Nuclear transport factor 2 (NTF2) domain plays an important role in the trafficking of macromolecules, ions and small molecules between the cytoplasm and nucleus. This bi-directional transport of macromolecules across the nuclear envelope requires many soluble factors that includes GDP-binding protein Ran (RanGDP). RanGDP is required for both import and export of proteins and poly(A) RNA. RanGDP also has been implicated in cell cycle control, specifically in mitotic spindle assembly. In interphase cells, RanGDP is predominately nuclear and thought to be GTP bound, but it is also present in the cytoplasm, probably in the GDP-bound state. NTF2 mediates the nuclear import of RanGDP. NTF2 binds to both RanGDP and FxFG repeat-containing nucleoporins.

                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 238637332  13 QACRAAEEFVNIYYETMDKRRHALVRLYLDKATLIWNG-NVVTGLEALANFFEMLP--SSEFQINMLDCQPVHEQAtqcq 89
Cdd:cd00780    1 SAEDVAKAFVQQYYSIFDNNREGLHRLYGDTSMLSREGmKQVTGRDAIVEKLSSLPfqKTKHKITTVDSQPTPSGG---- 76

                         90       100       110       120
                 ....*....|....*....|....*....|....*....|....*....
gi 238637332  90 ttVLVVTSGVVKFDGNKQHFFNQNFLLtaqsTPNSTVWKIASDCFRFQD 138
Cdd:cd00780   77 --VIVMVTGSLKLDEQPPRKFSQTFVL----APQNGGYFVLNDIFRFVD 119

Nuclear transport factor 2 (NTF2) domain plays an important role in the trafficking of macromolecules, ions and small molecules between the cytoplasm and nucleus. This bi-directional transport of macromolecules across the nuclear envelope requires many soluble factors that includes GDP-binding protein Ran (RanGDP). RanGDP is required for both import and export of proteins and poly(A) RNA. RanGDP also has been implicated in cell cycle control, specifically in mitotic spindle assembly. In interphase cells, RanGDP is predominately nuclear and thought to be GTP bound, but it is also present in the cytoplasm, probably in the GDP-bound state. NTF2 mediates the nuclear import of RanGDP. NTF2 binds to both RanGDP and FxFG repeat-containing nucleoporins.

                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 238637332  13 QACRAAEEFVNIYYETMDKRRHALVRLYLDKATLIWNG-NVVTGLEALANFFEMLP--SSEFQINMLDCQPVHEQAtqcq 89
Cdd:cd00780    1 SAEDVAKAFVQQYYSIFDNNREGLHRLYGDTSMLSREGmKQVTGRDAIVEKLSSLPfqKTKHKITTVDSQPTPSGG---- 76

                         90       100       110       120
                 ....*....|....*....|....*....|....*....|....*....
gi 238637332  90 ttVLVVTSGVVKFDGNKQHFFNQNFLLtaqsTPNSTVWKIASDCFRFQD 138
Cdd:cd00780   77 --VIVMVTGSLKLDEQPPRKFSQTFVL----APQNGGYFVLNDIFRFVD 119

Nuclear transport factor 2 (NTF2) domain plays an important role in the trafficking of macromolecules, ions and small molecules between the cytoplasm and nucleus. This bi-directional transport of macromolecules across the nuclear envelope requires many soluble factors that includes GDP-binding protein Ran (RanGDP). RanGDP is required for both import and export of proteins and poly(A) RNA. RanGDP also has been implicated in cell cycle control, specifically in mitotic spindle assembly. In interphase cells, RanGDP is predominately nuclear and thought to be GTP bound, but it is also present in the cytoplasm, probably in the GDP-bound state. NTF2 mediates the nuclear import of RanGDP. NTF2 binds to both RanGDP and FxFG repeat-containing nucleoporins.

                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 238637332  13 QACRAAEEFVNIYYETMDKRRHALVRLYLDKATLIWNG-NVVTGLEALANFFEMLP--SSEFQINMLDCQPVHEQAtqcq 89
Cdd:cd00780    1 SAEDVAKAFVQQYYSIFDNNREGLHRLYGDTSMLSREGmKQVTGRDAIVEKLSSLPfqKTKHKITTVDSQPTPSGG---- 76

                         90       100       110       120
                 ....*....|....*....|....*....|....*....|....*....
gi 238637332  90 ttVLVVTSGVVKFDGNKQHFFNQNFLLtaqsTPNSTVWKIASDCFRFQD 138
Cdd:cd00780   77 --VIVMVTGSLKLDEQPPRKFSQTFVL----APQNGGYFVLNDIFRFVD 119

Nuclear transport factor 2 (NTF2-like) superfamily. This family includes members of the NTF2 family, Delta-5-3-ketosteroid isomerases, Scytalone Dehydratases, and the beta subunit of Ring hydroxylating dioxygenases. This family is a classic example of divergent evolution wherein the proteins have many common structural details but diverge greatly in their function. For example, nuclear transport factor 2 (NTF2) mediates the nuclear import of RanGDP and binds to both RanGDP and FxFG repeat-containing nucleoporins while Ketosteroid isomerases catalyze the isomerization of delta-5-3-ketosteroid to delta-4-3-ketosteroid, by intramolecular transfer of the C4-beta proton to the C6-beta position. While the function of the beta sub-unit of the Ring hydroxylating dioxygenases is not known, Scytalone Dehydratases catalyzes two reactions in the biosynthetic pathway that produces fungal melanin. Members of the NTF2-like superfamily are widely distributed among bacteria, archaea and eukaryotes.

                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 238637332  18 AEEFVNIYYETMDKR-RHALVRLYLDKATLIWNG-----NVVTGLEALANFFEMLPSSEFQINMLDcQPVHEQATQCQTT 91
Cdd:cd00531    1 AEQFLYRYARLLDAGdREWLALLYADDAYFEPPGgdgliYPDDGREAIEDRVRRLPFGPSRTRHLV-SNVDVQPGDDGEG 79

                         90       100       110       120
                 ....*....|....*....|....*....|....*....|....*....
gi 238637332  92 VLVVTSGVVKFDGNKQ---HFFNQNFLLtaqsTPNSTVWKIASDCFRFQ 137
Cdd:cd00531   80 VVVSVFGVLRTRGDGEqdvFAGGQTFVL----RPQGGGGKIANRRFRLD 124