAAL 141
Cdd:TIGR01385 103 EAKSQSDKIEQPKYVSSSPRNAKNDF----VPTAVtndKVRDKCRELLYDALAKDSDHPPQSIDPEAK----AIQIEELK 174

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 530354695  142 WDLTQGTNGRYKTKYRSLLFNLRDPRNLDLFLKVVHGDVTPYDLVRMSSMQLAPQELARWRDQEEKRglNIIEQQQKEPC 221
Cdd:TIGR01385 175 FNNLGTTEAAYKARYRSIYSNLRDKNNPDLRHNVLTGEITPEKLATMTAEEMASAELKQEREEITKE--NLFEAQGAKIQ 252


                  ....*...
gi 530354695  222 RLPASKMT 229
Cdd:TIGR01385 253 KAVTDLFT 260
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
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