1UOX


Conserved Protein Domain Family
Uricase
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cd00445: Uricase 
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Urate oxidase (UO, uricase) is a peroxisomal enzyme that catalyzes the oxidation of uric acid to allantoin in most fish, amphibian, and mammalian species. The enzymatic process involves catalyzing the oxidative opening of the purine ring during the purine degradation pathway. In humans and certain other primates, however, the enzyme has been lost by some unknown mechanism. Each monomer contains two instances of this domain. Its functional form is a homotetramer for most species, though there are reports that some may form heterotetramers based on a few biochemical studies.
Links
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Statistics
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PSSM-Id: 238251
Aligned: 12 rows
Threshold Bit Score: 376.32
Created: 6-Mar-2002
Updated: 2-Oct-2020
Structure
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Program:
Drawing:
Aligned Rows:
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active sitetetramer
Conserved site includes 8 residues -Click on image for an interactive view with Cn3D
Feature 1:active site [active site]
Evidence:
  • Comment:each monomer partially contributes to two active sites present in the dimer
  • Citation:PMID 9360612
  • Structure:1UOX: binds 8-azaxanthine, a competitive inhibitor
    - View structure with Cn3D
  • Comment:a carboxyl group in uric acid is replaced with a nitrogen to form 8-azaxanthine
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Sequence Alignment
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Format: Row Display: Color Bits: Type Selection: 
Feature 1                                                        ##                           
1UOX        8 RYGKDNVRVYKVHKDEk-tGVQTVYEMTVCVLLEG-EIETSYTKADNSVIVATDSIKNTIYITAKQNP-VTPPELFGSIL 84  Aspergillus flavus
2501644     9 TYGKDNVKFLKVKKDPqnpKKQEVMEATVTCLLEG-GFDTSYTEADNSSIVPTDTVKNTILVLAKTTE-IWPIERFAAKL 86  Pichia jadinii
137103     32 GYGKDAVKVMHINRKG---PVHSIQELEVGTHLKL-YSNKDYMLGNNSDVVATDSQKNTVYLLAKKHG-IESPEKFALIL 106 Drosophila virilis
137104     20 GYGKDMVKVLHIQRDG---KYHSIKEVATSVQLTL-RSKKDYLHGDNSDIIPTDTIKNTVHVLAKLRG-IRNIETFAMNI 94  house mouse
6136148    21 TYLKPLTGVRTIPESPfsgRDHILFGVNVKISVGGtKLLTSFTKGDNSLVVATDSMKNFIQKHLASYT-GTTIEGFLEYV 99  Bacillus sp. TB-90
6136149   187 TYLKPLTGVKQIPESSfagRDNTVVGVDVTCEIGGeAFLPSFTDGDNTLVVATDSMKNFIQRHLASYE-GTTTEGFLHYV 265 Bacillus subtilis
6136152    15 RHGKERVRVARVWKTRq--GQHFVVEWRVGITLFS-DCVNSYLRDDNSDIVATDTMKNTVYAKAKECSdILSAEDFAILL 91  soybean
6175090    13 RHGKARVRVGRVWRHAh-dGSHHFVEWNVSISLLS-HCLSSYRLDDNSDIVATDTIKNTVYVKAKECGdRLSVEEFAILI 90  thale cress
AAF10733   22 NYGKAEVQLMKIKRGT---PRHELREAKVRVAMYG-DFGAAHSEGDNTDLVATDTVRNTVYGLAKEGF-ESSIEEFGKEL 96  Deinococcus radiod...
AAG28465   10 HHGKSKVRLGRVWRDG---TVHHMVEWNVNTMIDS-DMEHAFLNGDNTGMTATDTQKNTVYVVAQRMSqKCTIEQYAIAL 85  Chlamydomonas rein...
14286179   38 GYGKDAVKVLHVSRNG---PVHAIQEFEVGTHLKL-YSKKDYYQGNNSDIVATDSQKNTVYLLAKKHG-IESPEKFALLL 112 fruit fly
BAB63819   10 RHGKSRVRVSRVWRRPaaaGGHVIVEWNVAVSVVS-DCLPSYTSDDNSAIVATDSIKNTVYVKAKECTeIVSMEEFAVIL 88  Oryza sativa (japo...

Feature 1                                                                                     
1UOX       85 GTHFIEKYNHIHAAHVNIVCHRWTRMDIDGKP-------------------------HPHSFIRDseEKRNVQVDVVEG- 138 Aspergillus flavus
2501644    87 ATHFVEKYSHVSGVSVKIVQDRWVKYAVDGKP-------------------------HDHSFIHEggEKRITDLYYKRS- 140 Pichia jadinii
137103    107 AKHFLSTYAHVEEVHVHVEAYPWQRMTQDVSDnigkgycennc----nsrsngncqlHNHAFIFTptAHDYCDVILTRQd 182 Drosophila virilis
137104     95 CEHFLSSFNHVTRAHVYVEEVPWKRFEKNGIK-------------------------HVHAFIHTptGTHFCEVEQMRN- 148 house mouse
6136148   100 ATSFLKKYSHIEKISLIGEEIPFETTFAVKNGnr---------------------aaSELVFKKSrnEYATAYLNMVRNe 158 Bacillus sp. TB-90
6136149   266 AHRFLDTYSHMDTITLTGEDIPFEAMPAYEEKel---------------------stSRLVFRRSrnERSRSVLKAERSg 324 Bacillus subtilis
6136152    92 AKHFVSFYKKVTGAIVNIVEKPWERVIVDGQP-------------------------HEHGFKLGs-EKHTTEAIVQKS- 144 soybean
6175090    91 GKHFCSFYPQVFTAIVNIIEKPWERVSIDGKP-------------------------HLHGFKLGs-ENHTTEARVEKS- 143 thale cress
AAF10733   97 LTHFVKVGPRVTGGFAEFTEHLWERVQTPAQPq-----------------------gHDHAFVRQm-PKRTARVETQDG- 151 Deinococcus radiod...
AAG28465   86 AQHFVRTYPLVSKAKIYVEQKPWTRVQQSGLP-------------------------HDHGFALQgtEVRTVYVTYDKA- 139 Chlamydomonas rein...
14286179  113 ARHFINKYSHVEEAHVHVEAYPWQRVCQEETRtnvngkcengvqgncdfssidnrslHNHAFIFTptALHYCDVVIRRTd 192 fruit fly
BAB63819   89 GRHFTSLYPQVSEATVTIAERPWERVVVDGKP-------------------------HSHGFKLGv-EKHVTEVIVKKS- 141 Oryza sativa (japo...

Feature 1                              #                 #                                    
1UOX      139 ----KGIDIKSSLSGLTVLKSTNSQFWGFLRDE-YTTLKETWDRILSTDVDATWQWKnfsglqe----vrshvpKFDATW 209 Aspergillus flavus
2501644   141 ----GDYKLSSAIKDLTVLKSTGSMFYGYNKCD-FTTLQPTTDRILSTDVDATWVWDnkkigsvydiakaadkgIFDNVY 215 Pichia jadinii
137103    183 ----PKQTVISGIKGLRVLKTTQSSFVNFVDDE-FRTLADQYDRIFSTVVECSWEYSdte------------svNFLHAW 245 Drosophila virilis
137104    149 ----GPPVIHSGIKDLKVLKTTQSGFEGFLKDQ-FTTLPEVKDRCFATQVYCKWRYQrr-------------dvDFEAIW 210 house mouse
6136148   159 dntlNITEQQSGLAGLQLIKVSGNSFVGFIRDE-YTTLPEDSNRPLFVYLNIKWKYKnteds-------fgtnpENYVAA 230 Bacillus sp. TB-90
6136149   325 n-tiTITEQYSEIMDLQLVKVSGNSFVGFIRDE-YTTLPEDGNRPLFVYLNISWQYEntnds-------yasdpARYVAA 395 Bacillus subtilis
6136152   145 ----GSLQLTSGIEGLSVLKTTQSGFVNFIRDK-YTALPDTRERILATEVTALWRYSyesqys-----lpqkplYFTEKY 214 soybean
6175090   144 ----GALNLTSGIGGLALLKTTQSGFERFVRDK-YTILPETRERMLATEVNASWRYSyesvas-----iptkglYFSEKF 213 thale cress
AAF10733  152 ----RRFTVTSGIEELYVLKTTESGWENYLLDErFTTLPETHDRVMATFVTAKWEYAve-------------scDYDAVW 214 Deinococcus radiod...
AAG28465  140 ----GTLDVTAGLKDLSLLKTTQSGYEGFLRDQ-YTLLPETNDRIMASTVTCTWKYAaa-------------paCYDAAY 201 Chlamydomonas rein...
14286179  193 ----PKQTVITGIKGLRVLKTTQSSFVNFVNDE-FRSLPDQYDRIFSTVVDCSWEYSdte------------nlDFLRAW 255 fruit fly
BAB63819  142 ----GNLLINSGIQGYSLLKTTQSGFEKFVRDR-YTLLPDTRERIVATEVTAWWRYPfehvsq-----ipsksfCFTQRY 211 Oryza sativa (japo...

Feature 1                           # #                         # #                           
1UOX      210 ATAREVTLKTFAED-----NSASVQATMYKMAEQILARQQLIETVEYSLPNKHYFEIDLSWHkglqntgknaeVFAPQSD 284 Aspergillus flavus
2501644   216 NQAREITLTTFALE-----NSPSVQATMFNMATQILEKACSVYSVSYALPNKHYFLIDLKWKglen----dneLFYPSPH 286 Pichia jadinii
137103    246 ETVKDIVVRNFAGDpsvgiPSPSVQHTLYLSEKQVLDVLPQVSVVSMTMPNKHYFNFDTKPFqqlvp-genneVFIPTDK 324 Drosophila virilis
137104    211 GAVRDIVLQKFAGPydkgeYSPSVQKTLYDIQVLSLSQLPEIEDMEISLPNIHYFNIDMSKMglin----keeVLLPLDN 286 house mouse
6136148   231 EQIRDIATSVFHET-----ETLSIQHLIYLIGRRILERFPQLQEVYFESQNHTWDKIVEEIPese------gkVYTEPRP 299 Bacillus sp. TB-90
6136149   396 EQVRDLASTVFHEL-----ETPSIQNLIYHIGCRILARFPQLTDVSFQSQNHTWDTVVEEIPgsk------gkVYTEPRP 464 Bacillus subtilis
6136152   215 QEVKKVLADTFFGPpnggvYSPSVQNTLYLMAKATLNRFPDIAYVSLKMPNLHFLPVNISNKdgpiv-kfeddVYLPTDE 293 soybean
6175090   214 MDVKKVLMDTFFGPpetgvYSPSVQRTLYLMGSAVLKRFADVSSIHLKMPNIHFLPVNLSTKenpsmvkfkddVYLPTDE 293 thale cress
AAF10733  215 ERVYRQIQHTFTDH-----YSPSLQRTLYLMGEAVLSVCPEISRIWFQMPNKHHLVYNLGRFglen----nneILHVDPE 285 Deinococcus radiod...
AAG28465  202 AAAKQGLLDAFLGPgarrrVQPSVQYTLYDMAKNILDRVPTSESIFLNMPNLHFIPCNPVGSsfn------ndVYVATSE 275 Chlamydomonas rein...
14286179  256 QTVKNIIIRNFAGDpqvgvSSPSVQHTLYLSERQVLDVLPQVSVISMTMPNKHYFNFDTKPFqkiap-gdnneVFIPVDK 334 fruit fly
BAB63819  212 QDVKKVLADTFFGPpdvgvYSPSVQNTLYLMAKEVLNRFPDIASVQLRMPNLHFIPVNLGNKenpglvkfaddVYLPTDE 291 Oryza sativa (japo...

Feature 1                
1UOX      285 PNGLIKCTVGR 295 Aspergillus flavus
2501644   287 PNGLIKCTVVR 297 Pichia jadinii
137103    325 PHGTIYAQLSR 335 Drosophila virilis
137104    287 PYGKITGTVKR 297 house mouse
6136148   300 PYGFQCFTVTQ 310 Bacillus sp. TB-90
6136149   465 PYGFQHFTVTR 475 Bacillus subtilis
6136152   294 PHGSIQASLSR 304 soybean
6175090   294 PHGSIEATLSR 304 thale cress
AAF10733  286 PYGLMEAWVER 296 Deinococcus radiodurans
AAG28465  276 PHGNIEAVITR 286 Chlamydomonas reinhardtii
14286179  335 PHGTIYAQLAR 345 fruit fly
BAB63819  292 PHGTIEATVSR 302 Oryza sativa (japonica cultivar-group)

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