Urate oxidase (UO, uricase) is a peroxisomal enzyme that catalyzes the oxidation of uric acid ...
187-475
5.02e-129
Urate oxidase (UO, uricase) is a peroxisomal enzyme that catalyzes the oxidation of uric acid to allantoin in most fish, amphibian, and mammalian species. The enzymatic process involves catalyzing the oxidative opening of the purine ring during the purine degradation pathway. In humans and certain other primates, however, the enzyme has been lost by some unknown mechanism. Each monomer contains two instances of this domain. Its functional form is a homotetramer for most species, though there are reports that some may form heterotetramers based on a few biochemical studies.
:
Pssm-ID: 238251 Cd Length: 286 Bit Score: 376.32 E-value: 5.02e-129
OHCU decarboxylase; Previously thought to only proceed spontaneously, the decarboxylation of ...
10-162
2.68e-84
OHCU decarboxylase; Previously thought to only proceed spontaneously, the decarboxylation of 2-oxo-4-hydroxy-4-carboxy--5-ureidoimidazoline (OHCU) has been recently been shown to be catalyzed by this enzyme in Mus musculus. Homologs of this enzyme are found adjacent to and fused with uricase in a number of prokaryotes and are represented by this model.
:
Pssm-ID: 132208 [Multi-domain] Cd Length: 157 Bit Score: 256.91 E-value: 2.68e-84
Urate oxidase (UO, uricase) is a peroxisomal enzyme that catalyzes the oxidation of uric acid ...
187-475
5.02e-129
Urate oxidase (UO, uricase) is a peroxisomal enzyme that catalyzes the oxidation of uric acid to allantoin in most fish, amphibian, and mammalian species. The enzymatic process involves catalyzing the oxidative opening of the purine ring during the purine degradation pathway. In humans and certain other primates, however, the enzyme has been lost by some unknown mechanism. Each monomer contains two instances of this domain. Its functional form is a homotetramer for most species, though there are reports that some may form heterotetramers based on a few biochemical studies.
Pssm-ID: 238251 Cd Length: 286 Bit Score: 376.32 E-value: 5.02e-129
urate oxidase; Members of this protein family are urate oxidase, also called uricase. This ...
171-475
6.32e-116
urate oxidase; Members of this protein family are urate oxidase, also called uricase. This protein contains two copies of the domain described by the uricase model pfam01014. In animals, this enzyme has been lost from primates and birds. [Central intermediary metabolism, Other]
Pssm-ID: 274554 Cd Length: 282 Bit Score: 342.64 E-value: 6.32e-116
OHCU decarboxylase; Previously thought to only proceed spontaneously, the decarboxylation of ...
10-162
2.68e-84
OHCU decarboxylase; Previously thought to only proceed spontaneously, the decarboxylation of 2-oxo-4-hydroxy-4-carboxy--5-ureidoimidazoline (OHCU) has been recently been shown to be catalyzed by this enzyme in Mus musculus. Homologs of this enzyme are found adjacent to and fused with uricase in a number of prokaryotes and are represented by this model.
Pssm-ID: 132208 [Multi-domain] Cd Length: 157 Bit Score: 256.91 E-value: 2.68e-84
OHCU decarboxylase; The proteins in this family are OHCU decarboxylase - enzymes of the purine ...
8-160
3.43e-58
OHCU decarboxylase; The proteins in this family are OHCU decarboxylase - enzymes of the purine catabolism that catalyze the conversion of OHCU into S(+)-allantoin. This is the third step of the conversion of uric acid (a purine derivative) to allantoin. Step one is catalyzed by urate oxidase (pfam01014) and step two is catalyzed by HIUases (pfam00576).
Pssm-ID: 462765 [Multi-domain] Cd Length: 156 Bit Score: 189.27 E-value: 3.43e-58
Urate oxidase (UO, uricase) is a peroxisomal enzyme that catalyzes the oxidation of uric acid ...
187-475
5.02e-129
Urate oxidase (UO, uricase) is a peroxisomal enzyme that catalyzes the oxidation of uric acid to allantoin in most fish, amphibian, and mammalian species. The enzymatic process involves catalyzing the oxidative opening of the purine ring during the purine degradation pathway. In humans and certain other primates, however, the enzyme has been lost by some unknown mechanism. Each monomer contains two instances of this domain. Its functional form is a homotetramer for most species, though there are reports that some may form heterotetramers based on a few biochemical studies.
Pssm-ID: 238251 Cd Length: 286 Bit Score: 376.32 E-value: 5.02e-129
urate oxidase; Members of this protein family are urate oxidase, also called uricase. This ...
171-475
6.32e-116
urate oxidase; Members of this protein family are urate oxidase, also called uricase. This protein contains two copies of the domain described by the uricase model pfam01014. In animals, this enzyme has been lost from primates and birds. [Central intermediary metabolism, Other]
Pssm-ID: 274554 Cd Length: 282 Bit Score: 342.64 E-value: 6.32e-116
OHCU decarboxylase; Previously thought to only proceed spontaneously, the decarboxylation of ...
10-162
2.68e-84
OHCU decarboxylase; Previously thought to only proceed spontaneously, the decarboxylation of 2-oxo-4-hydroxy-4-carboxy--5-ureidoimidazoline (OHCU) has been recently been shown to be catalyzed by this enzyme in Mus musculus. Homologs of this enzyme are found adjacent to and fused with uricase in a number of prokaryotes and are represented by this model.
Pssm-ID: 132208 [Multi-domain] Cd Length: 157 Bit Score: 256.91 E-value: 2.68e-84
OHCU decarboxylase; The proteins in this family are OHCU decarboxylase - enzymes of the purine ...
8-160
3.43e-58
OHCU decarboxylase; The proteins in this family are OHCU decarboxylase - enzymes of the purine catabolism that catalyze the conversion of OHCU into S(+)-allantoin. This is the third step of the conversion of uric acid (a purine derivative) to allantoin. Step one is catalyzed by urate oxidase (pfam01014) and step two is catalyzed by HIUases (pfam00576).
Pssm-ID: 462765 [Multi-domain] Cd Length: 156 Bit Score: 189.27 E-value: 3.43e-58
OHCU decarboxylase; Previously thought to only proceed spontaneously, the decarboxylation of ...
7-163
5.29e-16
OHCU decarboxylase; Previously thought to only proceed spontaneously, the decarboxylation of 2-oxo-4-hydroxy-4-carboxy--5-ureidoimidazoline (OHCU) has been recently been shown to be catalyzed by this enzyme in Mus musculus. Homologs of this enzyme are found adjacent to and fused with uricase in a number of prokaryotes and are represented by this model. This model is a separate (but related) clade from that represented by TIGR3164. This model places a second homolog in streptomyces species which (are not in the vicinity of other urate catabolism associated genes) below the trusted cutoff.
Pssm-ID: 188295 [Multi-domain] Cd Length: 158 Bit Score: 75.19 E-value: 5.29e-16
Tunnelling fold (T-fold). The five known T-folds are found in five different enzymes with ...
189-302
5.89e-06
Tunnelling fold (T-fold). The five known T-folds are found in five different enzymes with different functions: dihydroneopterin-triphosphate epimerase (DHNTPE), dihydroneopterin aldolase (DHNA) , GTP cyclohydrolase I (GTPCH-1), 6-pyrovoyl tetrahydropterin synthetase (PTPS), and uricase (UO,uroate/urate oxidase). They bind to substrates belonging to the purine or pterin families, and share a fold-related binding site with a glutamate or glutamine residue anchoring the substrate and a lot of conserved interactions. They also share a similar oligomerization mode: several T-folds join together to form a beta(2n)alpha(n) barrel, then two barrels join together in a head-to-head fashion to made up the native enzymes. The functional enzyme is a tetramer for UO, a hexamer for PTPS, an octamer for DHNA/DHNTPE and a decamer for GTPCH-1. The substrate is located in a deep and narrow pocket at the interface between monomers. In PTPS, the active site is located at the interface of three monomers, two from one trimer and one from the other trimer. In GTPCH-1, it is also located at the interface of three subunits, two from one pentamer and one from the other pentamer. There are four equivalent active sites in UO, six in PTPS, eight in DHNA/DHNTPE and ten in GTPCH-1. Each globular multimeric enzyme encloses a tunnel which is lined with charged residues for DHNA and UO, and with basic residues in PTPS. The N and C-terminal ends are located on one side of the T-fold while the residues involved in the catalytic activity are located at the opposite side. In PTPS, UO and DHNA/DHNTPE, the N and C-terminal extremities of the enzyme are located on the exterior side of the functional multimeric enzyme. In GTPCH-1, the extra C-terminal helix places the extremity inside the tunnel.
Pssm-ID: 238351 Cd Length: 122 Bit Score: 45.51 E-value: 5.89e-06
Database: CDSEARCH/cdd Low complexity filter: no Composition Based Adjustment: yes E-value threshold: 0.01
References:
Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
of the residues that compose this conserved feature have been mapped to the query sequence.
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