2UCZ,1AYZ,1C4Z,1FBV,1FXT,1J7D,1QCQ,2AAK,2E2C


Conserved Protein Domain Family
UBCc
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cd00195: UBCc (this model, PSSM-Id:238117 is obsolete and has been replaced by 467407)
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Ubiquitin-conjugating enzyme E2, catalytic (UBCc) domain. This is part of the ubiquitin-mediated protein degradation pathway in which a thiol-ester linkage forms between a conserved cysteine and the C-terminus of ubiquitin and complexes with ubiquitin protein ligase enzymes, E3. This pathway regulates many fundamental cellular processes. There are also other E2s which form thiol-ester linkages without the use of E3s as well as several UBC homologs (TSG101, Mms2, Croc-1 and similar proteins) which lack the active site cysteine essential for ubiquitination and appear to function in DNA repair pathways which were omitted from the scope of this CD.
Links
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Statistics
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PSSM-Id: 238117
Aligned: 70 rows
Threshold Bit Score: 93.8061
Created: 1-Nov-2000
Updated: 2-Oct-2020
Structure
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Program:
Drawing:
Aligned Rows:
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Conserved site include 1 residue -Click on image for an interactive view with Cn3D
Feature 1:active site cysteine
Evidence:
  • Comment:forms thiol-ester with ubiquitin or ubiquitin-like proteins; others (TSG101, Mms2) lack this cysteine and appear to function in DNA repair pathways
  • Comment:serves as the point of attachment of ubiquitin prior to transfer to cellular target
  • Citation:PMID 9497353
  • Citation:PMID 9705497
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Sequence Alignment
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Format: Row Display: Color Bits: Type Selection: 
Feature 1                                                                                     
2UCZ        6 QKRLLKELQQLIKDSp--pGI-VAGPKSenNIFIWDCLIQGp----pDTPY-----------ADGVFNAKLEFPKDYPLS 67  baker's yeast
1FXT_A      3 AKRIMKEIQAVKDDPa--aHI-TLEFVSesDIHHLKGTFLGp----pGTPY-----------EGGKFVVDIEVPMEYPFK 64  baker's yeast
1749704     1 YKRLMKEYLALQKNPf--eLV-DAKPATe-NILEWHYIITGp----pDTPY-----------EGGQYHGTLIFPPDYPFK 61  fission yeast
7498090     7 VRRLMKEAMELRQPTe---MY-HAQPMEd-NLFEWHFTIRGt----lGTDF-----------EGGIYHGRIIFPADYPMK 66  Caenorhabditis ele...
7499834   216 TDRLMKEIRDIHRSEhfknGIyTFELEKeeNLYQWWIKLHKvd---eDSPLfedmkklkkdhNQDHLLFSFTFNEKFPCD 292 Caenorhabditis ele...
NP_057105  19 VKRLMKEAAELKDPTd---HY-HAHALEd-NLFEWHFTVRGp----pDSDF-----------DGGVYHGRIVLPPEYPMK 78  human
CAB89584    8 IKRLSNEYRRLQKPEnrvrEY-YVAPLAd-NIFEWHFTLRGpggddnSLPY-----------KKGIYHGALIFSRSYPLE 74  Leishmania major
NP_199854   7 IKRLQKEYRALCKEPv--sHV-VARPSPn-DILEWHYVLEGs----eGTPF-----------AGGFYYGKIKFPPEYPYK 67  thale cress
NP_494397  21 VRRLQKDYAKLMQDPv--dGI-KALPNEd-NILEWHYCLRGs----pDTPF-----------YGGYYWGKVIFKENFPWS 81  Caenorhabditis ele...
NP_566563  13 VKRILQEVKEMQANPs--dDF-MSLPLEe-NIFEWQFAIRGp----gDTEF-----------EGGIYHGRIQLPADYPFK 73  thale cress

Feature 1                            #                                                        
2UCZ       68 PPKLTFt-pSILHPNIY-PNGEVCISILHSpgddpnmyelaeerWSPVQSVEKILLSVMSMLS--EPNi-ESGAN----- 137 baker's yeast
1FXT_A     65 PPKMQFd-tKVYHPNISsVTGAICLDILKNa-------------WSPVITLKSALISLQALLQ--SPEp-NDPQD----- 122 baker's yeast
1749704    62 PPAIRM---ITPSGRFQ-TNTRLCLSFSDFhp----------ksWNPSWMVSTILVGLVSFMTsdEITtgGIVTSes--t 125 fission yeast
7498090    67 PPNLIL---LTPNGRFE-LNKKVCLSISGYhp----------etWLPSWSIRTALLALIGFLPs-TPGg-ALGSLdy--p 128 Caenorhabditis ele...
7499834   293 PPFVRVvapHINQGFVL-GGGAICMELLTKq------------gWSSAYSIESCILQIAATLVk-GRAriSFDAKh---- 354 Caenorhabditis ele...
NP_057105  79 PPSIIL---LTANGRFE-VGKKICLSISGHhp----------etWQPSWSIRTALLAIIGFMPt-KGEg-AIGSLdy--t 140 human
CAB89584   75 PPDILF---FTRSGRFA-VREKICSTISSYhk----------elWQPTYDIALTLTALRHFMAq-EDEf-GVGAFpknmi 138 Leishmania major
NP_199854  68 PPGITM---TTPNGRFV-TQKKICLSMSDFhp----------esWNPMWSVSSILTGLLSFMMdnSPTtgSVNTSva--e 131 thale cress
NP_494397  82 PPAITM---ITPNGRFQ-TNTRLCLSISDYhp----------esWNPGWTVSAILIGLHSFMNenSPAagSIAGTpq--d 145 Caenorhabditis ele...
NP_566563  74 PPSFML---LTPNGRFE-TNTKICLSISNYhp----------ehWQPSWSVRTALVALIAFMPt-SPNg-ALGSVdy--p 135 thale cress

Feature 1                                
2UCZ      138 -IDACILWRd----nRPEFERQVKLSI 159 baker's yeast
1FXT_A    123 -AEVAQHYLr----dRESFNKTAALWT 144 baker's yeast
1749704   126 rRTYAKDTKrfnimdNPKFLIMFPELI 152 fission yeast
7498090   129 pKERQRLAKl-----SCEWKCKECGCV 150 Caenorhabditis elegans
7499834   355 -TSTYSMARa-----QQSFKSLQQIHA 375 Caenorhabditis elegans
NP_057105 141 pEERRALAKk-----SQDFCCEGCGSA 162 human
CAB89584  139 aRETKEAWAt----eTWSFTCSTCGMA 161 Leishmania major
NP_199854 132 kQRLAKSSLafn-ckSVTFRKLFPEYV 157 thale cress
NP_494397 146 qRMYAAASKefn-vkVYNWNLAGNPHC 171 Caenorhabditis elegans
NP_566563 136 kDERRTLAIk----sRETPPKYGSPER 158 thale cress

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