Conserved Protein Domain Family
glyc_debranch

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TIGR01531: glyc_debranch 
glycogen debranching enzymye
glycogen debranching enzyme possesses two different catalytic activities; oligo-1,4-->1,4-glucantransferase (EC 2.4.1.25) and amylo-1,6-glucosidase (EC 3.2.1.33). Site directed mutagenesis studies in S. cerevisiae indicate that the transferase and glucosidase activities are independent and located in different regions of the polypeptide chain. Proteins in this model belong to the larger alpha-amylase family. The model covers eukaryotic proteins with a seed composed of human, nematode and yeast sequences. Yeast seed sequence is well characterized. The model is quite rigorous; either query sequence yields large bit score or it fails to hit the model altogether. There doesn't appear to be any middle ground. [Energy metabolism, Biosynthesis and degradation of polysaccharides]
Statistics
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PSSM-Id: 273673
Aligned: 4 rows
Threshold Bit Score: 2309.03
Created: 8-Oct-2014
Updated: 25-Oct-2021
Structure
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Aligned Rows:
PubMed ReferencesClick to see Conserved Features Help

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
P35573      22 LFRLEQGYELQFRLGPTLQGKAVTVYTNyPFPGEtfnrekfrSLDWEnptEREDDSDKYCK-LNLQ-Q-----------S 88   human
CAB05588    22 IRKIEKGWIVRFKRGSSLLGKKVKVFTS-ICPGN--------SLEWS---EGKDHLAVYCQ-VECK-E-----------A 76   Caenorhabditis e...
NP_726062  112 LYRLKRGSKLSVHPDASLLGRKIVLYTNyPAEGQkfvrteyrVLGWQl-sNGKQITSVMHPeAHVV-DtdirsqvelnmS 189  fruit fly
CAY87144    26 LTLPPIPLPKDAPKDQPLYTVKLLVSAGsPVARDglvw-tncPPDHNtpfKRDKFYKKIIH-SSFHeDdcidl--nvyaP 101  Saccharomyces ce...
P35573      89 GSFQYYF-L-QGNEK----SGGGYIVVD-PILRVG---AdnHVLPLDCVTLQTFLAKCLGPFDEW-EsRLRVAKESGYNM 157  human
CAB05588    77 GSFRYWFkI-EDSEE----RGSGYFLVM-PELKIN---G--KCLPLDGIACQTYLTKLLGPLSEW-KeRLEVAHQTGYNM 144  Caenorhabditis e...
NP_726062  190 GTYHFYFrYlERPDTgcsgADGALYVQVePTLHVGppgAq-KTIPLDSVRCQTVLAKLLGPLDTWeP-KLRVAKEAGYNV 267  fruit fly
CAY87144   102 GSYCFYLsF-RNDNEkletTRKYYFVAL-PMLYINd-----QFLPLNSIALQSVVSKWLG--SDWeP-ILSKIAAKNYNM 171  Saccharomyces ce...
P35573     158 IHFTPLQTLGL-SRSCYSLANQLELNP-DFSRpnRKYTWNDVG--QLVEKLKKE-WNVICITDVVYNHTAANSKWIQEHP 232  human
CAB05588   145 IHLTPIHELGI-SNSSYSLSDHHSLIK-TIQSqdQKFGFEDVQ--ALVGDLERS-WNILTVQDVVWNHAAKNATWLLEHP 219  Caenorhabditis e...
NP_726062  268 IHFTPIQeLGG-SRSCYSLRDQLKVNS-HFAP--QKGGKISFEdvEKVIKKCRQeWGVASICDIVLNHTANESDWLLQHP 343  fruit fly
CAY87144   172 VHFTPLQ-ERGeSNSPYSIYDQLQFDQeHFKS--PEDVKN------LVEHIHRD-LNMLSLTDIVFNHTANNSPWLVEHP 241  Saccharomyces ce...
P35573     233 ECAYNLVNSPHLKPAWVLD--RALWRFSCDVAEgkY--KEKGIPA-LIEndH--HMNSIRKIIWEDIFPKLKLWEFFQVD 305  human
CAB05588   220 ESAYNCLNSPHLRPAYVID--RVYHEFGKQIGEgvW--EHRGVPP-VVDniH--HVNAIEYLLRAEVLPKADLHEFYQVD 292  Caenorhabditis e...
NP_726062  344 DATYSCATCPYLRPAFLLD--ATFAQCGADIAE--GslEHVGVPA-VIE--QecHLEALKYQLHTSYMSKVNIHeLYQCD 416  fruit fly
CAY87144   242 EAGYNHITAPHLISAIELDqeLLNFSRNLKSW---------GYPTeLKN--Ie-DLFKIMDGIKVHVLGSLKLWeYYAVN 309  Saccharomyces ce...
P35573     306 VNKAVE-QFRRLLTQ-EN--RRVTK-SDPNQHLTIIQDPEYRRFGCT-VDMNIAL-TTFIPHD---------KGPAAIEE 369  human
CAB05588   293 LKAMVN-LFEVFIKQ-SS--GPTTN-PLDGEDVEIEQDLECRRFGNT-VDFERSA-RIFNRHRgd-----akSEEERVEK 360  Caenorhabditis e...
NP_726062  417 VMKYVNeFMSQVRTRePP--KNVAN-ECRFQEIQLIQDPQYRRLAST-INFELAL-EIFNAFHgd-----cfDEESRFRK 486  fruit fly
CAY87144   310 VQTALR-DIKAHWND-ESneSYSFPeNIKDISSDFVKLASFVKDNVTePNFGTLGeRNSNRINvpkfiqllkLINDGGSD 387  Saccharomyces ce...
P35573     370 CC-NWFHKRMEELNsE-KHRL-IN-YHQEQA--VNCLLGNVFYERLAGHGPKLGPVTRKHPLV-TRYFTFPFEEIdfsME 442  human
CAB05588   361 CV-RSFEEALNNKNlE-AARE-SW-EVILAG--LAAVMGGITYERIADNGPKKGLVSPENPLT-TDYFLHLEADLgwkSE 433  Caenorhabditis e...
NP_726062  487 CA-ETLRRHLDALN-D-RVRCeVQ-GYINYA--IDNVLAGVRYERVQGDGPRVKEISEKHSVF-MVYFTHTGTQG---KS 556  fruit fly
CAY87144   388 DSeSSLATAQNILN-EvNLPL-YReYDDDVSeiLEQLFNRIKYLRLDDGGPKQGPVTVDVPLTePYFTRFKGKDG----- 460  Saccharomyces ce...
P35573     443 ESM--IHLPNKAC-FLMAHNGWVMG-DDPLRNFA--EPG-SEVYLRRELICWGDSVKLRYGNKPEDCPYLW-AHMKKYTE 514  human
CAB05588   434 EKF--AYDPEKSK-FLMAFNGWVMS-SDPMKNFA--LKE-SQVYLRRELVCWGDSVKLNYGNKEADSPFLW-QYMKEYTQ 505  Caenorhabditis e...
NP_726062  557 LTEieADMYTKAGeFFMAHNGWVMGySDPLRDFAeeQPGrANVYLKReLISWGDSVKLRFGRRPeDSPYLW-QHMTeYVQ 635  fruit fly
CAY87144   461 -----------TD-YALANNGWIWN-GNPLVDFA--SQN-SRAYLRReVIVWGDCVKLRYGKSPeDSPYLWeRMSK-YIE 523  Saccharomyces ce...
P35573     515 ITATYFQGVRLDNCHSTPLHVAEYMLDAARNLQPNLYVVAELFTGSEDLDNVFVTRLGISSLIREAMSAYNSHEE-GRLV 593  human
CAB05588   506 QAARIFHGLRIDNAHGTPIHVAEKLLKTAREVRPDIYVFAELFTGSEHADNMFVNRLGISSLIREAQSAHDSHEQ-GRLV 584  Caenorhabditis e...
NP_726062  636 TTARIFDGVRLDNCHSTPLHVAeYLLDAARKINPELYVVAeLFTNSDYTDNVFVNRLGITSLIReALSAWDSHeQ-GRLV 714  fruit fly
CAY87144   524 MNAKIFDGFRIDNCHSTPIHVGeYFLDLARKYNPNLYVVAeLFSGSETLDCLFVERLGISSLIReAMQAWSEE-ElSRLV 602  Saccharomyces ce...
P35573     594 YRYGGEPVGSFVQP----------------------------------CLRPLMPAIAHALFMDITHDNECPIVHRSAYD 639  human
CAB05588   585 YRYGGDCVGAFKQK----------------------------------SARLAPKSIAHGLFLDQSHDNPSPIHTRSPFD 630  Caenorhabditis e...
NP_726062  715 YRYGGVPVGGFQAN----------------------------------SSRHEATSVAHALFLDLTHDNPSPVEKRSVYD 760  fruit fly
CAY87144   603 HKHGGRPIGSYKFVpmddfsypadinlneehcfndsndnsircvseimIPKILTATPPHALFMDCTHDNETPFEKRTVED 682  Saccharomyces ce...
P35573     640 ALPSTTIVSMACCASGSTRGYDELVPHQISVVSE-ERFYTKWNPEalpSNTgevnfqSGIIAARCAISKLHQELGAK-GF 717  human
CAB05588   631 ILPTAAMLTMASCAVGSNRGYDELIRDHIHVVSE-KRPYASWCRPdqvSRS------QGIIEGRNLLNKLHTWLAEH-GY 702  Caenorhabditis e...
NP_726062  761 LLPSAALVSMACCATGSNRGYDeLVPHHIHVVDE-ERTYQEWGKG---VDSk-----SGIMGAKRALNLLHGQLAEE-GF 830  fruit fly
CAY87144   683 TLPNAALVALCSSAIGSVYGYDeIFPHLLNLVTEkRHYDISTPTG---SPS------IGITKVKATLNSIRTSIGEKaYD 753  Saccharomyces ce...
P35573     718 IQ--VYVDQVDEDIVAVTRHSPSIHQSVVAVSRTAFRNPKTSFYSk-EVPQMCIPGKIEEVVLE-ARTIERNTKPYR--- 790  human
CAB05588   703 SQ--VFVDQMNSDIVGITRHNPRTHETVVVVSHTSFSKNYIDWPG--GLKHIPIGGVLENVIFE-MKLKKVQEEWGT--- 774  Caenorhabditis e...
NP_726062  831 SQ--VYVDQMDPNVVAVTRHSPITHQSVILVAHTAFGYPSPNAGPt-GIRPLRFEGVLDEIILEaSLTMQSDKPFDRpap 907  fruit fly
CAY87144   754 IEdsEMHVHHQGQYITFHRMDVKSGKGWYLIARMKFSDND---DPneTLPPVVLNQSTCSLRFS-YALERV----GD--- 822  Saccharomyces ce...
P35573     791 --KDENSINGTPDITVEIRE--HIQLNESKIVKQAgvatKGpneyiqEIEFEN-LSPGSVIIFRVSLDPHAQVAVG-ILR 864  human
CAB05588   775 --EDPDVLIGLKNYEMEIRE--NVNLDNGTMFKVH----DG------YIELTN-FPTGSVVGFKIRPSDEATKAFN-MIH 838  Caenorhabditis e...
NP_726062  908 fkKDPNVINGFTQFQLNLQE--HIPLAKSTVFQTQay-sDGnn---tELNFAN-LRPGTVVAIRVSMHPGPRTSFD-KLQ 979  fruit fly
CAY87144   823 --EIPNDDKFIKGIPTKLKEleGFDISYDDSKKIS------------TIKLPNeFPQGSIAIFETQ-----QNGVDeSLD 883  Saccharomyces ce...
P35573     865 NHLTQFSPHFK------SgslavdnadpilkipfasLASRLTLAELNQILYRCE---SE--EKEDGG-GCYDIPNWSALK 932  human
CAB05588   839 NSITPEQSEFD------S------------------ALSRLTYQSFPNLLFHCE---SE--DYATIQqGGYDVPNFGKFV 889  Caenorhabditis e...
NP_726062  980 KISAALRIGSGeeysqlQa-----------------IVSKLDLVALSGALFSCD---DEerDLGKGG-TAYDIPNFGKIV 1038 fruit fly
CAY87144   884 HFIRSGALKAT------Ss---------------------LTLESINSVLYRSEpeeYD--VSAGEG-GAYIIPNFGKPV 933  Saccharomyces ce...
P35573     933 YAGLQGLMSVLAEIRPKNDLGHPFCNNLRSGDWMIDYVSNRLIsRSGTIA--EVGKWLQAMFFYLKQIPRYLIPCYFDAI 1010 human
CAB05588   890 YCGLQGLVPVLEKIRDDNDLGHPLCQNLRDGTWICDYIVGRLA-KFEKLG--EVSEAIRKFFAPLDHVPYYLRPCYFELL 966  Caenorhabditis e...
NP_726062 1039 YCGLQGFISLLTEISPKNDLGHPLCNNLRDGNWMMDYISDRLTsY-EDLK--PLSAWFKATFEPLKNIPRYLIPCYFDAI 1115 fruit fly
CAY87144   934 YCGLQGWVSVLRKIVFYNDLAHPLSANLRNGHWALDYTISRLNyYSDEAGinEVQNWLRSRFDRVKKLPSYLVPSYFALI 1013 Saccharomyces ce...
P35573    1011 LIGAYTTLLDTAWKQMSSFVQNGSTFVKHLSLGSVQLCGVgkfpslpILSPALMDV-PYRLneitKEKEQCCVSLAAGLP 1089 human
CAB05588   967 VSYIYGKIRKEALKRMAPQISSSSALVRHLAISTLSFLGY-------IPGAGLAPI-PTSL----QIEDQYPSSLAAGLS 1034 Caenorhabditis e...
NP_726062 1116 VSGVYNVLINQVNELMPDFIKNGHSFPQSLALSTLQFLSV-------CKSANLPGF-SPALs--pPKPPKQCVTLSAGLP 1185 fruit fly
CAY87144  1014 IGILYGCCRLKAIQLMSRNIGKSTLFVQSLSMTSIQMVSR-------MKSTSILPGeNVP-------------SMAAGLP 1073 Saccharomyces ce...
P35573    1090 HFSSGIFRCWGRDTFIALRGILLITGRYVEARNIILAFAGTLRHGLIPNLLGEGIYARYNCRDAVWWWLQCIQDYCKMVP 1169 human
CAB05588  1035 HFAVGIWRNWGRDTFIALPGCLLSTGRFQEARQIILSFAGAIRHGLIPNLLAEGIGARYNCRDATWFWLVSIVKYVESAP 1114 Caenorhabditis e...
NP_726062 1186 HFSTGYMRCWGRDTFIALRGSMFLTGRYNeARFIIIGFGQTLRHGLIPNLLDSGSKPRFNCRDAIWWWMYCIKQYVEDAP 1265 fruit fly
CAY87144  1074 HFSVNYMRCWGRDVFISLRGMLLTTGRFDeAKAHILAFAKTLKHGLIPNLLDAGRNPRYNARDAAWFFLQAVQDYVYIVP 1153 Saccharomyces ce...
P35573    1170 NGLD-ILK-CPVSRMYPTDDSAPLpAGTLDQPLF---EVIQEAMQKHMQGIQFRERNAGPQIDRNMKDEGFNITAGVDEE 1244 human
CAB05588  1115 NGVG-ILE-DPVRRIYPNDDSVYG-EGEVQQMLI---ETIYEALDKHFAGIDYRERSAGPQIDEQMRDEGFQVTAGVSRT 1188 Caenorhabditis e...
NP_726062 1266 KG-AeILK-DKVSRIFPYDDADAHaPGAFDQLLF---DVMQEALQVHFQGLQYReRNAGYEIDAHMVDQGFNNQIGIHPE 1340 fruit fly
CAY87144  1154 DGeK-ILQeQVTRRFPLDDTYIPVdDPRAFSYSSileEIIYEILSRHAKGIKFReANAGPNLDRVMTDKGFNVEIHVDWS 1232 Saccharomyces ce...
P35573    1245 TGFVYGGNRFNCGTWMDKMGESDRARNRGIPATPRDGSAVEIVGLSKSAVRWLLELSKknifpyhEVT-VKRHGK-AIKV 1322 human
CAB05588  1189 TGFIYGGNRWNCGTWMDKMGSSERAGNKGEPATPRDGAAVELQGLAYRALKSLKNWKE-------QGV-IQRSGV-SDEW 1259 Caenorhabditis e...
NP_726062 1341 TGFVFGGNNFNCGTWMDKMGSSQKAGNKGRPSTPRDGSAVeLVGLQYAVLRFMQSLAEkev-ipyTGVeRKGPSGeVTKW 1419 fruit fly
CAY87144  1233 TGLIHGGSQYNCGTWMDKMGESEKAGSVGIPGTPRDGAAIeINGLLKSALRFVIELKN-------KGL-FKFSDVeTQDG 1304 Saccharomyces ce...
P35573    1323 SYD---EWNRKIQDNFEKLFHV--SEDPsdl-nekHPNLVHKRGIYKDSYGASSPWCDYQLRPNFTIAMVVAPELFTTEK 1396 human
CAB05588  1260 TWG---FWAQKIRENFEKEFFV--DKDS-------YAEFVNRREIIKDSVGSTLGFTDFQLRCNFGIALAVAPDLMDPKK 1327 Caenorhabditis e...
NP_726062 1420 SYK---EWADRIKNNFDKYFFV--SESEtc-------sVANKKLIYKDSYGATQSWTDYQLRCNFPITLTVAPDLCNPQN 1487 fruit fly
CAY87144  1305 GRIdftEWNQLLQDNFEKRYYVpeDPSQdadydvsAKLGVNRRGIYRDLYKSGKPYEDYQLRPNFAIAMTVAPELFVPEH 1384 Saccharomyces ce...
P35573    1397 AWKAL-EIA-EKkLLGPLGMKTLDPDDMVYCGIYDNALDNDNYNLAKGFNYHQGPEWLWPIGYFLRAKLYFSRLMGPETT 1474 human
CAB05588  1328 AWKAL-DSA-EV-LLGPLGMKTLDPTDWAYNGYYNNDDDGTDKKTAKGWNYHQGPEWLFVAGYYLQARLRIGDILGGSEK 1404 Caenorhabditis e...
NP_726062 1488 AWRALeRAK-KY-LLGPLGMKTMDPEDWNYRANYDNSNDSTDCTVAHGANYHQGPeWVWPIGFYLRARLIFAKKCGHLDE 1565 fruit fly
CAY87144  1385 AIKAI-TIAdEV-LRGPVGMRTLDPSDYNYRPYYNNGEDSDDFATSKGRNYHQGPeWVWLYGYFLRAFHHFHFKTSPRCQ 1462 Saccharomyces ce...
P35573    1475 --AKTI-VLVKNVLSRHYVHLE-------RSPWKGLPELTNENAQYCPFSCETQAWSIATILETLYDL 1532 human
CAB05588  1405 --QYAI-RQVQERLGNAYKHII-------SSPWRSLPELTNADGEYCMQSCAAQAWSVGCLIEACVKL 1462 Caenorhabditis elegans
NP_726062 1566 tiAETW-AILRAHLRE----LQ-------TSHWRGLPeLTNDNGSYCGDSCRTQAWSVAAILEVLYDL 1621 fruit fly
CAY87144  1463 --NAAKeKPSSYLYQQLYYRLKghrkwifESVWAGLTeLTNKDGEVCNDSSPTQAWSSACLLDLFYDL 1528 Saccharomyces cerevisiae EC1118
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